Investigation of the detoxification effect of licorice on Semen Strychni -induced acute toxicity in rats using a HPLC-Q-TOF/MS-based metabolomics approach
, a traditional Chinese medicine (TCM), has been widely used to treat paraplegia, facial nerve palsy and myasthenia gravis. However, its clinical application is greatly limited due to its fatal toxicity. To investigate the acute toxicity of and the detoxification effect of , a high-performance liqui...
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| Veröffentlicht in: | RSC advances 2020-12, Vol.10 (72), p.44398-44407 |
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| creator | Xiang, Yalan Wang, Chao Wen, Jing Zhang, Min Duan, Xiaoyu Wang, Lu Yan, Miao Li, Huande Fang, Pingfei |
| description | , a traditional Chinese medicine (TCM), has been widely used to treat paraplegia, facial nerve palsy and myasthenia gravis. However, its clinical application is greatly limited due to its fatal toxicity. To investigate the acute toxicity of
and the detoxification effect of
, a high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS) based urinary metabolomics method was developed in this study. After intraperitoneal injection to rats with
extract, the serum biochemical indexes were changed significantly, the liver and kidney showed severe necrosis and edema. Then the poisoned rat model was subsequently used for metabolomics research. Through principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), we finally identified 19 endogenous differential metabolites involved in amino acid metabolism, glycerophospholipid metabolism, tricarboxylic acid (TCA) cycle, oxidative stress and energy metabolism. In addition, 4 exogenous compounds from
(3 prototypes and 1 metabolite) were also identified in the present study. Results showed that the alterations of 23 compounds caused by
were significantly reversed after
treatment, which indicated that restoring the endogenous metabolic disorder and accelerating the metabolism of the main toxic components might be the possible detoxification mechanisms of
. This study may provide an integral understanding for the acute toxicity of
and the detoxification effect of
, thereby contributing to the clinical use of
and
. |
| doi_str_mv | 10.1039/d0ra08568e |
| format | Article |
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and the detoxification effect of
, a high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS) based urinary metabolomics method was developed in this study. After intraperitoneal injection to rats with
extract, the serum biochemical indexes were changed significantly, the liver and kidney showed severe necrosis and edema. Then the poisoned rat model was subsequently used for metabolomics research. Through principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), we finally identified 19 endogenous differential metabolites involved in amino acid metabolism, glycerophospholipid metabolism, tricarboxylic acid (TCA) cycle, oxidative stress and energy metabolism. In addition, 4 exogenous compounds from
(3 prototypes and 1 metabolite) were also identified in the present study. Results showed that the alterations of 23 compounds caused by
were significantly reversed after
treatment, which indicated that restoring the endogenous metabolic disorder and accelerating the metabolism of the main toxic components might be the possible detoxification mechanisms of
. This study may provide an integral understanding for the acute toxicity of
and the detoxification effect of
, thereby contributing to the clinical use of
and
.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d0ra08568e</identifier><identifier>PMID: 35517145</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Chemistry ; Discriminant analysis ; Edema ; High performance liquid chromatography ; Mass spectrometry ; Metabolic disorders ; Metabolism ; Metabolites ; Necrosis ; Paraplegics ; Performance indices ; Principal components analysis ; Quadrupoles ; Toxicity ; Traditional Chinese medicine</subject><ispartof>RSC advances, 2020-12, Vol.10 (72), p.44398-44407</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2020</rights><rights>This journal is © The Royal Society of Chemistry 2020 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-ff2a3c112d082363f233d965afb4c7975f95ed538e2130ee246ff76c976bcdf23</citedby><cites>FETCH-LOGICAL-c406t-ff2a3c112d082363f233d965afb4c7975f95ed538e2130ee246ff76c976bcdf23</cites><orcidid>0000-0002-4948-5497 ; 0000-0002-7204-8631 ; 0000-0001-9593-9739 ; 0000-0003-2672-9206 ; 0000-0003-3713-1488 ; 0000-0002-2431-5934 ; 0000-0002-6278-5267 ; 0000-0002-0409-9146</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058463/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058463/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35517145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiang, Yalan</creatorcontrib><creatorcontrib>Wang, Chao</creatorcontrib><creatorcontrib>Wen, Jing</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Duan, Xiaoyu</creatorcontrib><creatorcontrib>Wang, Lu</creatorcontrib><creatorcontrib>Yan, Miao</creatorcontrib><creatorcontrib>Li, Huande</creatorcontrib><creatorcontrib>Fang, Pingfei</creatorcontrib><title>Investigation of the detoxification effect of licorice on Semen Strychni -induced acute toxicity in rats using a HPLC-Q-TOF/MS-based metabolomics approach</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>, a traditional Chinese medicine (TCM), has been widely used to treat paraplegia, facial nerve palsy and myasthenia gravis. However, its clinical application is greatly limited due to its fatal toxicity. To investigate the acute toxicity of
and the detoxification effect of
, a high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS) based urinary metabolomics method was developed in this study. After intraperitoneal injection to rats with
extract, the serum biochemical indexes were changed significantly, the liver and kidney showed severe necrosis and edema. Then the poisoned rat model was subsequently used for metabolomics research. Through principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), we finally identified 19 endogenous differential metabolites involved in amino acid metabolism, glycerophospholipid metabolism, tricarboxylic acid (TCA) cycle, oxidative stress and energy metabolism. In addition, 4 exogenous compounds from
(3 prototypes and 1 metabolite) were also identified in the present study. Results showed that the alterations of 23 compounds caused by
were significantly reversed after
treatment, which indicated that restoring the endogenous metabolic disorder and accelerating the metabolism of the main toxic components might be the possible detoxification mechanisms of
. This study may provide an integral understanding for the acute toxicity of
and the detoxification effect of
, thereby contributing to the clinical use of
and
.</description><subject>Chemistry</subject><subject>Discriminant analysis</subject><subject>Edema</subject><subject>High performance liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Metabolic disorders</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Necrosis</subject><subject>Paraplegics</subject><subject>Performance indices</subject><subject>Principal components analysis</subject><subject>Quadrupoles</subject><subject>Toxicity</subject><subject>Traditional Chinese medicine</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpVkc9O3DAQxq2qVUGUCw9QWeqtUor_xE5yqYQWKEiLoAXOlmOPd4028dZ2UPdV-rT1dgGBD2Nr5jefR_MhdETJN0p4d2xJ1KQVsoV3aJ-RWlaMyO79q_ceOkzpgZQjBWWSfkR7XAja0Frso7-X4yOk7Bc6-zDi4HBeAraQwx_vvNllwTkweVtceROiN4BL9hYGKDHHjVmOHld-tJMBi7WZMuCtgPF5g_2Io84JT8mPC6zxxc18Vv2s7q7Pj69uq16n0jJA1n1YhcGbhPV6HYM2y0_og9OrBIdP9wG6Pz-7m11U8-sfl7OTeWVqInPlHNPcUMosaRmX3DHObSeFdn1tmq4RrhNgBW-BUU4AWC2da6TpGtkbW-gD9H2nu576AayBMUe9UuvoBx03Kmiv3lZGv1SL8Kg6Itpa8iLw5Ukght9T2aZ6CFMcy8yK1Q1pG85YW6ivO8rEkFIE9_IDJWprpTolv07-W3lW4M-vZ3pBn43j_wA62Jvp</recordid><startdate>20201216</startdate><enddate>20201216</enddate><creator>Xiang, Yalan</creator><creator>Wang, Chao</creator><creator>Wen, Jing</creator><creator>Zhang, Min</creator><creator>Duan, Xiaoyu</creator><creator>Wang, Lu</creator><creator>Yan, Miao</creator><creator>Li, Huande</creator><creator>Fang, Pingfei</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4948-5497</orcidid><orcidid>https://orcid.org/0000-0002-7204-8631</orcidid><orcidid>https://orcid.org/0000-0001-9593-9739</orcidid><orcidid>https://orcid.org/0000-0003-2672-9206</orcidid><orcidid>https://orcid.org/0000-0003-3713-1488</orcidid><orcidid>https://orcid.org/0000-0002-2431-5934</orcidid><orcidid>https://orcid.org/0000-0002-6278-5267</orcidid><orcidid>https://orcid.org/0000-0002-0409-9146</orcidid></search><sort><creationdate>20201216</creationdate><title>Investigation of the detoxification effect of licorice on Semen Strychni -induced acute toxicity in rats using a