Study of the Role of Antimicrobial Glucosinolate-Derived Isothiocyanates in Resistance of Arabidopsis to Microbial Pathogens
Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundanc...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2001-04, Vol.125 (4), p.1688-1699 |
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| creator | Koenraad F. M.-J. Tierens Bart P. H. J. Thomma Brouwer, Margreet Schmidt, Jürgen Katherine Kistner Andrea Porzel Mauch-Mani, Brigitte Bruno P. A. Cammue Broekaert, Willem F. |
| description | Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from untreated plants versus leaves from plants challenge inoculated with the fungus Alternaria brassicicola. One of both major antimicrobial fractions was purified to homogeneity and identified by 1H nuclear magnetic resonance, gas chromatography/electron impact mass spectrometry, and gas chromatography/chemical ionization mass spectrometry as 4-methylsulphinylbutyl isothiocyanate (ITC). This compound has previously been described as a product of myrosinase-mediated breakdown of glucoraphanin, the predominant glucosinolate in Arabidopsis leaves. 4-Methylsulphinylbutyl ITC was found to be inhibitory to a wide range of fungi and bacteria, producing 50% growth inhibition in vitro at concentrations of 28 μM for the most sensitive organism tested (Pseudomonas syringae). A previously identified glucosinolate biosynthesis mutant, gsm1-1, was found to be largely deficient in either of the two major antimicrobial compounds, including 4-methylsulphinylbutyl ITC. The resistance of gsm1-1 was compared with that of wild-type plants after challenge with the fungi A. brassicicola, Plectosphaerella cucumerina, Botrytis cinerea, Fusarium oxysporum, or Peronospora parasitica, or the bacteria Erwinia carotovora or P. syringae. Of the tested pathogens, only F. oxysporum was found to be significantly more aggressive on gsm1-1 than on wild-type plants. Taken together, our data suggest that glucosinolate-derived antimicrobial ITCs can play a role in the protection of Arabidopsis against particular pathogens. |
| doi_str_mv | 10.1104/pp.125.4.1688 |
| format | Article |
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M.-J. Tierens ; Bart P. H. J. Thomma ; Brouwer, Margreet ; Schmidt, Jürgen ; Katherine Kistner ; Andrea Porzel ; Mauch-Mani, Brigitte ; Bruno P. A. Cammue ; Broekaert, Willem F.</creator><creatorcontrib>Koenraad F. M.-J. Tierens ; Bart P. H. J. Thomma ; Brouwer, Margreet ; Schmidt, Jürgen ; Katherine Kistner ; Andrea Porzel ; Mauch-Mani, Brigitte ; Bruno P. A. Cammue ; Broekaert, Willem F.</creatorcontrib><description>Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from untreated plants versus leaves from plants challenge inoculated with the fungus Alternaria brassicicola. One of both major antimicrobial fractions was purified to homogeneity and identified by 1H nuclear magnetic resonance, gas chromatography/electron impact mass spectrometry, and gas chromatography/chemical ionization mass spectrometry as 4-methylsulphinylbutyl isothiocyanate (ITC). This compound has previously been described as a product of myrosinase-mediated breakdown of glucoraphanin, the predominant glucosinolate in Arabidopsis leaves. 4-Methylsulphinylbutyl ITC was found to be inhibitory to a wide range of fungi and bacteria, producing 50% growth inhibition in vitro at concentrations of 28 μM for the most sensitive organism tested (Pseudomonas syringae). A previously identified glucosinolate biosynthesis mutant, gsm1-1, was found to be largely deficient in either of the two major antimicrobial compounds, including 4-methylsulphinylbutyl ITC. The resistance of gsm1-1 was compared with that of wild-type plants after challenge with the fungi A. brassicicola, Plectosphaerella cucumerina, Botrytis cinerea, Fusarium oxysporum, or Peronospora parasitica, or the bacteria Erwinia carotovora or P. syringae. Of the tested pathogens, only F. oxysporum was found to be significantly more aggressive on gsm1-1 than on wild-type plants. Taken together, our data suggest that glucosinolate-derived antimicrobial ITCs can play a role in the protection of Arabidopsis against particular pathogens.