Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae
Lav is an autotransporter protein found in pathogenic Haemophilus and species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA simple DNA sequence repeat tract. The expression status of was examined...
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| Veröffentlicht in: | Infection and immunity 2022-04, Vol.90 (4), p.e0056521-e0056521 |
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| creator | Phillips, Zachary N Garai, Preeti Tram, Greg Martin, Gael Van Den Bergh, Annelies Husna, Asma-Ul Staples, Megan Grimwood, Keith Jennison, Amy V Guillon, Patrice von Itzstein, Mark Jennings, Michael P Brockman, Kenneth L Atack, John M |
| description | Lav is an autotransporter protein found in pathogenic Haemophilus and
species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA
simple DNA sequence repeat tract. The expression status of
was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed
expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed
is present in ∼60% of NTHi strains, but
is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies. |
| doi_str_mv | 10.1128/iai.00565-21 |
| format | Article |
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species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA
simple DNA sequence repeat tract. The expression status of
was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed
expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed
is present in ∼60% of NTHi strains, but
is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/iai.00565-21</identifier><identifier>PMID: 35258316</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Biofilms ; Haemophilus Infections ; Haemophilus influenzae - genetics ; Haemophilus influenzae - metabolism ; Humans ; Microbial Pathogenesis ; Molecular Pathogenesis ; Type V Secretion Systems - genetics ; Type V Secretion Systems - metabolism</subject><ispartof>Infection and immunity, 2022-04, Vol.90 (4), p.e0056521-e0056521</ispartof><rights>Copyright © 2022 Phillips et al.</rights><rights>Copyright © 2022 Phillips et al. 2022 Phillips et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a3331-2f92a87d409d81d289f0e7cc3a7d552067ef3adc4a592bcbfb5901e79154b4713</citedby><cites>FETCH-LOGICAL-a3331-2f92a87d409d81d289f0e7cc3a7d552067ef3adc4a592bcbfb5901e79154b4713</cites><orcidid>0000-0002-1027-4684 ; 0000-0001-6302-7524 ; 0000-0002-7994-6995 ; 0000-0002-5599-7480</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.asm.org/doi/pdf/10.1128/iai.00565-21$$EPDF$$P50$$Gasm2$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.asm.org/doi/full/10.1128/iai.00565-21$$EHTML$$P50$$Gasm2$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,52751,52752,52753,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35258316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Monack, Denise</contributor><creatorcontrib>Phillips, Zachary N</creatorcontrib><creatorcontrib>Garai, Preeti</creatorcontrib><creatorcontrib>Tram, Greg</creatorcontrib><creatorcontrib>Martin, Gael</creatorcontrib><creatorcontrib>Van Den Bergh, Annelies</creatorcontrib><creatorcontrib>Husna, Asma-Ul</creatorcontrib><creatorcontrib>Staples, Megan</creatorcontrib><creatorcontrib>Grimwood, Keith</creatorcontrib><creatorcontrib>Jennison, Amy V</creatorcontrib><creatorcontrib>Guillon, Patrice</creatorcontrib><creatorcontrib>von Itzstein, Mark</creatorcontrib><creatorcontrib>Jennings, Michael P</creatorcontrib><creatorcontrib>Brockman, Kenneth L</creatorcontrib><creatorcontrib>Atack, John M</creatorcontrib><title>Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae</title><title>Infection and immunity</title><addtitle>Infect Immun</addtitle><addtitle>Infect Immun</addtitle><description>Lav is an autotransporter protein found in pathogenic Haemophilus and
species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA
simple DNA sequence repeat tract. The expression status of
was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed
expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed
is present in ∼60% of NTHi strains, but
is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies.</description><subject>Biofilms</subject><subject>Haemophilus Infections</subject><subject>Haemophilus influenzae - genetics</subject><subject>Haemophilus influenzae - metabolism</subject><subject>Humans</subject><subject>Microbial Pathogenesis</subject><subject>Molecular Pathogenesis</subject><subject>Type V Secretion Systems - genetics</subject><subject>Type V Secretion Systems - metabolism</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9v1DAQxS0Eokvhxhn5SCVS_CdO4gvSsmpZpFVBFXC1JsmEuErsYCcrtZ-FD4vbLVU5cLJG89N7nvcIec3ZKeeiem_BnjKmCpUJ_oSsONNVppQQT8mKMa4zrYryiLyI8SqNeZ5Xz8mRVEJVkhcr8nvTQ4BmxmBvYLbeUd_RuUf6tYeI2Q8IFuoB6XqZ_RzAxcmHBNMd7Okl7hGGSIFe-oRYR7c-znSDw0DXbY8BXYMUXEs_Wt_ZYaTnPowHlwRfeDdfT3gnvwUc_dTbYYlp1Q0LuhvAl-RZlwzw1f17TL6fn33bbLPdl0-fN-tdBlJKnolOC6jKNme6rXgrKt0xLJtGQtmmJFhRYiehbXJQWtRN3dVKM46l5iqv85LLY_LhoDst9Yhtgy6dOpgp2BHCtfFgzb8bZ3vz0--NZkKoUiSBt_cCwf9aMM5mtLFJOYBDv0QjCllKmZyqhL47oE3wMQbsHmw4M7eFmlSouSvUiNuvnRxwiKMwV34JLiXxP_bN4zMehP-2Lf8Aiv6snw</recordid><startdate>20220421</startdate><enddate>20220421</enddate><creator>Phillips, Zachary