MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway
Accumulating evidence has indicated the crucial role of microRNA-196 in mediating tumor progression, while its significance in cholangiocarcinoma (CCA) remains unclear. In this study, we provided the first evidence that the expression level of miR-196-5p is elevated in both CCA cell lines and clinic...
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| Veröffentlicht in: | Journal of oncology 2022-04, Vol.2022, p.4599676-10 |
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| creator | Liu, Chao Li, Yanli Zhang, Lichao Zhang, Pei Yu, Ning Liu, Xuefang Lu, Huaihai Du, Haiming Hou, Senlin |
| description | Accumulating evidence has indicated the crucial role of microRNA-196 in mediating tumor progression, while its significance in cholangiocarcinoma (CCA) remains unclear. In this study, we provided the first evidence that the expression level of miR-196-5p is elevated in both CCA cell lines and clinic specimen. MiR-196-5p inhibition notably suppressed cell proliferation as well as metastasis in CCA cell line HuCCT1. Furthermore, the interaction between miR-196-5p and its downstream molecule HAND1 was verified. Moreover, a series of rescue assay verified that both HAND1 and β-catenin silencing could reverse the abnormal elevated cell proliferation and migration brought by miR-196-5p elevation, indicating that HAND1/Wnt/β-catenin signaling pathway activation is essential for miR-196-5p to exert its roles. In summary, we successfully depict the oncogenic role of miR-196-5p in promoting cell proliferation and migration in CCA via HAND1/Wnt/β-catenin axis. |
| doi_str_mv | 10.1155/2022/4599676 |
| format | Article |
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In this study, we provided the first evidence that the expression level of miR-196-5p is elevated in both CCA cell lines and clinic specimen. MiR-196-5p inhibition notably suppressed cell proliferation as well as metastasis in CCA cell line HuCCT1. Furthermore, the interaction between miR-196-5p and its downstream molecule HAND1 was verified. Moreover, a series of rescue assay verified that both HAND1 and β-catenin silencing could reverse the abnormal elevated cell proliferation and migration brought by miR-196-5p elevation, indicating that HAND1/Wnt/β-catenin signaling pathway activation is essential for miR-196-5p to exert its roles. In summary, we successfully depict the oncogenic role of miR-196-5p in promoting cell proliferation and migration in CCA via HAND1/Wnt/β-catenin axis.</description><identifier>ISSN: 1687-8450</identifier><identifier>EISSN: 1687-8450</identifier><identifier>DOI: 10.1155/2022/4599676</identifier><identifier>PMID: 35466323</identifier><language>eng</language><publisher>Egypt: Hindawi</publisher><subject>Bile ducts ; Cancer ; Cell cycle ; Cholangiocarcinoma ; Development and progression ; Experiments ; Gene expression ; Medical prognosis ; Medical research ; Metastasis ; Pathogenesis ; Plasmids ; Proteins ; Tumors</subject><ispartof>Journal of oncology, 2022-04, Vol.2022, p.4599676-10</ispartof><rights>Copyright © 2022 Chao Liu et al.</rights><rights>COPYRIGHT 2022 John Wiley & Sons, Inc.</rights><rights>Copyright © 2022 Chao Liu et al. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2022 Chao Liu et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-69359ec5dea100926dfe2ddf295f37d184eb94503b64cafbfa75de3e9405c313</citedby><cites>FETCH-LOGICAL-c476t-69359ec5dea100926dfe2ddf295f37d184eb94503b64cafbfa75de3e9405c313</cites><orcidid>0000-0003-1910-1824 ; 0000-0002-4776-6084 ; 0000-0002-7840-6989 ; 0000-0002-8678-6619 ; 0000-0003-2388-6054 ; 0000-0003-2830-7269 ; 0000-0003-1868-3391</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019430/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019430/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35466323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kang, Zhihua</contributor><contributor>Zhihua Kang</contributor><creatorcontrib>Liu, Chao</creatorcontrib><creatorcontrib>Li, Yanli</creatorcontrib><creatorcontrib>Zhang, Lichao</creatorcontrib><creatorcontrib>Zhang, Pei</creatorcontrib><creatorcontrib>Yu, Ning</creatorcontrib><creatorcontrib>Liu, Xuefang</creatorcontrib><creatorcontrib>Lu, Huaihai</creatorcontrib><creatorcontrib>Du, Haiming</creatorcontrib><creatorcontrib>Hou, Senlin</creatorcontrib><title>MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway</title><title>Journal of oncology</title><addtitle>J Oncol</addtitle><description>Accumulating evidence has indicated the crucial role of microRNA-196 in mediating tumor progression, while its significance in cholangiocarcinoma (CCA) remains unclear. In this study, we provided the first evidence that the expression level of miR-196-5p is elevated in both CCA cell lines and clinic specimen. MiR-196-5p inhibition notably suppressed cell proliferation as well as metastasis in CCA cell line HuCCT1. Furthermore, the interaction between miR-196-5p and its downstream molecule HAND1 was verified. Moreover, a series of rescue assay verified that both HAND1 and β-catenin silencing could reverse the abnormal elevated cell proliferation and migration brought by miR-196-5p elevation, indicating that HAND1/Wnt/β-catenin signaling pathway activation is essential for miR-196-5p to exert its roles. In summary, we successfully depict the oncogenic role of miR-196-5p in promoting cell proliferation and migration in CCA via HAND1/Wnt/β-catenin axis.