Whole-body and muscle responses to aerobic exercise training and withdrawal in ageing and COPD
Chronic obstructive pulmonary disease (COPD) patients exhibit lower peak oxygen uptake ( ' ), altered muscle metabolism and impaired exercise tolerance compared with age-matched controls. Whether these traits reflect muscle-level deconditioning (impacted by ventilatory constraints) and/or dysfu...
Gespeichert in:
| Veröffentlicht in: | The European respiratory journal 2022-05, Vol.59 (5), p.2101507 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 5 |
| container_start_page | 2101507 |
| container_title | The European respiratory journal |
| container_volume | 59 |
| creator | Latimer, Lorna E Constantin-Teodosiu, Dumitru Popat, Bhavesh Constantin, Despina Houchen-Wolloff, Linzy Bolton, Charlotte E Steiner, Michael C Greenhaff, Paul L |
| description | Chronic obstructive pulmonary disease (COPD) patients exhibit lower peak oxygen uptake (
'
), altered muscle metabolism and impaired exercise tolerance compared with age-matched controls. Whether these traits reflect muscle-level deconditioning (impacted by ventilatory constraints) and/or dysfunction in mitochondrial ATP production capacity is debated. By studying aerobic exercise training (AET) at a matched relative intensity and subsequent exercise withdrawal period we aimed to elucidate the whole-body and muscle mitochondrial responsiveness of healthy young (HY), healthy older (HO) and COPD volunteers to whole-body exercise.
HY (n=10), HO (n=10) and COPD (n=20) volunteers were studied before and after 8 weeks of AET (65%
'
) and after 4 weeks of exercise withdrawal.
'
, muscle maximal mitochondrial ATP production rate (MAPR), mitochondrial content, mitochondrial DNA (mtDNA) copy number and abundance of 59 targeted fuel metabolism mRNAs were determined at all time-points.
Muscle MAPR (normalised for mitochondrial content) was not different for any substrate combination in HO, HY and COPD at baseline, but mtDNA copy number relative to a nuclear-encoded housekeeping gene (mean±sd) was greater in HY (804±67) than in HO (631±69; p=0.041). AET increased
'
in HO (17%; p=0.002) and HY (21%; p |
| doi_str_mv | 10.1183/13993003.01507-2021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9095946</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2578152510</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-187bd1b372549e455dc6e0dab8d7a6d1f436cecbb7cbf229ee5c0918ee50f6893</originalsourceid><addsrcrecordid>eNpVkc1P3DAQxa2qqCy0fwFS5WMvgZk4TuJLJbSUDwkJDq16q-WPya5RNl7sbIH_niywqD2NNG_emyf9GDtCOEZsxQkKpQSAOAaU0BQllPiBzbbbYrv-yGagQBSoRL3PDnK-A8C6EviJ7YtKti2qesb-_F7Gngob_RM3g-erTXY98UR5HYdMmY-RG0rRBsfpkZILmfiYTBjCsHhxPIRx6ZN5MD0PAzcL2gnzm9uzz2yvM32mL2_zkP06__Fzfllc31xczU-vC1eBHAtsG-vRiqaUlaJKSu9qAm9s6xtTe-wqUTty1jbOdmWpiKQDhe00oatbJQ7Z99fc9cauyDsapo69XqewMulJRxP0_8oQlnoR_2oFSqqqngK-vQWkeL-hPOpVyI763gwUN1mXsmlRlhJhOhWvpy7FnBN1728Q9JaM3pHRL2T0lszk-vpvw3fPDoV4BuNKixw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2578152510</pqid></control><display><type>article</type><title>Whole-body and muscle responses to aerobic exercise training and withdrawal in ageing and COPD</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Latimer, Lorna E ; Constantin-Teodosiu, Dumitru ; Popat, Bhavesh ; Constantin, Despina ; Houchen-Wolloff, Linzy ; Bolton, Charlotte E ; Steiner, Michael C ; Greenhaff, Paul L</creator><creatorcontrib>Latimer, Lorna E ; Constantin-Teodosiu, Dumitru ; Popat, Bhavesh ; Constantin, Despina ; Houchen-Wolloff, Linzy ; Bolton, Charlotte E ; Steiner, Michael C ; Greenhaff, Paul L</creatorcontrib><description>Chronic obstructive pulmonary disease (COPD) patients exhibit lower peak oxygen uptake (
'
), altered muscle metabolism and impaired exercise tolerance compared with age-matched controls. Whether these traits reflect muscle-level deconditioning (impacted by ventilatory constraints) and/or dysfunction in mitochondrial ATP production capacity is debated. By studying aerobic exercise training (AET) at a matched relative intensity and subsequent exercise withdrawal period we aimed to elucidate the whole-body and muscle mitochondrial responsiveness of healthy young (HY), healthy older (HO) and COPD volunteers to whole-body exercise.
