Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis
Background Chemoresistance is one of the major obstacles for tumor treatment. Circular RNAs (circRNAs) have been confirmed to play vital roles in chemoresistance of cancer, including esophageal squamous cell carcinoma (ESCC). We investigated the roles and mechanisms of circ_0007142 in cisplatin (DDP...
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| Veröffentlicht in: | Journal of clinical laboratory analysis 2022-05, Vol.36 (5), p.e24304-n/a |
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| description | Background
Chemoresistance is one of the major obstacles for tumor treatment. Circular RNAs (circRNAs) have been confirmed to play vital roles in chemoresistance of cancer, including esophageal squamous cell carcinoma (ESCC). We investigated the roles and mechanisms of circ_0007142 in cisplatin (DDP) resistance of ESCC.
Methods
Quantitative real‐time polymerase chain reaction (qRT‐PCR) was conducted to determine the levels of circ_0007142, DOCK1 mRNA, microRNA‐494‐3p (miR‐494‐3p) and LIM And SH3 Protein 1 (LASP1) mRNA. RNase R assay was conducted to analyze the characteristic of circ_0007142. Cell Counting Kit‐8 (CCK‐8) assay was performed to evaluate IC50 of DDP. Flow cytometry analysis, 5‐ethynyl‐2’‐deoxyuridine (EdU) assay and transwell assay were carried out to examine cell apoptosis, proliferation and invasion, respectively. Dual‐luciferase reporter assay was employed to verify the association between miR‐494‐3p and circ_0007142 or LASP1. Murine xenograft assay was conducted to investigate the role of circ_0007142 in DDP resistant in vivo. The protein level of LASP1 in tumors was measured by Immunohistochemistry (IHC) analysis.
Results
Circ_0007142 was upregulated in DDP‐resistant ESCC tissues and cells. Circ_0007142 knockdown improved DDP sensitivity, induced cell apoptosis and hampered cell proliferation and invasion in DDP‐resistant ESCC cells. Circ_0007142 functioned as the sponge for miR‐494‐3p and miR‐494‐3p inhibition reversed the impacts of circ_0007142 knockdown on DDP resistance, cell apoptosis, proliferation, and invasion. LASP1 was a target of miR‐494‐3p, and the effects on DDP resistance, cell apoptosis, growth, and invasion mediated by LASP1 downregulation were rescued by miR‐494‐3p inhibition. Moreover, circ_0007142 knockdown enhanced DDP sensitivity in vivo.
Conclusion
Circ_0007142 improved DDP resistance of ESCC by upregulating LASP1 via sponging miR‐494‐3p.
Circ_0007142 promoted DDP resistance, cell proliferation, and invasion in DDP‐resistant ESCC cells by regulating miR‐494‐3p and LASP1. |
| doi_str_mv | 10.1002/jcla.24304 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9102771</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2641518183</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5144-debb584eaaa0e462439a9f5768a8b5ac9eeca8b27321dd2f409646aec2416713</originalsourceid><addsrcrecordid>eNp9kcFu1DAQhi1ERZfChQdAlrigSmk9jpM4F6TVqqVUK4Ggd2vinW29SuLUTkp76yPwjDwJ3m6pgAOX8Vjz6df_62fsDYgjEEIeb2yLR1LlQj1jMxC1zqSWxXM2E1pXmRaQ77OXMW6EELqG8gXbz4scJEAxY7dnEY11wZp0rUBJbn0_BtdMI0U-em5dHFocXc8DRRdH7C3x9KPohyu8JGx5vJ6w81PkltqWWwzW9b5DfuOQd-7rz_sfqlZp5sPxcv7tC3C8dfEV21tjG-n143vALk5PLhZn2fLzx0-L-TKzBSiVrahpCq0IEQWpMqWssV4XValRNwXamsimTVa5hNVKrpWoS1UiWamgrCA_YB92ssPUdLSylMJha4bgOgx3xqMzf196d2Uu_Y2pQcjqQeD9o0Dw1xPF0XQuboNiTymzkaWCAjToPKHv_kE3fgp9SpeoMleQPFaJOtxRNvgYA62fzIAw2z7Ntk_z0GeC3_5p_wn9XWACYAd8dy3d_UfKnC-W853oLxQdrMQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2663413217</pqid></control><display><type>article</type><title>Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Online Library Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Chang, Na ; Ge, Ning ; Zhao, Yufei ; Yang, Liu ; Qin, Wei ; Cui, Yayun</creator><creatorcontrib>Chang, Na ; Ge, Ning ; Zhao, Yufei ; Yang, Liu ; Qin, Wei ; Cui, Yayun</creatorcontrib><description>Background
Chemoresistance is one of the major obstacles for tumor treatment. Circular RNAs (circRNAs) have been confirmed to play vital roles in chemoresistance of cancer, including esophageal squamous cell carcinoma (ESCC). We investigated the roles and mechanisms of circ_0007142 in cisplatin (DDP) resistance of ESCC.
