Use of novel haptens in the production of antibodies for the detection of tryptamines
Tryptamines are a group of hallucinogenic drugs whose detection in body fluids could be simplified by immunochemical assay kits. Antibodies for these assays are obtained by the immunization of laboratory animals with conjugates of a hapten similar to the target analyte and a suitable protein. Theref...
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| Veröffentlicht in: | RSC advances 2018-01, Vol.8 (29), p.16243-16250 |
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| container_title | RSC advances |
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| creator | Maryška, Michal Fojtíková, Lucie Jurok, Radek Holubová, Barbora Lapčík, Oldřich Kuchař, Martin |
| description | Tryptamines are a group of hallucinogenic drugs whose detection in body fluids could be simplified by immunochemical assay kits. Antibodies for these assays are obtained by the immunization of laboratory animals with conjugates of a hapten similar to the target analyte and a suitable protein. Therefore we synthesized novel haptens derived from tryptamine-based drugs, with
,
-dimethyltryptamine (DMT), 5-methoxy-
,
-dimethyltryptamine (5-MeO-DMT) and
,
-diisopropyltryptamine (DiPT) selected as the target analytes. Their structures were modified with a short linker ended with a carboxylic group. The haptens were conjugated with bovine serum albumin (BSA) and rabbits were immunized with the conjugates. The obtained polyclonal antibodies showed good reactivity and the LOD of the constructed ELISAs was in the range 0.006-0.254 ng mL
. Thus, they are suitable for the development of immunochemical assay kits. |
| doi_str_mv | 10.1039/c8ra02528b |
| format | Article |
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,
-dimethyltryptamine (DMT), 5-methoxy-
,
-dimethyltryptamine (5-MeO-DMT) and
,
-diisopropyltryptamine (DiPT) selected as the target analytes. Their structures were modified with a short linker ended with a carboxylic group. The haptens were conjugated with bovine serum albumin (BSA) and rabbits were immunized with the conjugates. The obtained polyclonal antibodies showed good reactivity and the LOD of the constructed ELISAs was in the range 0.006-0.254 ng mL
. Thus, they are suitable for the development of immunochemical assay kits.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c8ra02528b</identifier><identifier>PMID: 35542213</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Antibodies ; Assaying ; Body fluids ; Chemistry ; Conjugates ; Immunization ; Immunoglobulins ; Laboratory animals ; Proteins ; Rabbits ; Serum albumin ; Tryptamines</subject><ispartof>RSC advances, 2018-01, Vol.8 (29), p.16243-16250</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2018</rights><rights>This journal is © The Royal Society of Chemistry 2018 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-9fa8287f953f54f04a1441569a32be9fcd97395960bee2ba230e56949b254f933</citedby><cites>FETCH-LOGICAL-c406t-9fa8287f953f54f04a1441569a32be9fcd97395960bee2ba230e56949b254f933</cites><orcidid>0000-0001-9593-9472 ; 0000-0002-1385-8167 ; 0000-0002-7616-6352</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080274/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080274/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35542213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maryška, Michal</creatorcontrib><creatorcontrib>Fojtíková, Lucie</creatorcontrib><creatorcontrib>Jurok, Radek</creatorcontrib><creatorcontrib>Holubová, Barbora</creatorcontrib><creatorcontrib>Lapčík, Oldřich</creatorcontrib><creatorcontrib>Kuchař, Martin</creatorcontrib><title>Use of novel haptens in the production of antibodies for the detection of tryptamines</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>Tryptamines are a group of hallucinogenic drugs whose detection in body fluids could be simplified by immunochemical assay kits. Antibodies for these assays are obtained by the immunization of laboratory animals with conjugates of a hapten similar to the target analyte and a suitable protein. Therefore we synthesized novel haptens derived from tryptamine-based drugs, with
,
-dimethyltryptamine (DMT), 5-methoxy-
,
-dimethyltryptamine (5-MeO-DMT) and
,
-diisopropyltryptamine (DiPT) selected as the target analytes. Their structures were modified with a short linker ended with a carboxylic group. The haptens were conjugated with bovine serum albumin (BSA) and rabbits were immunized with the conjugates. The obtained polyclonal antibodies showed good reactivity and the LOD of the constructed ELISAs was in the range 0.006-0.254 ng mL
