The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin
Purpose To explore the role played by Glut-1 and H + /K + -ATPase in pepsin-induced, mouse laryngeal epithelial proliferation, growth, and development. Methods We established a mouse model of laryngopharyngeal reflux and measured Glut-1 and H + /K + -ATPase expression levels in mouse laryngeal epith...
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| Veröffentlicht in: | European archives of oto-rhino-laryngology 2022-06, Vol.279 (6), p.2981-2987 |
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| container_issue | 6 |
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| container_title | European archives of oto-rhino-laryngology |
| container_volume | 279 |
| creator | Ao, Yin-Jie Wu, Ting-Ting Cao, Zai-Zai Zhou, Shui-Hong Bao, Yang-Yang Shen, Li-Fang |
| description | Purpose
To explore the role played by Glut-1 and H
+
/K
+
-ATPase in pepsin-induced, mouse laryngeal epithelial proliferation, growth, and development.
Methods
We established a mouse model of laryngopharyngeal reflux and measured Glut-1 and H
+
/K
+
-ATPase expression levels in mouse laryngeal epithelium treated with artificial gastric juice containing pepsin.
Results
Artificial pepsin-containing gastric juice induced significant hyperplastic changes in mouse laryngeal epithelium compared to control mice at 15, 30, and 45 days. Inhibition of Glut-1 expression by 2-DG significantly suppressed such hyperplasia compared to mice exposed to artificial gastric juice containing pepsin at 15, 30, and 45 days. After treatment with pepsin-containing artificial gastric juice, RT-PCR and Western blotting showed that the levels of Glut-1 and H
+
/K
+
-ATPase α, β increased significantly.
Conclusions
Pepsin-containing artificial gastric juice promoted mouse laryngeal epithelial hyperplasia associated with abnormal expression of Glut-1 and H
+
/K
+
-ATPase α, β. |
| doi_str_mv | 10.1007/s00405-021-07221-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9072270</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2623330354</sourcerecordid><originalsourceid>FETCH-LOGICAL-c446t-36203939a8a2fffaf6125f26bc1643532c1a9513c12e74cb6e798ac6f17bfa743</originalsourceid><addsrcrecordid>eNp9kc1uFDEQhC0EIkvgBTggH5EiE_-NvXNBiiKSICLBYTlbHm8768hjD_YMYt8ebzZEcOFiH7qqulUfQm8Z_cAo1eeVUkk7QjkjVPP2qmdoxaSQRGqunqMV7YUmUmp9gl7Vek8p7WQvXqIT0dG16JhaobjZAS45As4eX8dlJgzbtMU3Z-dfzsjF5putgOHXVKDWkBMOCe_2E5Qp2hrswTQGBzjask93YCOGKcw7iGEZm3a7ONjiYY8nmGpIr9ELb2OFN4__Kfp-9WlzeUNuv15_vry4JU5KNROhOBW96O3acu-99YrxznM1OKak6AR3zPYdE45x0NINCnS_tk55pgdvtRSn6OMxd1qGEbYO0lxsNFMJY7vTZBvMv5MUduYu_zT9oUdNW8D7x4CSfyxQZzOG6iBGmyAv1XDFhRBUdIdd_Ch1JddawD-tYdQcMJkjJtMwmQdMRjXTu78PfLL84dIE4iiobdSKLeY-LyW10v4X-xsnqp4R</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2623330354</pqid></control><display><type>article</type><title>The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin</title><source>Springer Online Journals</source><creator>Ao, Yin-Jie ; Wu, Ting-Ting ; Cao, Zai-Zai ; Zhou, Shui-Hong ; Bao, Yang-Yang ; Shen, Li-Fang</creator><creatorcontrib>Ao, Yin-Jie ; Wu, Ting-Ting ; Cao, Zai-Zai ; Zhou, Shui-Hong ; Bao, Yang-Yang ; Shen, Li-Fang</creatorcontrib><description>Purpose
To explore the role played by Glut-1 and H
+
/K
+
-ATPase in pepsin-induced, mouse laryngeal epithelial proliferation, growth, and development.
Methods
We established a mouse model of laryngopharyngeal reflux and measured Glut-1 and H
+
/K
+
-ATPase expression levels in mouse laryngeal epithelium treated with artificial gastric juice containing pepsin.