Methods
Quantitative real‐time polymerase chain reaction (qRT‐PCR) was conducted to determine the levels of circ_0007142, DOCK1 mRNA, microRNA‐494‐3p (miR‐494‐3p) and LIM And SH3 Protein 1 (LASP1) mRNA. RNase R assay was conducted to analyze the characteristic of circ_0007142. Cell Counting Kit‐8 (CCK‐8) assay was performed to evaluate IC50 of DDP. Flow cytometry analysis, 5‐ethynyl‐2’‐deoxyuridine (EdU) assay and transwell assay were carried out to examine cell apoptosis, proliferation and invasion, respectively. Dual‐luciferase reporter assay was employed to verify the association between miR‐494‐3p and circ_0007142 or LASP1. Murine xenograft assay was conducted to investigate the role of circ_0007142 in DDP resistant in vivo. The protein level of LASP1 in tumors was measured by Immunohistochemistry (IHC) analysis.
Results
Circ_0007142 was upregulated in DDP‐resistant ESCC tissues and cells. Circ_0007142 knockdown improved DDP sensitivity, induced cell apoptosis and hampered cell proliferation and invasion in DDP‐resistant ESCC cells. Circ_0007142 functioned as the sponge for miR‐494‐3p and miR‐494‐3p inhibition reversed the impacts of circ_0007142 knockdown on DDP resistance, cell apoptosis, proliferation, and invasion. LASP1 was a target of miR‐494‐3p, and the effects on DDP resistance, cell apoptosis, growth, and invasion mediated by LASP1 downregulation were rescued by miR‐494‐3p inhibition. Moreover, circ_0007142 knockdown enhanced DDP sensitivity in vivo.
Conclusion
Circ_0007142 improved DDP resistance of ESCC by upregulating LASP1 via sponging miR‐494‐3p.
Circ_0007142 promoted DDP resistance, cell proliferation, and invasion in DDP‐resistant ESCC cells by regulating miR‐494‐3p and LASP1.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.24304</identifier><identifier>PMID: 35312115</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Animals ; Apoptosis ; Cancer therapies ; Cell growth ; Cell proliferation ; Cell Proliferation - genetics ; Chemoresistance ; Chemotherapy ; Cholecystokinin ; circ_0007142 ; Cisplatin ; Cisplatin - pharmacology ; Cisplatin - therapeutic use ; Cytoskeletal Proteins - genetics ; DDP ; Drug dosages ; Drug resistance ; Drug Resistance, Neoplasm - genetics ; ESCC ; Esophageal cancer ; Esophageal Neoplasms - drug therapy ; Esophageal Neoplasms - genetics ; Esophageal Squamous Cell Carcinoma - drug therapy ; Esophageal Squamous Cell Carcinoma - genetics ; Flow cytometry ; Humans ; Immunohistochemistry ; LASP1 ; LIM Domain Proteins - genetics ; LIM Domain Proteins - metabolism ; Lung Neoplasms - genetics ; Medical prognosis ; Metastasis ; Mice ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; miR‐494‐3p ; mRNA ; Ovarian cancer ; Polymerase chain reaction ; Proteins ; resistance ; Ribonuclease ; RNA, Messenger ; Squamous cell carcinoma ; Tumors ; Xenografts</subject><ispartof>Journal of clinical laboratory analysis, 2022-05, Vol.36 (5), p.e24304-n/a</ispartof><rights>2022 The