Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean
WRINKLED1 (WRI1), an APETALA2/ethylene-responsive-element-binding protein (AP2/EREBP) subfamily transcription factor, plays a crucial role in the transcriptional regulation of plant fatty acid biosynthesis. In this study, was overexpressed in the soybean cultivar 'Dongnong 50' using -media...
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| Veröffentlicht in: | International journal of molecular sciences 2022-05, Vol.23 (9), p.5084 |
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| creator | Wang, Zhikun Wang, Yuanzhuo Shang, Ping Yang, Chao Yang, Mingming Huang, Jinxiu Ren, Baizheng Zuo, Zhaohui Zhang, Qingyan Li, Wenbin Song, Bo |
| description | WRINKLED1 (WRI1), an APETALA2/ethylene-responsive-element-binding protein (AP2/EREBP) subfamily transcription factor, plays a crucial role in the transcriptional regulation of plant fatty acid biosynthesis. In this study,
was overexpressed in the soybean cultivar 'Dongnong 50' using
-mediated transformation to generate three transgenic lines with high seed oil contents. PCR and Southern blotting analysis showed that the T-DNA was inserted into the genome at precise insertion sites and was stably inherited by the progeny. Expression analysis using qRT-PCR and Western blotting indicated that
and
driven by the CaMV
promoter were significantly upregulated in the transgenic plants at different developmental stages. Transcriptome sequencing results showed there were obvious differences in gene expression between transgenic line and transgenic receptor during seed developmental stages. KEGG analysis found that the differentially expressed genes mainly annotated to metabolic pathways, such as carbohydrated metabolism and lipid metabolism. A 2-year single-location field trial revealed that three transgenic lines overexpressing
(GmWRI1a-OE) showed a stable increase in seed oil content of 4.97-10.35%. Importantly, no significant effect on protein content and yield was observed. Overexpression of
changed the fatty acid composition by increasing the linoleic acid (C18:2) content and decreasing the palmitic acid (C16:0) content in the seed. The three GmWRI1a-OE lines showed no significant changes in agronomic traits. The results demonstrated that the three
overexpression lines exhibited consistent increases in seed oil content compared with that of the wild type and did not significantly affect the seed yield and agronomic traits. The genetic engineering of
will be an effective strategy for the improvement of seed oil content and value in soybean. |
| doi_str_mv | 10.3390/ijms23095084 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9102168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2664786641</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-e8bfd82b0d82373a0a8017c030a73f4ba79f518ff7fd1bc3957f0e626f44eb983</originalsourceid><addsrcrecordid>eNpdkctLAzEQh4Motj5uniXgxYPVSbK72b0IUnwUCoVW8Riy24lu2U1qshX737ulD6qXyUC--ZjhR8gFg1shMrgrZ3XgArIY0uiAdFnEeQ8gkYd7fYechDAD4ILH2THpiDhORCR5l4xH3-jxZ-4xhNJZ6gyduGWO2tLn-n08YJpOGp1XSzqwhUcdMNDmE-kEcUpHZUX7zjZoG1ra7eAZOTK6Cni-eU_J29Pja_-lNxw9D_oPw14RMd70MM3NNOU5tEVIoUGnwGQBArQUJsq1zEzMUmOkmbK8EFksDWDCExNFmGepOCX3a-98kdc4LdotvK7U3Je19kvldKn-_tjyU324b5Ux4CxZCa43Au--FhgaVZehwKrSFt0iKJ4kkUzbwlr06h86cwtv2_NWlIC0Na6EN2uq8C4Ej2a3DAO1Ckvth9Xil_sH7OBtOuIXe1CP4w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2663080218</pqid></control><display><type>article</type><title>Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean</title><source>PubMed (Medline)</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>EZB Electronic Journals Library</source><creator>Wang, Zhikun ; Wang, Yuanzhuo ; Shang, Ping ; Yang, Chao ; Yang, Mingming ; Huang, Jinxiu ; Ren, Baizheng ; Zuo, Zhaohui ; Zhang, Qingyan ; Li, Wenbin ; Song, Bo</creator><creatorcontrib>Wang, Zhikun ; Wang, Yuanzhuo ; Shang, Ping ; Yang, Chao ; Yang, Mingming ; Huang, Jinxiu ; Ren, Baizheng ; Zuo, Zhaohui ; Zhang, Qingyan ; Li, Wenbin ; Song, Bo</creatorcontrib><description>WRINKLED1 (WRI1), an APETALA2/ethylene-responsive-element-binding protein (AP2/EREBP) subfamily transcription factor, plays a crucial role in the transcriptional regulation of plant fatty acid biosynthesis. In this study,
