High-Mannose N -Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer
The increase incidence of early colorectal cancer (T1 CRC) last years is mainly due to the introduction of population-based screening for CRC. T1 CRC staging based on histological criteria remains challenging and there is high variability among pathologists in the scoring of these criteria. It is cr...
Gespeichert in:
| Veröffentlicht in: | Cancers 2022-03, Vol.14 (6), p.1552 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 6 |
| container_start_page | 1552 |
| container_title | Cancers |
| container_volume | 14 |
| creator | Boyaval, Fanny Dalebout, Hans Van Zeijl, René Wang, Wenjun Fariña-Sarasqueta, Arantza Lageveen-Kammeijer, Guinevere S M Boonstra, Jurjen J McDonnell, Liam A Wuhrer, Manfred Morreau, Hans Heijs, Bram |
| description | The increase incidence of early colorectal cancer (T1 CRC) last years is mainly due to the introduction of population-based screening for CRC. T1 CRC staging based on histological criteria remains challenging and there is high variability among pathologists in the scoring of these criteria. It is crucial to unravel the biology behind the progression of adenoma into T1 CRC. Glycomic studies have reported extensively on alterations of the
-glycomic pattern in CRC; therefore, investigating these alterations may reveal new insights into the development of T1 CRC. We used matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) to spatially profile the
-glycan species in a cohort of pT1 CRC using archival formalin-fixed and paraffin-embedded (FFPE) material. To generate structural information on the observed
-glycans, CE-ESI-MS/MS was used in conjunction with MALDI-MSI. Relative intensities and glycosylation traits were calculated based on a panel of 58
-glycans. Our analysis showed pronounced differences between normal epithelium, dysplastic, and carcinoma regions. High-mannose-type
-glycans were higher in the dysplastic region than in carcinoma, which correlates to increased proliferation of the cells. We observed changes in the cancer invasive front, including higher expression of α2,3-linked sialic acids which followed the glycosylation pattern of the carcinoma region. |
| doi_str_mv | 10.3390/cancers14061552 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8945895</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2642360267</sourcerecordid><originalsourceid>FETCH-LOGICAL-c487t-528ee4fb93caeb92f5f0bd91f48597ddc1be3ba0d10ad82e81647d1464fc1b8a3</originalsourceid><addsrcrecordid>eNpdkUtP3TAQhS3UChCw7g5ZYtNNil9xnA1SdUUBCWilwqIra-JMQsDXpnZupfvva54CvBnL883xzBxCvnD2TcqWHToIDlPmimle12KDbAvWiErrVn16c98ieznfsnKk5I1uNsmWrKXQDZPb5M_pNN5UFxBCzEgvaXXi10U3U8j0Avw0Bggz_ZXimDDnKYbymu7Kr3QK9BiSX1e_ZxiRLqKPCd0Mni4e-9olnwfwGfee4w65_nF8tTitzn-enC2-n1dOmWauamEQ1dC10gF2rRjqgXV9ywdl6rbpe8c7lB2wnjPojUDDtWp6rrQaSsqA3CFHT7r3q26JvcMwJ_D2Pk1LSGsbYbLvM2G6sWP8Z02ratPWReDrs0CKf1eYZ7ucskPvIWBcZSu0Uqxs07CCHnxAb-MqhTLeAyWkZmWthTp8olyKOSccXpvhzD44Zz84Vyr2387wyr_4JP8DFy6WPQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2642360267</pqid></control><display><type>article</type><title>High-Mannose N -Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Boyaval, Fanny ; Dalebout, Hans ; Van Zeijl, René ; Wang, Wenjun ; Fariña-Sarasqueta, Arantza ; Lageveen-Kammeijer, Guinevere S M ; Boonstra, Jurjen J ; McDonnell, Liam A ; Wuhrer, Manfred ; Morreau, Hans ; Heijs, Bram</creator><creatorcontrib>Boyaval, Fanny ; Dalebout, Hans ; Van Zeijl, René ; Wang, Wenjun ; Fariña-Sarasqueta, Arantza ; Lageveen-Kammeijer, Guinevere S M ; Boonstra, Jurjen J ; McDonnell, Liam A ; Wuhrer, Manfred ; Morreau, Hans ; Heijs, Bram</creatorcontrib><description>The increase incidence of early colorectal cancer (T1 CRC) last years is mainly due to the introduction of population-based screening for CRC. T1 CRC staging based on histological criteria remains challenging and there is high variability among pathologists in the scoring of these criteria. It is crucial to unravel the biology behind the progression of adenoma into T1 CRC. Glycomic studies have reported extensively on alterations of the
