The Microbiome of Meibomian Gland Secretions from Patients with Internal Hordeolum Treated with Hypochlorous Acid Eyelid Wipes
Objective. The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hord...
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| Veröffentlicht in: | Disease markers 2022, Vol.2022, p.7550090-8 |
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| creator | Yang, Shu Wu, Bing-Cheng Cheng, Zhe Li, Lan Zhang, Yuan-Ping Zhao, Hui Zeng, Han-Mei Qi, Dong-Fang Ma, Zi-Yao Li, Jian-Guo Han, Rui Qu, Fang-Zhou Luo, Yan Liu, Yi Chen, Xiao-Lei Dai, Hong-Mei |
| description | Objective. The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hordeolum. Methods. This was a prospective, matched-pair study. A total of eight patients with internal hordeolum who attended the ophthalmology clinic of our hospital from April to August 2020 were included. Meibomian gland secretions were collected from subjects before treatment (Group A) and from patients cured after eyelid cleaning with hypochlorous acid eyelid wipes for 7 days (Group B). Samples were submitted to 16S rRNA high-throughput sequencing, and the resulting data were analyzed to compare the differences in the structure and composition of meibomian gland secretion microbial flora before and after treatment of internal hordeolum. Results. A total of 2127 operational taxonomic units were obtained from the two groups of samples, and there was no significant difference in alpha diversity before and after eyelid cleaning. At the phylum level, there was no significant difference between the two groups. The predominant phyla in Group A included the following: Firmicutes (32.78%±20.16%), Proteobacteria (26.73%±7.49%), Acidobacteria (10.58%±11.45%), Bacteroidetes (9.05%±6.63%), Actinobacteria (8.48% ±1.77%), and Chloroflexi (3.15%±3.12%), while those in Group B were the following: Proteobacteria (31.86%±9.69%), Firmicutes (29.07%±24.20%), Acidobacteria (11.33%±7.53%), Actinobacteria (7.10%±1.98%), Bacteroidetes (5.39%±5.17%), and Chloroflexi (3.89%±3.67%). Starting from the class level, significant differences in microbial communities were detected before and after eyelid cleaning (P |
| doi_str_mv | 10.1155/2022/7550090 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8894068</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2636150794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c448t-1136918d3344de073d2fbfb22a39bdaaf3e6b5dcd72d8571ba9f02df4008d6283</originalsourceid><addsrcrecordid>eNp9kc9rFDEcxYModlu9eZaAF0HH5uckcxFKqd1Ci4IrHkNm8h0nZWayTWYse-nfbpZZi3rw9A7fD4_vew-hV5R8oFTKU0YYO1VSElKRJ2hFtZKFLjl5ilaEKV0QJsgROk7plhDKKlE9R0dcMkm5KlfoYdMBvvFNDLUPA-DQ4hvwdRi8HfFlb0eHv0ITYfJhTLiNYcBf7ORhnBK-91OHr8YJ4mh7vA7RQejnAW8i2Anccl_vtqHp-hDDnPBZ4x2-2EGf5bvfQnqBnrW2T_DyoCfo26eLzfm6uP58eXV-dl00QuipoJSXFdWOcyEcEMUda-u2ZszyqnbWthzKWrrGKea0VLS2VUuYawUh2pVM8xP0cfHdzvUArsn_R9ubbfSDjTsTrDd_X0bfmR_hp9G6EqTcG7w9GMRwN0OazOBTA31uCHIyw0peaqVLKjP65h_0Nsz7ihaKSqIqkan3C5WrTylC-_gMJWY_rNkPaw7DZvz1nwEe4d9LZuDdAnR-dPbe_9_uF8_nrL0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2636150794</pqid></control><display><type>article</type><title>The Microbiome of Meibomian Gland Secretions from Patients with Internal Hordeolum Treated with Hypochlorous Acid Eyelid Wipes</title><source>MEDLINE</source><source>Wiley Online Library Open Access</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Yang, Shu ; Wu, Bing-Cheng ; Cheng, Zhe ; Li, Lan ; Zhang, Yuan-Ping ; Zhao, Hui ; Zeng, Han-Mei ; Qi, Dong-Fang ; Ma, Zi-Yao ; Li, Jian-Guo ; Han, Rui ; Qu, Fang-Zhou ; Luo, Yan ; Liu, Yi ; Chen, Xiao-Lei ; Dai, Hong-Mei</creator><contributor>Shi, Wen-Qing ; Wen-Qing Shi</contributor><creatorcontrib>Yang, Shu ; Wu, Bing-Cheng ; Cheng, Zhe ; Li, Lan ; Zhang, Yuan-Ping ; Zhao, Hui ; Zeng, Han-Mei ; Qi, Dong-Fang ; Ma, Zi-Yao ; Li, Jian-Guo ; Han, Rui ; Qu, Fang-Zhou ; Luo, Yan ; Liu, Yi ; Chen, Xiao-Lei ; Dai, Hong-Mei ; Shi, Wen-Qing ; Wen-Qing Shi</creatorcontrib><description>Objective. The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hordeolum. Methods. This was a prospective, matched-pair study. A total of eight patients with internal hordeolum who attended the ophthalmology clinic of our hospital from April to August 2020 were included. Meibomian gland secretions were collected from subjects before treatment (Group A) and from patients cured after eyelid cleaning with hypochlorous acid eyelid wipes for 7 days (Group B). Samples were submitted to 16S rRNA high-throughput sequencing, and the resulting data were analyzed to compare the differences in the structure and composition of meibomian gland secretion microbial flora before and after treatment of internal hordeolum. Results. A total of 2127 operational taxonomic units were obtained from the two groups of samples, and there was no significant difference in alpha diversity before and after eyelid cleaning. At the phylum level, there was no significant difference between the two groups. The predominant phyla in Group A included the following: Firmicutes (32.78%±20.16%), Proteobacteria (26.73%±7.49%), Acidobacteria (10.58%±11.45%), Bacteroidetes (9.05%±6.63%), Actinobacteria (8.48% ±1.77%), and Chloroflexi (3.15%±3.12%), while those in Group B were the following: Proteobacteria (31.86%±9.69%), Firmicutes (29.07%±24.20%), Acidobacteria (11.33%±7.53%), Actinobacteria (7.10%±1.98%), Bacteroidetes (5.39%±5.17%), and Chloroflexi (3.89%±3.67%). Starting from the class level, significant differences in microbial communities were detected before and after eyelid cleaning (P<0.05). Linear discriminant analysis effect size analysis showed the core flora in Group A microbiome comprising Actinobacteria, Staphylococcus, Staphylococcaceae, Staphylococcus aureus, Ruminococcacea UCg-014, Ruminococcacea-UCG-014, Halomonadaceae, Neisseria, Methylobacterium, Frankiales, and Neisseria sicca, while those in Group B microbial were Streptococcus sp., Blautia, Bifidobacterium pseudocatenulatum, Subdoligranulum, Subdoligranulum variabile, Faecalibacterium, and Faecalibacterium prausnitzii. Conclusion. Eyelid cleaning with hypochlorous acid eyelid wipes does not change the biodiversity in the meibomian gland secretions of patients with internal hordeolum. Hypochlorous acid eyelid wipes may affect the internal hordeolum through broad-spectrum antibacterial action to effectively reduce the relative abundance of symbiotic pathogens, such as Staphylococcus, Neisseria, Actinomycetes, and Ruminococcus and increase that of Faecalibacterium prausnitzii and other symbiotic probiotics with anti-inflammatory effects.</description><identifier>ISSN: 0278-0240</identifier><identifier>EISSN: 1875-8630</identifier><identifier>DOI: 10.1155/2022/7550090</identifier><identifier>PMID: 35251376</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Acidobacteria ; Acids ; Actinobacteria ; Actinomycetes ; Adult ; Antibiotics ; Antiinfectives and antibacterials ; Bacteria - genetics ; Bacteroidetes ; Biodiversity ; Chloroflexi ; Cleaning ; Discriminant analysis ; Eyelid ; Faecalibacterium prausnitzii ; Female ; Females ; Firmicutes ; Flora ; Glands ; Hordeolum - drug therapy ; Humans ; Hypochlorous acid ; Hypochlorous Acid - therapeutic use ; Inflammation ; Male ; Males ; Meibomian Glands - microbiology ; Microbial activity ; Microbiomes ; Microbiota ; Microorganisms ; Neisseria ; Next-generation sequencing ; Ophthalmology ; Oxidants - therapeutic use ; Pathogenesis ; Pathogens ; Patients ; Probiotics ; Prospective Studies ; Proteobacteria ; Relative abundance ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Secretions ; Software ; Staphylococcus</subject><ispartof>Disease markers, 2022, Vol.2022, p.7550090-8</ispartof><rights>Copyright © 2022 Shu Yang et al.