Dietary carbohydrate modifies the density of L cells in the chicken ileum
Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate various physiological functions in the gastrointestinal tract. The secretion of GLPs is influenced by macronutrient ingestion. This study aims to clarify the effects of dietary carbohydrate (CHO) on L cells in the chick...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2022, Vol.84(2), pp.265-274 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 274 |
|---|---|
| container_issue | 2 |
| container_start_page | 265 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 84 |
| creator | SALAHUDDIN, Md HIRAMATSU, Kohzy NISHIMOTO, Iori KITA, Kazumi |
| description | Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate various physiological functions in the gastrointestinal tract. The secretion of GLPs is influenced by macronutrient ingestion. This study aims to clarify the effects of dietary carbohydrate (CHO) on L cells in the chicken ileum. Six-week-old, male White Leghorn chickens were divided into three groups: control, low-CHO and CHO-free, with five chickens in each group. Paraffin sections were made from the proximal and distal ileum of each animal and subjected to immunohistochemistry for GLP-1 and GLP-2 peptides and in situ hybridization for proglucagon (PG) mRNA. A significant reduction of GLP-1- and GLP-2-immunoreactive cells was observed in the two experimental groups compared with that in the control. A reduction of cells expressing PG mRNA was observed in the proximal and distal ileum of the CHO-free group compared with that in the control. The ratio of GLP-1-immunoreactive cells showing Ki-67 immunoreactivity was significantly lower in the distal ileum of the CHO-free group than that in the control group. These data suggest that dietary CHO is an effective stimulator for modifying L cell density in the chicken ileum. |
| doi_str_mv | 10.1292/jvms.21-0572 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8920715</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2616600412</sourcerecordid><originalsourceid>FETCH-LOGICAL-c618t-f107e6442a9556e0a78f49925c8e5b5efbc0783d1a05c09b52393909fbb39c623</originalsourceid><addsrcrecordid>eNpdkUtv1DAYRS0EokNhxxpZYsOCFL8db5BKeVUaiQ2sLcf50nhI4mI7lebfkzRlBGzsxT069tVF6CUlF5QZ9u5wN-YLRisiNXuEdpQLXWnBzWO0I4aqSjNJztCznA-EMCqUeYrOuDA10VLt0PXHAMWlI_YuNbE_tskVwGNsQxcg49IDbmHKoRxx7PAeexiGjMN0n_g--J8w4TDAPD5HTzo3ZHjxcJ-jH58_fb_6Wu2_fbm-utxXXtG6VB0lGpQQzBkpFRCn604Yw6SvQTYSusYTXfOWOiI9MY1k3HBDTNc03HjF-Dl6v3lv52aE1sNUkhvsbQrj0sNGF-y_yRR6exPvbG0Y0VQugjcPghR_zZCLHUNee7kJ4pwtU1QpQgRd33r9H3qIc5qWegvFRa0pZ3yh3m6UTzHnBN3pM5TYdSO7bmQZtetGC_7q7wIn-M8oC_BhAw65uBs4AS6V4AfYbLWw7P7YrKfQ9y5ZmPhvTEmk0g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2634871323</pqid></control><display><type>article</type><title>Dietary carbohydrate modifies the density of L cells in the chicken ileum</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><source>PubMed Central</source><creator>SALAHUDDIN, Md ; HIRAMATSU, Kohzy ; NISHIMOTO, Iori ; KITA, Kazumi</creator><creatorcontrib>SALAHUDDIN, Md ; HIRAMATSU, Kohzy ; NISHIMOTO, Iori ; KITA, Kazumi</creatorcontrib><description>Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate various physiological functions in the gastrointestinal tract. The secretion of GLPs is influenced by macronutrient ingestion. This study aims to clarify the effects of dietary carbohydrate (CHO) on L cells in the chicken ileum. Six-week-old, male White Leghorn chickens were divided into three groups: control, low-CHO and CHO-free, with five chickens in each group. Paraffin sections were made from the proximal and distal ileum of each animal and subjected to immunohistochemistry for GLP-1 and GLP-2 peptides and in situ hybridization for proglucagon (PG) mRNA. A significant reduction of GLP-1- and GLP-2-immunoreactive cells was observed in the two experimental groups compared with that in the control. A reduction of cells expressing PG mRNA was observed in the proximal and distal ileum of the CHO-free group compared with that in the control. The ratio of GLP-1-immunoreactive cells showing Ki-67 immunoreactivity was significantly lower in the distal ileum of the CHO-free group than that in the control group. These data suggest that dietary CHO is an effective stimulator for modifying L cell density in the chicken ileum.