-glycomic pattern in CRC; therefore, investigating these alterations may reveal new insights into the development of T1 CRC. We used matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) to spatially profile the
-glycan species in a cohort of pT1 CRC using archival formalin-fixed and paraffin-embedded (FFPE) material. To generate structural information on the observed
-glycans, CE-ESI-MS/MS was used in conjunction with MALDI-MSI. Relative intensities and glycosylation traits were calculated based on a panel of 58
-glycans. Our analysis showed pronounced differences between normal epithelium, dysplastic, and carcinoma regions. High-mannose-type
-glycans were higher in the dysplastic region than in carcinoma, which correlates to increased proliferation of the cells. We observed changes in the cancer invasive front, including higher expression of α2,3-linked sialic acids which followed the glycosylation pattern of the carcinoma region.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers14061552</identifier><identifier>PMID: 35326703</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acids ; Adenoma ; Antibodies ; Cell proliferation ; Colorectal cancer ; Colorectal carcinoma ; Decision making ; Epithelium ; Exports ; Glycosylation ; Invasiveness ; Ions ; Lasers ; Lymphatic system ; Mannose ; Mass spectroscopy ; Metastases ; Metastasis ; Mutation ; N-glycans ; Paraffin ; Patients ; Polysaccharides ; Sialic acids ; Software ; Sugar ; Tumor microenvironment ; Tumors</subject><ispartof>Cancers, 2022-03, Vol.14 (6), p.1552</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-528ee4fb93caeb92f5f0bd91f48597ddc1be3ba0d10ad82e81647d1464fc1b8a3</citedby><cites>FETCH-LOGICAL-c487t-528ee4fb93caeb92f5f0bd91f48597ddc1be3ba0d10ad82e81647d1464fc1b8a3</cites><orcidid>0000-0002-4307-1568 ; 0000-0001-6328-9305 ; 0000-0001-7670-1151 ; 0000-0002-0645-1190 ; 0000-0002-0814-4995 ; 0000-0001-8898-0389</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945895/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945895/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35326703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boyaval, Fanny</creatorcontrib><creatorcontrib>Dalebout, Hans</creatorcontrib><creatorcontrib>Van Zeijl, René</creatorcontrib><creatorcontrib>Wang, Wenjun</creatorcontrib><creatorcontrib>Fariña-Sarasqueta, Arantza</creatorcontrib><creatorcontrib>Lageveen-Kammeijer, Guinevere S M</creatorcontrib><creatorcontrib>Boonstra, Jurjen J</creatorcontrib><creatorcontrib>McDonnell, Liam A</creatorcontrib><creatorcontrib>Wuhrer, Manfred</creatorcontrib><creatorcontrib>Morreau, Hans</creatorcontrib><creatorcontrib>Heijs, Bram</creatorcontrib><title>High-Mannose N -Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>The increase incidence of early colorectal cancer (T1 CRC) last years is mainly due to the introduction of population-based screening for CRC. T1 CRC staging based on histological criteria remains challenging and there is high variability among pathologists in the scoring of these criteria. It is crucial to unravel the biology behind the progression of adenoma into T1 CRC. Glycomic studies have reported extensively on alterations of the
-glycomic pattern in CRC; therefore, investigating these alterations may reveal new insights into the development of T1 CRC. We used matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) to spatially profile the
-glycan species in a cohort of pT1 CRC using archival formalin-fixed and paraffin-embedded (FFPE) material. To generate structural information on the observed
-glycans, CE-ESI-MS/MS was used in conjunction with MALDI-MSI. Relative intensities and glycosylation traits were calculated based on a panel of 58
