circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation
Previous studies have identified the regulatory roles of circular RNAs (circRNAs) in human cancers. However, the molecular mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain largely unknown. This study screens the expression profile of circRNAs in HCC and identifies circVAMP3 as a signi...
Gespeichert in:
| Veröffentlicht in: | Advanced science 2022-03, Vol.9 (8), p.e2103817-n/a |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 8 |
| container_start_page | e2103817 |
| container_title | Advanced science |
| container_volume | 9 |
| creator | Chen, Shuai Cao, Xiaofei Zhang, Jinyang Wu, Wanying Zhang, Bing Zhao, Fangqing |
| description | Previous studies have identified the regulatory roles of circular RNAs (circRNAs) in human cancers. However, the molecular mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain largely unknown. This study screens the expression profile of circRNAs in HCC and identifies circVAMP3 as a significantly downregulated circRNA in HCC tissues. HCC patients with low circVAMP3 expression present poor prognosis. circVAMP3 negatively regulates the proliferation and metastasis of HCC cells in vitro and in vivo by driving phase separation of CAPRIN1 and promoting stress granule formation in cells, which can downregulate the protein level of Myc proto‐oncogene protein by inhibiting c‐Myc translation. Furthermore, circVAMP3 is widely expressed in many human tissues and is downregulated in related cancers. Therefore, circVAMP3 is a potential prognostic indicator for HCC and may serve as a therapeutic target for HCC treatment.
A circular RNA, circVAMP3, is characterized here which is downregulated in hepatocellular carcinoma and other tumors. circVAMP3 interacts with CAPRIN1 and G3BP1 to trigger phase separation of CAPRIN1 and promote stress granule formation in cells by acting as a molecular scaffolder. Through stress granules, circVAMP3 exerts its tumor suppressor properties by inhibiting translation of c‐Myc. |
| doi_str_mv | 10.1002/advs.202103817 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8922094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2638894634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5744-f828ed0c6d55d25725726d46a1c3d47649f5557ab27e051cc79484105412de023</originalsourceid><addsrcrecordid>eNqFkc1qGzEURkVpaYKbbZdBkE03dvU70mwCxkkaQ9KYOs1WyJIcK4yliTTj4l0foc_YJ-lMnZokm4JA4urocK8-AD5iNMIIkc_abvKIIIIRlVi8AYcEl3JIJWNvn50PwFHODwghzKlgWL4HB5QjQSjnhyAZn8zd-HpG4VnyG5fhZDz7Nv2K4Wyls4NzV-ukGx8D1MHCaVj5hW8yvOzqTTSuqtpKJzjRnSjEtYaLLZy3dZ1czj7cQ_P756_rrYG3SYdc_RV9AO-Wusru6GkfgO8X57eTy-HVzZfpZHw1NFwwNlxKIp1FprCcW8JFvwrLCo0NtUwUrFxyzoVeEOEQx8aIkkmGEWeYWIcIHYDTnbduF2tnjQtN0pWqk1_rtFVRe_XyJviVuo8bJUtCUMk6wacnQYqPrcuNWvvcj6yDi21WpCCESdR_5QCcvEIfYptCN15HUSlLVtBeONpRJsWck1vum8FI9YmqPlG1T7R7cPx8hD3-L78OYDvgh6_c9j86NT67m1PeNfIHy2esfw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2638894634</pqid></control><display><type>article</type><title>circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation</title><source>MEDLINE</source><source>Directory of Open Access Journals</source><source>Wiley Online Library Journals</source><source>Wiley_OA刊</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Chen, Shuai ; Cao, Xiaofei ; Zhang, Jinyang ; Wu, Wanying ; Zhang, Bing ; Zhao, Fangqing</creator><creatorcontrib>Chen, Shuai ; Cao, Xiaofei ; Zhang, Jinyang ; Wu, Wanying ; Zhang, Bing ; Zhao, Fangqing</creatorcontrib><description>Previous studies have identified the regulatory roles of circular RNAs (circRNAs) in human cancers. However, the molecular mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain largely unknown. This study screens the expression profile of circRNAs in HCC and identifies circVAMP3 as a significantly downregulated circRNA in HCC tissues. HCC patients with low circVAMP3 expression present poor prognosis. circVAMP3 negatively regulates the proliferation and metastasis of HCC cells in vitro and in vivo by driving phase separation of CAPRIN1 and promoting stress granule formation in cells, which can downregulate the protein level of Myc proto‐oncogene protein by inhibiting c‐Myc translation. Furthermore, circVAMP3 is widely expressed in many human tissues and is downregulated in related cancers. Therefore, circVAMP3 is a potential prognostic indicator for HCC and may serve as a therapeutic target for HCC treatment.
