Derivation of human triploid trophoblast stem cells
Purpose Human trophoblast stem cells (hTSCs) are counterparts of the precursor cells of the placenta and are valuable cell models for the study of placental development and the pathogenesis of placental diseases. The aim of this work was to establish a triploid human TSC (hTSC 3PN ) derived from the...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 2022-05, Vol.39 (5), p.1183-1193 |
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| container_title | Journal of assisted reproduction and genetics |
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| creator | Kong, Xuhui Chen, Xin Ou, Songbang Wang, Wenjun Li, Ruiqi |
| description | Purpose
Human trophoblast stem cells (hTSCs) are counterparts of the precursor cells of the placenta and are valuable cell models for the study of placental development and the pathogenesis of placental diseases. The aim of this work was to establish a triploid human TSC (hTSC
3PN
) derived from the tripronuclear embryos, which are clinically discarded but readily available, for potential applications in basic placental research and disease modeling.
Methods
Eighteen tripronuclear human zygotes from IVF were collected and cultured for 5–6 days. Five high-quality blastocysts were harvested and were individually cultured in hTSC medium. Finally, two hTSC lines were established after 10 days and could be passaged stably.
Results
The karyotyping analysis showed that hTSC
3PN
contained three sets of chromosomes. And the hTSC
3PN
exhibited typical features of hTSCs, with the ability to differentiate into two trophoblast lineages: extravillous cytotrophoblasts (EVTs) and syncytiotrophoblasts (STs). In addition, the hTSC
3PN
can mimic some vital features of trophoblast, including hormone secretion and invasion. Further studies showed that the proliferation and differentiation of hTSC
3PN
were reduced compared with normal hTSCs, which may be related to the disturbed metabolic signaling in hTSC
3PN
.
Conclusions
We established the triploid hTSC lines derived from tripronuclear embryos, which provides a potentially useful research model in vitro to study human placental biology and diseases. |
| doi_str_mv | 10.1007/s10815-022-02436-w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9107551</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2664203874</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-c12749252892f2f5b7e59dc925a47128e1c0cb3f7115ecc43c8419b010279f703</originalsourceid><addsrcrecordid>eNp9kctOwzAQRS0EoqXwAyxQJDZsAuNXnGyQUHlKldjA2nJcp02VxMVOWvH3uKSUx4KF5ZHnzB1fXYROMVxiAHHlMaSYx0BIOIwm8XoPDTEXNBaUwn6ogacxsCQdoCPvFwCQpYQeogHlgecChojeGleuVFvaJrJFNO9q1UStK5eVLaehsMu5zSvl28i3po60qSp_jA4KVXlzsr1H6PX-7mX8GE-eH57GN5NYM8HaWGMiWEY4STNSkILnwvBsqsOLYgKT1GANOqeFwJgbrRnVKcNZDhiIyAoBdISue91ll9dmqk3TOlXJpStr5d6lVaX83WnKuZzZlcwwCM5xELjYCjj71hnfyrr0GwuqMbbzkiQ0wQwYJwE9_4MubOeaYC9QCSNAU8ECRXpKO-u9M8XuMxjkJhPZZyJDJvIzE7kOQ2c_bexGvkIIAO0BH1rNzLjv3f_IfgBcI5a8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2664203874</pqid></control><display><type>article</type><title>Derivation of human triploid trophoblast stem cells</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Kong, Xuhui ; Chen, Xin ; Ou, Songbang ; Wang, Wenjun ; Li, Ruiqi</creator><creatorcontrib>Kong, Xuhui ; Chen, Xin ; Ou, Songbang ; Wang, Wenjun ; Li, Ruiqi</creatorcontrib><description>Purpose
Human trophoblast stem cells (hTSCs) are counterparts of the precursor cells of the placenta and are valuable cell models for the study of placental development and the pathogenesis of placental diseases. The aim of this work was to establish a triploid human TSC (hTSC
3PN
) derived from the tripronuclear embryos, which are clinically discarded but readily available, for potential applications in basic placental research and disease modeling.
Methods
Eighteen tripronuclear human zygotes from IVF were collected and cultured for 5–6 days. Five high-quality blastocysts were harvested and were individually cultured in hTSC medium. Finally, two hTSC lines were established after 10 days and could be passaged stably.