-glycans. Our analysis showed pronounced differences between normal epithelium, dysplastic, and carcinoma regions. High-mannose-type
-glycans were higher in the dysplastic region than in carcinoma, which correlates to increased proliferation of the cells. We observed changes in the cancer invasive front, including higher expression of α2,3-linked sialic acids which followed the glycosylation pattern of the carcinoma region.</description><subject>Acids</subject><subject>Adenoma</subject><subject>Antibodies</subject><subject>Cell proliferation</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Decision making</subject><subject>Epithelium</subject><subject>Exports</subject><subject>Glycosylation</subject><subject>Invasiveness</subject><subject>Ions</subject><subject>Lasers</subject><subject>Lymphatic system</subject><subject>Mannose</subject><subject>Mass spectroscopy</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Mutation</subject><subject>N-glycans</subject><subject>Paraffin</subject><subject>Patients</subject><subject>Polysaccharides</subject><subject>Sialic acids</subject><subject>Software</subject><subject>Sugar</subject><subject>Tumor microenvironment</subject><subject>Tumors</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtP3TAQhS3UChCw7g5ZYtNNil9xnA1SdUUBCWilwqIra-JMQsDXpnZupfvva54CvBnL883xzBxCvnD2TcqWHToIDlPmimle12KDbAvWiErrVn16c98ieznfsnKk5I1uNsmWrKXQDZPb5M_pNN5UFxBCzEgvaXXi10U3U8j0Avw0Bggz_ZXimDDnKYbymu7Kr3QK9BiSX1e_ZxiRLqKPCd0Mni4e-9olnwfwGfee4w65_nF8tTitzn-enC2-n1dOmWauamEQ1dC10gF2rRjqgXV9ywdl6rbpe8c7lB2wnjPojUDDtWp6rrQaSsqA3CFHT7r3q26JvcMwJ_D2Pk1LSGsbYbLvM2G6sWP8Z02ratPWReDrs0CKf1eYZ7ucskPvIWBcZSu0Uqxs07CCHnxAb-MqhTLeAyWkZmWthTp8olyKOSccXpvhzD44Zz84Vyr2387wyr_4JP8DFy6WPQ</recordid><startdate>20220318</startdate><enddate>20220318</enddate><creator>Boyaval, Fanny</creator><creator>Dalebout, Hans</creator><creator>Van Zeijl, René</creator><creator>Wang, Wenjun</creator><creator>Fariña-Sarasqueta, Arantza</creator><creator>Lageveen-Kammeijer, Guinevere S M</creator><creator>Boonstra, Jurjen J</creator><creator>McDonnell, Liam A</creator><creator>Wuhrer, Manfred</creator><creator>Morreau, Hans</creator><creator>Heijs, Bram</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4307-1568</orcidid><orcidid>https://orcid.org/0000-0001-6328-9305</orcidid><orcidid>https://orcid.org/0000-0001-7670-1151</orcidid><orcidid>https://orcid.org/0000-0002-0645-1190</orcidid><orcidid>https://orcid.org/0000-0002-0814-4995</orcidid><orcidid>https://orcid.org/0000-0001-8898-0389</orcidid></search><sort><creationdate>20220318</creationdate><title>High-Mannose N -Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer</title><author>Boyaval, Fanny ; Dalebout, Hans ; Van Zeijl, René ; Wang, Wenjun ; Fariña-Sarasqueta, Arantza ; Lageveen-Kammeijer, Guinevere S M ; Boonstra, Jurjen J ; McDonnell, Liam A ; Wuhrer, Manfred ; Morreau, Hans ; Heijs, Bram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-528ee4fb93caeb92f5f0bd91f48597ddc1be3ba0d10ad82e81647d1464fc1b8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acids</topic><topic>Adenoma</topic><topic>Antibodies</topic><topic>Cell proliferation</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Decision making</topic><topic>Epithelium</topic><topic>Exports</topic><topic>Glycosylation</topic><topic>Invasiveness</topic><topic>Ions</topic><topic>Lasers</topic><topic>Lymphatic system</topic><topic>Mannose</topic><topic>Mass spectroscopy</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Mutation</topic><topic>N-glycans</topic><topic>Paraffin</topic><topic>Patients</topic><topic>Polysaccharides</topic><topic>Sialic acids</topic><topic>Software</topic><topic>Sugar</topic><topic>Tumor microenvironment</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boyaval, Fanny</creatorcontrib><creatorcontrib>Dalebout, Hans</creatorcontrib><creatorcontrib>Van Zeijl, René</creatorcontrib><creatorcontrib>Wang, Wenjun</creatorcontrib><creatorcontrib>Fariña-Sarasqueta, Arantza</creatorcontrib><creatorcontrib>Lageveen-Kammeijer, Guinevere