Effects of Treadmill Exercise on Mitochondrial DNA Damage and Cardiomyocyte Telomerase Activity in Aging Model Rats Based on Classical Apoptosis Signaling Pathway
In order to explore the effect of treadmill exercise on mitochondrial DNA damage and myocardial telomerase activity in aging model rats based on the classical apoptosis signaling pathway, a total of 36 clean-grade male SD rats are selected. After modeling, the rats are randomly divided into groups,...
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| description | In order to explore the effect of treadmill exercise on mitochondrial DNA damage and myocardial telomerase activity in aging model rats based on the classical apoptosis signaling pathway, a total of 36 clean-grade male SD rats are selected. After modeling, the rats are randomly divided into groups, namely, control and 3 times/w and 6 times/w exercise rats, with 12 rats in each group. After the rats of each group are modeled, the myocardial tissue and cells are collected, the apoptosis of myocardial cells is detected by TUNEL method, and the protein expressions of Bax and Bcl-2 in myocardial tissue are detected by western blotting. The mtDNA content of the control rats is the highest, which is significantly higher than that of the exercise group (P |
| doi_str_mv | 10.1155/2022/3529499 |
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After modeling, the rats are randomly divided into groups, namely, control and 3 times/w and 6 times/w exercise rats, with 12 rats in each group. After the rats of each group are modeled, the myocardial tissue and cells are collected, the apoptosis of myocardial cells is detected by TUNEL method, and the protein expressions of Bax and Bcl-2 in myocardial tissue are detected by western blotting. The mtDNA content of the control rats is the highest, which is significantly higher than that of the exercise group (P<0.05); the expression of mtDNA content in the heart of the rats exercising 3 times/w is significantly higher than that of the rats exercising 6 times/w (P<0.05); cardiomyocyte apoptosis AI value, Bcl-2, and Bax expressions of the control rats is the highest and significantly higher than those in the exercise group (P<0.05); Bcl-2/Bax in the control rats is the lowest and is significantly lower than that in the exercise group (P<0.05). The AI value, Bcl-2, and Bax expression of myocardial cell apoptosis in 3 times/w exercise rats are significantly higher than those in 6 times/w exercise rats (P<0.05); Bcl-2/Bax of 3 times/w exercise rats is significantly lower than that in 6 times/w exercise rats (P<0.05); by observing the rats that completed treadmill exercise, Akt2 protein of 3 times/w exercise rats and 6 times/w exercise rats is observed and analyzed. Compared with the control rats, the expressions of the two proteins are increased in 3 times/w exercise rats and 6 times/w exercise rats, and the upregulation in 6 times/w exercise rats is significantly increased and higher than that in 3 times/w exercise rats (P<0.05). For aging rats, treadmill exercise can reduce the body Bcl-2 and Bax values, improve the mitochondrial DNA damage and myocardial cell telomerase activity in aging model rats, and slow down the aging process.]]></description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2022/3529499</identifier><identifier>PMID: 35463973</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Aging ; Aging - genetics ; Aging - metabolism ; AKT2 protein ; Animals ; Antibodies ; Apoptosis ; BAX protein ; Bcl-2 protein ; bcl-2-Associated X Protein - genetics ; bcl-2-Associated X Protein - metabolism ; Cardiomyocytes ; Damage ; Data analysis ; DNA damage ; DNA, Mitochondrial - genetics ; DNA, Mitochondrial - metabolism ; Exercise ; Exercise Therapy - methods ; Fitness equipment ; Health aspects ; Heart ; Heart cells ; Male ; Mitochondrial DNA ; Myocytes, Cardiac - metabolism ; Physiological aspects ; Physiological research ; Proteins ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; Signaling ; Telomerase ; Telomerase - genetics ; Telomerase - metabolism ; Treadmill exercise tests ; Treadmills ; Western blotting</subject><ispartof>BioMed research international, 2022, Vol.2022 (1), p.3529499-3529499</ispartof><rights>Copyright © 2022 Chao Liang et al.</rights><rights>COPYRIGHT 2022 John Wiley & Sons, Inc.