was overexpressed in the soybean cultivar 'Dongnong 50' using
-mediated transformation to generate three transgenic lines with high seed oil contents. PCR and Southern blotting analysis showed that the T-DNA was inserted into the genome at precise insertion sites and was stably inherited by the progeny. Expression analysis using qRT-PCR and Western blotting indicated that
and
driven by the CaMV
promoter were significantly upregulated in the transgenic plants at different developmental stages. Transcriptome sequencing results showed there were obvious differences in gene expression between transgenic line and transgenic receptor during seed developmental stages. KEGG analysis found that the differentially expressed genes mainly annotated to metabolic pathways, such as carbohydrated metabolism and lipid metabolism. A 2-year single-location field trial revealed that three transgenic lines overexpressing
(GmWRI1a-OE) showed a stable increase in seed oil content of 4.97-10.35%. Importantly, no significant effect on protein content and yield was observed. Overexpression of
changed the fatty acid composition by increasing the linoleic acid (C18:2) content and decreasing the palmitic acid (C16:0) content in the seed. The three GmWRI1a-OE lines showed no significant changes in agronomic traits. The results demonstrated that the three
overexpression lines exhibited consistent increases in seed oil content compared with that of the wild type and did not significantly affect the seed yield and agronomic traits. The genetic engineering of
will be an effective strategy for the improvement of seed oil content and value in soybean.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms23095084</identifier><identifier>PMID: 35563472</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Agronomy ; Biosynthesis ; Chromosomes ; Cultivars ; Enzymes ; Fatty acid composition ; Fatty acids ; Fatty Acids - metabolism ; Gene expression ; Gene Expression Regulation, Plant ; Gene regulation ; Genetic engineering ; Genetic transformation ; Genomes ; Germplasm ; Glycine max - genetics ; Glycine max - metabolism ; Insertion ; Linoleic acid ; Lipid metabolism ; Lipids ; Metabolic pathways ; Nutrient content ; Oilseeds ; Palmitic acid ; Plant Oils - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Polymerase chain reaction ; Progeny ; Proteins ; Seeds ; Seeds - metabolism ; Southern blotting ; Soybean Oil - genetics ; Soybean Oil - metabolism ; Soybeans ; T-DNA ; Transcriptomes ; Transgenic plants ; Vegetable oils ; Western blotting</subject><ispartof>International journal of molecular sciences, 2022-05, Vol.23 (9), p.5084</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-e8bfd82b0d82373a0a8017c030a73f4ba79f518ff7fd1bc3957f0e626f44eb983</citedby><cites>FETCH-LOGICAL-c412t-e8bfd82b0d82373a0a8017c030a73f4ba79f518ff7fd1bc3957f0e626f44eb983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102168/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102168/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35563472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Zhikun</creatorcontrib><creatorcontrib>Wang, Yuanzhuo</creatorcontrib><creatorcontrib>Shang, Ping</creatorcontrib><creatorcontrib>Yang, Chao</creatorcontrib><creatorcontrib>Yang, Mingming</creatorcontrib><creatorcontrib>Huang, Jinxiu</creatorcontrib><creatorcontrib>Ren, Baizheng</creatorcontrib><creatorcontrib>Zuo, Zhaohui</creatorcontrib><creatorcontrib>Zhang, Qingyan</creatorcontrib><creatorcontrib>Li, Wenbin</creatorcontrib><creatorcontrib>Song, Bo</creatorcontrib><title>Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>WRINKLED1 (WRI1), an APETALA2/ethylene-responsive-element-binding protein (AP2/EREBP) subfamily transcription factor, plays a crucial role in the transcriptional regulation of plant fatty acid biosynthesis. In this study,
was overexpressed in the soybean cultivar 'Dongnong 50' using
-mediated transformation to generate three transgenic lines with high seed oil contents. PCR and Southern blotting analysis showed that the T-DNA was inserted into the genome at precise insertion sites and was stably inherited by the progeny. Expression analysis using qRT-PCR and Western blotting indicated that
and
driven by the CaMV