Authors. published by Wiley Periodicals LLC</rights><rights>2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5144-debb584eaaa0e462439a9f5768a8b5ac9eeca8b27321dd2f409646aec2416713</citedby><cites>FETCH-LOGICAL-c5144-debb584eaaa0e462439a9f5768a8b5ac9eeca8b27321dd2f409646aec2416713</cites><orcidid>0000-0001-7009-3765</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102771/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102771/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,1412,11543,27905,27906,45555,45556,46033,46457,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35312115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Na</creatorcontrib><creatorcontrib>Ge, Ning</creatorcontrib><creatorcontrib>Zhao, Yufei</creatorcontrib><creatorcontrib>Yang, Liu</creatorcontrib><creatorcontrib>Qin, Wei</creatorcontrib><creatorcontrib>Cui, Yayun</creatorcontrib><title>Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis</title><title>Journal of clinical laboratory analysis</title><addtitle>J Clin Lab Anal</addtitle><description>Background
Chemoresistance is one of the major obstacles for tumor treatment. Circular RNAs (circRNAs) have been confirmed to play vital roles in chemoresistance of cancer, including esophageal squamous cell carcinoma (ESCC). We investigated the roles and mechanisms of circ_0007142 in cisplatin (DDP) resistance of ESCC.
Methods
Quantitative real‐time polymerase chain reaction (qRT‐PCR) was conducted to determine the levels of circ_0007142, DOCK1 mRNA, microRNA‐494‐3p (miR‐494‐3p) and LIM And SH3 Protein 1 (LASP1) mRNA. RNase R assay was conducted to analyze the characteristic of circ_0007142. Cell Counting Kit‐8 (CCK‐8) assay was performed to evaluate IC50 of DDP. Flow cytometry analysis, 5‐ethynyl‐2’‐deoxyuridine (EdU) assay and transwell assay were carried out to examine cell apoptosis, proliferation and invasion, respectively. Dual‐luciferase reporter assay was employed to verify the association between miR‐494‐3p and circ_0007142 or LASP1. Murine xenograft assay was conducted to investigate the role of circ_0007142 in DDP resistant in vivo. The protein level of LASP1 in tumors was measured by Immunohistochemistry (IHC) analysis.
Results
Circ_0007142 was upregulated in DDP‐resistant ESCC tissues and cells. Circ_0007142 knockdown improved DDP sensitivity, induced cell apoptosis and hampered cell proliferation and invasion in DDP‐resistant ESCC cells. Circ_0007142 functioned as the sponge for miR‐494‐3p and miR‐494‐3p inhibition reversed the impacts of circ_0007142 knockdown on DDP resistance, cell apoptosis, proliferation, and invasion. LASP1 was a target of miR‐494‐3p, and the effects on DDP resistance, cell apoptosis, growth, and invasion mediated by LASP1 downregulation were rescued by miR‐494‐3p inhibition. Moreover, circ_0007142 knockdown enhanced DDP sensitivity in vivo.
Conclusion
Circ_0007142 improved DDP resistance of ESCC by upregulating LASP1 via sponging miR‐494‐3p.
