Analysis of mitochondrial DNA cytochrome‐b (CYB) and ATPase‐6 gene mutations in COVID‐19 patients
Coronavirus disease of 2019 (COVID‐19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome‐b (CYB) and adenosine t...
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| Veröffentlicht in: | Journal of medical virology 2022-07, Vol.94 (7), p.3138-3146 |
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| description | Coronavirus disease of 2019 (COVID‐19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome‐b (CYB) and adenosine triphosphatase‐6 (ATPase‐6) genes of mtDNA in COVID‐19 patients. The association between mtDNA mutations and clinical outcomes is investigated here. In the present study, mutations of the mtDNA genes CYB and ATPase‐6 were investigated in COVID‐19 (+) (n = 65) and COVID‐19 (−) patients (n = 65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing. The age, creatinine, ferritin, and CRP levels of the COVID 19 (+) patients were higher than those of the COVID‐19 (−) patients (p = 0.0036, p = 0.0383, p = 0.0305, p |
| doi_str_mv | 10.1002/jmv.27704 |
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Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome‐b (CYB) and adenosine triphosphatase‐6 (ATPase‐6) genes of mtDNA in COVID‐19 patients. The association between mtDNA mutations and clinical outcomes is investigated here. In the present study, mutations of the mtDNA genes CYB and ATPase‐6 were investigated in COVID‐19 (+) (n = 65) and COVID‐19 (−) patients (n = 65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing. The age, creatinine, ferritin, and CRP levels of the COVID 19 (+) patients were higher than those of the COVID‐19 (−) patients (p = 0.0036, p = 0.0383, p = 0.0305, p < 0.0001, respectively). We also found 16 different mutations in the CYB gene and 14 different mutations in the ATPase‐6 gene. The incidences of CYB gene mutations A15326G, T15454C, and C15452A were higher in COVID‐19 (+) patients than COVID‐19 (−) patients; p < 0.0001: OR (95% CI): 4.966 (2.215−10.89), p = 0.0226, and p = 0.0226, respectively. In contrast, the incidences of A8860G and G9055A ATPase‐6 gene mutations were higher in COVID‐19 (+) patients than COVID‐19 (−) patients; p < 0.0001: OR (95%CI): 5.333 (2.359−12.16) and p = 0.0121 respectively. Yet, no significant relationship was found between mtDNA mutations and patients' age and biochemical parameters (p > 0.05). The results showed that the frequency of mtDNA mutations in COVID‐19 patients is quite high and it is important to investigate the association of these mutations with other genetic mechanisms in larger patient populations.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.27704</identifier><identifier>PMID: 35258110</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adenosine ; Adenosine triphosphatase ; ATPase‐6 ; Coronaviruses ; COVID-19 ; Creatinine ; CYB ; Cytochrome ; Cytochromes ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; Ferritin ; Genes ; Mitochondrial DNA ; mtDNA ; Mutation ; Pandemics ; Patients ; PCR ; Sanger sequencing ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Viral diseases ; Virology</subject><ispartof>Journal of medical virology, 2022-07, Vol.94 (7), p.3138-3146</ispartof><rights>2022 Wiley Periodicals LLC</rights><rights>2022 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4434-9a23de8a74883688ec664326ff179388033423f3336e7f21793566308dedd2c23</citedby><cites>FETCH-LOGICAL-c4434-9a23de8a74883688ec664326ff179388033423f3336e7f21793566308dedd2c23</cites><orcidid>0000-0003-3552-5459 ; 0000-0001-5201-6352 ; 0000-0002-6512-2987 ; 0000-0001-9260-5223 ; 0000-0001-7027-1613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.27704$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.27704$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35258110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dirican, Ebubekir</creatorcontrib><creatorcontrib>Savrun, Şeyda