</rights><rights>Copyright © 2022 Shu Yang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Shu Yang et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-1136918d3344de073d2fbfb22a39bdaaf3e6b5dcd72d8571ba9f02df4008d6283</citedby><cites>FETCH-LOGICAL-c448t-1136918d3344de073d2fbfb22a39bdaaf3e6b5dcd72d8571ba9f02df4008d6283</cites><orcidid>0000-0001-9865-3728 ; 0000-0003-0739-0147 ; 0000-0002-0206-2606 ; 0000-0002-1641-6567 ; 0000-0003-4009-3339</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894068/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894068/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35251376$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Shi, Wen-Qing</contributor><contributor>Wen-Qing Shi</contributor><creatorcontrib>Yang, Shu</creatorcontrib><creatorcontrib>Wu, Bing-Cheng</creatorcontrib><creatorcontrib>Cheng, Zhe</creatorcontrib><creatorcontrib>Li, Lan</creatorcontrib><creatorcontrib>Zhang, Yuan-Ping</creatorcontrib><creatorcontrib>Zhao, Hui</creatorcontrib><creatorcontrib>Zeng, Han-Mei</creatorcontrib><creatorcontrib>Qi, Dong-Fang</creatorcontrib><creatorcontrib>Ma, Zi-Yao</creatorcontrib><creatorcontrib>Li, Jian-Guo</creatorcontrib><creatorcontrib>Han, Rui</creatorcontrib><creatorcontrib>Qu, Fang-Zhou</creatorcontrib><creatorcontrib>Luo, Yan</creatorcontrib><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Chen, Xiao-Lei</creatorcontrib><creatorcontrib>Dai, Hong-Mei</creatorcontrib><title>The Microbiome of Meibomian Gland Secretions from Patients with Internal Hordeolum Treated with Hypochlorous Acid Eyelid Wipes</title><title>Disease markers</title><addtitle>Dis Markers</addtitle><description>Objective. The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hordeolum. Methods. This was a prospective, matched-pair study. A total of eight patients with internal hordeolum who attended the ophthalmology clinic of our hospital from April to August 2020 were included. Meibomian gland secretions were collected from subjects before treatment (Group A) and from patients cured after eyelid cleaning with hypochlorous acid eyelid wipes for 7 days (Group B). Samples were submitted to 16S rRNA high-throughput sequencing, and the resulting data were analyzed to compare the differences in the structure and composition of meibomian gland secretion microbial flora before and after treatment of internal hordeolum. Results. A total of 2127 operational taxonomic units were obtained from the two groups of samples, and there was no significant difference in alpha diversity before and after eyelid cleaning. At the phylum level, there was no significant difference between the two groups. The predominant phyla in Group A included the following: Firmicutes (32.78%±20.16%), Proteobacteria (26.73%±7.49%), Acidobacteria (10.58%±11.45%), Bacteroidetes (9.05%±6.63%), Actinobacteria (8.48% ±1.77%), and Chloroflexi (3.15%±3.12%), while those in Group B were the following: Proteobacteria (31.86%±9.69%), Firmicutes (29.07%±24.20%), Acidobacteria (11.33%±7.53%), Actinobacteria (7.10%±1.98%), Bacteroidetes (5.39%±5.17%), and Chloroflexi (3.89%±3.67%). Starting from the class level, significant differences in microbial communities were detected before and after eyelid cleaning (P<0.05). Linear discriminant analysis effect size analysis showed the core flora in Group A microbiome comprising Actinobacteria, Staphylococcus, Staphylococcaceae, Staphylococcus aureus, Ruminococcacea UCg-014, Ruminococcacea-UCG-014, Halomonadaceae, Neisseria, Methylobacterium, Frankiales, and Neisseria sicca, while those in Group B microbial were Streptococcus sp., Blautia, Bifidobacterium pseudocatenulatum, Subdoligranulum, Subdoligranulum variabile, Faecalibacterium, and Faecalibacterium prausnitzii. Conclusion. Eyelid cleaning with hypochlorous acid eyelid wipes does not change the biodiversity in the meibomian gland secretions of patients with internal hordeolum. Hypochlorous acid eyelid wipes may affect the internal hordeolum through broad-spectrum antibacterial action to effectively reduce the relative abundance of symbiotic pathogens, such as Staphylococcus, Neisseria, Actinomycetes, and Ruminococcus and increase that of Faecalibacterium prausnitzii and other symbiotic probiotics with anti-inflammatory effects.