Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids

We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids from 160 patients with acute illness was evaluated using two sequencing platforms in comparison to microbiological testing...

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Veröffentlicht in:	Nature medicine 2021-01, Vol.27 (1), p.115-124
Hauptverfasser:	Gu, Wei, Deng, Xianding, Lee, Marco, Sucu, Yasemin D., Arevalo, Shaun, Stryke, Doug, Federman, Scot, Gopez, Allan, Reyes, Kevin, Zorn, Kelsey, Sample, Hannah, Yu, Guixia, Ishpuniani, Gurpreet, Briggs, Benjamin, Chow, Eric D., Berger, Amy, Wilson, Michael R., Wang, Candace, Hsu, Elaine, Miller, Steve, DeRisi, Joseph L., Chiu, Charles Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/326/2521 631/326/325 692/308/575 692/53/2421 692/699/255 Adult Aged Bacteria Bacteria - genetics Bacteria - isolation & purification Biomedical and Life Sciences Biomedicine Body fluids Body Fluids - microbiology Cancer Research Cell culture Cell-Free Nucleic Acids - analysis Cell-Free Nucleic Acids - genetics Computer applications Deoxyribonucleic acid Diagnosis DNA DNA sequencing Female Fungi Fungi - genetics Fungi - isolation & purification Genetic aspects Health aspects High-Throughput Nucleotide Sequencing - methods High-throughput screening (Biochemical assaying) Humans Identification and classification Infectious Diseases Male Metabolic Diseases Metagenomics Methods Middle Aged Molecular Medicine Neurosciences Next-generation sequencing Nucleotide sequencing Pathogenic microorganisms Pathogens Physiological aspects Polymerase chain reaction Porosity Sensitivity analysis
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