PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants

Summary Adenine base editors (ABEs), which are generally engineered adenosine deaminases and Cas variants, introduce site‐specific A‐to‐G mutations for agronomic trait improvement. However, notably varying editing efficiencies, restrictive requirements for protospacer‐adjacent motifs (PAMs) and a na...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Plant biotechnology journal 2022-05, Vol.20 (5), p.934-943
Hauptverfasser:	Tan, Jiantao, Zeng, Dongchang, Zhao, Yanchang, Wang, Yaxi, Liu, Taoli, Li, Shuangchun, Xue, Yang, Luo, Yuyu, Xie, Xianrong, Chen, Letian, Liu, Yao‐Guang, Zhu, Qinlong
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine adenine base editors Adenosine Adenosine deaminase Cas9 variants CRISPR CRISPR-Associated Protein 9 - genetics CRISPR-Cas Systems - genetics Crop improvement Editing Efficiency Gene Editing - methods Genome, Plant Genomes Genomics Localization Mutation Rice single‐stranded DNA‐binding domain (DBD) TadA8e
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!