Detecting Schistosoma haematobium infection by microscopy and polymerase chain reaction (PCR) in school children in three senatorial districts of Cross River State, Nigeria
As a result of the poor sensitivity and specificity of the standard parasitological diagnostic methods currently being used, this study was conducted to compare the standard parasitological diagnostic methods and Polymerase Chain Reaction (PCR) in determining the prevalence of urinary schistosomiasi...
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| Veröffentlicht in: | Journal of parasitic diseases 2022-03, Vol.46 (1), p.272-279 |
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| creator | Esiere, Rosemary Kaiso Ibeneme, Emmanuel Onyekachukwu Effanga, Emmanuel Offiong Imalele, Edema Enogiomwan Esiere, Miracle Kaiso Inyang-Etoh, Paul Columba Alaribe, Ambrose Andrew Anyanwu |
| description | As a result of the poor sensitivity and specificity of the standard parasitological diagnostic methods currently being used, this study was conducted to compare the standard parasitological diagnostic methods and Polymerase Chain Reaction (PCR) in determining the prevalence of urinary schistosomiasis in Cross River State (CRS). The study was conducted between April 2015 and March 2016. Seven hundred and seventy seven (777) urine samples were randomly collected from selected school-age children. The urine samples were subjected to standard parasitological and molecular examinations. Chi-square test was used to test the differences between the data on subgroups and the results from specimen examinations. An overall prevalence of 1.7% was recorded using microscopy and 34.7% recorded using PCR. The highest prevalence of infection by microscopy occurred in the Southern Senatorial District (2.3%), while the Northern Senatorial District recorded the highest prevalence of infection by PCR (53.2%) (
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| doi_str_mv | 10.1007/s12639-021-01446-2 |
| format | Article |
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p
< 0.05). Males were more infected (2.4%) than females (0.6%) using microscopy. With PCR, males were also more infected (35.7%) compared to females (33.3%) (
p
< 0.05). The highest prevalence of infection using microscopy and PCR both occurred in school-age children aged 5–8 years (3.6% and 47.8% respectively), while the lowest prevalence for both methods occurred in participants aged 17 – 20 years (0% for both methods) (
p
< 0.05). This study has shown PCR to be effective in detecting schistosomiasis infection and also re-affirms the endemicity of urinary schistosomiasis in the three Senatorial Districts of CRS.</description><identifier>ISSN: 0971-7196</identifier><identifier>EISSN: 0975-0703</identifier><identifier>DOI: 10.1007/s12639-021-01446-2</identifier><identifier>PMID: 35299919</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Children ; Health Promotion and Disease Prevention ; Infections ; Infectious Diseases ; Medicine ; Medicine & Public Health ; Microscopy ; Original ; Original Article ; Polymerase chain reaction ; Schistosomiasis</subject><ispartof>Journal of parasitic diseases, 2022-03, Vol.46 (1), p.272-279</ispartof><rights>Indian Society for Parasitology 2021</rights><rights>Indian Society for Parasitology 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3892-10e9304ca9248e4b74e65e2b3e137a8cde55630851a4cfe93099dfebcf606d313</citedby><cites>FETCH-LOGICAL-c3892-10e9304ca9248e4b74e65e2b3e137a8cde55630851a4cfe93099dfebcf606d313</cites><orcidid>0000-0003-3774-5121</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901918/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901918/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,41495,42564,51326,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35299919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Esiere, Rosemary Kaiso</creatorcontrib><creatorcontrib>Ibeneme, Emmanuel Onyekachukwu</creatorcontrib><creatorcontrib>Effanga, Emmanuel