Tuba</creatorcontrib><creatorcontrib>Aydın, İsmail Erkan</creatorcontrib><creatorcontrib>Gülbay, Gonca</creatorcontrib><creatorcontrib>Karaman, Ülkü</creatorcontrib><title>Analysis of mitochondrial DNA cytochrome‐b (CYB) and ATPase‐6 gene mutations in COVID‐19 patients</title><title>Journal of medical virology</title><addtitle>J Med Virol</addtitle><description>Coronavirus disease of 2019 (COVID‐19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome‐b (CYB) and adenosine triphosphatase‐6 (ATPase‐6) genes of mtDNA in COVID‐19 patients. The association between mtDNA mutations and clinical outcomes is investigated here. In the present study, mutations of the mtDNA genes CYB and ATPase‐6 were investigated in COVID‐19 (+) (n = 65) and COVID‐19 (−) patients (n = 65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing. The age, creatinine, ferritin, and CRP levels of the COVID 19 (+) patients were higher than those of the COVID‐19 (−) patients (p = 0.0036, p = 0.0383, p = 0.0305, p < 0.0001, respectively). We also found 16 different mutations in the CYB gene and 14 different mutations in the ATPase‐6 gene. The incidences of CYB gene mutations A15326G, T15454C, and C15452A were higher in COVID‐19 (+) patients than COVID‐19 (−) patients; p < 0.0001: OR (95% CI): 4.966 (2.215−10.89), p = 0.0226, and p = 0.0226, respectively. In contrast, the incidences of A8860G and G9055A ATPase‐6 gene mutations were higher in COVID‐19 (+) patients than COVID‐19 (−) patients; p < 0.0001: OR (95%CI): 5.333 (2.359−12.16) and p = 0.0121 respectively. Yet, no significant relationship was found between mtDNA mutations and patients' age and biochemical parameters (p > 0.05). The results showed that the frequency of mtDNA mutations in COVID‐19 patients is quite high and it is important to investigate the association of these mutations with other genetic mechanisms in larger patient populations.</description><subject>Adenosine</subject><subject>Adenosine triphosphatase</subject><subject>ATPase‐6</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Creatinine</subject><subject>CYB</subject><subject>Cytochrome</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>Ferritin</subject><subject>Genes</subject><subject>Mitochondrial DNA</subject><subject>mtDNA</subject><subject>Mutation</subject><subject>Pandemics</subject><subject>Patients</subject><subject>PCR</subject><subject>Sanger sequencing</subject><subject>Severe acute respiratory syndrome</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Viral diseases</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kcFu1DAURS1ERYfCgh9Alti0i7S2n2M7G6RhCrSoUBalEivLTZypR4k92EnR7PgEvpEvqdMpFSCxsnTf0fHTuwi9oOSQEsKOVv3NIZOS8EdoRkkliopI-hjNCOWiEIKWu-hpSitCiKoYe4J2oWSlopTM0HLuTbdJLuHQ4t4Nob4OvonOdPj40xzXmymJobe_fvy8wvuLr28OsPENnl98NmkKBV5ab3E_DmZwwSfsPF6cX54e5xmt8Dqn1g_pGdppTZfs8_t3D3159_ZicVKcnb8_XczPippz4EVlGDRWGcmVAqGUrYXgwETbUlmBUgSAM2gBQFjZsikshQCiGts0rGawh15vvevxqrdNnf-OptPr6HoTNzoYp_-eeHetl-FGV0QpUfEs2L8XxPBttGnQvUu17TrjbRiTZgIkgFQUMvrqH3QVxpjvOVGiLEtFSpmpgy1Vx5BStO3DMpToqT6d69N39WX25Z_bP5C_-8rA0Rb47jq7-b9Jf_h4uVXeAtONpIA</recordid><startdate>202207</startdate><enddate>202207</enddate><creator>Dirican, Ebubekir</creator><creator>Savrun, Şeyda Tuba</creator><creator>Aydın, İsmail Erkan</creator><creator>Gülbay, Gonca</creator><creator>Karaman, Ülkü</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3552-5459</orcidid><orcidid>https://orcid.org/0000-0001-5201-6352</orcidid><orcidid>https://orcid.org/0000-0002-6512-2987</orcidid><orcidid>https://orcid.org/0000-0001-9260-5223</orcidid><orcidid>https://orcid.org/0000-0001-7027-1613</orcidid></search><sort><creationdate>202207</creationdate><title>Analysis of mitochondrial DNA cytochrome‐b (CYB) and ATPase‐6 gene mutations in COVID‐19 patients</title><author>Dirican, Ebubekir ; Savrun, Şeyda Tuba ; Aydın, İsmail Erkan ; Gülbay, Gonca ; Karaman, Ülkü</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4434-9a23de8a74883688ec664326ff179388033423f3336e7f21793566308dedd2c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenosine</topic><topic>Adenosine triphosphatase</topic><topic>ATPase‐6</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>Creatinine</topic><topic>CYB</topic><topic>Cytochrome</topic><topic>Cytochromes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>Ferritin</topic><topic>Genes</topic><topic>Mitochondrial DNA</topic><topic>mtDNA</topic><topic>Mutation</topic><topic>Pandemics</topic><topic>Patients</topic><topic>PCR</topic><topic>Sanger sequencing</topic><topic>Severe acute respiratory syndrome</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Viral diseases</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dirican, Ebubekir</creatorcontrib><creatorcontrib>Savrun, Şeyda Tuba</creatorcontrib><creatorcontrib>Aydın, İsmail Erkan</creatorcontrib><creatorcontrib>Gülbay, Gonca</creatorcontrib><creatorcontrib>Karaman, Ülkü</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dirican, Ebubekir</au><au>Savrun, Şeyda Tuba</au><au>Aydın, İsmail Erkan</au><au>Gülbay, Gonca</au><au>Karaman, Ülkü</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of mitochondrial DNA cytochrome‐b (CYB) and ATPase‐6 gene mutations in COVID‐19 patients</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J Med Virol</addtitle><date>2022-07</date><risdate>2022</risdate><volume>94</volume><issue>7</issue><spage>3138</spage><epage>3146</epage><pages>3138-3146</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><abstract>Coronavirus disease of 2019 (COVID‐19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome‐b (CYB) and adenosine triphosphatase‐6 (ATPase‐6) genes of mtDNA in COVID‐19 patients. The association between mtDNA mutations and clinical outcomes is investigated here. In the present study, mutations of the mtDNA genes CYB and ATPase‐6 were investigated in COVID‐19 (+) (n = 65) and COVID‐19 (−) patients (n = 65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing. The age, creatinine, ferritin, and CRP levels of the COVID 19 (+) patients were higher than those of the COVID‐19 (−) patients (p = 0.0036, p = 0.0383, p = 0.0305, p < 0.0001, respectively). We also found 16 different mutations in the CYB gene and 14 different mutations in the ATPase‐6 gene. The incidences of CYB gene mutations A15326G, T15454C, and C15452A were higher in COVID‐19 (+) patients than COVID‐19 (−) patients; p < 0.0001: OR (95% CI): 4.966 (2.215−10.89), p = 0.0226, and p = 0.0226, respectively. In contrast, the incidences of A8860G and G9055A ATPase‐6 gene mutations were higher in COVID‐19 (+) patients than COVID‐19 (−) patients; p < 0.0001: OR (95%CI): 5.333 (2.359−12.16) and p = 0.0121 respectively. Yet, no significant relationship was found between mtDNA mutations and patients' age and biochemical parameters (p > 0.05). The results showed that the frequency of mtDNA mutations in COVID‐19 patients is quite high and it is important to investigate the association of these mutations with other genetic mechanisms in larger patient populations.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35258110</pmid><doi>10.1002/jmv.27704</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3552-5459</orcidid><orcidid>https://orcid.org/0000-0001-5201-6352</orcidid><orcidid>https://orcid.org/0000-0002-6512-2987</orcidid><orcidid>https://orcid.org/0000-0001-9260-5223</orcidid><orcidid>https://orcid.org/0000-0001-7027-1613</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Adenosine triphosphatase ATPase‐6 Coronaviruses COVID-19 Creatinine CYB Cytochrome Cytochromes Deoxyribonucleic acid DNA DNA sequencing Ferritin Genes Mitochondrial DNA mtDNA Mutation Pandemics Patients PCR Sanger sequencing Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Viral diseases Virology |
| title | Analysis of mitochondrial DNA cytochrome‐b (CYB) and ATPase‐6 gene mutations in COVID‐19 patients |
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