Role of Resveratrol as Radiosensitizer by Targeting Cancer Stem Cells in Radioresistant Prostate Cancer Cells (PC-3)
Here, we examined the role of resveratrol as a radiosensitizer by targeting cancer stem cells in radioresistant prostate cancer cells (PC-3) using stem cell markers CD44, CD49b and CD29, SOX2, OCT4, CXCR4, DCLK1 and EMT markers such as VIM and E-cadherin. This study was an in vitro study involving P...
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| Veröffentlicht in: | Asian Pacific Journal of Cancer Prevention 2021-12, Vol.22 (12), p.3823-3837 |
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| container_issue | 12 |
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| container_title | Asian Pacific Journal of Cancer Prevention |
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| creator | El-Benhawy, Sanaa A Morsi, Mohmed I Fahmy, Enayat I Soula, Moustafa A Khalil, Fatma Al Zahraa Fh Arab, Amal Refaat |
| description | Here, we examined the role of resveratrol as a radiosensitizer by targeting cancer stem cells in radioresistant prostate cancer cells (PC-3) using stem cell markers CD44, CD49b and CD29, SOX2, OCT4, CXCR4, DCLK1 and EMT markers such as VIM and E-cadherin.
This study was an in vitro study involving PC-3 cell line which was dividing into four groups. Group I (CO): Control group composed of cells grown in the same medium without treatment with ionizing radiation or resveratrol. Group II (IR): Cells were treated with ionizing radiation alone. Group III (RV): Cells were treated with resveratrol alone. Group VI (IR&RV): The cells were treated with ionizing radiation and resveratrol in combination. The viability of cells was assessed by MTT assay. Genes of interest were measured by RT-PCR and the radiosensitizing efficacy of RV on proliferating cancer cells was determined by clonogenic assay.
Ionizing radiation significantly reduced PC-3 viability, lowered stem cell markers and affected epithelial to mesenchymal transition (EMT) genes expression at all doses (2, 4, 6 and 8 Gray). Resveratrol significantly decreased PC-3 viability and lowered stem cell markers and EMT genes expression at concentrations 35, 70 and 140 µM. Combining resveratrol treatment with ionizing radiation leads to significant reduction in cell viability and stem cell markers genes which was noticed with increasing the radiation dose when compared to ionizing radiation alone treated group.
Resveratrol has a radiosensitizing effect, that ability is triggered by reducing the expression of cancer stem cell markers and affecting EMT markers. Resveratrol showed to be a good candidate for further studies as anticancer drug in the treatment of human prostate cancer. |
| doi_str_mv | 10.31557/APJCP.2021.22.12.3823 |
| format | Article |
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This study was an in vitro study involving PC-3 cell line which was dividing into four groups. Group I (CO): Control group composed of cells grown in the same medium without treatment with ionizing radiation or resveratrol. Group II (IR): Cells were treated with ionizing radiation alone. Group III (RV): Cells were treated with resveratrol alone. Group VI (IR&RV): The cells were treated with ionizing radiation and resveratrol in combination. The viability of cells was assessed by MTT assay. Genes of interest were measured by RT-PCR and the radiosensitizing efficacy of RV on proliferating cancer cells was determined by clonogenic assay.
Ionizing radiation significantly reduced PC-3 viability, lowered stem cell markers and affected epithelial to mesenchymal transition (EMT) genes expression at all doses (2, 4, 6 and 8 Gray). Resveratrol significantly decreased PC-3 viability and lowered stem cell markers and EMT genes expression at concentrations 35, 70 and 140 µM. Combining resveratrol treatment with ionizing radiation leads to significant reduction in cell viability and stem cell markers genes which was noticed with increasing the radiation dose when compared to ionizing radiation alone treated group.
