Bee venom protects against pancreatic cancer via inducing cell cycle arrest and apoptosis with suppression of cell migration
Pancreatic cancer seriously threatens human health. Bee venom is a mixture of enzymes, peptides, and amines. Due to its biological activity, bee venom is widely used as an anti-inflammatory agent and pain reliever. However, little is known about the effect of bee venom on pancreatic cancer. Firstly,...
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| Veröffentlicht in: | Journal of gastrointestinal oncology 2022-04, Vol.13 (2), p.847-858 |
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| container_title | Journal of gastrointestinal oncology |
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| creator | Zhao, Jing Hu, Weiguo Zhang, Zejia Zhou, Zegao Duan, Jiayue Dong, Zheng Liu, Hao Yan, Changqing |
| description | Pancreatic cancer seriously threatens human health. Bee venom is a mixture of enzymes, peptides, and amines. Due to its biological activity, bee venom is widely used as an anti-inflammatory agent and pain reliever. However, little is known about the effect of bee venom on pancreatic cancer.
Firstly, the Cell Counting Kit-8 (CCK-8) assay was conducted to analyze the cytotoxicity of bee venom on PANC-1 and AsPC-1 cells. Then, we evaluated the cell cycle and apoptosis by flow cytometry and the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. In addition, cell migration was analyzed by the cell scratch test and Transwell assay. Western blot was performed to assess the expression of proteins involved in the regulation of cell cycle arrest and apoptosis.
Results demonstrated that bee venom significantly suppressed cell proliferation via inducing cell cycle arrest and apoptosis with suppression of cell migration. Bee venom induced S phase arrest and ameliorated the protein expression of cyclins and cyclin-dependent kinases (CDKs). At the same time, bee venom can activate the p53-p21 pathway. Experimental data also showed that bee venom induced cell apoptosis and impeded cell migration.
The present study revealed that bee venom could effectively inhibit tumor progression in pancreatic cancer cells, indicating the possibility of bee venom as an anti-tumor drug in pancreatic cancer. |
| doi_str_mv | 10.21037/jgo-22-222 |
| format | Article |
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Firstly, the Cell Counting Kit-8 (CCK-8) assay was conducted to analyze the cytotoxicity of bee venom on PANC-1 and AsPC-1 cells. Then, we evaluated the cell cycle and apoptosis by flow cytometry and the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. In addition, cell migration was analyzed by the cell scratch test and Transwell assay. Western blot was performed to assess the expression of proteins involved in the regulation of cell cycle arrest and apoptosis.
Results demonstrated that bee venom significantly suppressed cell proliferation via inducing cell cycle arrest and apoptosis with suppression of cell migration. Bee venom induced S phase arrest and ameliorated the protein expression of cyclins and cyclin-dependent kinases (CDKs). At the same time, bee venom can activate the p53-p21 pathway. Experimental data also showed that bee venom induced cell apoptosis and impeded cell migration.
The present study revealed that bee venom could effectively inhibit tumor progression in pancreatic cancer cells, indicating the possibility of bee venom as an anti-tumor drug in pancreatic cancer.</description><identifier>ISSN: 2078-6891</identifier><identifier>EISSN: 2219-679X</identifier><identifier>DOI: 10.21037/jgo-22-222</identifier><identifier>PMID: 35557571</identifier><language>eng</language><publisher>China: AME Publishing Company</publisher><subject>Original</subject><ispartof>Journal of gastrointestinal oncology, 2022-04, Vol.13 (2), p.847-858</ispartof><rights>2022 Journal of Gastrointestinal Oncology. All rights reserved.</rights><rights>2022 Journal of Gastrointestinal Oncology. All rights reserved. 2022 Journal of Gastrointestinal Oncology.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-498b9fd61326b310e840be2c404c1e7b255a71f0bc873286fd34ca99164134373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086033/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086033/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35557571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Jing</creatorcontrib><creatorcontrib>Hu, Weiguo</creatorcontrib><creatorcontrib>Zhang, Zejia</creatorcontrib><creatorcontrib>Zhou, Zegao</creatorcontrib><creatorcontrib>Duan, Jiayue</creatorcontrib><creatorcontrib>Dong, Zheng</creatorcontrib><creatorcontrib>Liu, Hao</creatorcontrib><creatorcontrib>Yan, Changqing</creatorcontrib><title>Bee venom protects against pancreatic cancer via inducing cell cycle arrest and apoptosis with suppression of cell migration</title><title>Journal of gastrointestinal oncology</title><addtitle>J Gastrointest Oncol</addtitle><description>Pancreatic cancer seriously threatens human health. Bee venom is a mixture of enzymes, peptides, and amines. Due to its biological activity, bee venom is widely used as an anti-inflammatory agent and pain reliever. However, little is known about the effect of bee venom on pancreatic cancer.
Firstly, the Cell Counting Kit-8 (CCK-8) assay was conducted to analyze the cytotoxicity of bee venom on PANC-1 and AsPC-1 cells. Then, we evaluated the cell cycle and apoptosis by flow cytometry and the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. In addition, cell migration was analyzed by the cell scratch test and Transwell assay. Western blot was performed to assess the expression of proteins involved in the regulation of cell cycle arrest and apoptosis.