Circ_0007142 promoted DDP resistance, cell proliferation, and invasion in DDP‐resistant ESCC cells by regulating miR‐494‐3p and LASP1.</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cancer therapies</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Chemoresistance</subject><subject>Chemotherapy</subject><subject>Cholecystokinin</subject><subject>circ_0007142</subject><subject>Cisplatin</subject><subject>Cisplatin - pharmacology</subject><subject>Cisplatin - therapeutic use</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>DDP</subject><subject>Drug dosages</subject><subject>Drug resistance</subject><subject>Drug Resistance, Neoplasm - genetics</subject><subject>ESCC</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - drug therapy</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Squamous Cell Carcinoma - drug therapy</subject><subject>Esophageal Squamous Cell Carcinoma - genetics</subject><subject>Flow cytometry</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>LASP1</subject><subject>LIM Domain Proteins - genetics</subject><subject>LIM Domain Proteins - metabolism</subject><subject>Lung Neoplasms - genetics</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Mice</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>miR‐494‐3p</subject><subject>mRNA</subject><subject>Ovarian cancer</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>resistance</subject><subject>Ribonuclease</subject><subject>RNA, Messenger</subject><subject>Squamous cell carcinoma</subject><subject>Tumors</subject><subject>Xenografts</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFu1DAQhi1ERZfChQdAlrigSmk9jpM4F6TVqqVUK4Ggd2vinW29SuLUTkp76yPwjDwJ3m6pgAOX8Vjz6df_62fsDYgjEEIeb2yLR1LlQj1jMxC1zqSWxXM2E1pXmRaQ77OXMW6EELqG8gXbz4scJEAxY7dnEY11wZp0rUBJbn0_BtdMI0U-em5dHFocXc8DRRdH7C3x9KPohyu8JGx5vJ6w81PkltqWWwzW9b5DfuOQd-7rz_sfqlZp5sPxcv7tC3C8dfEV21tjG-n143vALk5PLhZn2fLzx0-L-TKzBSiVrahpCq0IEQWpMqWssV4XValRNwXamsimTVa5hNVKrpWoS1UiWamgrCA_YB92ssPUdLSylMJha4bgOgx3xqMzf196d2Uu_Y2pQcjqQeD9o0Dw1xPF0XQuboNiTymzkaWCAjToPKHv_kE3fgp9SpeoMleQPFaJOtxRNvgYA62fzIAw2z7Ntk_z0GeC3_5p_wn9XWACYAd8dy3d_UfKnC-W853oLxQdrMQ</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Chang, Na</creator><creator>Ge, Ning</creator><creator>Zhao, Yufei</creator><creator>Yang, Liu</creator><creator>Qin, Wei</creator><creator>Cui, Yayun</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7009-3765</orcidid></search><sort><creationdate>202205</creationdate><title>Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis</title><author>Chang, Na ; Ge, Ning ; Zhao, Yufei ; Yang, Liu ; Qin, Wei ; Cui, Yayun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5144-debb584eaaa0e462439a9f5768a8b5ac9eeca8b27321dd2f409646aec2416713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cancer therapies</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Chemoresistance</topic><topic>Chemotherapy</topic><topic>Cholecystokinin</topic><topic>circ_0007142</topic><topic>Cisplatin</topic><topic>Cisplatin - pharmacology</topic><topic>Cisplatin - therapeutic use</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>DDP</topic><topic>Drug dosages</topic><topic>Drug resistance</topic><topic>Drug Resistance, Neoplasm - genetics</topic><topic>ESCC</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - drug therapy</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Squamous Cell Carcinoma - drug therapy</topic><topic>Esophageal Squamous Cell Carcinoma - genetics</topic><topic>Flow cytometry</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>LASP1</topic><topic>LIM Domain Proteins - genetics</topic><topic>LIM Domain Proteins - metabolism</topic><topic>Lung Neoplasms - genetics</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Mice</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>miR‐494‐3p</topic><topic>mRNA</topic><topic>Ovarian cancer</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>resistance</topic><topic>Ribonuclease</topic><topic>RNA, Messenger</topic><topic>Squamous cell carcinoma</topic><topic>Tumors</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Na</creatorcontrib><creatorcontrib>Ge, Ning</creatorcontrib><creatorcontrib>Zhao, Yufei</creatorcontrib><creatorcontrib>Yang, Liu</creatorcontrib><creatorcontrib>Qin, Wei</creatorcontrib><creatorcontrib>Cui, Yayun</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Na</au><au>Ge, Ning</au><au>Zhao, Yufei</au><au>Yang, Liu</au><au>Qin, Wei</au><au>Cui, Yayun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J Clin Lab Anal</addtitle><date>2022-05</date><risdate>2022</risdate><volume>36</volume><issue>5</issue><spage>e24304</spage><epage>n/a</epage><pages>e24304-n/a</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Background
Chemoresistance is one of the major obstacles for tumor treatment. Circular RNAs (circRNAs) have been confirmed to play vital roles in chemoresistance of cancer, including esophageal squamous cell carcinoma (ESCC). We investigated the roles and mechanisms of circ_0007142 in cisplatin (DDP) resistance of ESCC.