</description><subject>Acidobacteria</subject><subject>Acids</subject><subject>Actinobacteria</subject><subject>Actinomycetes</subject><subject>Adult</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacteria - genetics</subject><subject>Bacteroidetes</subject><subject>Biodiversity</subject><subject>Chloroflexi</subject><subject>Cleaning</subject><subject>Discriminant analysis</subject><subject>Eyelid</subject><subject>Faecalibacterium prausnitzii</subject><subject>Female</subject><subject>Females</subject><subject>Firmicutes</subject><subject>Flora</subject><subject>Glands</subject><subject>Hordeolum - drug therapy</subject><subject>Humans</subject><subject>Hypochlorous acid</subject><subject>Hypochlorous Acid - therapeutic use</subject><subject>Inflammation</subject><subject>Male</subject><subject>Males</subject><subject>Meibomian Glands - microbiology</subject><subject>Microbial activity</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Neisseria</subject><subject>Next-generation sequencing</subject><subject>Ophthalmology</subject><subject>Oxidants - therapeutic use</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Probiotics</subject><subject>Prospective Studies</subject><subject>Proteobacteria</subject><subject>Relative abundance</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Secretions</subject><subject>Software</subject><subject>Staphylococcus</subject><issn>0278-0240</issn><issn>1875-8630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><recordid>eNp9kc9rFDEcxYModlu9eZaAF0HH5uckcxFKqd1Ci4IrHkNm8h0nZWayTWYse-nfbpZZi3rw9A7fD4_vew-hV5R8oFTKU0YYO1VSElKRJ2hFtZKFLjl5ilaEKV0QJsgROk7plhDKKlE9R0dcMkm5KlfoYdMBvvFNDLUPA-DQ4hvwdRi8HfFlb0eHv0ITYfJhTLiNYcBf7ORhnBK-91OHr8YJ4mh7vA7RQejnAW8i2Anccl_vtqHp-hDDnPBZ4x2-2EGf5bvfQnqBnrW2T_DyoCfo26eLzfm6uP58eXV-dl00QuipoJSXFdWOcyEcEMUda-u2ZszyqnbWthzKWrrGKea0VLS2VUuYawUh2pVM8xP0cfHdzvUArsn_R9ubbfSDjTsTrDd_X0bfmR_hp9G6EqTcG7w9GMRwN0OazOBTA31uCHIyw0peaqVLKjP65h_0Nsz7ihaKSqIqkan3C5WrTylC-_gMJWY_rNkPaw7DZvz1nwEe4d9LZuDdAnR-dPbe_9_uF8_nrL0</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Yang, Shu</creator><creator>Wu, Bing-Cheng</creator><creator>Cheng, Zhe</creator><creator>Li, Lan</creator><creator>Zhang, Yuan-Ping</creator><creator>Zhao, Hui</creator><creator>Zeng, Han-Mei</creator><creator>Qi, Dong-Fang</creator><creator>Ma, Zi-Yao</creator><creator>Li, Jian-Guo</creator><creator>Han, Rui</creator><creator>Qu, Fang-Zhou</creator><creator>Luo, Yan</creator><creator>Liu, Yi</creator><creator>Chen, Xiao-Lei</creator><creator>Dai, Hong-Mei</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9865-3728</orcidid><orcidid>https://orcid.org/0000-0003-0739-0147</orcidid><orcidid>https://orcid.org/0000-0002-0206-2606</orcidid><orcidid>https://orcid.org/0000-0002-1641-6567</orcidid><orcidid>https://orcid.org/0000-0003-4009-3339</orcidid></search><sort><creationdate>2022</creationdate><title>The Microbiome of Meibomian Gland Secretions from Patients with Internal Hordeolum Treated with Hypochlorous Acid Eyelid Wipes</title><author>Yang, Shu ; Wu, Bing-Cheng ; Cheng, Zhe ; Li, Lan ; Zhang, Yuan-Ping ; Zhao, Hui ; Zeng, Han-Mei ; Qi, Dong-Fang ; Ma, Zi-Yao ; Li, Jian-Guo ; Han, Rui ; Qu, Fang-Zhou ; Luo, Yan ; Liu, Yi ; Chen, Xiao-Lei ; Dai, Hong-Mei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-1136918d3344de073d2fbfb22a39bdaaf3e6b5dcd72d8571ba9f02df4008d6283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acidobacteria</topic><topic>Acids</topic><topic>Actinobacteria</topic><topic>Actinomycetes</topic><topic>Adult</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Bacteria - genetics</topic><topic>Bacteroidetes</topic><topic>Biodiversity</topic><topic>Chloroflexi</topic><topic>Cleaning</topic><topic>Discriminant analysis</topic><topic>Eyelid</topic><topic>Faecalibacterium prausnitzii</topic><topic>Female</topic><topic>Females</topic><topic>Firmicutes</topic><topic>Flora</topic><topic>Glands</topic><topic>Hordeolum - drug therapy</topic><topic>Humans</topic><topic>Hypochlorous acid</topic><topic>Hypochlorous Acid - therapeutic use</topic><topic>Inflammation</topic><topic>Male</topic><topic>Males</topic><topic>Meibomian Glands - microbiology</topic><topic>Microbial activity</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Neisseria</topic><topic>Next-generation sequencing</topic><topic>Ophthalmology</topic><topic>Oxidants - therapeutic use</topic><topic>Pathogenesis</topic><topic>Pathogens</topic><topic>Patients</topic><topic>Probiotics</topic><topic>Prospective Studies</topic><topic>Proteobacteria</topic><topic>Relative abundance</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Secretions</topic><topic>Software</topic><topic>Staphylococcus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Shu</creatorcontrib><creatorcontrib>Wu, Bing-Cheng</creatorcontrib><creatorcontrib>Cheng, Zhe</creatorcontrib><creatorcontrib>Li, Lan</creatorcontrib><creatorcontrib>Zhang, Yuan-Ping</creatorcontrib><creatorcontrib>Zhao, Hui</creatorcontrib><creatorcontrib>Zeng, Han-Mei</creatorcontrib><creatorcontrib>Qi, Dong-Fang</creatorcontrib><creatorcontrib>Ma, Zi-Yao</creatorcontrib><creatorcontrib>Li, Jian-Guo</creatorcontrib><creatorcontrib>Han, Rui</creatorcontrib><creatorcontrib>Qu, Fang-Zhou</creatorcontrib><creatorcontrib>Luo, Yan</creatorcontrib><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Chen, Xiao-Lei</creatorcontrib><creatorcontrib>Dai, Hong-Mei</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Shu</au><au>Wu, Bing-Cheng</au><au>Cheng, Zhe</au><au>Li, Lan</au><au>Zhang, Yuan-Ping</au><au>Zhao, Hui</au><au>Zeng, Han-Mei</au><au>Qi, Dong-Fang</au><au>Ma, Zi-Yao</au><au>Li, Jian-Guo</au><au>Han, Rui</au><au>Qu, Fang-Zhou</au><au>Luo, Yan</au><au>Liu, Yi</au><au>Chen, Xiao-Lei</au><au>Dai, Hong-Mei</au><au>Shi, Wen-Qing</au><au>Wen-Qing Shi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Microbiome of Meibomian Gland Secretions from Patients with Internal Hordeolum Treated with Hypochlorous Acid Eyelid Wipes</atitle><jtitle>Disease markers</jtitle><addtitle>Dis Markers</addtitle><date>2022</date><risdate>2022</risdate><volume>2022</volume><spage>7550090</spage><epage>8</epage><pages>7550090-8</pages><issn>0278-0240</issn><eissn>1875-8630</eissn><abstract>Objective. The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hordeolum. Methods. This was a prospective, matched-pair study. A total of eight patients with internal hordeolum who attended the ophthalmology clinic of our hospital from April to August 2020 were included. Meibomian gland secretions were collected from subjects before treatment (Group A) and from patients cured after eyelid cleaning with hypochlorous acid eyelid wipes for 7 days (Group B). Samples were submitted to 16S rRNA high-throughput sequencing, and the resulting data were analyzed to compare the differences in the structure and composition of meibomian gland secretion microbial flora before and after treatment of internal hordeolum. Results. A total of 2127 operational taxonomic units were obtained from the two groups of samples, and there was no significant difference in alpha diversity before and after eyelid cleaning. At