Results
The karyotyping analysis showed that hTSC
3PN
contained three sets of chromosomes. And the hTSC
3PN
exhibited typical features of hTSCs, with the ability to differentiate into two trophoblast lineages: extravillous cytotrophoblasts (EVTs) and syncytiotrophoblasts (STs). In addition, the hTSC
3PN
can mimic some vital features of trophoblast, including hormone secretion and invasion. Further studies showed that the proliferation and differentiation of hTSC
3PN
were reduced compared with normal hTSCs, which may be related to the disturbed metabolic signaling in hTSC
3PN
.
Conclusions
We established the triploid hTSC lines derived from tripronuclear embryos, which provides a potentially useful research model in vitro to study human placental biology and diseases.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/s10815-022-02436-w</identifier><identifier>PMID: 35243570</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Blastocysts ; Cell culture ; Cell Differentiation - genetics ; Chromosomes ; Embryos ; Female ; Genetics ; Gynecology ; Human Genetics ; Humans ; Medicine ; Medicine & Public Health ; Placenta ; Pregnancy ; Reproductive Medicine ; Stem Cells ; Triploidy ; Trophoblasts - metabolism ; Zygotes</subject><ispartof>Journal of assisted reproduction and genetics, 2022-05, Vol.39 (5), p.1183-1193</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-c12749252892f2f5b7e59dc925a47128e1c0cb3f7115ecc43c8419b010279f703</citedby><cites>FETCH-LOGICAL-c474t-c12749252892f2f5b7e59dc925a47128e1c0cb3f7115ecc43c8419b010279f703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107551/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107551/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51298,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35243570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kong, Xuhui</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Ou, Songbang</creatorcontrib><creatorcontrib>Wang, Wenjun</creatorcontrib><creatorcontrib>Li, Ruiqi</creatorcontrib><title>Derivation of human triploid trophoblast stem cells</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><addtitle>J Assist Reprod Genet</addtitle><description>Purpose
Human trophoblast stem cells (hTSCs) are counterparts of the precursor cells of the placenta and are valuable cell models for the study of placental development and the pathogenesis of placental diseases. The aim of this work was to establish a triploid human TSC (hTSC
3PN
) derived from the tripronuclear embryos, which are clinically discarded but readily available, for potential applications in basic placental research and disease modeling.
Methods
Eighteen tripronuclear human zygotes from IVF were collected and cultured for 5–6 days. Five high-quality blastocysts were harvested and were individually cultured in hTSC medium. Finally, two hTSC lines were established after 10 days and could be passaged stably.
Results
The karyotyping analysis showed that hTSC
3PN
contained three sets of chromosomes. And the hTSC
3PN
exhibited typical features of hTSCs, with the ability to differentiate into two trophoblast lineages: extravillous cytotrophoblasts (EVTs) and syncytiotrophoblasts (STs). In addition, the hTSC
3PN
can mimic some vital features of trophoblast, including hormone secretion and invasion. Further studies showed that the proliferation and differentiation of hTSC
3PN
were reduced compared with normal hTSCs, which may be related to the disturbed metabolic signaling in hTSC
3PN
.