HY (n=10), HO (n=10) and COPD (n=20) volunteers were studied before and after 8 weeks of AET (65%
'
) and after 4 weeks of exercise withdrawal.
'
, muscle maximal mitochondrial ATP production rate (MAPR), mitochondrial content, mitochondrial DNA (mtDNA) copy number and abundance of 59 targeted fuel metabolism mRNAs were determined at all time-points.
Muscle MAPR (normalised for mitochondrial content) was not different for any substrate combination in HO, HY and COPD at baseline, but mtDNA copy number relative to a nuclear-encoded housekeeping gene (mean±sd) was greater in HY (804±67) than in HO (631±69; p=0.041). AET increased
'
in HO (17%; p=0.002) and HY (21%; p<0.001), but not COPD (p=0.603). Muscle MAPR for palmitate increased with training in HO (57%; p=0.041) and HY (56%; p=0.003), and decreased with exercise withdrawal in HO (-45%; p=0.036) and HY (-30%; p=0.016), but was unchanged in COPD (p=0.594). mtDNA copy number increased with AET in HY (66%; p=0.001), but not HO (p=0.081) or COPD (p=0.132). The observed changes in muscle mRNA abundance were similar in all groups after AET and exercise withdrawal.
Intrinsic mitochondrial function was not impaired by ageing or COPD in the untrained state. Whole-body and muscle mitochondrial responses to AET were robust in HY, evident in HO, but deficient in COPD. All groups showed robust muscle mRNA responses. Higher relative exercise intensities during whole-body training may be needed to maximise whole-body and muscle mitochondrial adaptation in COPD.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1183/13993003.01507-2021</identifier><identifier>PMID: 34588196</identifier><language>eng</language><publisher>England: European Respiratory Society</publisher><subject>Adenosine Triphosphate - metabolism ; Aging ; DNA, Mitochondrial ; Exercise - physiology ; Exercise Test ; Exercise Tolerance - physiology ; Humans ; Muscles ; Original s ; Oxygen Consumption - physiology ; Pulmonary Disease, Chronic Obstructive ; RNA, Messenger - metabolism</subject><ispartof>The European respiratory journal, 2022-05, Vol.59 (5), p.2101507</ispartof><rights>Copyright ©The authors 2022.</rights><rights>Copyright ©The authors 2022. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-187bd1b372549e455dc6e0dab8d7a6d1f436cecbb7cbf229ee5c0918ee50f6893</citedby><cites>FETCH-LOGICAL-c405t-187bd1b372549e455dc6e0dab8d7a6d1f436cecbb7cbf229ee5c0918ee50f6893</cites><orcidid>0000-0002-9578-2249</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34588196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Latimer, Lorna E</creatorcontrib><creatorcontrib>Constantin-Teodosiu, Dumitru</creatorcontrib><creatorcontrib>Popat, Bhavesh</creatorcontrib><creatorcontrib>Constantin, Despina</creatorcontrib><creatorcontrib>Houchen-Wolloff, Linzy</creatorcontrib><creatorcontrib>Bolton, Charlotte E</creatorcontrib><creatorcontrib>Steiner, Michael C</creatorcontrib><creatorcontrib>Greenhaff, Paul L</creatorcontrib><title>Whole-body and muscle responses to aerobic exercise training and withdrawal in ageing and COPD</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>Chronic obstructive pulmonary disease (COPD) patients exhibit lower peak oxygen uptake (
'
), altered muscle metabolism and impaired exercise tolerance compared with age-matched controls. Whether these traits reflect muscle-level deconditioning (impacted by ventilatory constraints) and/or dysfunction in mitochondrial ATP production capacity is debated. By studying aerobic exercise training (AET) at a matched relative intensity and subsequent exercise withdrawal period we aimed to elucidate the whole-body and muscle mitochondrial responsiveness of healthy young (HY), healthy older (HO) and COPD volunteers to whole-body exercise.
HY (n=10), HO (n=10) and COPD (n=20) volunteers were studied before and after 8 weeks of AET (65%
'
) and after 4 weeks of exercise withdrawal.