the phylum level, there was no significant difference between the two groups. The predominant phyla in Group A included the following: Firmicutes (32.78%±20.16%), Proteobacteria (26.73%±7.49%), Acidobacteria (10.58%±11.45%), Bacteroidetes (9.05%±6.63%), Actinobacteria (8.48% ±1.77%), and Chloroflexi (3.15%±3.12%), while those in Group B were the following: Proteobacteria (31.86%±9.69%), Firmicutes (29.07%±24.20%), Acidobacteria (11.33%±7.53%), Actinobacteria (7.10%±1.98%), Bacteroidetes (5.39%±5.17%), and Chloroflexi (3.89%±3.67%). Starting from the class level, significant differences in microbial communities were detected before and after eyelid cleaning (P<0.05). Linear discriminant analysis effect size analysis showed the core flora in Group A microbiome comprising Actinobacteria, Staphylococcus, Staphylococcaceae, Staphylococcus aureus, Ruminococcacea UCg-014, Ruminococcacea-UCG-014, Halomonadaceae, Neisseria, Methylobacterium, Frankiales, and Neisseria sicca, while those in Group B microbial were Streptococcus sp., Blautia, Bifidobacterium pseudocatenulatum, Subdoligranulum, Subdoligranulum variabile, Faecalibacterium, and Faecalibacterium prausnitzii. Conclusion. Eyelid cleaning with hypochlorous acid eyelid wipes does not change the biodiversity in the meibomian gland secretions of patients with internal hordeolum. Hypochlorous acid eyelid wipes may affect the internal hordeolum through broad-spectrum antibacterial action to effectively reduce the relative abundance of symbiotic pathogens, such as Staphylococcus, Neisseria, Actinomycetes, and Ruminococcus and increase that of Faecalibacterium prausnitzii and other symbiotic probiotics with anti-inflammatory effects.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>35251376</pmid><doi>10.1155/2022/7550090</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9865-3728</orcidid><orcidid>https://orcid.org/0000-0003-0739-0147</orcidid><orcidid>https://orcid.org/0000-0002-0206-2606</orcidid><orcidid>https://orcid.org/0000-0002-1641-6567</orcidid><orcidid>https://orcid.org/0000-0003-4009-3339</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0278-0240 |
| ispartof | Disease markers, 2022, Vol.2022, p.7550090-8 |
| issn | 0278-0240 1875-8630 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8894068 |
| source | MEDLINE; Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Acidobacteria Acids Actinobacteria Actinomycetes Adult Antibiotics Antiinfectives and antibacterials Bacteria - genetics Bacteroidetes Biodiversity Chloroflexi Cleaning Discriminant analysis Eyelid Faecalibacterium prausnitzii Female Females Firmicutes Flora Glands Hordeolum - drug therapy Humans Hypochlorous acid Hypochlorous Acid - therapeutic use Inflammation Male Males Meibomian Glands - microbiology Microbial activity Microbiomes Microbiota Microorganisms Neisseria Next-generation sequencing Ophthalmology Oxidants - therapeutic use Pathogenesis Pathogens Patients Probiotics Prospective Studies Proteobacteria Relative abundance RNA, Ribosomal, 16S - genetics rRNA 16S Secretions Software Staphylococcus |
| title | The Microbiome of Meibomian Gland Secretions from Patients with Internal Hordeolum Treated with Hypochlorous Acid Eyelid Wipes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A37%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Microbiome%20of%20Meibomian%20Gland%20Secretions%20from%20Patients%20with%20Internal%20Hordeolum%20Treated%20with%20Hypochlorous%20Acid%20Eyelid%20Wipes&rft.jtitle=Disease%20markers&rft.au=Yang,%20Shu&rft.date=2022&rft.volume=2022&rft.spage=7550090&rft.epage=8&rft.pages=7550090-8&rft.issn=0278-0240&rft.eissn=1875-8630&rft_id=info:doi/10.1155/2022/7550090&rft_dat=%3Cproquest_pubme%3E2636150794%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2636150794&rft_id=info:pmid/35251376&rfr_iscdi=true |