Offiong</creatorcontrib><creatorcontrib>Imalele, Edema Enogiomwan</creatorcontrib><creatorcontrib>Esiere, Miracle Kaiso</creatorcontrib><creatorcontrib>Inyang-Etoh, Paul Columba</creatorcontrib><creatorcontrib>Alaribe, Ambrose Andrew Anyanwu</creatorcontrib><title>Detecting Schistosoma haematobium infection by microscopy and polymerase chain reaction (PCR) in school children in three senatorial districts of Cross River State, Nigeria</title><title>Journal of parasitic diseases</title><addtitle>J Parasit Dis</addtitle><addtitle>J Parasit Dis</addtitle><description>As a result of the poor sensitivity and specificity of the standard parasitological diagnostic methods currently being used, this study was conducted to compare the standard parasitological diagnostic methods and Polymerase Chain Reaction (PCR) in determining the prevalence of urinary schistosomiasis in Cross River State (CRS). The study was conducted between April 2015 and March 2016. Seven hundred and seventy seven (777) urine samples were randomly collected from selected school-age children. The urine samples were subjected to standard parasitological and molecular examinations. Chi-square test was used to test the differences between the data on subgroups and the results from specimen examinations. An overall prevalence of 1.7% was recorded using microscopy and 34.7% recorded using PCR. The highest prevalence of infection by microscopy occurred in the Southern Senatorial District (2.3%), while the Northern Senatorial District recorded the highest prevalence of infection by PCR (53.2%) (
p
< 0.05). Males were more infected (2.4%) than females (0.6%) using microscopy. With PCR, males were also more infected (35.7%) compared to females (33.3%) (
p
< 0.05). The highest prevalence of infection using microscopy and PCR both occurred in school-age children aged 5–8 years (3.6% and 47.8% respectively), while the lowest prevalence for both methods occurred in participants aged 17 – 20 years (0% for both methods) (
p
< 0.05). This study has shown PCR to be effective in detecting schistosomiasis infection and also re-affirms the endemicity of urinary schistosomiasis in the three Senatorial Districts of CRS.</description><subject>Children</subject><subject>Health Promotion and Disease Prevention</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy</subject><subject>Original</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Schistosomiasis</subject><issn>0971-7196</issn><issn>0975-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kstu1TAQhiMEoqXwAiyQJTZFIuBbLt4gocNVqgC1sLYcZ3LiKrEPtlPpvBMPyaSnlMuClS3PN_PPjP-ieMzoC0Zp8zIxXgtVUs5KyqSsS36nOKaqqUraUHH3-s7Khqn6qHiQ0iWlFb6394sjUXGlFFPHxY83kMFm57fkwo4u5ZDCbMhoYDY5dG6ZifPDSgRPuj2ZnY0h2bDbE-N7sgvTfoZoEhA7GudJBHNgT79szp9hLkl2DGHCsJv6CH59ymMEIAk8SkRnJtKjcHQ2JxIGskGBRM7dFURykU2G5-ST2wKCD4t7g5kSPLo5T4pv795-3Xwozz6__7h5fVZa0SpeMgpKUGmN4rIF2TUS6gp4J4CJxrS2h6qqBW0rZqQdVlapfoDODjWte8HESfHqUHe3dDP0FnyOZtK76GYT9zoYp_-OeDfqbbjSraJMsRYLnN4UiOH7Ainr2SUL02Q8hCVpXkvUbCspEX36D3oZluhxPKREUzXYqUKKH6h1-ynCcNsMo3o1gz6YQaMZ9LUZNMekJ3-OcZvy6_cREAcgYcjjin9r_6fsT5oSw5s</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Esiere, Rosemary Kaiso</creator><creator>Ibeneme, Emmanuel Onyekachukwu</creator><creator>Effanga, Emmanuel Offiong</creator><creator>Imalele, Edema Enogiomwan</creator><creator>Esiere, Miracle Kaiso</creator><creator>Inyang-Etoh, Paul Columba</creator><creator>Alaribe, Ambrose Andrew Anyanwu</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3774-5121</orcidid></search><sort><creationdate>20220301</creationdate><title>Detecting Schistosoma haematobium infection by microscopy and polymerase chain reaction (PCR) in school children in three senatorial districts of Cross River State, Nigeria</title><author>Esiere, Rosemary Kaiso ; Ibeneme, Emmanuel Onyekachukwu ; Effanga, Emmanuel Offiong ; Imalele, Edema Enogiomwan ; Esiere, Miracle Kaiso ; Inyang-Etoh, Paul Columba ; Alaribe, Ambrose Andrew Anyanwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3892-10e9304ca9248e4b74e65e2b3e137a8cde55630851a4cfe93099dfebcf606d313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Children</topic><topic>Health Promotion and Disease Prevention</topic><topic>Infections</topic><topic>Infectious Diseases</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy</topic><topic>Original</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Schistosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Esiere, Rosemary Kaiso</creatorcontrib><creatorcontrib>Ibeneme, Emmanuel Onyekachukwu</creatorcontrib><creatorcontrib>Effanga, Emmanuel Offiong</creatorcontrib><creatorcontrib>Imalele, Edema Enogiomwan</creatorcontrib><creatorcontrib>Esiere, Miracle Kaiso</creatorcontrib><creatorcontrib>Inyang-Etoh, Paul Columba</creatorcontrib><creatorcontrib>Alaribe, Ambrose Andrew Anyanwu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of parasitic diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Esiere, Rosemary Kaiso</au><au>Ibeneme, Emmanuel Onyekachukwu</au><au>Effanga, Emmanuel Offiong</au><au>Imalele, Edema Enogiomwan</au><au>Esiere, Miracle Kaiso</au><au>Inyang-Etoh, Paul Columba</au><au>Alaribe, Ambrose Andrew Anyanwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detecting Schistosoma haematobium infection by microscopy and polymerase chain reaction (PCR) in school children in three senatorial districts of Cross River State, Nigeria</atitle><jtitle>Journal of parasitic diseases</jtitle><stitle>J Parasit Dis</stitle><addtitle>J Parasit Dis</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>46</volume><issue>1</issue><spage>272</spage><epage>279</epage><pages>272-279</pages><issn>0971-7196</issn><eissn>0975-0703</eissn><abstract>As a result of the poor sensitivity and specificity of the standard parasitological diagnostic methods currently being used, this study was conducted to compare the standard parasitological diagnostic methods and Polymerase Chain Reaction (PCR) in determining the prevalence of urinary schistosomiasis in Cross River State (CRS). The study was conducted between April 2015 and March 2016. Seven hundred and seventy seven (777) urine samples were randomly collected from selected school-age children. The urine samples were subjected to standard parasitological and molecular examinations. Chi-square test was used to test the differences between the data on subgroups and the results from specimen examinations. An overall prevalence of 1.7% was recorded using microscopy and 34.7% recorded using PCR. The highest prevalence of infection by microscopy occurred in the Southern Senatorial District (2.3%), while the Northern Senatorial District recorded the highest prevalence of infection by PCR (53.2%) (
p
< 0.05). Males were more infected (2.4%) than females (0.6%) using microscopy. With PCR, males were also more infected (35.7%) compared to females (33.3%) (
p
< 0.05). The highest prevalence of infection using microscopy and PCR both occurred in school-age children aged 5–8 years (3.6% and 47.8% respectively), while the lowest prevalence for both methods occurred in participants aged 17 – 20 years (0% for both methods) (
p
< 0.05). This study has shown PCR to be effective in detecting schistosomiasis infection and also re-affirms the endemicity of urinary schistosomiasis in the three Senatorial Districts of CRS.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>35299919</pmid><doi>10.1007/s12639-021-01446-2</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3774-5121</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; PubMed Central |
| subjects | Children Health Promotion and Disease Prevention Infections Infectious Diseases Medicine Medicine & Public Health Microscopy Original Original Article Polymerase chain reaction Schistosomiasis |
| title | Detecting Schistosoma haematobium infection by microscopy and polymerase chain reaction (PCR) in school children in three senatorial districts of Cross River State, Nigeria |
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