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.21-0572</identifier><identifier>PMID: 34980756</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Anatomy ; Animals ; carbohydrate ; Cell density ; chicken ; Chickens ; Dietary Carbohydrates - pharmacology ; Enteroendocrine Cells ; Gastrointestinal tract ; Glucagon ; glucagon-like peptides ; Hybridization ; Ileum ; Immunohistochemistry ; Ki-67 ; L Cells ; Male ; Mice ; mRNA ; Paraffin ; Peptides ; Poultry ; proglucagon mRNA ; Small intestine</subject><ispartof>Journal of Veterinary Medical Science, 2022, Vol.84(2), pp.265-274</ispartof><rights>2022 by the Japanese Society of Veterinary Science</rights><rights>2022. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 The Japanese Society of Veterinary Science 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-f107e6442a9556e0a78f49925c8e5b5efbc0783d1a05c09b52393909fbb39c623</citedby><cites>FETCH-LOGICAL-c618t-f107e6442a9556e0a78f49925c8e5b5efbc0783d1a05c09b52393909fbb39c623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920715/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920715/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,1884,4025,27928,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34980756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SALAHUDDIN, Md</creatorcontrib><creatorcontrib>HIRAMATSU, Kohzy</creatorcontrib><creatorcontrib>NISHIMOTO, Iori</creatorcontrib><creatorcontrib>KITA, Kazumi</creatorcontrib><title>Dietary carbohydrate modifies the density of L cells in the chicken ileum</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate various physiological functions in the gastrointestinal tract. The secretion of GLPs is influenced by macronutrient ingestion. This study aims to clarify the effects of dietary carbohydrate (CHO) on L cells in the chicken ileum. Six-week-old, male White Leghorn chickens were divided into three groups: control, low-CHO and CHO-free, with five chickens in each group. Paraffin sections were made from the proximal and distal ileum of each animal and subjected to immunohistochemistry for GLP-1 and GLP-2 peptides and in situ hybridization for proglucagon (PG) mRNA. A significant reduction of GLP-1- and GLP-2-immunoreactive cells was observed in the two experimental groups compared with that in the control. A reduction of cells expressing PG mRNA was observed in the proximal and distal ileum of the CHO-free group compared with that in the control. The ratio of GLP-1-immunoreactive cells showing Ki-67 immunoreactivity was significantly lower in the distal ileum of the CHO-free group than that in the control group. These data suggest that dietary CHO is an effective stimulator for modifying L cell density in the chicken ileum.</description><subject>Anatomy</subject><subject>Animals</subject><subject>carbohydrate</subject><subject>Cell density</subject><subject>chicken</subject><subject>Chickens</subject><subject>Dietary Carbohydrates - pharmacology</subject><subject>Enteroendocrine Cells</subject><subject>Gastrointestinal tract</subject><subject>Glucagon</subject><subject>glucagon-like peptides</subject><subject>Hybridization</subject><subject>Ileum</subject><subject>Immunohistochemistry</subject><subject>Ki-67</subject><subject>L Cells</subject><subject>Male</subject><subject>Mice</subject><subject>mRNA</subject><subject>Paraffin</subject><subject>Peptides</subject><subject>Poultry</subject><subject>proglucagon mRNA</subject><subject>Small intestine</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtv1DAYRS0EokNhxxpZYsOCFL8db5BKeVUaiQ2sLcf50nhI4mI7lebfkzRlBGzsxT069tVF6CUlF5QZ9u5wN-YLRisiNXuEdpQLXWnBzWO0I4aqSjNJztCznA-EMCqUeYrOuDA10VLt0PXHAMWlI_YuNbE_tskVwGNsQxcg49IDbmHKoRxx7PAeexiGjMN0n_g--J8w4TDAPD5HTzo3ZHjxcJ-jH58_fb_6Wu2_fbm-utxXXtG6VB0lGpQQzBkpFRCn604Yw6SvQTYSusYTXfOWOiI9MY1k3HBDTNc03HjF-Dl6v3lv52aE1sNUkhvsbQrj0sNGF-y_yRR6exPvbG0Y0VQugjcPghR_zZCLHUNee7kJ4pwtU1QpQgRd33r9H3qIc5qWegvFRa0pZ3yh3m6UTzHnBN3pM5TYdSO7bmQZtetGC_7q7wIn-M8oC_BhAw65uBs4AS6V4AfYbLWw7P7YrKfQ9y5ZmPhvTEmk0g</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>SALAHUDDIN, Md</creator><creator>HIRAMATSU, Kohzy</creator><creator>NISHIMOTO, Iori</creator><creator>KITA, Kazumi</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2022</creationdate><title>Dietary