Results
Artificial pepsin-containing gastric juice induced significant hyperplastic changes in mouse laryngeal epithelium compared to control mice at 15, 30, and 45 days. Inhibition of Glut-1 expression by 2-DG significantly suppressed such hyperplasia compared to mice exposed to artificial gastric juice containing pepsin at 15, 30, and 45 days. After treatment with pepsin-containing artificial gastric juice, RT-PCR and Western blotting showed that the levels of Glut-1 and H
+
/K
+
-ATPase α, β increased significantly.
Conclusions
Pepsin-containing artificial gastric juice promoted mouse laryngeal epithelial hyperplasia associated with abnormal expression of Glut-1 and H
+
/K
+
-ATPase α, β.</description><identifier>ISSN: 0937-4477</identifier><identifier>EISSN: 1434-4726</identifier><identifier>DOI: 10.1007/s00405-021-07221-6</identifier><identifier>PMID: 35083516</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Head and Neck Surgery ; Laryngology ; Medicine ; Medicine & Public Health ; Neurosurgery ; Otorhinolaryngology</subject><ispartof>European archives of oto-rhino-laryngology, 2022-06, Vol.279 (6), p.2981-2987</ispartof><rights>The Author(s) 2021</rights><rights>2021. The Author(s).</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-36203939a8a2fffaf6125f26bc1643532c1a9513c12e74cb6e798ac6f17bfa743</citedby><cites>FETCH-LOGICAL-c446t-36203939a8a2fffaf6125f26bc1643532c1a9513c12e74cb6e798ac6f17bfa743</cites><orcidid>0000-0002-7163-2289</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00405-021-07221-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00405-021-07221-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35083516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ao, Yin-Jie</creatorcontrib><creatorcontrib>Wu, Ting-Ting</creatorcontrib><creatorcontrib>Cao, Zai-Zai</creatorcontrib><creatorcontrib>Zhou, Shui-Hong</creatorcontrib><creatorcontrib>Bao, Yang-Yang</creatorcontrib><creatorcontrib>Shen, Li-Fang</creatorcontrib><title>The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin</title><title>European archives of oto-rhino-laryngology</title><addtitle>Eur Arch Otorhinolaryngol</addtitle><addtitle>Eur Arch Otorhinolaryngol</addtitle><description>Purpose
To explore the role played by Glut-1 and H
+
/K
+
-ATPase in pepsin-induced, mouse laryngeal epithelial proliferation, growth, and development.
Methods
We established a mouse model of laryngopharyngeal reflux and measured Glut-1 and H
+
/K
+
-ATPase expression levels in mouse laryngeal epithelium treated with artificial gastric juice containing pepsin.
Results
Artificial pepsin-containing gastric juice induced significant hyperplastic changes in mouse laryngeal epithelium compared to control mice at 15, 30, and 45 days. Inhibition of Glut-1 expression by 2-DG significantly suppressed such hyperplasia compared to mice exposed to artificial gastric juice containing pepsin at 15, 30, and 45 days. After treatment with pepsin-containing artificial gastric juice, RT-PCR and Western blotting showed that the levels of Glut-1 and H
+
/K
+
-ATPase α, β increased significantly.