'
, muscle maximal mitochondrial ATP production rate (MAPR), mitochondrial content, mitochondrial DNA (mtDNA) copy number and abundance of 59 targeted fuel metabolism mRNAs were determined at all time-points.
Muscle MAPR (normalised for mitochondrial content) was not different for any substrate combination in HO, HY and COPD at baseline, but mtDNA copy number relative to a nuclear-encoded housekeeping gene (mean±sd) was greater in HY (804±67) than in HO (631±69; p=0.041). AET increased
'
in HO (17%; p=0.002) and HY (21%; p<0.001), but not COPD (p=0.603). Muscle MAPR for palmitate increased with training in HO (57%; p=0.041) and HY (56%; p=0.003), and decreased with exercise withdrawal in HO (-45%; p=0.036) and HY (-30%; p=0.016), but was unchanged in COPD (p=0.594). mtDNA copy number increased with AET in HY (66%; p=0.001), but not HO (p=0.081) or COPD (p=0.132). The observed changes in muscle mRNA abundance were similar in all groups after AET and exercise withdrawal.
Intrinsic mitochondrial function was not impaired by ageing or COPD in the untrained state. Whole-body and muscle mitochondrial responses to AET were robust in HY, evident in HO, but deficient in COPD. All groups showed robust muscle mRNA responses. Higher relative exercise intensities during whole-body training may be needed to maximise whole-body and muscle mitochondrial adaptation in COPD.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Aging</subject><subject>DNA, Mitochondrial</subject><subject>Exercise - physiology</subject><subject>Exercise Test</subject><subject>Exercise Tolerance - physiology</subject><subject>Humans</subject><subject>Muscles</subject><subject>Original s</subject><subject>Oxygen Consumption - physiology</subject><subject>Pulmonary Disease, Chronic Obstructive</subject><subject>RNA, Messenger - metabolism</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1P3DAQxa2qqCy0fwFS5WMvgZk4TuJLJbSUDwkJDq16q-WPya5RNl7sbIH_niywqD2NNG_emyf9GDtCOEZsxQkKpQSAOAaU0BQllPiBzbbbYrv-yGagQBSoRL3PDnK-A8C6EviJ7YtKti2qesb-_F7Gngob_RM3g-erTXY98UR5HYdMmY-RG0rRBsfpkZILmfiYTBjCsHhxPIRx6ZN5MD0PAzcL2gnzm9uzz2yvM32mL2_zkP06__Fzfllc31xczU-vC1eBHAtsG-vRiqaUlaJKSu9qAm9s6xtTe-wqUTty1jbOdmWpiKQDhe00oatbJQ7Z99fc9cauyDsapo69XqewMulJRxP0_8oQlnoR_2oFSqqqngK-vQWkeL-hPOpVyI763gwUN1mXsmlRlhJhOhWvpy7FnBN1728Q9JaM3pHRL2T0lszk-vpvw3fPDoV4BuNKixw</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Latimer, Lorna E</creator><creator>Constantin-Teodosiu, Dumitru</creator><creator>Popat, Bhavesh</creator><creator>Constantin, Despina</creator><creator>Houchen-Wolloff, Linzy</creator><creator>Bolton, Charlotte E</creator><creator>Steiner, Michael C</creator><creator>Greenhaff, Paul L</creator><general>European Respiratory Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9578-2249</orcidid></search><sort><creationdate>20220501</creationdate><title>Whole-body and muscle responses to aerobic exercise training and withdrawal in ageing and COPD</title><author>Latimer, Lorna E ; Constantin-Teodosiu, Dumitru ; Popat, Bhavesh ; Constantin, Despina ; Houchen-Wolloff, Linzy ; Bolton, Charlotte E ; Steiner, Michael C ; Greenhaff, Paul L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-187bd1b372549e455dc6e0dab8d7a6d1f436cecbb7cbf229ee5c0918ee50f6893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Aging</topic><topic>DNA, Mitochondrial</topic><topic>Exercise - physiology</topic><topic>Exercise Test</topic><topic>Exercise Tolerance - physiology</topic><topic>Humans</topic><topic>Muscles</topic><topic>Original s</topic><topic>Oxygen Consumption - physiology</topic><topic>Pulmonary Disease, Chronic Obstructive</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Latimer, Lorna E</creatorcontrib><creatorcontrib>Constantin-Teodosiu, Dumitru</creatorcontrib><creatorcontrib>Popat, Bhavesh</creatorcontrib><creatorcontrib>Constantin, Despina</creatorcontrib><creatorcontrib>Houchen-Wolloff, Linzy</creatorcontrib><creatorcontrib>Bolton, Charlotte E</creatorcontrib><creatorcontrib>Steiner, Michael C</creatorcontrib><creatorcontrib>Greenhaff, Paul L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Latimer, Lorna E</au><au>Constantin-Teodosiu, Dumitru</au><au>Popat, Bhavesh</au><au>Constantin, Despina</au><au>Houchen-Wolloff, Linzy</au><au>Bolton, Charlotte E</au><au>Steiner, Michael C</au><au>Greenhaff, Paul L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole-body and muscle responses to aerobic exercise training and withdrawal in ageing and COPD</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>59</volume><issue>5</issue><spage>2101507</spage><pages>2101507-</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>Chronic obstructive pulmonary disease (COPD) patients exhibit lower peak oxygen uptake (