Resveratrol has a radiosensitizing effect, that ability is triggered by reducing the expression of cancer stem cell markers and affecting EMT markers. Resveratrol showed to be a good candidate for further studies as anticancer drug in the treatment of human prostate cancer.</description><identifier>ISSN: 2476-762X</identifier><identifier>ISSN: 1513-7368</identifier><identifier>EISSN: 2476-762X</identifier><identifier>DOI: 10.31557/APJCP.2021.22.12.3823</identifier><identifier>PMID: 34967561</identifier><language>eng</language><publisher>Thailand: West Asia Organization for Cancer Prevention</publisher><subject>Biomarkers, Tumor - metabolism ; Cell Survival - drug effects ; Epithelial-Mesenchymal Transition - drug effects ; Humans ; Male ; Neoplastic Stem Cells - drug effects ; PC-3 Cells - drug effects ; Prostatic Neoplasms - drug therapy ; Radiation-Sensitizing Agents - pharmacology ; Resveratrol - pharmacology ; Signal Transduction - drug effects</subject><ispartof>Asian Pacific Journal of Cancer Prevention, 2021-12, Vol.22 (12), p.3823-3837</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3293-b77b2571b823199111aba3779909960cd8aa602bf46266be1ab830f7ff5e84e53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080384/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080384/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34967561$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Benhawy, Sanaa A</creatorcontrib><creatorcontrib>Morsi, Mohmed I</creatorcontrib><creatorcontrib>Fahmy, Enayat I</creatorcontrib><creatorcontrib>Soula, Moustafa A</creatorcontrib><creatorcontrib>Khalil, Fatma Al Zahraa Fh</creatorcontrib><creatorcontrib>Arab, Amal Refaat</creatorcontrib><title>Role of Resveratrol as Radiosensitizer by Targeting Cancer Stem Cells in Radioresistant Prostate Cancer Cells (PC-3)</title><title>Asian Pacific Journal of Cancer Prevention</title><addtitle>Asian Pac J Cancer Prev</addtitle><description>Here, we examined the role of resveratrol as a radiosensitizer by targeting cancer stem cells in radioresistant prostate cancer cells (PC-3) using stem cell markers CD44, CD49b and CD29, SOX2, OCT4, CXCR4, DCLK1 and EMT markers such as VIM and E-cadherin.
This study was an in vitro study involving PC-3 cell line which was dividing into four groups. Group I (CO): Control group composed of cells grown in the same medium without treatment with ionizing radiation or resveratrol. Group II (IR): Cells were treated with ionizing radiation alone. Group III (RV): Cells were treated with resveratrol alone. Group VI (IR&RV): The cells were treated with ionizing radiation and resveratrol in combination. The viability of cells was assessed by MTT assay. Genes of interest were measured by RT-PCR and the radiosensitizing efficacy of RV on proliferating cancer cells was determined by clonogenic assay.
Ionizing radiation significantly reduced PC-3 viability, lowered stem cell markers and affected epithelial to mesenchymal transition (EMT) genes expression at all doses (2, 4, 6 and 8 Gray). Resveratrol significantly decreased PC-3 viability and lowered stem cell markers and EMT genes expression at concentrations 35, 70 and 140 µM. Combining resveratrol treatment with ionizing radiation leads to significant reduction in cell viability and stem cell markers genes which was noticed with increasing the radiation dose when compared to ionizing radiation alone treated group.
Resveratrol has a radiosensitizing effect, that ability is triggered by reducing the expression of cancer stem cell markers and affecting EMT markers. Resveratrol showed to be a good candidate for further studies as anticancer drug in the treatment of human prostate cancer.</description><subject>Biomarkers, Tumor - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Epithelial-Mesenchymal Transition - drug effects</subject><subject>Humans</subject><subject>Male</subject><subject>Neoplastic Stem Cells - drug effects</subject><subject>PC-3 Cells - drug effects</subject><subject>Prostatic Neoplasms - drug therapy</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>Resveratrol - pharmacology</subject><subject>Signal Transduction - drug effects</subject><issn>2476-762X</issn><issn>1513-7368</issn><issn>2476-762X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUctOwzAQtBCIlscvIB_hkOBHYscXpCriKSSiAhI3y043xShNKttUgq8nUKjKaVc7O7OaHYROKEk5zXN5PqnuyiplhNGUsZSylBeM76Axy6RIpGAvu1v9CB2E8EZIlhcy30cjnikhc0HHKE77FnDf4CmEFXgTfd9iE_DUzFwfoAsuuk_w2H7gJ-PnEF03x6Xp6mH2GGGBS2jbgF23ZngILkTTRVz5fmgi_C2v906rMuFnR2ivMW2A4996iJ6vLp_Km-T-4fq2nNwnNWeKJ1ZKy3JJ7eCMKkUpNdZwKZUiSglSzwpjBGG2yQQTwsIAF5w0smlyKDLI-SG6WOsu3-0CZjV00ZtWL71bGP-he-P0f6Rzr3rer7QiBeFFNgiItUA9uAkemg2XEv2Tg_7JQX_noBnTlOnvHAbiyfblDe3v8fwL1nuF5A</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>El-Benhawy, Sanaa