S M</creatorcontrib><creatorcontrib>Boonstra, Jurjen J</creatorcontrib><creatorcontrib>McDonnell, Liam A</creatorcontrib><creatorcontrib>Wuhrer, Manfred</creatorcontrib><creatorcontrib>Morreau, Hans</creatorcontrib><creatorcontrib>Heijs, Bram</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boyaval, Fanny</au><au>Dalebout, Hans</au><au>Van Zeijl, René</au><au>Wang, Wenjun</au><au>Fariña-Sarasqueta, Arantza</au><au>Lageveen-Kammeijer, Guinevere S M</au><au>Boonstra, Jurjen J</au><au>McDonnell, Liam A</au><au>Wuhrer, Manfred</au><au>Morreau, Hans</au><au>Heijs, Bram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Mannose N -Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2022-03-18</date><risdate>2022</risdate><volume>14</volume><issue>6</issue><spage>1552</spage><pages>1552-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>The increase incidence of early colorectal cancer (T1 CRC) last years is mainly due to the introduction of population-based screening for CRC. T1 CRC staging based on histological criteria remains challenging and there is high variability among pathologists in the scoring of these criteria. It is crucial to unravel the biology behind the progression of adenoma into T1 CRC. Glycomic studies have reported extensively on alterations of the
-glycomic pattern in CRC; therefore, investigating these alterations may reveal new insights into the development of T1 CRC. We used matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) to spatially profile the
-glycan species in a cohort of pT1 CRC using archival formalin-fixed and paraffin-embedded (FFPE) material. To generate structural information on the observed
-glycans, CE-ESI-MS/MS was used in conjunction with MALDI-MSI. Relative intensities and glycosylation traits were calculated based on a panel of 58
-glycans. Our analysis showed pronounced differences between normal epithelium, dysplastic, and carcinoma regions. High-mannose-type
-glycans were higher in the dysplastic region than in carcinoma, which correlates to increased proliferation of the cells. We observed changes in the cancer invasive front, including higher expression of α2,3-linked sialic acids which followed the glycosylation pattern of the carcinoma region.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35326703</pmid><doi>10.3390/cancers14061552</doi><orcidid>https://orcid.org/0000-0002-4307-1568</orcidid><orcidid>https://orcid.org/0000-0001-6328-9305</orcidid><orcidid>https://orcid.org/0000-0001-7670-1151</orcidid><orcidid>https://orcid.org/0000-0002-0645-1190</orcidid><orcidid>https://orcid.org/0000-0002-0814-4995</orcidid><orcidid>https://orcid.org/0000-0001-8898-0389</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2072-6694 |
| ispartof | Cancers, 2022-03, Vol.14 (6), p.1552 |
| issn | 2072-6694 2072-6694 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8945895 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Acids Adenoma Antibodies Cell proliferation Colorectal cancer Colorectal carcinoma Decision making Epithelium Exports Glycosylation Invasiveness Ions Lasers Lymphatic system Mannose Mass spectroscopy Metastases Metastasis Mutation N-glycans Paraffin Patients Polysaccharides Sialic acids Software Sugar Tumor microenvironment Tumors |
| title | High-Mannose N -Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T06%3A00%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High-Mannose%20N%20-Glycans%20as%20Malignant%20Progression%20Markers%20in%20Early-Stage%20Colorectal%20Cancer&rft.jtitle=Cancers&rft.au=Boyaval,%20Fanny&rft.date=2022-03-18&rft.volume=14&rft.issue=6&rft.spage=1552&rft.pages=1552-&rft.issn=2072-6694&rft.eissn=2072-6694&rft_id=info:doi/10.3390/cancers14061552&rft_dat=%3Cproquest_pubme%3E2642360267%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2642360267&rft_id=info:pmid/35326703&rfr_iscdi=true |