The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia
Diarrhoeagenic verotoxin producing non-O157 (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and chara...
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| Veröffentlicht in: | The Malaysian journal of medical sciences 2022-04, Vol.29 (2), p.55-68 |
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| creator | Karim Khan, Md Fazlul Rashid, Shah Samiur Ramli, Aizi Nor Mazila Kabir, Muhammad Nomani Hasan Maziz, Mohammad Nazmul |
| description | Diarrhoeagenic verotoxin producing non-O157
(VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children.
Standard microbiological techniques identified a total of 137 non-repeated, clinically significant
isolates. Serological assays discerned non-O157
serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR).
Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157
. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing
, genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates.
This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children. |
| doi_str_mv | 10.21315/mjms2022.29.2.6 |
| format | Article |
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(VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children.
Standard microbiological techniques identified a total of 137 non-repeated, clinically significant
isolates. Serological assays discerned non-O157
serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR).
Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157
. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing
, genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates.
This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children.</description><identifier>ISSN: 1394-195X</identifier><identifier>EISSN: 2180-4303</identifier><identifier>DOI: 10.21315/mjms2022.29.2.6</identifier><identifier>PMID: 35528808</identifier><language>eng</language><publisher>Malaysia: Universiti Sains Malaysia Press</publisher><subject>Antibiotics ; Developing countries ; Diarrhea ; Drug resistance ; E coli ; Food contamination & poisoning ; Genes ; Illnesses ; Infections ; Inflammatory bowel disease ; Laboratories ; LDCs ; Molecular weight ; Mortality ; Original ; Polymerase chain reaction ; Public health ; Serology ; Statistical analysis ; Virulence</subject><ispartof>The Malaysian journal of medical sciences, 2022-04, Vol.29 (2), p.55-68</ispartof><rights>Penerbit Universiti Sains Malaysia, 2022.</rights><rights>Copyright Universiti Sains Malaysia Press Mar/Apr 2022</rights><rights>Penerbit Universiti Sains Malaysia, 2022 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036922/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036922/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35528808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karim Khan, Md Fazlul</creatorcontrib><creatorcontrib>Rashid, Shah Samiur</creatorcontrib><creatorcontrib>Ramli, Aizi Nor Mazila</creatorcontrib><creatorcontrib>Kabir, Muhammad Nomani</creatorcontrib><creatorcontrib>Hasan Maziz, Mohammad Nazmul</creatorcontrib><creatorcontrib>Faculty of Industrial Sciences Technology, Universiti Malaysia Pahang, Pahang, Malaysia</creatorcontrib><creatorcontrib>Faculty of Medicine, Bioscience and Nursing, MAHSA University, Selangor, Malaysia</creatorcontrib><creatorcontrib>Department of Computer Science and Engineering, Trust University Barishal, Barishal, Bangladesh</creatorcontrib><title>The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia</title><title>The Malaysian journal of medical sciences</title><addtitle>Malays J Med Sci</addtitle><description>Diarrhoeagenic verotoxin producing non-O157
(VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children.
Standard microbiological techniques identified a total of 137 non-repeated, clinically significant
isolates. Serological assays discerned non-O157
serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR).
Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157
. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing
, genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates.
This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children.</description><subject>Antibiotics</subject><subject>Developing countries</subject><subject>Diarrhea</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Food contamination & poisoning</subject><subject>Genes</subject><subject>Illnesses</subject><subject>Infections</subject><subject>Inflammatory bowel disease</subject><subject>Laboratories</subject><subject>LDCs</subject><subject>Molecular weight</subject><subject>Mortality</subject><subject>Original</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>Serology</subject><subject>Statistical analysis</subject><subject>Virulence</subject><issn>1394-195X</issn><issn>2180-4303</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkc1v1DAQxS0EotvCnROyxIUDWcbj2HEuSNVSPkShHAqCk-U43o1XiV3spKIX_nYs-iHgMnOY957m6UfIEwZrZJyJl9N-ygiIa2zXuJb3yAqZgqrmwO-TFeNtXbFWfDsghznvAbhAqR6SAy4EKgVqRX6dD45uBpOMnV3y2cw-Bhq39LU3KQ3RmZ0L3tKvLsU5_vSBfk6xX6wPO_ophuqMiYaeZDsUsx28oTaOnpoplvv3uJS5GfzYJxdo8X5YTJhNeEE_mtFcZW8ekQdbM2b3-GYfkS9vTs4376rTs7fvN8enleXQzFXfg7MAHQrroAFl2RY5ctHWoFzbKWM5FxK7XkItVCcbrLeKu05YVbPeWX5EXl3nXizd5HrrwpzMqC-Sn0y60tF4_e8l-EHv4qVugcsWsQQ8vwlI8cfi8qwnn60bRxNcXLJGKVmtpKxVkT77T7qPSwqlnsamMGgVNLyo4FplU8w5ue3dMwz0H7j6Fq7GVqOWxfL07xJ3hlua_DesLKIk</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Karim Khan, Md Fazlul</creator><creator>Rashid, Shah Samiur</creator><creator>Ramli, Aizi Nor Mazila</creator><creator>Kabir, Muhammad Nomani</creator><creator>Hasan Maziz, Mohammad Nazmul</creator><general>Universiti Sains Malaysia Press</general><general>Penerbit Universiti Sains Malaysia</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220401</creationdate><title>The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia</title><author>Karim Khan, Md Fazlul ; 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(VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children.
Standard microbiological techniques identified a total of 137 non-repeated, clinically significant
isolates. Serological assays discerned non-O157
serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR).
Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157
. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing
, genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates.
This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children.</abstract><cop>Malaysia</cop><pub>Universiti Sains Malaysia Press</pub><pmid>35528808</pmid><doi>10.21315/mjms2022.29.2.6</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Malaysian journal of medical sciences, 2022-04, Vol.29 (2), p.55-68 |
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| source | Bioline International; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antibiotics Developing countries Diarrhea Drug resistance E coli Food contamination & poisoning Genes Illnesses Infections Inflammatory bowel disease Laboratories LDCs Molecular weight Mortality Original Polymerase chain reaction Public health Serology Statistical analysis Virulence |
| title | The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia |
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