</description><subject>Bile ducts</subject><subject>Cancer</subject><subject>Cell cycle</subject><subject>Cholangiocarcinoma</subject><subject>Development and progression</subject><subject>Experiments</subject><subject>Gene expression</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Metastasis</subject><subject>Pathogenesis</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Tumors</subject><issn>1687-8450</issn><issn>1687-8450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kctuEzEUhi0EoqWwY41GYoMEQ3yfeIMUhUuR2lJBJZbWiceeuJqxgz1p1dfiQXgmPCSUwoKVj-VPn8-vH6GnBL8mRIgZxZTOuFBKNvIeOiRy3tRzLvD9O_MBepTzJcaSYyUfogMmuJSMskOUTv3ns0VNlKzFpjpPcYijzdPQe2cTjD6GCkJbnfpuf_OhWq5jD6Hz0UAyPsQBqisP1fHi7C2ZfQ3j7Mf3egmjDYX94rsAvQ9ddQ7j-hpuHqMHDvpsn-zPI3Tx_t3F8rg--fTh43JxUhveyLGWiglljWgtEIwVla2ztG0dVcKxpiVzbleqZGMryQ24lYOmsMwqjoVhhB2hNzvtZrsabGtsGBP0epP8AOlGR_D675fg17qLV1phojjDRfBiL0jx29bmUQ8-G9uX5DZus6ZSCIKpVBP6_B_0Mm5Tif2LYgpTLOQfqoPeah9cLP-aSaoXDSZ8XoSsUK92lEkx52Td7coE66lxPTWu940X_NndmLfw74oL8HIHrH1o4dr_X_cTUTSysw</recordid><startdate>20220412</startdate><enddate>20220412</enddate><creator>Liu, Chao</creator><creator>Li, Yanli</creator><creator>Zhang, Lichao</creator><creator>Zhang, Pei</creator><creator>Yu, Ning</creator><creator>Liu, Xuefang</creator><creator>Lu, Huaihai</creator><creator>Du, Haiming</creator><creator>Hou, Senlin</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1910-1824</orcidid><orcidid>https://orcid.org/0000-0002-4776-6084</orcidid><orcidid>https://orcid.org/0000-0002-7840-6989</orcidid><orcidid>https://orcid.org/0000-0002-8678-6619</orcidid><orcidid>https://orcid.org/0000-0003-2388-6054</orcidid><orcidid>https://orcid.org/0000-0003-2830-7269</orcidid><orcidid>https://orcid.org/0000-0003-1868-3391</orcidid></search><sort><creationdate>20220412</creationdate><title>MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway</title><author>Liu, Chao ; Li, Yanli ; Zhang, Lichao ; Zhang, Pei ; Yu, Ning ; Liu, Xuefang ; Lu, Huaihai ; Du, Haiming ; Hou, Senlin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-69359ec5dea100926dfe2ddf295f37d184eb94503b64cafbfa75de3e9405c313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bile ducts</topic><topic>Cancer</topic><topic>Cell cycle</topic><topic>Cholangiocarcinoma</topic><topic>Development and progression</topic><topic>Experiments</topic><topic>Gene expression</topic><topic>Medical prognosis</topic><topic>Medical research</topic><topic>Metastasis</topic><topic>Pathogenesis</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Chao</creatorcontrib><creatorcontrib>Li, Yanli</creatorcontrib><creatorcontrib>Zhang, Lichao</creatorcontrib><creatorcontrib>Zhang, Pei</creatorcontrib><creatorcontrib>Yu, Ning</creatorcontrib><creatorcontrib>Liu, Xuefang</creatorcontrib><creatorcontrib>Lu, Huaihai</creatorcontrib><creatorcontrib>Du, Haiming</creatorcontrib><creatorcontrib>Hou, Senlin</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Chao</au><au>Li, Yanli</au><au>Zhang, Lichao</au><au>Zhang, Pei</au><au>Yu, Ning</au><au>Liu, Xuefang</au><au>Lu, Huaihai</au><au>Du, Haiming</au><au>Hou, Senlin</au><au>Kang, Zhihua</au><au>Zhihua Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway</atitle><jtitle>Journal of oncology</jtitle><addtitle>J Oncol</addtitle><date>2022-04-12</date><risdate>2022</risdate><volume>2022</volume><spage>4599676</spage><epage>10</epage><pages>4599676-10</pages><issn>1687-8450</issn><eissn>1687-8450</eissn><abstract>Accumulating evidence has indicated the crucial role of microRNA-196 in mediating tumor progression, while its significance in cholangiocarcinoma (CCA) remains unclear. In this study, we provided the first evidence that the expression level of miR-196-5p is elevated in both CCA cell lines and clinic specimen. MiR-196-5p inhibition notably suppressed cell proliferation as well as metastasis in CCA cell line HuCCT1. Furthermore, the interaction between miR-196-5p and its downstream molecule HAND1 was verified. Moreover, a series of rescue assay verified that both HAND1 and β-catenin silencing could reverse the abnormal elevated cell proliferation and migration brought by miR-196-5p elevation, indicating that HAND1/Wnt/β-catenin signaling pathway activation is essential for miR-196-5p to exert its roles. In summary, we successfully depict the oncogenic role of miR-196-5p in promoting cell proliferation and migration in CCA via HAND1/Wnt/β-catenin axis.</abstract><cop>Egypt</cop><pub>Hindawi</pub><pmid>35466323</pmid><doi>10.1155/2022/4599676</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1910-1824</orcidid><orcidid>https://orcid.org/0000-0002-4776-6084</orcidid><orcidid>https://orcid.org/0000-0002-7840-6989</orcidid><orcidid>https://orcid.org/0000-0002-8678-6619</orcidid><orcidid>https://orcid.org/0000-0003-2388-6054</orcidid><orcidid>https://orcid.org/0000-0003-2830-7269</orcidid><orcidid>https://orcid.org/0000-0003-1868-3391</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bile ducts Cancer Cell cycle Cholangiocarcinoma Development and progression Experiments Gene expression Medical prognosis Medical research Metastasis Pathogenesis Plasmids Proteins Tumors |
| title | MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway |
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