A circular RNA, circVAMP3, is characterized here which is downregulated in hepatocellular carcinoma and other tumors. circVAMP3 interacts with CAPRIN1 and G3BP1 to trigger phase separation of CAPRIN1 and promote stress granule formation in cells by acting as a molecular scaffolder. Through stress granules, circVAMP3 exerts its tumor suppressor properties by inhibiting translation of c‐Myc.</description><identifier>ISSN: 2198-3844</identifier><identifier>EISSN: 2198-3844</identifier><identifier>DOI: 10.1002/advs.202103817</identifier><identifier>PMID: 35072355</identifier><language>eng</language><publisher>Germany: John Wiley & Sons, Inc</publisher><subject>CAPRIN1 ; Carcinoma, Hepatocellular - genetics ; Cell adhesion & migration ; Cell cycle ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; Cell growth ; circVAMP3 ; Gene Expression Regulation, Neoplastic - genetics ; Genes ; Genomes ; hepatocellular carcinoma ; Humans ; liquid–liquid phase separation ; Liver cancer ; Liver Neoplasms - genetics ; Medical prognosis ; Metastasis ; MicroRNAs ; MicroRNAs - genetics ; Polymerase chain reaction ; RNA, Circular - genetics ; Survival analysis ; Tumors</subject><ispartof>Advanced science, 2022-03, Vol.9 (8), p.e2103817-n/a</ispartof><rights>2022 The Authors. Advanced Science published by Wiley‐VCH GmbH</rights><rights>2022 The Authors. Advanced Science published by Wiley-VCH GmbH.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5744-f828ed0c6d55d25725726d46a1c3d47649f5557ab27e051cc79484105412de023</citedby><cites>FETCH-LOGICAL-c5744-f828ed0c6d55d25725726d46a1c3d47649f5557ab27e051cc79484105412de023</cites><orcidid>0000-0002-6216-1235</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922094/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8922094/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35072355$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Shuai</creatorcontrib><creatorcontrib>Cao, Xiaofei</creatorcontrib><creatorcontrib>Zhang, Jinyang</creatorcontrib><creatorcontrib>Wu, Wanying</creatorcontrib><creatorcontrib>Zhang, Bing</creatorcontrib><creatorcontrib>Zhao, Fangqing</creatorcontrib><title>circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation</title><title>Advanced science</title><addtitle>Adv Sci (Weinh)</addtitle><description>Previous studies have identified the regulatory roles of circular RNAs (circRNAs) in human cancers. However, the molecular mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain largely unknown. This study screens the expression profile of circRNAs in HCC and identifies circVAMP3 as a significantly downregulated circRNA in HCC tissues. HCC patients with low circVAMP3 expression present poor prognosis. circVAMP3 negatively regulates the proliferation and metastasis of HCC cells in vitro and in vivo by driving phase separation of CAPRIN1 and promoting stress granule formation in cells, which can downregulate the protein level of Myc proto‐oncogene protein by inhibiting c‐Myc translation. Furthermore, circVAMP3 is widely expressed in many human tissues and is downregulated in related cancers. Therefore, circVAMP3 is a potential prognostic indicator for HCC and may serve as a therapeutic target for HCC treatment.
A circular RNA, circVAMP3, is characterized here which is downregulated in hepatocellular carcinoma and other tumors. circVAMP3 interacts with CAPRIN1 and G3BP1 to trigger phase separation of CAPRIN1 and promote stress granule formation in cells by acting as a molecular scaffolder. Through stress granules, circVAMP3 exerts its tumor suppressor properties by inhibiting translation of c‐Myc.</description><subject>CAPRIN1</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Cell adhesion & migration</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell growth</subject><subject>circVAMP3</subject><subject>Gene Expression Regulation, Neoplastic - genetics</subject><subject>Genes</subject><subject>Genomes</subject><subject>hepatocellular carcinoma</subject><subject>Humans</subject><subject>liquid–liquid phase separation</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Polymerase chain reaction</subject><subject>RNA, Circular - genetics</subject><subject>Survival analysis</subject><subject>Tumors</subject><issn>2198-3844</issn><issn>2198-3844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc1qGzEURkVpaYKbbZdBkE03dvU70mwCxkkaQ9KYOs1WyJIcK4yliTTj4l0foc_YJ-lMnZokm4JA4urocK8-AD5iNMIIkc_abvKIIIIRlVi8AYcEl3JIJWNvn50PwFHODwghzKlgWL4HB5QjQSjnhyAZn8zd-HpG4VnyG5fhZDz7Nv2K4Wyls4NzV-ukGx8D1MHCaVj5hW8yvOzqTTSuqtpKJzjRnSjEtYaLLZy3dZ1czj7cQ_P756_rrYG3SYdc_RV9AO-Wusru6GkfgO8X57eTy-HVzZfpZHw1NFwwNlxKIp1FprCcW8JFvwrLCo0NtUwUrFxyzoVeEOEQx8aIkkmGEWeYWIcIHYDTnbduF2tnjQtN0pWqk1_rtFVRe_XyJviVuo8bJUtCUMk6wacnQYqPrcuNWvvcj6yDi21WpCCESdR_5QCcvEIfYptCN15HUSlLVtBeONpRJsWck1vum8FI9YmqPlG1T7R7cPx8hD3-L78OYDvgh6_c9j86NT67m1PeNfIHy2esfw</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Chen, Shuai</creator><creator>Cao, Xiaofei</creator><creator>Zhang, Jinyang</creator><creator>Wu, Wanying</creator><creator>Zhang, Bing</creator><creator>Zhao, Fangqing</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6216-1235</orcidid></search><sort><creationdate>20220301</creationdate><title>circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation</title><author>Chen, Shuai ; Cao, Xiaofei ; Zhang, Jinyang ; Wu, Wanying ; Zhang, Bing ; Zhao, Fangqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5744-f828ed0c6d55d25725726d46a1c3d47649f5557ab27e051cc79484105412de023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>CAPRIN1</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Cell adhesion & migration</topic><topic>Cell cycle</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell growth</topic><topic>circVAMP3</topic><topic>Gene Expression Regulation, Neoplastic - genetics</topic><topic>Genes</topic><topic>Genomes</topic><topic>hepatocellular carcinoma</topic><topic>Humans</topic><topic>liquid–liquid phase separation</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>Polymerase chain reaction</topic><topic>RNA, Circular - genetics</topic><topic>Survival analysis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shuai</creatorcontrib><creatorcontrib>Cao, Xiaofei</creatorcontrib><creatorcontrib>Zhang, Jinyang</creatorcontrib><creatorcontrib>Wu, Wanying</creatorcontrib><creatorcontrib>Zhang, Bing</creatorcontrib><creatorcontrib>Zhao, Fangqing</creatorcontrib><collection>Wiley_OA刊</collection><collection>Wiley Free Archive</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Academic</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest research library</collection><collection>ProQuest Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Advanced science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shuai</au><au>Cao, Xiaofei</au><au>Zhang, Jinyang</au><au>Wu, Wanying</au><au>Zhang, Bing</au><au>Zhao, Fangqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation</atitle><jtitle>Advanced science</jtitle><addtitle>Adv Sci (Weinh)</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>9</volume><issue>8</issue><spage>e2103817</spage><epage>n/a</epage><pages>e2103817-n/a</pages><issn>2198-3844</issn><eissn>2198-3844</eissn><abstract>Previous studies have identified the regulatory roles of circular RNAs (circRNAs) in human cancers. However, the molecular mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain largely unknown. This study screens the expression profile of circRNAs in HCC and identifies circVAMP3 as a significantly downregulated circRNA in HCC tissues. HCC patients with low circVAMP3 expression present poor prognosis. circVAMP3 negatively regulates the proliferation and metastasis of HCC cells in vitro and in vivo by driving phase separation of CAPRIN1 and promoting stress granule formation in cells, which can downregulate the protein level of Myc proto‐oncogene protein by inhibiting c‐Myc translation. Furthermore, circVAMP3 is widely expressed in many human tissues and is downregulated in related cancers. Therefore, circVAMP3 is a potential prognostic indicator for HCC and may serve as a therapeutic target for HCC treatment.
A circular RNA, circVAMP3, is characterized here which is downregulated in hepatocellular carcinoma and other tumors. circVAMP3 interacts with CAPRIN1 and G3BP1 to trigger phase separation of CAPRIN1 and promote stress granule formation in cells by acting as a molecular scaffolder. Through stress granules, circVAMP3 exerts its tumor suppressor properties by inhibiting translation of c‐Myc.</abstract><cop>Germany</cop><pub>John Wiley & Sons, Inc</pub><pmid>35072355</pmid><doi>10.1002/advs.202103817</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-6216-1235</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2198-3844 |
| ispartof | Advanced science, 2022-03, Vol.9 (8), p.e2103817-n/a |
| issn | 2198-3844 2198-3844 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8922094 |
| source | MEDLINE; Directory of Open Access Journals; Wiley Online Library Journals; Wiley_OA刊; PubMed Central; EZB Electronic Journals Library |
| subjects | CAPRIN1 Carcinoma, Hepatocellular - genetics Cell adhesion & migration Cell cycle Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Cell growth circVAMP3 Gene Expression Regulation, Neoplastic - genetics Genes Genomes hepatocellular carcinoma Humans liquid–liquid phase separation Liver cancer Liver Neoplasms - genetics Medical prognosis Metastasis MicroRNAs MicroRNAs - genetics Polymerase chain reaction RNA, Circular - genetics Survival analysis Tumors |
| title | circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T14%3A54%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=circVAMP3%20Drives%20CAPRIN1%20Phase%20Separation%20and%20Inhibits%20Hepatocellular%20Carcinoma%20by%20Suppressing%20c%E2%80%90Myc%20Translation&rft.jtitle=Advanced%20science&rft.au=Chen,%20Shuai&rft.date=2022-03-01&rft.volume=9&rft.issue=8&rft.spage=e2103817&rft.epage=n/a&rft.pages=e2103817-n/a&rft.issn=2198-3844&rft.eissn=2198-3844&rft_id=info:doi/10.1002/advs.202103817&rft_dat=%3Cproquest_pubme%3E2638894634%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2638894634&rft_id=info:pmid/35072355&rfr_iscdi=true |