</rights><rights>Copyright © 2022 Chao Liang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Chao Liang et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-d832c609ee853c9f45136e34bb7ca8c1d0a508578904f96a060539d1b9dfff913</citedby><cites>FETCH-LOGICAL-c476t-d832c609ee853c9f45136e34bb7ca8c1d0a508578904f96a060539d1b9dfff913</cites><orcidid>0000-0002-2213-5681</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023140/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023140/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,4011,27905,27906,27907,53773,53775</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35463973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tang, Min</contributor><contributor>Min Tang</contributor><creatorcontrib>Liang, Chao</creatorcontrib><creatorcontrib>Zhou, Xiaoli</creatorcontrib><creatorcontrib>Li, Meiling</creatorcontrib><creatorcontrib>Song, Zhengpeng</creatorcontrib><creatorcontrib>Lan, Jinyan</creatorcontrib><title>Effects of Treadmill Exercise on Mitochondrial DNA Damage and Cardiomyocyte Telomerase Activity in Aging Model Rats Based on Classical Apoptosis Signaling Pathway</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description><![CDATA[In order to explore the effect of treadmill exercise on mitochondrial DNA damage and myocardial telomerase activity in aging model rats based on the classical apoptosis signaling pathway, a total of 36 clean-grade male SD rats are selected. After modeling, the rats are randomly divided into groups, namely, control and 3 times/w and 6 times/w exercise rats, with 12 rats in each group. After the rats of each group are modeled, the myocardial tissue and cells are collected, the apoptosis of myocardial cells is detected by TUNEL method, and the protein expressions of Bax and Bcl-2 in myocardial tissue are detected by western blotting. The mtDNA content of the control rats is the highest, which is significantly higher than that of the exercise group (P<0.05); the expression of mtDNA content in the heart of the rats exercising 3 times/w is significantly higher than that of the rats exercising 6 times/w (P<0.05); cardiomyocyte apoptosis AI value, Bcl-2, and Bax expressions of the control rats is the highest and significantly higher than those in the exercise group (P<0.05); Bcl-2/Bax in the control rats is the lowest and is significantly lower than that in the exercise group (P<0.05). The AI value, Bcl-2, and Bax expression of myocardial cell apoptosis in 3 times/w exercise rats are significantly higher than those in 6 times/w exercise rats (P<0.05); Bcl-2/Bax of 3 times/w exercise rats is significantly lower than that in 6 times/w exercise rats (P<0.05); by observing the rats that completed treadmill exercise, Akt2 protein of 3 times/w exercise rats and 6 times/w exercise rats is observed and analyzed. Compared with the control rats, the expressions of the two proteins are increased in 3 times/w exercise rats and 6 times/w exercise rats, and the upregulation in 6 times/w exercise rats is significantly increased and higher than that in 3 times/w exercise rats (P<0.05). For aging rats, treadmill exercise can reduce the body Bcl-2 and Bax values, improve the mitochondrial DNA damage and myocardial cell telomerase activity in aging model rats, and slow down the aging process.]]></description><subject>Aging</subject><subject>Aging - genetics</subject><subject>Aging - metabolism</subject><subject>AKT2 protein</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Apoptosis</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Cardiomyocytes</subject><subject>Damage</subject><subject>Data analysis</subject><subject>DNA damage</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Mitochondrial - metabolism</subject><subject>Exercise</subject><subject>Exercise Therapy - methods</subject><subject>Fitness equipment</subject><subject>Health aspects</subject><subject>Heart</subject><subject>Heart cells</subject><subject>Male</subject><subject>Mitochondrial DNA</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Physiological aspects</subject><subject>Physiological research</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>Telomerase</subject><subject>Telomerase - genetics</subject><subject>Telomerase - metabolism</subject><subject>Treadmill exercise tests</subject><subject>Treadmills</subject><subject>Western blotting</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kktvEzEURkcIRKvSHWtkiQ0ShNpjz2S8QRrSFJBaQBDW1o0fE1ceO7UnLfk7_aV4SAiPBfbClnx8rq_1FcVTgl8TUlVnJS7LM1qVnHH-oDguKWGTmjDy8LCn9Kg4Teka59GQGvP6cXFEK1ZTPqXHxf3cGC2HhIJBi6hB9dY5NP-uo7RJo-DRlR2CXAWvogWHzj-26Bx66DQCr9AMorKh3wa5HTRaaBd6HSFfbOVgb-2wRdajtrO-Q1dBaYe-QK71NhNqdM8cpGRl9rbrsB5Csgl9tZ0HN974DMPqDrZPikcGXNKn-_Wk-HYxX8zeTy4_vfsway8nkk3rYaIaWsrcn9ZNRSU3rCK01pQtl1MJjSQKQ4WbatpwzAyvAde4olyRJVfGGE7oSfFm511vlr1WUvshghPraHuIWxHAir9PvF2JLtwKjse_xlnwYi-I4Waj0yB6m6R2DrwOmyTKuqoIxoSxjD7_B70Om5j7_knRhufZ_KY6cFpYb0KuK0epaKfZ09Ql45l6taNkDClFbQ5PJliMKRFjSsQ-JRl_9mebB_hXJjLwcgesrFdwZ_-v-wGCNsS_</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Liang, Chao</creator><creator>Zhou, Xiaoli</creator><creator>Li, Meiling</creator><creator>Song, Zhengpeng</creator><creator>Lan, Jinyan</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2213-5681</orcidid></search><sort><creationdate>2022</creationdate><title>Effects of Treadmill Exercise on Mitochondrial DNA Damage and Cardiomyocyte Telomerase Activity in Aging Model Rats Based on Classical Apoptosis Signaling Pathway</title><author>Liang, Chao ; Zhou, Xiaoli ; Li, Meiling ; Song, Zhengpeng ; Lan, Jinyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-d832c609ee853c9f45136e34bb7ca8c1d0a508578904f96a060539d1b9dfff913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aging</topic><topic>Aging - genetics</topic><topic>Aging - metabolism</topic><topic>AKT2 