Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78
As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for t...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2022-03, Vol.106 (5-6), p.2235-2248 |
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| creator | Lian, Jie Steinert, Georg de Vree, Jeroen Meijer, Sven Heryanto, Christa Bosma, Rouke Wijffels, René H. Barbosa, Maria J. Smidt, Hauke Sipkema, Detmer |
| description | As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for the production of
Nannochloropsis
sp. CCAP211/78 spanning four months of operation from July to October. Illumina sequencing of 16S rRNA gene amplicons demonstrated that a wide variety of bacteria were present in all reactor types, with predominance of
Bacteroidetes
and
Alphaproteobacteria
. Bacterial communities were significantly different between all reactor types (except between the horizontal tubular reactor and the vertical tubular reactor) and also between runs in each reactor. Bacteria common to the majority of samples included one member of the
Saprospiraceae
family and one of the
NS11-12_marine group
(both
Bacteroidetes
). Hierarchical clustering analysis revealed two phases during the cultivation period separated by a major shift in bacterial community composition in the horizontal tubular reactor, the vertical tubular reactor and the raceway pond with a strong decrease of the
Saprospiraceae
and
NS11-12_marine group
that initially dominated the bacterial communities. Furthermore, we observed a less consistent pattern of bacterial taxa appearing in different reactors and runs, most of which belonging to the classes
Deltaproteobacteria
and
Flavobacteriia
. In addition, canonical correspondence analysis showed that the bacterial community composition was significantly correlated with the nitrate concentration. This study contributes to our understanding of bacterial diversity and composition in different types of outdoor reactors exposed to a range of dynamic biotic and abiotic factors.
Key points
•
Reactor types had significantly different bacterial communities except HT and VT
•
The inoculum source and physiochemical factors together affect bacterial community
•
The bacterial family Saprospiraceae is positively correlated to microalgal growth |
| doi_str_mv | 10.1007/s00253-022-11815-3 |
| format | Article |
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Nannochloropsis
sp. CCAP211/78 spanning four months of operation from July to October. Illumina sequencing of 16S rRNA gene amplicons demonstrated that a wide variety of bacteria were present in all reactor types, with predominance of
Bacteroidetes
and
Alphaproteobacteria
. Bacterial communities were significantly different between all reactor types (except between the horizontal tubular reactor and the vertical tubular reactor) and also between runs in each reactor. Bacteria common to the majority of samples included one member of the
Saprospiraceae
family and one of the
NS11-12_marine group
(both
Bacteroidetes
). Hierarchical clustering analysis revealed two phases during the cultivation period separated by a major shift in bacterial community composition in the horizontal tubular reactor, the vertical tubular reactor and the raceway pond with a strong decrease of the
Saprospiraceae
and
NS11-12_marine group
that initially dominated the bacterial communities. Furthermore, we observed a less consistent pattern of bacterial taxa appearing in different reactors and runs, most of which belonging to the classes
Deltaproteobacteria
and
Flavobacteriia
. In addition, canonical correspondence analysis showed that the bacterial community composition was significantly correlated with the nitrate concentration. This study contributes to our understanding of bacterial diversity and composition in different types of outdoor reactors exposed to a range of dynamic biotic and abiotic factors.
Key points
•
Reactor types had significantly different bacterial communities except HT and VT
•
The inoculum source and physiochemical factors together affect bacterial community
•
The bacterial family Saprospiraceae is positively correlated to microalgal growth</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-022-11815-3</identifier><identifier>PMID: 35166894</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abiotic factors ; Algae ; Analysis ; Aquatic microorganisms ; Bacteria ; Bacteria - genetics ; Bacteroidetes ; Biological diversity ; Biomedical and Life Sciences ; Bioreactors ; Bioreactors - microbiology ; Biotechnology ; Cluster analysis ; Clustering ; Community composition ; Composition ; Environmental Biotechnology ; Genetic aspects ; Identification and classification ; Inoculum ; Life Sciences ; Methods ; Microalgae ; Microbial Genetics and Genomics ; Microbiology ; Nannochloropsis ; Photobioreactors ; Physiochemistry ; Reactors ; RNA sequencing ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Stramenopiles - genetics</subject><ispartof>Applied microbiology and biotechnology, 2022-03, Vol.106 (5-6), p.2235-2248</ispartof><rights>The Author(s) 2022</rights><rights>2022. The Author(s).