. Thus, they are suitable for the development of immunochemical assay kits.</description><subject>Antibodies</subject><subject>Assaying</subject><subject>Body fluids</subject><subject>Chemistry</subject><subject>Conjugates</subject><subject>Immunization</subject><subject>Immunoglobulins</subject><subject>Laboratory animals</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Serum albumin</subject><subject>Tryptamines</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkVtLAzEQhYMoKtUXf4As-CJCNbdNNy9CLd6gIIh9DtndiY1skzXJFvrv3Wot1XmZgfNxZoaD0BnB1wQzeVMVQWOa06LcQ8cUczGkWMj9nfkIncb4gfsSOaGCHKIjluecUsKO0WwWIfMmc34JTTbXbQIXM-uyNIesDb7uqmS9WyPaJVv62kLMjA_fQA0JtnoKqzbphXUQT9CB0U2E000foNnD_dvkaTh9eXyejKfDimORhtLoghYjI3Nmcm4w14RzkgupGS1BmqqWIyZzKXAJQEtNGYZe5bKkPS4ZG6DbH9-2KxdQV-BS0I1qg13osFJeW_VXcXau3v1SSVxgOuK9weXGIPjPDmJSCxsraBrtwHdRUSH6Vbgg610X_9AP3wXXv6coZkzSkRRFT139UFXwMQYw22MIVuvA1KR4HX8HdtfD57vnb9HfeNgXGSWQYw</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Maryška, Michal</creator><creator>Fojtíková, Lucie</creator><creator>Jurok, Radek</creator><creator>Holubová, Barbora</creator><creator>Lapčík, Oldřich</creator><creator>Kuchař, Martin</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9593-9472</orcidid><orcidid>https://orcid.org/0000-0002-1385-8167</orcidid><orcidid>https://orcid.org/0000-0002-7616-6352</orcidid></search><sort><creationdate>20180101</creationdate><title>Use of novel haptens in the production of antibodies for the detection of tryptamines</title><author>Maryška, Michal ; Fojtíková, Lucie ; Jurok, Radek ; Holubová, Barbora ; Lapčík, Oldřich ; Kuchař, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-9fa8287f953f54f04a1441569a32be9fcd97395960bee2ba230e56949b254f933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antibodies</topic><topic>Assaying</topic><topic>Body fluids</topic><topic>Chemistry</topic><topic>Conjugates</topic><topic>Immunization</topic><topic>Immunoglobulins</topic><topic>Laboratory animals</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Serum albumin</topic><topic>Tryptamines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maryška, Michal</creatorcontrib><creatorcontrib>Fojtíková, Lucie</creatorcontrib><creatorcontrib>Jurok, Radek</creatorcontrib><creatorcontrib>Holubová, Barbora</creatorcontrib><creatorcontrib>Lapčík, Oldřich</creatorcontrib><creatorcontrib>Kuchař, Martin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maryška, Michal</au><au>Fojtíková, Lucie</au><au>Jurok, Radek</au><au>Holubová, Barbora</au><au>Lapčík, Oldřich</au><au>Kuchař, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of novel haptens in the production of antibodies for the detection of tryptamines</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>8</volume><issue>29</issue><spage>16243</spage><epage>16250</epage><pages>16243-16250</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Tryptamines are a group of hallucinogenic drugs whose detection in body fluids could be simplified by immunochemical assay kits. Antibodies for these assays are obtained by the immunization of laboratory animals with conjugates of a hapten similar to the target analyte and a suitable protein. Therefore we synthesized novel haptens derived from tryptamine-based drugs, with
,
-dimethyltryptamine (DMT), 5-methoxy-
,
-dimethyltryptamine (5-MeO-DMT) and
,
-diisopropyltryptamine (DiPT) selected as the target analytes. Their structures were modified with a short linker ended with a carboxylic group. The haptens were conjugated with bovine serum albumin (BSA) and rabbits were immunized with the conjugates. The obtained polyclonal antibodies showed good reactivity and the LOD of the constructed ELISAs was in the range 0.006-0.254 ng mL
. Thus, they are suitable for the development of immunochemical assay kits.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35542213</pmid><doi>10.1039/c8ra02528b</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9593-9472</orcidid><orcidid>https://orcid.org/0000-0002-1385-8167</orcidid><orcidid>https://orcid.org/0000-0002-7616-6352</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Antibodies Assaying Body fluids Chemistry Conjugates Immunization Immunoglobulins Laboratory animals Proteins Rabbits Serum albumin Tryptamines |
| title | Use of novel haptens in the production of antibodies for the detection of tryptamines |
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