Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx
Traditional Chinese medicine PaoTianXiong (PTX) is a processed product of Debx. with polysaccharide as the main ingredient. The properties of PTX polysaccharide (PTXP) may be affected by different extraction methods. To develop and utilize PTXP better, it is of great significance to study the extrac...
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| Veröffentlicht in: | RSC advances 2021-06, Vol.11 (35), p.21259-21268 |
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| creator | Qiu, Kuncheng Li, Zunjiang Long, Yingxin Lu, Zhongyu Zhu, Wei |
| description | Traditional Chinese medicine PaoTianXiong (PTX) is a processed product of
Debx. with polysaccharide as the main ingredient. The properties of PTX polysaccharide (PTXP) may be affected by different extraction methods. To develop and utilize PTXP better, it is of great significance to study the extraction methods of PTXP. Thus, we extracted PTXPs with dilute alkaline water extraction, ultrasound-assisted extraction, cellulase-assisted extraction, and hot water extraction (HWE), respectively. The characterizations of PTXPs extracted by different methods were analyzed based on purity determination, infrared analysis, molecular weight and monosaccharide composition. And antioxidant experiments of PTXPs were conducted. The results showed that PTXPs extracted by the four extraction methods were all glucan. After purification, the PTXPs showed similar antioxidant activity
. The molecular weight of polysaccharides extracted by the cellulase-assisted method was different from that extracted by the other three methods. Our results showed that not only the yield but also the effect of extraction methods on the properties of PTXP should be considered when selecting the best extraction method. Therefore, HWE was considered to be the best extraction method of PTXP. The yield and purity of purified PTXP were 24.5% and 97.1%, respectively. The optimized extraction conditions were: an extraction temperature of 90 °C, extraction time of 2.17 h, solid-liquid ratio of 1 : 29 (g mL
), and number of extractions of 2. |
| doi_str_mv | 10.1039/d1ra03628a |
| format | Article |
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Debx. with polysaccharide as the main ingredient. The properties of PTX polysaccharide (PTXP) may be affected by different extraction methods. To develop and utilize PTXP better, it is of great significance to study the extraction methods of PTXP. Thus, we extracted PTXPs with dilute alkaline water extraction, ultrasound-assisted extraction, cellulase-assisted extraction, and hot water extraction (HWE), respectively. The characterizations of PTXPs extracted by different methods were analyzed based on purity determination, infrared analysis, molecular weight and monosaccharide composition. And antioxidant experiments of PTXPs were conducted. The results showed that PTXPs extracted by the four extraction methods were all glucan. After purification, the PTXPs showed similar antioxidant activity
. The molecular weight of polysaccharides extracted by the cellulase-assisted method was different from that extracted by the other three methods. Our results showed that not only the yield but also the effect of extraction methods on the properties of PTXP should be considered when selecting the best extraction method. Therefore, HWE was considered to be the best extraction method of PTXP. The yield and purity of purified PTXP were 24.5% and 97.1%, respectively. The optimized extraction conditions were: an extraction temperature of 90 °C, extraction time of 2.17 h, solid-liquid ratio of 1 : 29 (g mL
), and number of extractions of 2.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d1ra03628a</identifier><identifier>PMID: 35478822</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Antioxidants ; Cellulase ; Chemistry ; Glucan ; Infrared analysis ; Molecular weight ; Monosaccharides ; Polysaccharides ; Purity ; Traditional Chinese medicine</subject><ispartof>RSC advances, 2021-06, Vol.11 (35), p.21259-21268</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2021</rights><rights>This journal is © The Royal Society of Chemistry 2021 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c336t-a22760ec296918cdea7ab1411e50f50ef906062b331b34eb51ae4accac8a4a063</citedby><cites>FETCH-LOGICAL-c336t-a22760ec296918cdea7ab1411e50f50ef906062b331b34eb51ae4accac8a4a063</cites><orcidid>0000-0003-1165-634X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034042/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034042/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35478822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Kuncheng</creatorcontrib><creatorcontrib>Li, Zunjiang</creatorcontrib><creatorcontrib>Long, Yingxin</creatorcontrib><creatorcontrib>Lu, Zhongyu</creatorcontrib><creatorcontrib>Zhu, Wei</creatorcontrib><title>Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>Traditional Chinese medicine PaoTianXiong (PTX) is a processed product of
Debx. with polysaccharide as the main ingredient. The properties of PTX polysaccharide (PTXP) may be affected by different extraction methods. To develop and utilize PTXP better, it is of great significance to study the extraction methods of PTXP. Thus, we extracted PTXPs with dilute alkaline water extraction, ultrasound-assisted extraction, cellulase-assisted extraction, and hot water extraction (HWE), respectively. The characterizations of PTXPs extracted by different methods were analyzed based on purity determination, infrared analysis, molecular weight and monosaccharide composition. And antioxidant experiments of PTXPs were conducted. The results showed that PTXPs extracted by the four extraction methods were all glucan. After purification, the PTXPs showed similar antioxidant activity
