The solid-state proton NMR study of bone using a dipolar filter: apatite hydroxyl content versus animal age
The hydroxyl content of bone apatite mineral has been measured using proton solid-state NMR performed with a multiple-pulse dipolar filter under slow magic angle spinning (MAS). This new method succeeded in resolving and relatively enhancing the main hydroxyl peak at 0 ppm from whole bone, making it...
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| Veröffentlicht in: | RSC advances 2019-05, Vol.9 (29), p.16909-16918 |
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| creator | Kaflak, Agnieszka Moskalewski, Stanisław Kolodziejski, Waclaw |
| description | The hydroxyl content of bone apatite mineral has been measured using proton solid-state NMR performed with a multiple-pulse dipolar filter under slow magic angle spinning (MAS). This new method succeeded in resolving and relatively enhancing the main hydroxyl peak at
0 ppm from whole bone, making it amenable to rigorous quantitative analysis. The proposed methodology, involving line fitting, the measurement of the apatite concentration in the studied material and adequate calibration, was proved to be convenient and suitable for monitoring bone mineral hydroxylation in different species and over the lifetime of the animal. It was found that the hydroxyl content in the cranial bone mineral of pig and rats remained in the 5-10% range, with reference to stoichiometric hydroxyapatite. In rats, the hydroxyl content showed a non-monotonic increase with age, which was governed by biological processes rather than by chemical, thermodynamically driven apatite maturation. |
| doi_str_mv | 10.1039/c9ra01902b |
| format | Article |
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0 ppm from whole bone, making it amenable to rigorous quantitative analysis. The proposed methodology, involving line fitting, the measurement of the apatite concentration in the studied material and adequate calibration, was proved to be convenient and suitable for monitoring bone mineral hydroxylation in different species and over the lifetime of the animal. It was found that the hydroxyl content in the cranial bone mineral of pig and rats remained in the 5-10% range, with reference to stoichiometric hydroxyapatite. In rats, the hydroxyl content showed a non-monotonic increase with age, which was governed by biological processes rather than by chemical, thermodynamically driven apatite maturation.</description><subject>Apatite</subject><subject>Biological activity</subject><subject>Biomedical materials</subject><subject>Chemistry</subject><subject>Hydroxyapatite</subject><subject>Hydroxylation</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Organic chemistry</subject><subject>Quantitative analysis</subject><subject>Solid state</subject><subject>Surgical implants</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkV1LHDEUhkOpqKg3_oAS6E0Rps3HTHbTi4JdtAq2BdHrkMmc7MZmk2mSEfffN9YP1BBIIE_ePCcHoUNKPlPC5RcjkyZUEta_Q7uMtKJhRMj3L_Y76CDnG1KH6CgTdBvt8K6jgs_ILvpztQKco3dDk4sugMcUSwz4189LnMs0bHC0uI8B8JRdWGKNBzdGrxO2zhdIX7EedXH14mozpHi38djEUCAUfAspTxnr4NbaY72EfbRltc9w8LjuoevTk6vFWXPx-8f54viiMVWpNFZwMxgjSC8EZWYmJGs5mQtJregt72CQAoDz2cDMvJbeakt0D10rjIVWzvke-vaQO079GgZTZZL2akxVJG1U1E69PglupZbxVkki2paLGvDpMSDFvxPkotYuG_BeB4hTVqyKkXlF79_6-Aa9iVMKtTzFWP1mViep1NEDZVLMOYF9lqFE3bdRLeTl8f82fq_wh5f6z-hT0_g__e2Y4Q</recordid><startdate>20190529</startdate><enddate>20190529</enddate><creator>Kaflak, Agnieszka</creator><creator>Moskalewski, Stanisław</creator><creator>Kolodziejski, Waclaw</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6864-2144</orcidid></search><sort><creationdate>20190529</creationdate><title>The solid-state proton NMR study of bone using a dipolar filter: apatite hydroxyl content versus animal age</title><author>Kaflak, Agnieszka ; Moskalewski, Stanisław ; Kolodziejski, Waclaw</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-f63cdcc60b6612c76924308691f6bf35ed96ee337d2c80194af0abe546cfe4983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apatite</topic><topic>Biological activity</topic><topic>Biomedical materials</topic><topic>Chemistry</topic><topic>Hydroxyapatite</topic><topic>Hydroxylation</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Organic chemistry</topic><topic>Quantitative analysis</topic><topic>Solid state</topic><topic>Surgical implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaflak, Agnieszka</creatorcontrib><creatorcontrib>Moskalewski, Stanisław</creatorcontrib><creatorcontrib>Kolodziejski, Waclaw</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaflak, Agnieszka</au><au>Moskalewski, Stanisław</au><au>Kolodziejski, Waclaw</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The solid-state proton NMR study of bone using a dipolar filter: apatite hydroxyl content versus animal age</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2019-05-29</date><risdate>2019</risdate><volume>9</volume><issue>29</issue><spage>16909</spage><epage>16918</epage><pages>16909-16918</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>The hydroxyl content of bone apatite mineral has been measured using proton solid-state NMR performed with a multiple-pulse dipolar filter under slow magic angle spinning (MAS). This new method succeeded in resolving and relatively enhancing the main hydroxyl peak at
0 ppm from whole bone, making it amenable to rigorous quantitative analysis. The proposed methodology, involving line fitting, the measurement of the apatite concentration in the studied material and adequate calibration, was proved to be convenient and suitable for monitoring bone mineral hydroxylation in different species and over the lifetime of the animal. It was found that the hydroxyl content in the cranial bone mineral of pig and rats remained in the 5-10% range, with reference to stoichiometric hydroxyapatite. In rats, the hydroxyl content showed a non-monotonic increase with age, which was governed by biological processes rather than by chemical, thermodynamically driven apatite maturation.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35516370</pmid><doi>10.1039/c9ra01902b</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6864-2144</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Apatite Biological activity Biomedical materials Chemistry Hydroxyapatite Hydroxylation NMR Nuclear magnetic resonance Organic chemistry Quantitative analysis Solid state Surgical implants |
| title | The solid-state proton NMR study of bone using a dipolar filter: apatite hydroxyl content versus animal age |
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