Conclusions
Pepsin-containing artificial gastric juice promoted mouse laryngeal epithelial hyperplasia associated with abnormal expression of Glut-1 and H
+
/K
+
-ATPase α, β.</description><subject>Head and Neck Surgery</subject><subject>Laryngology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neurosurgery</subject><subject>Otorhinolaryngology</subject><issn>0937-4477</issn><issn>1434-4726</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kc1uFDEQhC0EIkvgBTggH5EiE_-NvXNBiiKSICLBYTlbHm8768hjD_YMYt8ebzZEcOFiH7qqulUfQm8Z_cAo1eeVUkk7QjkjVPP2qmdoxaSQRGqunqMV7YUmUmp9gl7Vek8p7WQvXqIT0dG16JhaobjZAS45As4eX8dlJgzbtMU3Z-dfzsjF5putgOHXVKDWkBMOCe_2E5Qp2hrswTQGBzjask93YCOGKcw7iGEZm3a7ONjiYY8nmGpIr9ELb2OFN4__Kfp-9WlzeUNuv15_vry4JU5KNROhOBW96O3acu-99YrxznM1OKak6AR3zPYdE45x0NINCnS_tk55pgdvtRSn6OMxd1qGEbYO0lxsNFMJY7vTZBvMv5MUduYu_zT9oUdNW8D7x4CSfyxQZzOG6iBGmyAv1XDFhRBUdIdd_Ch1JddawD-tYdQcMJkjJtMwmQdMRjXTu78PfLL84dIE4iiobdSKLeY-LyW10v4X-xsnqp4R</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Ao, Yin-Jie</creator><creator>Wu, Ting-Ting</creator><creator>Cao, Zai-Zai</creator><creator>Zhou, Shui-Hong</creator><creator>Bao, Yang-Yang</creator><creator>Shen, Li-Fang</creator><general>Springer Berlin Heidelberg</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7163-2289</orcidid></search><sort><creationdate>20220601</creationdate><title>The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin</title><author>Ao, Yin-Jie ; Wu, Ting-Ting ; Cao, Zai-Zai ; Zhou, Shui-Hong ; Bao, Yang-Yang ; Shen, Li-Fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-36203939a8a2fffaf6125f26bc1643532c1a9513c12e74cb6e798ac6f17bfa743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Head and Neck Surgery</topic><topic>Laryngology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neurosurgery</topic><topic>Otorhinolaryngology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ao, Yin-Jie</creatorcontrib><creatorcontrib>Wu, Ting-Ting</creatorcontrib><creatorcontrib>Cao, Zai-Zai</creatorcontrib><creatorcontrib>Zhou, Shui-Hong</creatorcontrib><creatorcontrib>Bao, Yang-Yang</creatorcontrib><creatorcontrib>Shen, Li-Fang</creatorcontrib><collection>Springer Nature Open Access Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>European archives of oto-rhino-laryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ao, Yin-Jie</au><au>Wu, Ting-Ting</au><au>Cao, Zai-Zai</au><au>Zhou, Shui-Hong</au><au>Bao, Yang-Yang</au><au>Shen, Li-Fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin</atitle><jtitle>European archives of oto-rhino-laryngology</jtitle><stitle>Eur Arch Otorhinolaryngol</stitle><addtitle>Eur Arch Otorhinolaryngol</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>279</volume><issue>6</issue><spage>2981</spage><epage>2987</epage><pages>2981-2987</pages><issn>0937-4477</issn><eissn>1434-4726</eissn><abstract>Purpose
To explore the role played by Glut-1 and H
+
/K
+
-ATPase in pepsin-induced, mouse laryngeal epithelial proliferation, growth, and development.
Methods
We established a mouse model of laryngopharyngeal reflux and measured Glut-1 and H
+
/K
+
-ATPase expression levels in mouse laryngeal epithelium treated with artificial gastric juice containing pepsin.
Results
Artificial pepsin-containing gastric juice induced significant hyperplastic changes in mouse laryngeal epithelium compared to control mice at 15, 30, and 45 days. Inhibition of Glut-1 expression by 2-DG significantly suppressed such hyperplasia compared to mice exposed to artificial gastric juice containing pepsin at 15, 30, and 45 days. After treatment with pepsin-containing artificial gastric juice, RT-PCR and Western blotting showed that the levels of Glut-1 and H
+
/K
+
-ATPase α, β increased significantly.
Conclusions
Pepsin-containing artificial gastric juice promoted mouse laryngeal epithelial hyperplasia associated with abnormal expression of Glut-1 and H
+
/K
+
-ATPase α, β.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35083516</pmid><doi>10.1007/s00405-021-07221-6</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7163-2289</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0937-4477 |
| ispartof | European archives of oto-rhino-laryngology, 2022-06, Vol.279 (6), p.2981-2987 |
| issn | 0937-4477 1434-4726 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9072270 |
| source | Springer Online Journals |
| subjects | Head and Neck Surgery Laryngology Medicine Medicine & Public Health Neurosurgery Otorhinolaryngology |
| title | The role of Glut-1 and H+/K+-ATPase expression in hyperplasia of mice laryngeal epithelium induced by pepsin |
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