protein</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Apoptosis</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>bcl-2-Associated X Protein - genetics</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Cardiomyocytes</topic><topic>Damage</topic><topic>Data analysis</topic><topic>DNA damage</topic><topic>DNA, Mitochondrial - genetics</topic><topic>DNA, Mitochondrial - metabolism</topic><topic>Exercise</topic><topic>Exercise Therapy - methods</topic><topic>Fitness equipment</topic><topic>Health aspects</topic><topic>Heart</topic><topic>Heart cells</topic><topic>Male</topic><topic>Mitochondrial DNA</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Physiological aspects</topic><topic>Physiological research</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Signal Transduction</topic><topic>Signaling</topic><topic>Telomerase</topic><topic>Telomerase - genetics</topic><topic>Telomerase - metabolism</topic><topic>Treadmill exercise tests</topic><topic>Treadmills</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Chao</creatorcontrib><creatorcontrib>Zhou, Xiaoli</creatorcontrib><creatorcontrib>Li, Meiling</creatorcontrib><creatorcontrib>Song, Zhengpeng</creatorcontrib><creatorcontrib>Lan, Jinyan</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Chao</au><au>Zhou, Xiaoli</au><au>Li, Meiling</au><au>Song, Zhengpeng</au><au>Lan, Jinyan</au><au>Tang, Min</au><au>Min Tang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Treadmill Exercise on Mitochondrial DNA Damage and Cardiomyocyte Telomerase Activity in Aging Model Rats Based on Classical Apoptosis Signaling Pathway</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2022</date><risdate>2022</risdate><volume>2022</volume><issue>1</issue><spage>3529499</spage><epage>3529499</epage><pages>3529499-3529499</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract><![CDATA[In order to explore the effect of treadmill exercise on mitochondrial DNA damage and myocardial telomerase activity in aging model rats based on the classical apoptosis signaling pathway, a total of 36 clean-grade male SD rats are selected. After modeling, the rats are randomly divided into groups, namely, control and 3 times/w and 6 times/w exercise rats, with 12 rats in each group. After the rats of each group are modeled, the myocardial tissue and cells are collected, the apoptosis of myocardial cells is detected by TUNEL method, and the protein expressions of Bax and Bcl-2 in myocardial tissue are detected by western blotting. The mtDNA content of the control rats is the highest, which is significantly higher than that of the exercise group (P<0.05); the expression of mtDNA content in the heart of the rats exercising 3 times/w is significantly higher than that of the rats exercising 6 times/w (P<0.05); cardiomyocyte apoptosis AI value, Bcl-2, and Bax expressions of the control rats is the highest and significantly higher than those in the exercise group (P<0.05); Bcl-2/Bax in the control rats is the lowest and is significantly lower than that in the exercise group (P<0.05). The AI value, Bcl-2, and Bax expression of myocardial cell apoptosis in 3 times/w exercise rats are significantly higher than those in 6 times/w exercise rats (P<0.05); Bcl-2/Bax of 3 times/w exercise rats is significantly lower than that in 6 times/w exercise rats (P<0.05); by observing the rats that completed treadmill exercise, Akt2 protein of 3 times/w exercise rats and 6 times/w exercise rats is observed and analyzed. Compared with the control rats, the expressions of the two proteins are increased in 3 times/w exercise rats and 6 times/w exercise rats, and the upregulation in 6 times/w exercise rats is significantly increased and higher than that in 3 times/w exercise rats (P<0.05). For aging rats, treadmill exercise can reduce the body Bcl-2 and Bax values, improve the mitochondrial DNA damage and myocardial cell telomerase activity in aging model rats, and slow down the aging process.]]></abstract><cop>United States</cop><pub>Hindawi</pub><pmid>35463973</pmid><doi>10.1155/2022/3529499</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2213-5681</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aging Aging - genetics Aging - metabolism AKT2 protein Animals Antibodies Apoptosis BAX protein Bcl-2 protein bcl-2-Associated X Protein - genetics bcl-2-Associated X Protein - metabolism Cardiomyocytes Damage Data analysis DNA damage DNA, Mitochondrial - genetics DNA, Mitochondrial - metabolism Exercise Exercise Therapy - methods Fitness equipment Health aspects Heart Heart cells Male Mitochondrial DNA Myocytes, Cardiac - metabolism Physiological aspects Physiological research Proteins Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Rats Rats, Sprague-Dawley Signal Transduction Signaling Telomerase Telomerase - genetics Telomerase - metabolism Treadmill exercise tests Treadmills Western blotting |
| title | Effects of Treadmill Exercise on Mitochondrial DNA Damage and Cardiomyocyte Telomerase Activity in Aging Model Rats Based on Classical Apoptosis Signaling Pathway |
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