</rights><rights>COPYRIGHT 2022 Springer</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-5985209876d937f70219ac32cd1cf9b2b41c12a6622e8e24fa6706349cf0bfc63</citedby><cites>FETCH-LOGICAL-c541t-5985209876d937f70219ac32cd1cf9b2b41c12a6622e8e24fa6706349cf0bfc63</cites><orcidid>0000-0002-3583-5193</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-022-11815-3$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-022-11815-3$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35166894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lian, Jie</creatorcontrib><creatorcontrib>Steinert, Georg</creatorcontrib><creatorcontrib>de Vree, Jeroen</creatorcontrib><creatorcontrib>Meijer, Sven</creatorcontrib><creatorcontrib>Heryanto, Christa</creatorcontrib><creatorcontrib>Bosma, Rouke</creatorcontrib><creatorcontrib>Wijffels, René H.</creatorcontrib><creatorcontrib>Barbosa, Maria J.</creatorcontrib><creatorcontrib>Smidt, Hauke</creatorcontrib><creatorcontrib>Sipkema, Detmer</creatorcontrib><title>Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for the production of
Nannochloropsis
sp. CCAP211/78 spanning four months of operation from July to October. Illumina sequencing of 16S rRNA gene amplicons demonstrated that a wide variety of bacteria were present in all reactor types, with predominance of
Bacteroidetes
and
Alphaproteobacteria
. Bacterial communities were significantly different between all reactor types (except between the horizontal tubular reactor and the vertical tubular reactor) and also between runs in each reactor. Bacteria common to the majority of samples included one member of the
Saprospiraceae
family and one of the
NS11-12_marine group
(both
Bacteroidetes
). Hierarchical clustering analysis revealed two phases during the cultivation period separated by a major shift in bacterial community composition in the horizontal tubular reactor, the vertical tubular reactor and the raceway pond with a strong decrease of the
Saprospiraceae
and
NS11-12_marine group
that initially dominated the bacterial communities. Furthermore, we observed a less consistent pattern of bacterial taxa appearing in different reactors and runs, most of which belonging to the classes
Deltaproteobacteria
and
Flavobacteriia
. In addition, canonical correspondence analysis showed that the bacterial community composition was significantly correlated with the nitrate concentration. This study contributes to our understanding of bacterial diversity and composition in different types of outdoor reactors exposed to a range of dynamic biotic and abiotic factors.
Key points
•
Reactor types had significantly different bacterial communities except HT and VT
•
The inoculum source and physiochemical factors together affect bacterial community
•
The bacterial family Saprospiraceae is positively correlated to microalgal growth</description><subject>Abiotic factors</subject><subject>Algae</subject><subject>Analysis</subject><subject>Aquatic microorganisms</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteroidetes</subject><subject>Biological diversity</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Bioreactors - microbiology</subject><subject>Biotechnology</subject><subject>Cluster analysis</subject><subject>Clustering</subject><subject>Community composition</subject><subject>Composition</subject><subject>Environmental Biotechnology</subject><subject>Genetic aspects</subject><subject>Identification and classification</subject><subject>Inoculum</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Microalgae</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Nannochloropsis</subject><subject>Photobioreactors</subject><subject>Physiochemistry</subject><subject>Reactors</subject><subject>RNA sequencing</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Stramenopiles - genetics</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UsGO0zAQjRCILQs_wAFZ4sIl3bGdOPEFqVS7gLQCDnC2XMduvUozwXZW6j_w0TjbsrAIIR8sz7z3Rm_8iuIlhSUFaC4iAKt5CYyVlLa0LvmjYkErzkoQtHpcLIA2ddnUsj0rnsV4A0BZK8TT4ozXVIhWVovixzttkg1e96TztzZEnw7ED_nhnA12SASn1CEGMvoeExl7nWvjDhNuPAab2bmXDqONpJuCH7ZkDNhNJnkcCDqSdpbsvQmo-60mn_QwoNn1GHCMPpI4Lsl6vfrCKL1o2ufFE6f7aF-c7vPi29Xl1_WH8vrz-4_r1XVp6oqmMhuqGci2EZ3kjWuAUakNZ6ajxskN21TUUKaFYMy2llVOiwYEr6RxsHFG8PPi7VF3nDZ725lsM-hejcHvdTgo1F497Ax-p7Z4q1rJoQWaBd6cBAJ-n2xMau-jsX1ejsUpKiaYBAGynme9_gt6g1MYsr2MqqAWwO8ET6it7q3yg8M818yiaiVkU7EG-Ky1_Acqn87mFeNgnc_1BwR2JOT9xxisu_dIQc0ZUscMqZwhdZchxTPp1Z_buaf8Ck0G8CMgjvOH2_Db0n9kfwKsJ9JZ</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Lian, Jie</creator><creator>Steinert, Georg</creator><creator>de Vree, Jeroen</creator><creator>Meijer, Sven</creator><creator>Heryanto, Christa</creator><creator>Bosma, Rouke</creator><creator>Wijffels, René H.</creator><creator>Barbosa, Maria J.