HPLC-Q-TOF/MS-based metabolomics approach</title><author>Xiang, Yalan ; Wang, Chao ; Wen, Jing ; Zhang, Min ; Duan, Xiaoyu ; Wang, Lu ; Yan, Miao ; Li, Huande ; Fang, Pingfei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-ff2a3c112d082363f233d965afb4c7975f95ed538e2130ee246ff76c976bcdf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Chemistry</topic><topic>Discriminant analysis</topic><topic>Edema</topic><topic>High performance liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Metabolic disorders</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Necrosis</topic><topic>Paraplegics</topic><topic>Performance indices</topic><topic>Principal components analysis</topic><topic>Quadrupoles</topic><topic>Toxicity</topic><topic>Traditional Chinese medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiang, Yalan</creatorcontrib><creatorcontrib>Wang, Chao</creatorcontrib><creatorcontrib>Wen, Jing</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Duan, Xiaoyu</creatorcontrib><creatorcontrib>Wang, Lu</creatorcontrib><creatorcontrib>Yan, Miao</creatorcontrib><creatorcontrib>Li, Huande</creatorcontrib><creatorcontrib>Fang, Pingfei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiang, Yalan</au><au>Wang, Chao</au><au>Wen, Jing</au><au>Zhang, Min</au><au>Duan, Xiaoyu</au><au>Wang, Lu</au><au>Yan, Miao</au><au>Li, Huande</au><au>Fang, Pingfei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the detoxification effect of licorice on Semen Strychni -induced acute toxicity in rats using a HPLC-Q-TOF/MS-based metabolomics approach</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2020-12-16</date><risdate>2020</risdate><volume>10</volume><issue>72</issue><spage>44398</spage><epage>44407</epage><pages>44398-44407</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>, a traditional Chinese medicine (TCM), has been widely used to treat paraplegia, facial nerve palsy and myasthenia gravis. However, its clinical application is greatly limited due to its fatal toxicity. To investigate the acute toxicity of
and the detoxification effect of
, a high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS) based urinary metabolomics method was developed in this study. After intraperitoneal injection to rats with
extract, the serum biochemical indexes were changed significantly, the liver and kidney showed severe necrosis and edema. Then the poisoned rat model was subsequently used for metabolomics research. Through principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), we finally identified 19 endogenous differential metabolites involved in amino acid metabolism, glycerophospholipid metabolism, tricarboxylic acid (TCA) cycle, oxidative stress and energy metabolism. In addition, 4 exogenous compounds from
(3 prototypes and 1 metabolite) were also identified in the present study. Results showed that the alterations of 23 compounds caused by
were significantly reversed after
treatment, which indicated that restoring the endogenous metabolic disorder and accelerating the metabolism of the main toxic components might be the possible detoxification mechanisms of
. This study may provide an integral understanding for the acute toxicity of
and the detoxification effect of
, thereby contributing to the clinical use of
and
.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35517145</pmid><doi>10.1039/d0ra08568e</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4948-5497</orcidid><orcidid>https://orcid.org/0000-0002-7204-8631</orcidid><orcidid>https://orcid.org/0000-0001-9593-9739</orcidid><orcidid>https://orcid.org/0000-0003-2672-9206</orcidid><orcidid>https://orcid.org/0000-0003-3713-1488</orcidid><orcidid>https://orcid.org/0000-0002-2431-5934</orcidid><orcidid>https://orcid.org/0000-0002-6278-5267</orcidid><orcidid>https://orcid.org/0000-0002-0409-9146</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | RSC advances, 2020-12, Vol.10 (72), p.44398-44407 |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Chemistry Discriminant analysis Edema High performance liquid chromatography Mass spectrometry Metabolic disorders Metabolism Metabolites Necrosis Paraplegics Performance indices Principal components analysis Quadrupoles Toxicity Traditional Chinese medicine |
| title | Investigation of the detoxification effect of licorice on Semen Strychni -induced acute toxicity in rats using a HPLC-Q-TOF/MS-based metabolomics approach |
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