Results demonstrated that bee venom significantly suppressed cell proliferation via inducing cell cycle arrest and apoptosis with suppression of cell migration. Bee venom induced S phase arrest and ameliorated the protein expression of cyclins and cyclin-dependent kinases (CDKs). At the same time, bee venom can activate the p53-p21 pathway. Experimental data also showed that bee venom induced cell apoptosis and impeded cell migration.
The present study revealed that bee venom could effectively inhibit tumor progression in pancreatic cancer cells, indicating the possibility of bee venom as an anti-tumor drug in pancreatic cancer.</description><subject>Original</subject><issn>2078-6891</issn><issn>2219-679X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpVUc1LHzEQDVJRUU-9lxwLZTVfm2wuhVbqBwheFHoL2ezsGtlNtsnuT4T-8UZ_VuowMAPz5r0ZHkKfKTlhlHB1-jDEirGSbAcdMEZ1JZX-_an0RDWVbDTdR8c5P5ASQtekZnton9d1rWpFD9DfnwB4AyFOeE5xAbdkbAfrQ17wbINLYBfvsCstJLzxFvvQrc6HATsYR-ye3AjYpgRlwYYO2znOS8w-40e_3OO8znOZZR8Djv12Z_JDKqwxHKHd3o4Zjt_qIbo7_3V7dlld31xcnf24rhyndKmEblrdd5JyJltOCTSCtMCcIMJRUC2ra6toT1rXKM4a2XdcOKs1lYJywRU_RN-3vPPaTtA5CEuyo5mTn2x6MtF683ES_L0Z4sZo0kjCeSH4-kaQ4p-1vGomn19-sQHimg2TUijNOHnR-raFuhRzTtC_y1BiXi0zxTLDWElW0F_-v-wd-88g_gyMmZSX</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Zhao, Jing</creator><creator>Hu, Weiguo</creator><creator>Zhang, Zejia</creator><creator>Zhou, Zegao</creator><creator>Duan, Jiayue</creator><creator>Dong, Zheng</creator><creator>Liu, Hao</creator><creator>Yan, Changqing</creator><general>AME Publishing Company</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>202204</creationdate><title>Bee venom protects against pancreatic cancer via inducing cell cycle arrest and apoptosis with suppression of cell migration</title><author>Zhao, Jing ; Hu, Weiguo ; Zhang, Zejia ; Zhou, Zegao ; Duan, Jiayue ; Dong, Zheng ; Liu, Hao ; Yan, Changqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-498b9fd61326b310e840be2c404c1e7b255a71f0bc873286fd34ca99164134373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Original</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Jing</creatorcontrib><creatorcontrib>Hu, Weiguo</creatorcontrib><creatorcontrib>Zhang, Zejia</creatorcontrib><creatorcontrib>Zhou, Zegao</creatorcontrib><creatorcontrib>Duan, Jiayue</creatorcontrib><creatorcontrib>Dong, Zheng</creatorcontrib><creatorcontrib>Liu, Hao</creatorcontrib><creatorcontrib>Yan, Changqing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of gastrointestinal oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Jing</au><au>Hu, Weiguo</au><au>Zhang, Zejia</au><au>Zhou, Zegao</au><au>Duan, Jiayue</au><au>Dong, Zheng</au><au>Liu, Hao</au><au>Yan, Changqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bee venom protects against pancreatic cancer via inducing cell cycle arrest and apoptosis with suppression of cell migration</atitle><jtitle>Journal of gastrointestinal oncology</jtitle><addtitle>J Gastrointest Oncol</addtitle><date>2022-04</date><risdate>2022</risdate><volume>13</volume><issue>2</issue><spage>847</spage><epage>858</epage><pages>847-858</pages><issn>2078-6891</issn><eissn>2219-679X</eissn><abstract>Pancreatic cancer seriously threatens human health. Bee venom is a mixture of enzymes, peptides, and amines. Due to its biological activity, bee venom is widely used as an anti-inflammatory agent and pain reliever. However, little is known about the effect of bee venom on pancreatic cancer.
Firstly, the Cell Counting Kit-8 (CCK-8) assay was conducted to analyze the cytotoxicity of bee venom on PANC-1 and AsPC-1 cells. Then, we evaluated the cell cycle and apoptosis by flow cytometry and the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. In addition, cell migration was analyzed by the cell scratch test and Transwell assay. Western blot was performed to assess the expression of proteins involved in the regulation of cell cycle arrest and apoptosis.
Results demonstrated that bee venom significantly suppressed cell proliferation via inducing cell cycle arrest and apoptosis with suppression of cell migration. Bee venom induced S phase arrest and ameliorated the protein expression of cyclins and cyclin-dependent kinases (CDKs). At the same time, bee venom can activate the p53-p21 pathway. Experimental data also showed that bee venom induced cell apoptosis and impeded cell migration.
The present study revealed that bee venom could effectively inhibit tumor progression in pancreatic cancer cells, indicating the possibility of bee venom as an anti-tumor drug in pancreatic cancer.</abstract><cop>China</cop><pub>AME Publishing Company</pub><pmid>35557571</pmid><doi>10.21037/jgo-22-222</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| title | Bee venom protects against pancreatic cancer via inducing cell cycle arrest and apoptosis with suppression of cell migration |
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