</creator><creator>Smidt, Hauke</creator><creator>Sipkema, Detmer</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3583-5193</orcidid></search><sort><creationdate>20220301</creationdate><title>Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78</title><author>Lian, Jie ; Steinert, Georg ; de Vree, Jeroen ; Meijer, Sven ; Heryanto, Christa ; Bosma, Rouke ; Wijffels, René H. ; Barbosa, Maria J. ; Smidt, Hauke ; Sipkema, Detmer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-5985209876d937f70219ac32cd1cf9b2b41c12a6622e8e24fa6706349cf0bfc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abiotic factors</topic><topic>Algae</topic><topic>Analysis</topic><topic>Aquatic microorganisms</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bacteroidetes</topic><topic>Biological diversity</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Bioreactors - microbiology</topic><topic>Biotechnology</topic><topic>Cluster analysis</topic><topic>Clustering</topic><topic>Community composition</topic><topic>Composition</topic><topic>Environmental Biotechnology</topic><topic>Genetic aspects</topic><topic>Identification and classification</topic><topic>Inoculum</topic><topic>Life Sciences</topic><topic>Methods</topic><topic>Microalgae</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Nannochloropsis</topic><topic>Photobioreactors</topic><topic>Physiochemistry</topic><topic>Reactors</topic><topic>RNA sequencing</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Stramenopiles - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lian, Jie</creatorcontrib><creatorcontrib>Steinert, Georg</creatorcontrib><creatorcontrib>de Vree, Jeroen</creatorcontrib><creatorcontrib>Meijer, Sven</creatorcontrib><creatorcontrib>Heryanto, Christa</creatorcontrib><creatorcontrib>Bosma, Rouke</creatorcontrib><creatorcontrib>Wijffels, René H.</creatorcontrib><creatorcontrib>Barbosa, Maria J.</creatorcontrib><creatorcontrib>Smidt, Hauke</creatorcontrib><creatorcontrib>Sipkema, Detmer</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Business Premium Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lian, Jie</au><au>Steinert, Georg</au><au>de Vree, Jeroen</au><au>Meijer, Sven</au><au>Heryanto, Christa</au><au>Bosma, Rouke</au><au>Wijffels, René H.</au><au>Barbosa, Maria J.</au><au>Smidt, Hauke</au><au>Sipkema, Detmer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>106</volume><issue>5-6</issue><spage>2235</spage><epage>2248</epage><pages>2235-2248</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for the production of
Nannochloropsis
sp. CCAP211/78 spanning four months of operation from July to October. Illumina sequencing of 16S rRNA gene amplicons demonstrated that a wide variety of bacteria were present in all reactor types, with predominance of
Bacteroidetes
and
Alphaproteobacteria
. Bacterial communities were significantly different between all reactor types (except between the horizontal tubular reactor and the vertical tubular reactor) and also between runs in each reactor. Bacteria common to the majority of samples included one member of the
Saprospiraceae
family and one of the
NS11-12_marine group
(both
Bacteroidetes
). Hierarchical clustering analysis revealed two phases during the cultivation period separated by a major shift in bacterial community composition in the horizontal tubular reactor, the vertical tubular reactor and the raceway pond with a strong decrease of the
Saprospiraceae
and
NS11-12_marine group
that initially dominated the bacterial communities. Furthermore, we observed a less consistent pattern of bacterial taxa appearing in different reactors and runs, most of which belonging to the classes
Deltaproteobacteria
and
Flavobacteriia
. In addition, canonical correspondence analysis showed that the bacterial community composition was significantly correlated with the nitrate concentration. This study contributes to our understanding of bacterial diversity and composition in different types of outdoor reactors exposed to a range of dynamic biotic and abiotic factors.
Key points
•
Reactor types had significantly different bacterial communities except HT and VT
•
The inoculum source and physiochemical factors together affect bacterial community
•
The bacterial family Saprospiraceae is positively correlated to microalgal growth</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35166894</pmid><doi>10.1007/s00253-022-11815-3</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-3583-5193</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2022-03, Vol.106 (5-6), p.2235-2248 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8930801 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Abiotic factors Algae Analysis Aquatic microorganisms Bacteria Bacteria - genetics Bacteroidetes Biological diversity Biomedical and Life Sciences Bioreactors Bioreactors - microbiology Biotechnology Cluster analysis Clustering Community composition Composition Environmental Biotechnology Genetic aspects Identification and classification Inoculum Life Sciences Methods Microalgae Microbial Genetics and Genomics Microbiology Nannochloropsis Photobioreactors Physiochemistry Reactors RNA sequencing RNA, Ribosomal, 16S - genetics rRNA 16S Stramenopiles - genetics |
| title | Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T06%3A33%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20diversity%20in%20different%20outdoor%20pilot%20plant%20photobioreactor%20types%20during%20production%20of%20the%20microalga%20Nannochloropsis%20sp.%20CCAP211/78&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Lian,%20Jie&rft.date=2022-03-01&rft.volume=106&rft.issue=5-6&rft.spage=2235&rft.epage=2248&rft.pages=2235-2248&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-022-11815-3&rft_dat=%3Cgale_pubme%3EA697427036%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2640560301&rft_id=info:pmid/35166894&rft_galeid=A697427036&rfr_iscdi=true |