'
), altered muscle metabolism and impaired exercise tolerance compared with age-matched controls. Whether these traits reflect muscle-level deconditioning (impacted by ventilatory constraints) and/or dysfunction in mitochondrial ATP production capacity is debated. By studying aerobic exercise training (AET) at a matched relative intensity and subsequent exercise withdrawal period we aimed to elucidate the whole-body and muscle mitochondrial responsiveness of healthy young (HY), healthy older (HO) and COPD volunteers to whole-body exercise.
HY (n=10), HO (n=10) and COPD (n=20) volunteers were studied before and after 8 weeks of AET (65%
'
) and after 4 weeks of exercise withdrawal.
'
, muscle maximal mitochondrial ATP production rate (MAPR), mitochondrial content, mitochondrial DNA (mtDNA) copy number and abundance of 59 targeted fuel metabolism mRNAs were determined at all time-points.
Muscle MAPR (normalised for mitochondrial content) was not different for any substrate combination in HO, HY and COPD at baseline, but mtDNA copy number relative to a nuclear-encoded housekeeping gene (mean±sd) was greater in HY (804±67) than in HO (631±69; p=0.041). AET increased
'
in HO (17%; p=0.002) and HY (21%; p<0.001), but not COPD (p=0.603). Muscle MAPR for palmitate increased with training in HO (57%; p=0.041) and HY (56%; p=0.003), and decreased with exercise withdrawal in HO (-45%; p=0.036) and HY (-30%; p=0.016), but was unchanged in COPD (p=0.594). mtDNA copy number increased with AET in HY (66%; p=0.001), but not HO (p=0.081) or COPD (p=0.132). The observed changes in muscle mRNA abundance were similar in all groups after AET and exercise withdrawal.
Intrinsic mitochondrial function was not impaired by ageing or COPD in the untrained state. Whole-body and muscle mitochondrial responses to AET were robust in HY, evident in HO, but deficient in COPD. All groups showed robust muscle mRNA responses. Higher relative exercise intensities during whole-body training may be needed to maximise whole-body and muscle mitochondrial adaptation in COPD.</abstract><cop>England</cop><pub>European Respiratory Society</pub><pmid>34588196</pmid><doi>10.1183/13993003.01507-2021</doi><orcidid>https://orcid.org/0000-0002-9578-2249</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0903-1936 |
| ispartof | The European respiratory journal, 2022-05, Vol.59 (5), p.2101507 |
| issn | 0903-1936 1399-3003 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9095946 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenosine Triphosphate - metabolism Aging DNA, Mitochondrial Exercise - physiology Exercise Test Exercise Tolerance - physiology Humans Muscles Original s Oxygen Consumption - physiology Pulmonary Disease, Chronic Obstructive RNA, Messenger - metabolism |
| title | Whole-body and muscle responses to aerobic exercise training and withdrawal in ageing and COPD |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T18%3A33%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Whole-body%20and%20muscle%20responses%20to%20aerobic%20exercise%20training%20and%20withdrawal%20in%20ageing%20and%20COPD&rft.jtitle=The%20European%20respiratory%20journal&rft.au=Latimer,%20Lorna%20E&rft.date=2022-05-01&rft.volume=59&rft.issue=5&rft.spage=2101507&rft.pages=2101507-&rft.issn=0903-1936&rft.eissn=1399-3003&rft_id=info:doi/10.1183/13993003.01507-2021&rft_dat=%3Cproquest_pubme%3E2578152510%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2578152510&rft_id=info:pmid/34588196&rfr_iscdi=true |