N</creator><creator>Garai, Preeti</creator><creator>Tram, Greg</creator><creator>Martin, Gael</creator><creator>Van Den Bergh, Annelies</creator><creator>Husna, Asma-Ul</creator><creator>Staples, Megan</creator><creator>Grimwood, Keith</creator><creator>Jennison, Amy V</creator><creator>Guillon, Patrice</creator><creator>von Itzstein, Mark</creator><creator>Jennings, Michael P</creator><creator>Brockman, Kenneth L</creator><creator>Atack, John M</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1027-4684</orcidid><orcidid>https://orcid.org/0000-0001-6302-7524</orcidid><orcidid>https://orcid.org/0000-0002-7994-6995</orcidid><orcidid>https://orcid.org/0000-0002-5599-7480</orcidid></search><sort><creationdate>20220421</creationdate><title>Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae</title><author>Phillips, Zachary N ; Garai, Preeti ; Tram, Greg ; Martin, Gael ; Van Den Bergh, Annelies ; Husna, Asma-Ul ; Staples, Megan ; Grimwood, Keith ; Jennison, Amy V ; Guillon, Patrice ; von Itzstein, Mark ; Jennings, Michael P ; Brockman, Kenneth L ; Atack, John M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a3331-2f92a87d409d81d289f0e7cc3a7d552067ef3adc4a592bcbfb5901e79154b4713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biofilms</topic><topic>Haemophilus Infections</topic><topic>Haemophilus influenzae - genetics</topic><topic>Haemophilus influenzae - metabolism</topic><topic>Humans</topic><topic>Microbial Pathogenesis</topic><topic>Molecular Pathogenesis</topic><topic>Type V Secretion Systems - genetics</topic><topic>Type V Secretion Systems - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phillips, Zachary N</creatorcontrib><creatorcontrib>Garai, Preeti</creatorcontrib><creatorcontrib>Tram, Greg</creatorcontrib><creatorcontrib>Martin, Gael</creatorcontrib><creatorcontrib>Van Den Bergh, Annelies</creatorcontrib><creatorcontrib>Husna, Asma-Ul</creatorcontrib><creatorcontrib>Staples, Megan</creatorcontrib><creatorcontrib>Grimwood, Keith</creatorcontrib><creatorcontrib>Jennison, Amy V</creatorcontrib><creatorcontrib>Guillon, Patrice</creatorcontrib><creatorcontrib>von Itzstein, Mark</creatorcontrib><creatorcontrib>Jennings, Michael P</creatorcontrib><creatorcontrib>Brockman, Kenneth L</creatorcontrib><creatorcontrib>Atack, John M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phillips, Zachary N</au><au>Garai, Preeti</au><au>Tram, Greg</au><au>Martin, Gael</au><au>Van Den Bergh, Annelies</au><au>Husna, Asma-Ul</au><au>Staples, Megan</au><au>Grimwood, Keith</au><au>Jennison, Amy V</au><au>Guillon, Patrice</au><au>von Itzstein, Mark</au><au>Jennings, Michael P</au><au>Brockman, Kenneth L</au><au>Atack, John M</au><au>Monack, Denise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae</atitle><jtitle>Infection and immunity</jtitle><stitle>Infect Immun</stitle><addtitle>Infect Immun</addtitle><date>2022-04-21</date><risdate>2022</risdate><volume>90</volume><issue>4</issue><spage>e0056521</spage><epage>e0056521</epage><pages>e0056521-e0056521</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><abstract>Lav is an autotransporter protein found in pathogenic Haemophilus and
species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA
simple DNA sequence repeat tract. The expression status of
was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed
expression (ON) results in increased adherence to human lung cells and denser biofilm formation. A survey of Haemophilus species genome sequences showed
is present in ∼60% of NTHi strains, but
is not present in most typeable H. influenzae strains. Sequence analysis revealed a total of five distinct variants of the Lav passenger domain present in Haemophilus spp., with these five variants showing a distinct lineage distribution. Determining the role of Lav in NTHi will help understand the role of this protein during distinct pathologies.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>35258316</pmid><doi>10.1128/iai.00565-21</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-1027-4684</orcidid><orcidid>https://orcid.org/0000-0001-6302-7524</orcidid><orcidid>https://orcid.org/0000-0002-7994-6995</orcidid><orcidid>https://orcid.org/0000-0002-5599-7480</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0019-9567 |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biofilms Haemophilus Infections Haemophilus influenzae - genetics Haemophilus influenzae - metabolism Humans Microbial Pathogenesis Molecular Pathogenesis Type V Secretion Systems - genetics Type V Secretion Systems - metabolism |
| title | Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae |
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