miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR
Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR-150-5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. Th...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2022-04, Vol.23 (4), p.283, Article 283 |
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| description | Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR-150-5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. The expression of miR-150-5p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcription-quantitative (RT-q)PCR and western blotting. Following overexpression of miR-150-5p, the alteration in osteogenic gene expression was detected by RT-qPCR, western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR-150-5p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR-150-5p in joint capsules. Treatment with bone morphogenic protein 2 (BMP-2) and transforming growth factor-β1 (TGF-β1) led to downregulation of miR-150-5p in AS fibroblasts. Enforced expression of miR-150-5p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR-150-5p inhibited osteogenic differentiation of AS fibroblasts by targeting VDR. miR-150-5p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS. |
| doi_str_mv | 10.3892/etm.2022.11213 |
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The aim of the present study was to investigate the potential function of miR-150-5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. The expression of miR-150-5p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcription-quantitative (RT-q)PCR and western blotting. Following overexpression of miR-150-5p, the alteration in osteogenic gene expression was detected by RT-qPCR, western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR-150-5p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR-150-5p in joint capsules. Treatment with bone morphogenic protein 2 (BMP-2) and transforming growth factor-β1 (TGF-β1) led to downregulation of miR-150-5p in AS fibroblasts. Enforced expression of miR-150-5p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR-150-5p inhibited osteogenic differentiation of AS fibroblasts by targeting VDR. miR-150-5p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2022.11213</identifier><identifier>PMID: 35317439</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Ankylosing spondylitis ; Antibodies ; Arthritis ; Bioinformatics ; Causes of ; Cell differentiation ; Development and progression ; Fibroblasts ; Fractures ; Gene expression ; Genetic aspects ; Health aspects ; Ligaments ; Medical research ; Medicine, Experimental ; MicroRNA ; MicroRNAs ; Osteoblasts ; Proteins</subject><ispartof>Experimental and therapeutic medicine, 2022-04, Vol.23 (4), p.283, Article 283</ispartof><rights>Copyright: © Li et al.</rights><rights>COPYRIGHT 2022 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2022</rights><rights>Copyright: © Li et al. 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-1635fa914b08230331bbe2f2648212d331fbd3917e6356c4a669389ca6258a853</citedby><cites>FETCH-LOGICAL-c415t-1635fa914b08230331bbe2f2648212d331fbd3917e6356c4a669389ca6258a853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908459/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908459/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35317439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Qi, Wufang</creatorcontrib><creatorcontrib>Shi, Yuquan</creatorcontrib><title>miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR-150-5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. The expression of miR-150-5p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcription-quantitative (RT-q)PCR and western blotting. Following overexpression of miR-150-5p, the alteration in osteogenic gene expression was detected by RT-qPCR, western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR-150-5p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR-150-5p in joint capsules. Treatment with bone morphogenic protein 2 (BMP-2) and transforming growth factor-β1 (TGF-β1) led to downregulation of miR-150-5p in AS fibroblasts. Enforced expression of miR-150-5p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR-150-5p inhibited osteogenic differentiation of AS fibroblasts by targeting VDR. miR-150-5p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS.</description><subject>Ankylosing spondylitis</subject><subject>Antibodies</subject><subject>Arthritis</subject><subject>Bioinformatics</subject><subject>Causes of</subject><subject>Cell differentiation</subject><subject>Development and progression</subject><subject>Fibroblasts</subject><subject>Fractures</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Ligaments</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Osteoblasts</subject><subject>Proteins</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkd1rFDEUxYMottS--igDPs-am0xmkheh1E8oCEV9DZlMMnvrTLImWWH_e7O6lgpNHvJxf_dwkkPIS6AbLhV748q6YZSxDQAD_oScw6BYCxTE09OeKgln5DLnO1qH6EFK8ZycccFh6Lg6J9sVb1sQtBW7BsMWRyy5ibm4OLuAtpnQe5dcKGgKxtBE33gcUxwXkyuJoTHhx2GJGcPc5F0M02HBgrkZD00xaXblWPj-7vYFeebNkt3lab0g3z68_3r9qb358vHz9dVNazsQpYWeC28UdCOVjFPOYRwd86zvJAM21bMfJ65gcBXsbWf6XtW_sKZnQhop-AV5-1d3tx9XN9lqPZlF7xKuJh10NKj_rwTc6jn-0lJR2QlVBV6fBFL8uXe56Lu4T6F61tVFN_TV4gNqNovTGHysYnbFbPXVQKligkqo1OYRqs7JrWhjcB7r_WMNNsWck_P3xoHqY-a6Zq6Pmes_mdeGVw-fe4__S5j_Bu_Spks</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Li, Yuan</creator><creator>Qi, Wufang</creator><creator>Shi, Yuquan</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20220401</creationdate><title>miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR</title><author>Li, Yuan ; Qi, Wufang ; Shi, Yuquan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-1635fa914b08230331bbe2f2648212d331fbd3917e6356c4a669389ca6258a853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Ankylosing spondylitis</topic><topic>Antibodies</topic><topic>Arthritis</topic><topic>Bioinformatics</topic><topic>Causes of</topic><topic>Cell differentiation</topic><topic>Development and progression</topic><topic>Fibroblasts</topic><topic>Fractures</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Ligaments</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>Osteoblasts</topic><topic>Proteins</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Qi, Wufang</creatorcontrib><creatorcontrib>Shi, Yuquan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuan</au><au>Qi, Wufang</au><au>Shi, Yuquan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2022-04-01</date><risdate>2022</risdate><volume>23</volume><issue>4</issue><spage>283</spage><pages>283-</pages><artnum>283</artnum><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR-150-5p in osteogenic differentiation of AS fibroblasts and its underlying mechanism. The expression of miR-150-5p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcription-quantitative (RT-q)PCR and western blotting. Following overexpression of miR-150-5p, the alteration in osteogenic gene expression was detected by RT-qPCR, western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR-150-5p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR-150-5p in joint capsules. Treatment with bone morphogenic protein 2 (BMP-2) and transforming growth factor-β1 (TGF-β1) led to downregulation of miR-150-5p in AS fibroblasts. Enforced expression of miR-150-5p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR-150-5p inhibited osteogenic differentiation of AS fibroblasts by targeting VDR. miR-150-5p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>35317439</pmid><doi>10.3892/etm.2022.11213</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Ankylosing spondylitis Antibodies Arthritis Bioinformatics Causes of Cell differentiation Development and progression Fibroblasts Fractures Gene expression Genetic aspects Health aspects Ligaments Medical research Medicine, Experimental MicroRNA MicroRNAs Osteoblasts Proteins |
| title | miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR |
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