. The molecular weight of polysaccharides extracted by the cellulase-assisted method was different from that extracted by the other three methods. Our results showed that not only the yield but also the effect of extraction methods on the properties of PTXP should be considered when selecting the best extraction method. Therefore, HWE was considered to be the best extraction method of PTXP. The yield and purity of purified PTXP were 24.5% and 97.1%, respectively. The optimized extraction conditions were: an extraction temperature of 90 °C, extraction time of 2.17 h, solid-liquid ratio of 1 : 29 (g mL
), and number of extractions of 2.</description><subject>Antioxidants</subject><subject>Cellulase</subject><subject>Chemistry</subject><subject>Glucan</subject><subject>Infrared analysis</subject><subject>Molecular weight</subject><subject>Monosaccharides</subject><subject>Polysaccharides</subject><subject>Purity</subject><subject>Traditional Chinese medicine</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkd1LHDEUxYNYuov60j9AAr5IYTVfk515KSxaP0AoaPsc7iR33MjMZE1mivvfN7uuYr0vOZDfPZzLIeQbZ2ecyerc8QhMalHCHpkKpvRMMF3tf9ATcpTSE8ujCy40_0omslDzshRiStzDMLo1DT3FlyGCHXyWHQ7L4BINDV2Fdp3A2iVE7zDRJoaOAl3FYDEldBvlRjts2IUNvR_GjlqInc8r2Hp6ifXLIfnSQJvwaPcekD9XP39f3Mzufl3fXizuZlZKPcxAiLlmaEWlK15ahzCHmivOsWBNwbCpmGZa1FLyWiqsCw6ocjawJShgWh6QH6--q7Hu0Fns80mtWUXfQVybAN78_9P7pXkMf03FpGJKZIPTnUEMzyOmwXQ-WWxb6DGMyQhd6LnSQpcZPfmEPoUx9vk8IwqlFBNqa_j9lbIxpBSxeQ_Dmdn0Zy75_WLb3yLDxx_jv6Nvbcl_jAOXKg</recordid><startdate>20210616</startdate><enddate>20210616</enddate><creator>Qiu, Kuncheng</creator><creator>Li, Zunjiang</creator><creator>Long, Yingxin</creator><creator>Lu, Zhongyu</creator><creator>Zhu, Wei</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1165-634X</orcidid></search><sort><creationdate>20210616</creationdate><title>Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx</title><author>Qiu, Kuncheng ; Li, Zunjiang ; Long, Yingxin ; Lu, Zhongyu ; Zhu, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-a22760ec296918cdea7ab1411e50f50ef906062b331b34eb51ae4accac8a4a063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antioxidants</topic><topic>Cellulase</topic><topic>Chemistry</topic><topic>Glucan</topic><topic>Infrared analysis</topic><topic>Molecular weight</topic><topic>Monosaccharides</topic><topic>Polysaccharides</topic><topic>Purity</topic><topic>Traditional Chinese medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Kuncheng</creatorcontrib><creatorcontrib>Li, Zunjiang</creatorcontrib><creatorcontrib>Long, Yingxin</creatorcontrib><creatorcontrib>Lu, Zhongyu</creatorcontrib><creatorcontrib>Zhu, Wei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Kuncheng</au><au>Li, Zunjiang</au><au>Long, Yingxin</au><au>Lu, Zhongyu</au><au>Zhu, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2021-06-16</date><risdate>2021</risdate><volume>11</volume><issue>35</issue><spage>21259</spage><epage>21268</epage><pages>21259-21268</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Traditional Chinese medicine PaoTianXiong (PTX) is a processed product of
Debx. with polysaccharide as the main ingredient. The properties of PTX polysaccharide (PTXP) may be affected by different extraction methods. To develop and utilize PTXP better, it is of great significance to study the extraction methods of PTXP. Thus, we extracted PTXPs with dilute alkaline water extraction, ultrasound-assisted extraction, cellulase-assisted extraction, and hot water extraction (HWE), respectively. The characterizations of PTXPs extracted by different methods were analyzed based on purity determination, infrared analysis, molecular weight and monosaccharide composition. And antioxidant experiments of PTXPs were conducted. The results showed that PTXPs extracted by the four extraction methods were all glucan. After purification, the PTXPs showed similar antioxidant activity
. The molecular weight of polysaccharides extracted by the cellulase-assisted method was different from that extracted by the other three methods. Our results showed that not only the yield but also the effect of extraction methods on the properties of PTXP should be considered when selecting the best extraction method. Therefore, HWE was considered to be the best extraction method of PTXP. The yield and purity of purified PTXP were 24.5% and 97.1%, respectively. The optimized extraction conditions were: an extraction temperature of 90 °C, extraction time of 2.17 h, solid-liquid ratio of 1 : 29 (g mL
), and number of extractions of 2.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35478822</pmid><doi>10.1039/d1ra03628a</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1165-634X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Antioxidants Cellulase Chemistry Glucan Infrared analysis Molecular weight Monosaccharides Polysaccharides Purity Traditional Chinese medicine |
| title | Study on extraction methods of polysaccharides from a processed product of Aconitum carmichaeli Debx |
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