Conclusions
We established the triploid hTSC lines derived from tripronuclear embryos, which provides a potentially useful research model in vitro to study human placental biology and diseases.</description><subject>Blastocysts</subject><subject>Cell culture</subject><subject>Cell Differentiation - genetics</subject><subject>Chromosomes</subject><subject>Embryos</subject><subject>Female</subject><subject>Genetics</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Placenta</subject><subject>Pregnancy</subject><subject>Reproductive Medicine</subject><subject>Stem Cells</subject><subject>Triploidy</subject><subject>Trophoblasts - metabolism</subject><subject>Zygotes</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctOwzAQRS0EoqXwAyxQJDZsAuNXnGyQUHlKldjA2nJcp02VxMVOWvH3uKSUx4KF5ZHnzB1fXYROMVxiAHHlMaSYx0BIOIwm8XoPDTEXNBaUwn6ogacxsCQdoCPvFwCQpYQeogHlgecChojeGleuVFvaJrJFNO9q1UStK5eVLaehsMu5zSvl28i3po60qSp_jA4KVXlzsr1H6PX-7mX8GE-eH57GN5NYM8HaWGMiWEY4STNSkILnwvBsqsOLYgKT1GANOqeFwJgbrRnVKcNZDhiIyAoBdISue91ll9dmqk3TOlXJpStr5d6lVaX83WnKuZzZlcwwCM5xELjYCjj71hnfyrr0GwuqMbbzkiQ0wQwYJwE9_4MubOeaYC9QCSNAU8ECRXpKO-u9M8XuMxjkJhPZZyJDJvIzE7kOQ2c_bexGvkIIAO0BH1rNzLjv3f_IfgBcI5a8</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Kong, Xuhui</creator><creator>Chen, Xin</creator><creator>Ou, Songbang</creator><creator>Wang, Wenjun</creator><creator>Li, Ruiqi</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220501</creationdate><title>Derivation of human triploid trophoblast stem cells</title><author>Kong, Xuhui ; Chen, Xin ; Ou, Songbang ; Wang, Wenjun ; Li, Ruiqi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-c12749252892f2f5b7e59dc925a47128e1c0cb3f7115ecc43c8419b010279f703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Blastocysts</topic><topic>Cell culture</topic><topic>Cell Differentiation - genetics</topic><topic>Chromosomes</topic><topic>Embryos</topic><topic>Female</topic><topic>Genetics</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Placenta</topic><topic>Pregnancy</topic><topic>Reproductive Medicine</topic><topic>Stem Cells</topic><topic>Triploidy</topic><topic>Trophoblasts - metabolism</topic><topic>Zygotes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Xuhui</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Ou, Songbang</creatorcontrib><creatorcontrib>Wang, Wenjun</creatorcontrib><creatorcontrib>Li, Ruiqi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Xuhui</au><au>Chen, Xin</au><au>Ou, Songbang</au><au>Wang, Wenjun</au><au>Li, Ruiqi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Derivation of human triploid trophoblast stem cells</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><stitle>J Assist Reprod Genet</stitle><addtitle>J Assist Reprod Genet</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>39</volume><issue>5</issue><spage>1183</spage><epage>1193</epage><pages>1183-1193</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><abstract>Purpose
Human trophoblast stem cells (hTSCs) are counterparts of the precursor cells of the placenta and are valuable cell models for the study of placental development and the pathogenesis of placental diseases. The aim of this work was to establish a triploid human TSC (hTSC
3PN
) derived from the tripronuclear embryos, which are clinically discarded but readily available, for potential applications in basic placental research and disease modeling.
Methods
Eighteen tripronuclear human zygotes from IVF were collected and cultured for 5–6 days. Five high-quality blastocysts were harvested and were individually cultured in hTSC medium. Finally, two hTSC lines were established after 10 days and could be passaged stably.
Results
The karyotyping analysis showed that hTSC
3PN
contained three sets of chromosomes. And the hTSC
3PN
exhibited typical features of hTSCs, with the ability to differentiate into two trophoblast lineages: extravillous cytotrophoblasts (EVTs) and syncytiotrophoblasts (STs). In addition, the hTSC
3PN
can mimic some vital features of trophoblast, including hormone secretion and invasion. Further studies showed that the proliferation and differentiation of hTSC
3PN
were reduced compared with normal hTSCs, which may be related to the disturbed metabolic signaling in hTSC
3PN
.
Conclusions
We established the triploid hTSC lines derived from tripronuclear embryos, which provides a potentially useful research model in vitro to study human placental biology and diseases.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>35243570</pmid><doi>10.1007/s10815-022-02436-w</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1058-0468 |
| ispartof | Journal of assisted reproduction and genetics, 2022-05, Vol.39 (5), p.1183-1193 |
| issn | 1058-0468 1573-7330 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9107551 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Blastocysts Cell culture Cell Differentiation - genetics Chromosomes Embryos Female Genetics Gynecology Human Genetics Humans Medicine Medicine & Public Health Placenta Pregnancy Reproductive Medicine Stem Cells Triploidy Trophoblasts - metabolism Zygotes |
| title | Derivation of human triploid trophoblast stem cells |
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