Molecular cloning and functional expression in Lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis

A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acid...

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Veröffentlicht in:	Applied and environmental microbiology 1998-12, Vol.64 (12), p.4720-4728
Hauptverfasser:	Chaillou, S. (University of Amsterdam, Amsterdam.), Bor, Y.C, Batt, C.A, Postma, P.W, Pouwels, P.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacteria Bacterial Proteins Bacteriology Base Sequence Biological and medical sciences Biotechnology Carrier Proteins - genetics Carrier Proteins - metabolism Cloning Cloning, Molecular EXPRESION GENICA EXPRESSION DES GENES Fermentation Food Microbiology Fundamental and applied biological sciences. Psychology GENBANK/AF04552 GENE EXPRESSION GENE TRANSFER Genes Genetic engineering Genetic technics GENETIC TRANSFORMATION Kinetics Lactobacillus - genetics Lactobacillus - metabolism Life Sciences Methods. Procedures. Technologies Microbiology and Parasitology Molecular cloning MOLECULAR SEQUENCE DATA NUCLEOTIDE SEQUENCE Open Reading Frames Polymerase Chain Reaction Recombinant Proteins - metabolism Restriction Mapping SECUENCIA NUCLEOTIDICA Sequence Alignment Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE Substrate Specificity Symporters TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Xylose - metabolism
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