Methods
Quantitative real‐time polymerase chain reaction (qRT‐PCR) was conducted to determine the levels of circ_0007142, DOCK1 mRNA, microRNA‐494‐3p (miR‐494‐3p) and LIM And SH3 Protein 1 (LASP1) mRNA. RNase R assay was conducted to analyze the characteristic of circ_0007142. Cell Counting Kit‐8 (CCK‐8) assay was performed to evaluate IC50 of DDP. Flow cytometry analysis, 5‐ethynyl‐2’‐deoxyuridine (EdU) assay and transwell assay were carried out to examine cell apoptosis, proliferation and invasion, respectively. Dual‐luciferase reporter assay was employed to verify the association between miR‐494‐3p and circ_0007142 or LASP1. Murine xenograft assay was conducted to investigate the role of circ_0007142 in DDP resistant in vivo. The protein level of LASP1 in tumors was measured by Immunohistochemistry (IHC) analysis.
Results
Circ_0007142 was upregulated in DDP‐resistant ESCC tissues and cells. Circ_0007142 knockdown improved DDP sensitivity, induced cell apoptosis and hampered cell proliferation and invasion in DDP‐resistant ESCC cells. Circ_0007142 functioned as the sponge for miR‐494‐3p and miR‐494‐3p inhibition reversed the impacts of circ_0007142 knockdown on DDP resistance, cell apoptosis, proliferation, and invasion. LASP1 was a target of miR‐494‐3p, and the effects on DDP resistance, cell apoptosis, growth, and invasion mediated by LASP1 downregulation were rescued by miR‐494‐3p inhibition. Moreover, circ_0007142 knockdown enhanced DDP sensitivity in vivo.
Conclusion
Circ_0007142 improved DDP resistance of ESCC by upregulating LASP1 via sponging miR‐494‐3p.
Circ_0007142 promoted DDP resistance, cell proliferation, and invasion in DDP‐resistant ESCC cells by regulating miR‐494‐3p and LASP1.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>35312115</pmid><doi>10.1002/jcla.24304</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7009-3765</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 0887-8013 1098-2825 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adaptor Proteins, Signal Transducing - genetics Animals Apoptosis Cancer therapies Cell growth Cell proliferation Cell Proliferation - genetics Chemoresistance Chemotherapy Cholecystokinin circ_0007142 Cisplatin Cisplatin - pharmacology Cisplatin - therapeutic use Cytoskeletal Proteins - genetics DDP Drug dosages Drug resistance Drug Resistance, Neoplasm - genetics ESCC Esophageal cancer Esophageal Neoplasms - drug therapy Esophageal Neoplasms - genetics Esophageal Squamous Cell Carcinoma - drug therapy Esophageal Squamous Cell Carcinoma - genetics Flow cytometry Humans Immunohistochemistry LASP1 LIM Domain Proteins - genetics LIM Domain Proteins - metabolism Lung Neoplasms - genetics Medical prognosis Metastasis Mice MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA miR‐494‐3p mRNA Ovarian cancer Polymerase chain reaction Proteins resistance Ribonuclease RNA, Messenger Squamous cell carcinoma Tumors Xenografts |
| title | Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T14%3A30%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hsa_circ_0007142%20contributes%20to%20cisplatin%20resistance%20in%20esophageal%20squamous%20cell%20carcinoma%20via%20miR%E2%80%90494%E2%80%903p/LASP1%20axis&rft.jtitle=Journal%20of%20clinical%20laboratory%20analysis&rft.au=Chang,%20Na&rft.date=2022-05&rft.volume=36&rft.issue=5&rft.spage=e24304&rft.epage=n/a&rft.pages=e24304-n/a&rft.issn=0887-8013&rft.eissn=1098-2825&rft_id=info:doi/10.1002/jcla.24304&rft_dat=%3Cproquest_pubme%3E2641518183%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2663413217&rft_id=info:pmid/35312115&rfr_iscdi=true |