promoter were significantly upregulated in the transgenic plants at different developmental stages. Transcriptome sequencing results showed there were obvious differences in gene expression between transgenic line and transgenic receptor during seed developmental stages. KEGG analysis found that the differentially expressed genes mainly annotated to metabolic pathways, such as carbohydrated metabolism and lipid metabolism. A 2-year single-location field trial revealed that three transgenic lines overexpressing
(GmWRI1a-OE) showed a stable increase in seed oil content of 4.97-10.35%. Importantly, no significant effect on protein content and yield was observed. Overexpression of
changed the fatty acid composition by increasing the linoleic acid (C18:2) content and decreasing the palmitic acid (C16:0) content in the seed. The three GmWRI1a-OE lines showed no significant changes in agronomic traits. The results demonstrated that the three
overexpression lines exhibited consistent increases in seed oil content compared with that of the wild type and did not significantly affect the seed yield and agronomic traits. The genetic engineering of
will be an effective strategy for the improvement of seed oil content and value in soybean.</description><subject>Agronomy</subject><subject>Biosynthesis</subject><subject>Chromosomes</subject><subject>Cultivars</subject><subject>Enzymes</subject><subject>Fatty acid composition</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Genetic engineering</subject><subject>Genetic transformation</subject><subject>Genomes</subject><subject>Germplasm</subject><subject>Glycine max - genetics</subject><subject>Glycine max - metabolism</subject><subject>Insertion</subject><subject>Linoleic acid</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Metabolic pathways</subject><subject>Nutrient content</subject><subject>Oilseeds</subject><subject>Palmitic acid</subject><subject>Plant Oils - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Progeny</subject><subject>Proteins</subject><subject>Seeds</subject><subject>Seeds - metabolism</subject><subject>Southern blotting</subject><subject>Soybean Oil - genetics</subject><subject>Soybean Oil - metabolism</subject><subject>Soybeans</subject><subject>T-DNA</subject><subject>Transcriptomes</subject><subject>Transgenic plants</subject><subject>Vegetable oils</subject><subject>Western blotting</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkctLAzEQh4Motj5uniXgxYPVSbK72b0IUnwUCoVW8Riy24lu2U1qshX737ulD6qXyUC--ZjhR8gFg1shMrgrZ3XgArIY0uiAdFnEeQ8gkYd7fYechDAD4ILH2THpiDhORCR5l4xH3-jxZ-4xhNJZ6gyduGWO2tLn-n08YJpOGp1XSzqwhUcdMNDmE-kEcUpHZUX7zjZoG1ra7eAZOTK6Cni-eU_J29Pja_-lNxw9D_oPw14RMd70MM3NNOU5tEVIoUGnwGQBArQUJsq1zEzMUmOkmbK8EFksDWDCExNFmGepOCX3a-98kdc4LdotvK7U3Je19kvldKn-_tjyU324b5Ux4CxZCa43Au--FhgaVZehwKrSFt0iKJ4kkUzbwlr06h86cwtv2_NWlIC0Na6EN2uq8C4Ej2a3DAO1Ckvth9Xil_sH7OBtOuIXe1CP4w</recordid><startdate>20220503</startdate><enddate>20220503</enddate><creator>Wang, Zhikun</creator><creator>Wang, Yuanzhuo</creator><creator>Shang, Ping</creator><creator>Yang, Chao</creator><creator>Yang, Mingming</creator><creator>Huang, Jinxiu</creator><creator>Ren, Baizheng</creator><creator>Zuo, Zhaohui</creator><creator>Zhang, Qingyan</creator><creator>Li, Wenbin</creator><creator>Song, Bo</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220503</creationdate><title>Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean</title><author>Wang, Zhikun ; Wang, Yuanzhuo ; Shang, Ping ; Yang, Chao ; Yang, Mingming ; Huang, Jinxiu ; Ren, Baizheng ; Zuo, Zhaohui ; Zhang, Qingyan ; Li, Wenbin ; Song, Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-e8bfd82b0d82373a0a8017c030a73f4ba79f518ff7fd1bc3957f0e626f44eb983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agronomy</topic><topic>Biosynthesis</topic><topic>Chromosomes</topic><topic>Cultivars</topic><topic>Enzymes</topic><topic>Fatty acid composition</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene regulation</topic><topic>Genetic engineering</topic><topic>Genetic transformation</topic><topic>Genomes</topic><topic>Germplasm</topic><topic>Glycine max - genetics</topic><topic>Glycine max - metabolism</topic><topic>Insertion</topic><topic>Linoleic acid</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Metabolic pathways</topic><topic>Nutrient