Cytotoxic effects of resin-modified orthodontic band adhesives. Are they safe?
To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives. Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted...
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| Veröffentlicht in: | The Angle orthodontist 2010-09, Vol.80 (5), p.890-895 |
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| creator | Malkoc, Siddik Corekci, Bayram Botsali, Hayriye Esra Yalçin, Muhammet Sengun, Abdülkadir |
| description | To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives.
Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests.
Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P > .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group.
The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives. |
| doi_str_mv | 10.2319/101509-577.1 |
| format | Article |
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Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests.
Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P > .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group.
The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives.</description><identifier>ISSN: 0003-3219</identifier><identifier>EISSN: 1945-7103</identifier><identifier>DOI: 10.2319/101509-577.1</identifier><identifier>PMID: 20578860</identifier><language>eng</language><publisher>United States: Edward H Angle Education and Research Foundation, Inc</publisher><subject>Animals ; Biocompatible Materials - toxicity ; Bisphenol A-Glycidyl Methacrylate - chemistry ; Cell Count ; Cell Line ; Cell Shape - drug effects ; Cell Survival - drug effects ; Coloring Agents ; Composite Resins - chemistry ; Fibroblasts - drug effects ; Glass Ionomer Cements - chemistry ; Materials Testing ; Mice ; Mitochondria - drug effects ; Original ; Orthodontic Brackets ; Resin Cements - chemistry ; Resin Cements - toxicity ; Safety ; Succinate Dehydrogenase - drug effects ; Temperature ; Tetrazolium Salts ; Thiazoles ; Time Factors</subject><ispartof>The Angle orthodontist, 2010-09, Vol.80 (5), p.890-895</ispartof><rights>2010 by The EH Angle Education and Research Foundation, Inc. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c302t-ecea5ffa750db9f73aec42293ffb9bd339cf2f6356195722fe139d52ec0820723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939015/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939015/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20578860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malkoc, Siddik</creatorcontrib><creatorcontrib>Corekci, Bayram</creatorcontrib><creatorcontrib>Botsali, Hayriye Esra</creatorcontrib><creatorcontrib>Yalçin, Muhammet</creatorcontrib><creatorcontrib>Sengun, Abdülkadir</creatorcontrib><title>Cytotoxic effects of resin-modified orthodontic band adhesives. Are they safe?</title><title>The Angle orthodontist</title><addtitle>Angle Orthod</addtitle><description>To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives.
Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests.
Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P > .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group.
The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives.</description><subject>Animals</subject><subject>Biocompatible Materials - toxicity</subject><subject>Bisphenol A-Glycidyl Methacrylate - chemistry</subject><subject>Cell Count</subject><subject>Cell Line</subject><subject>Cell Shape - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Coloring Agents</subject><subject>Composite Resins - chemistry</subject><subject>Fibroblasts - drug effects</subject><subject>Glass Ionomer Cements - chemistry</subject><subject>Materials Testing</subject><subject>Mice</subject><subject>Mitochondria - drug effects</subject><subject>Original</subject><subject>Orthodontic Brackets</subject><subject>Resin Cements - chemistry</subject><subject>Resin Cements - toxicity</subject><subject>Safety</subject><subject>Succinate Dehydrogenase - drug effects</subject><subject>Temperature</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Time Factors</subject><issn>0003-3219</issn><issn>1945-7103</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1LAzEQQIMotlZvniU_wK2TpGk2F6UUv6DoRc8hm0zclXZTkrXYf-9KtehpDvPmDTxCzhmMuWD6igGToAup1JgdkCHTE1koBuKQDAFAFIIzPSAnOb8DcCkn_JgMOEhVllMYkqf5totd_GwcxRDQdZnGQBPmpi1W0TehQU9j6uroY9v1VGVbT62ve2KDeUxnCWlX45ZmG_DmlBwFu8x49jNH5PXu9mX-UCye7x_ns0XhBPCuQIdWhmCVBF_poIRFN-FcixAqXXkhtAs8TIWcMi0V5wGZ0F5ydFByUFyMyPXOu_6oVugdtl2yS7NOzcqmrYm2Mf83bVObt7gxpRa6D9YLLncCl2LOCcP-loH57mp2XU3f1bAev_j7bw__hhRfOO50kA</recordid><startdate>201009</startdate><enddate>201009</enddate><creator>Malkoc, Siddik</creator><creator>Corekci, Bayram</creator><creator>Botsali, Hayriye Esra</creator><creator>Yalçin, Muhammet</creator><creator>Sengun, Abdülkadir</creator><general>Edward H Angle Education and Research Foundation, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>201009</creationdate><title>Cytotoxic effects of resin-modified orthodontic band adhesives. Are they safe?</title><author>Malkoc, Siddik ; Corekci, Bayram ; Botsali, Hayriye Esra ; Yalçin, Muhammet ; Sengun, Abdülkadir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-ecea5ffa750db9f73aec42293ffb9bd339cf2f6356195722fe139d52ec0820723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biocompatible Materials - toxicity</topic><topic>Bisphenol A-Glycidyl Methacrylate - chemistry</topic><topic>Cell Count</topic><topic>Cell Line</topic><topic>Cell Shape - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Coloring Agents</topic><topic>Composite Resins - chemistry</topic><topic>Fibroblasts - drug effects</topic><topic>Glass Ionomer Cements - chemistry</topic><topic>Materials Testing</topic><topic>Mice</topic><topic>Mitochondria - drug effects</topic><topic>Original</topic><topic>Orthodontic Brackets</topic><topic>Resin Cements - chemistry</topic><topic>Resin Cements - toxicity</topic><topic>Safety</topic><topic>Succinate Dehydrogenase - drug effects</topic><topic>Temperature</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malkoc, Siddik</creatorcontrib><creatorcontrib>Corekci, Bayram</creatorcontrib><creatorcontrib>Botsali, Hayriye Esra</creatorcontrib><creatorcontrib>Yalçin, Muhammet</creatorcontrib><creatorcontrib>Sengun, Abdülkadir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Angle orthodontist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malkoc, Siddik</au><au>Corekci, Bayram</au><au>Botsali, Hayriye Esra</au><au>Yalçin, Muhammet</au><au>Sengun, Abdülkadir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxic effects of resin-modified orthodontic band adhesives. Are they safe?</atitle><jtitle>The Angle orthodontist</jtitle><addtitle>Angle Orthod</addtitle><date>2010-09</date><risdate>2010</risdate><volume>80</volume><issue>5</issue><spage>890</spage><epage>895</epage><pages>890-895</pages><issn>0003-3219</issn><eissn>1945-7103</eissn><abstract>To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives.
Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests.
Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P > .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group.
The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives.</abstract><cop>United States</cop><pub>Edward H Angle Education and Research Foundation, Inc</pub><pmid>20578860</pmid><doi>10.2319/101509-577.1</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Biocompatible Materials - toxicity Bisphenol A-Glycidyl Methacrylate - chemistry Cell Count Cell Line Cell Shape - drug effects Cell Survival - drug effects Coloring Agents Composite Resins - chemistry Fibroblasts - drug effects Glass Ionomer Cements - chemistry Materials Testing Mice Mitochondria - drug effects Original Orthodontic Brackets Resin Cements - chemistry Resin Cements - toxicity Safety Succinate Dehydrogenase - drug effects Temperature Tetrazolium Salts Thiazoles Time Factors |
| title | Cytotoxic effects of resin-modified orthodontic band adhesives. Are they safe? |
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