carbohydrate modifies the density of L cells in the chicken ileum</title><author>SALAHUDDIN, Md ; HIRAMATSU, Kohzy ; NISHIMOTO, Iori ; KITA, Kazumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-f107e6442a9556e0a78f49925c8e5b5efbc0783d1a05c09b52393909fbb39c623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Anatomy</topic><topic>Animals</topic><topic>carbohydrate</topic><topic>Cell density</topic><topic>chicken</topic><topic>Chickens</topic><topic>Dietary Carbohydrates - pharmacology</topic><topic>Enteroendocrine Cells</topic><topic>Gastrointestinal tract</topic><topic>Glucagon</topic><topic>glucagon-like peptides</topic><topic>Hybridization</topic><topic>Ileum</topic><topic>Immunohistochemistry</topic><topic>Ki-67</topic><topic>L Cells</topic><topic>Male</topic><topic>Mice</topic><topic>mRNA</topic><topic>Paraffin</topic><topic>Peptides</topic><topic>Poultry</topic><topic>proglucagon mRNA</topic><topic>Small intestine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SALAHUDDIN, Md</creatorcontrib><creatorcontrib>HIRAMATSU, Kohzy</creatorcontrib><creatorcontrib>NISHIMOTO, Iori</creatorcontrib><creatorcontrib>KITA, Kazumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SALAHUDDIN, Md</au><au>HIRAMATSU, Kohzy</au><au>NISHIMOTO, Iori</au><au>KITA, Kazumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary carbohydrate modifies the density of L cells in the chicken ileum</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2022</date><risdate>2022</risdate><volume>84</volume><issue>2</issue><spage>265</spage><epage>274</epage><pages>265-274</pages><artnum>21-0572</artnum><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate various physiological functions in the gastrointestinal tract. The secretion of GLPs is influenced by macronutrient ingestion. This study aims to clarify the effects of dietary carbohydrate (CHO) on L cells in the chicken ileum. Six-week-old, male White Leghorn chickens were divided into three groups: control, low-CHO and CHO-free, with five chickens in each group. Paraffin sections were made from the proximal and distal ileum of each animal and subjected to immunohistochemistry for GLP-1 and GLP-2 peptides and in situ hybridization for proglucagon (PG) mRNA. A significant reduction of GLP-1- and GLP-2-immunoreactive cells was observed in the two experimental groups compared with that in the control. A reduction of cells expressing PG mRNA was observed in the proximal and distal ileum of the CHO-free group compared with that in the control. The ratio of GLP-1-immunoreactive cells showing Ki-67 immunoreactivity was significantly lower in the distal ileum of the CHO-free group than that in the control group. These data suggest that dietary CHO is an effective stimulator for modifying L cell density in the chicken ileum.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>34980756</pmid><doi>10.1292/jvms.21-0572</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2022, Vol.84(2), pp.265-274 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8920715 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; PubMed Central |
| subjects | Anatomy Animals carbohydrate Cell density chicken Chickens Dietary Carbohydrates - pharmacology Enteroendocrine Cells Gastrointestinal tract Glucagon glucagon-like peptides Hybridization Ileum Immunohistochemistry Ki-67 L Cells Male Mice mRNA Paraffin Peptides Poultry proglucagon mRNA Small intestine |
| title | Dietary carbohydrate modifies the density of L cells in the chicken ileum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T01%3A55%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dietary%20carbohydrate%20modifies%20the%20density%20of%20L%20cells%20in%20the%20chicken%20ileum&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=SALAHUDDIN,%20Md&rft.date=2022&rft.volume=84&rft.issue=2&rft.spage=265&rft.epage=274&rft.pages=265-274&rft.artnum=21-0572&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.21-0572&rft_dat=%3Cproquest_pubme%3E2616600412%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2634871323&rft_id=info:pmid/34980756&rfr_iscdi=true |