content</topic><topic>Oilseeds</topic><topic>Palmitic acid</topic><topic>Plant Oils - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Polymerase chain reaction</topic><topic>Progeny</topic><topic>Proteins</topic><topic>Seeds</topic><topic>Seeds - metabolism</topic><topic>Southern blotting</topic><topic>Soybean Oil - genetics</topic><topic>Soybean Oil - metabolism</topic><topic>Soybeans</topic><topic>T-DNA</topic><topic>Transcriptomes</topic><topic>Transgenic plants</topic><topic>Vegetable oils</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zhikun</creatorcontrib><creatorcontrib>Wang, Yuanzhuo</creatorcontrib><creatorcontrib>Shang, Ping</creatorcontrib><creatorcontrib>Yang, Chao</creatorcontrib><creatorcontrib>Yang, Mingming</creatorcontrib><creatorcontrib>Huang, Jinxiu</creatorcontrib><creatorcontrib>Ren, Baizheng</creatorcontrib><creatorcontrib>Zuo, Zhaohui</creatorcontrib><creatorcontrib>Zhang, Qingyan</creatorcontrib><creatorcontrib>Li, Wenbin</creatorcontrib><creatorcontrib>Song, Bo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhikun</au><au>Wang, Yuanzhuo</au><au>Shang, Ping</au><au>Yang, Chao</au><au>Yang, Mingming</au><au>Huang, Jinxiu</au><au>Ren, Baizheng</au><au>Zuo, Zhaohui</au><au>Zhang, Qingyan</au><au>Li, Wenbin</au><au>Song, Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2022-05-03</date><risdate>2022</risdate><volume>23</volume><issue>9</issue><spage>5084</spage><pages>5084-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>WRINKLED1 (WRI1), an APETALA2/ethylene-responsive-element-binding protein (AP2/EREBP) subfamily transcription factor, plays a crucial role in the transcriptional regulation of plant fatty acid biosynthesis. In this study,
was overexpressed in the soybean cultivar 'Dongnong 50' using
-mediated transformation to generate three transgenic lines with high seed oil contents. PCR and Southern blotting analysis showed that the T-DNA was inserted into the genome at precise insertion sites and was stably inherited by the progeny. Expression analysis using qRT-PCR and Western blotting indicated that
and
driven by the CaMV
promoter were significantly upregulated in the transgenic plants at different developmental stages. Transcriptome sequencing results showed there were obvious differences in gene expression between transgenic line and transgenic receptor during seed developmental stages. KEGG analysis found that the differentially expressed genes mainly annotated to metabolic pathways, such as carbohydrated metabolism and lipid metabolism. A 2-year single-location field trial revealed that three transgenic lines overexpressing
(GmWRI1a-OE) showed a stable increase in seed oil content of 4.97-10.35%. Importantly, no significant effect on protein content and yield was observed. Overexpression of
changed the fatty acid composition by increasing the linoleic acid (C18:2) content and decreasing the palmitic acid (C16:0) content in the seed. The three GmWRI1a-OE lines showed no significant changes in agronomic traits. The results demonstrated that the three
overexpression lines exhibited consistent increases in seed oil content compared with that of the wild type and did not significantly affect the seed yield and agronomic traits. The genetic engineering of
will be an effective strategy for the improvement of seed oil content and value in soybean.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35563472</pmid><doi>10.3390/ijms23095084</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Agronomy Biosynthesis Chromosomes Cultivars Enzymes Fatty acid composition Fatty acids Fatty Acids - metabolism Gene expression Gene Expression Regulation, Plant Gene regulation Genetic engineering Genetic transformation Genomes Germplasm Glycine max - genetics Glycine max - metabolism Insertion Linoleic acid Lipid metabolism Lipids Metabolic pathways Nutrient content Oilseeds Palmitic acid Plant Oils - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Polymerase chain reaction Progeny Proteins Seeds Seeds - metabolism Southern blotting Soybean Oil - genetics Soybean Oil - metabolism Soybeans T-DNA Transcriptomes Transgenic plants Vegetable oils Western blotting |
| title | Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean |
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