A</creator><creator>Morsi, Mohmed I</creator><creator>Fahmy, Enayat I</creator><creator>Soula, Moustafa A</creator><creator>Khalil, Fatma Al Zahraa Fh</creator><creator>Arab, Amal Refaat</creator><general>West Asia Organization for Cancer Prevention</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20211201</creationdate><title>Role of Resveratrol as Radiosensitizer by Targeting Cancer Stem Cells in Radioresistant Prostate Cancer Cells (PC-3)</title><author>El-Benhawy, Sanaa A ; Morsi, Mohmed I ; Fahmy, Enayat I ; Soula, Moustafa A ; Khalil, Fatma Al Zahraa Fh ; Arab, Amal Refaat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3293-b77b2571b823199111aba3779909960cd8aa602bf46266be1ab830f7ff5e84e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomarkers, Tumor - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Epithelial-Mesenchymal Transition - drug effects</topic><topic>Humans</topic><topic>Male</topic><topic>Neoplastic Stem Cells - drug effects</topic><topic>PC-3 Cells - drug effects</topic><topic>Prostatic Neoplasms - drug therapy</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>Resveratrol - pharmacology</topic><topic>Signal Transduction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Benhawy, Sanaa A</creatorcontrib><creatorcontrib>Morsi, Mohmed I</creatorcontrib><creatorcontrib>Fahmy, Enayat I</creatorcontrib><creatorcontrib>Soula, Moustafa A</creatorcontrib><creatorcontrib>Khalil, Fatma Al Zahraa Fh</creatorcontrib><creatorcontrib>Arab, Amal Refaat</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Asian Pacific Journal of Cancer Prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Benhawy, Sanaa A</au><au>Morsi, Mohmed I</au><au>Fahmy, Enayat I</au><au>Soula, Moustafa A</au><au>Khalil, Fatma Al Zahraa Fh</au><au>Arab, Amal Refaat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Resveratrol as Radiosensitizer by Targeting Cancer Stem Cells in Radioresistant Prostate Cancer Cells (PC-3)</atitle><jtitle>Asian Pacific Journal of Cancer Prevention</jtitle><addtitle>Asian Pac J Cancer Prev</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>22</volume><issue>12</issue><spage>3823</spage><epage>3837</epage><pages>3823-3837</pages><issn>2476-762X</issn><issn>1513-7368</issn><eissn>2476-762X</eissn><abstract>Here, we examined the role of resveratrol as a radiosensitizer by targeting cancer stem cells in radioresistant prostate cancer cells (PC-3) using stem cell markers CD44, CD49b and CD29, SOX2, OCT4, CXCR4, DCLK1 and EMT markers such as VIM and E-cadherin.
This study was an in vitro study involving PC-3 cell line which was dividing into four groups. Group I (CO): Control group composed of cells grown in the same medium without treatment with ionizing radiation or resveratrol. Group II (IR): Cells were treated with ionizing radiation alone. Group III (RV): Cells were treated with resveratrol alone. Group VI (IR&RV): The cells were treated with ionizing radiation and resveratrol in combination. The viability of cells was assessed by MTT assay. Genes of interest were measured by RT-PCR and the radiosensitizing efficacy of RV on proliferating cancer cells was determined by clonogenic assay.
Ionizing radiation significantly reduced PC-3 viability, lowered stem cell markers and affected epithelial to mesenchymal transition (EMT) genes expression at all doses (2, 4, 6 and 8 Gray). Resveratrol significantly decreased PC-3 viability and lowered stem cell markers and EMT genes expression at concentrations 35, 70 and 140 µM. Combining resveratrol treatment with ionizing radiation leads to significant reduction in cell viability and stem cell markers genes which was noticed with increasing the radiation dose when compared to ionizing radiation alone treated group.
Resveratrol has a radiosensitizing effect, that ability is triggered by reducing the expression of cancer stem cell markers and affecting EMT markers. Resveratrol showed to be a good candidate for further studies as anticancer drug in the treatment of human prostate cancer.</abstract><cop>Thailand</cop><pub>West Asia Organization for Cancer Prevention</pub><pmid>34967561</pmid><doi>10.31557/APJCP.2021.22.12.3823</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free E- Journals |
| subjects | Biomarkers, Tumor - metabolism Cell Survival - drug effects Epithelial-Mesenchymal Transition - drug effects Humans Male Neoplastic Stem Cells - drug effects PC-3 Cells - drug effects Prostatic Neoplasms - drug therapy Radiation-Sensitizing Agents - pharmacology Resveratrol - pharmacology Signal Transduction - drug effects |
| title | Role of Resveratrol as Radiosensitizer by Targeting Cancer Stem Cells in Radioresistant Prostate Cancer Cells (PC-3) |
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