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.125.4.1688</identifier><identifier>PMID: 11299350</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Antifungals ; Antimicrobials ; Arabidopsis - microbiology ; Arabidopsis - physiology ; Autoecology ; Biological and medical sciences ; Biosynthesis ; Erwinia - drug effects ; Erwinia - pathogenicity ; Escherichia coli - drug effects ; Fundamental and applied biological sciences. Psychology ; Fungi ; Fungi - drug effects ; Fungi - pathogenicity ; Gas chromatography ; Generalities. Disease free stocks ; Glucosinolates ; Glucosinolates - isolation & purification ; Glucosinolates - pharmacology ; Immunity, Innate ; Inoculation ; Ionization ; Isothiocyanates - isolation & purification ; Isothiocyanates - pharmacology ; Leaves ; Mass spectrometry ; Microbial Sensitivity Tests ; NMR ; Nuclear magnetic resonance ; Oilseeds ; Parasitism ; Pathogens ; Phytopathology. Animal pests. Plant and forest protection ; Plant Extracts - chemistry ; Plant Leaves - chemistry ; Plant Leaves - physiology ; Plants ; Plants and fungi ; Plants Interacting with Other Organisms ; Pseudomonas - drug effects ; Pseudomonas - pathogenicity</subject><ispartof>Plant physiology (Bethesda), 2001-04, Vol.125 (4), p.1688-1699</ispartof><rights>Copyright 2001 American Society of Plant Physiologists</rights><rights>2001 INIST-CNRS</rights><rights>Copyright American Society of Plant Physiologists Apr 2001</rights><rights>Copyright © 2001, American Society of Plant Physiologists 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-2421c92cfc649d06c22389f33eca46444edd26f47e9dbf5ca665e38eab9548753</citedby><cites>FETCH-LOGICAL-c560t-2421c92cfc649d06c22389f33eca46444edd26f47e9dbf5ca665e38eab9548753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4279801$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4279801$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=962454$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11299350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koenraad F. M.-J. Tierens</creatorcontrib><creatorcontrib>Bart P. H. J. Thomma</creatorcontrib><creatorcontrib>Brouwer, Margreet</creatorcontrib><creatorcontrib>Schmidt, Jürgen</creatorcontrib><creatorcontrib>Katherine Kistner</creatorcontrib><creatorcontrib>Andrea Porzel</creatorcontrib><creatorcontrib>Mauch-Mani, Brigitte</creatorcontrib><creatorcontrib>Bruno P. A. Cammue</creatorcontrib><creatorcontrib>Broekaert, Willem F.</creatorcontrib><title>Study of the Role of Antimicrobial Glucosinolate-Derived Isothiocyanates in Resistance of Arabidopsis to Microbial Pathogens</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from untreated plants versus leaves from plants challenge inoculated with the fungus Alternaria brassicicola. One of both major antimicrobial fractions was purified to homogeneity and identified by 1H nuclear magnetic resonance, gas chromatography/electron impact mass spectrometry, and gas chromatography/chemical ionization mass spectrometry as 4-methylsulphinylbutyl isothiocyanate (ITC). This compound has previously been described as a product of myrosinase-mediated breakdown of glucoraphanin, the predominant glucosinolate in Arabidopsis leaves. 4-Methylsulphinylbutyl ITC was found to be inhibitory to a wide range of fungi and bacteria, producing 50% growth inhibition in vitro at concentrations of 28 μM for the most sensitive organism tested (Pseudomonas syringae). A previously identified glucosinolate biosynthesis mutant, gsm1-1, was found to be largely deficient in either of the two major antimicrobial compounds, including 4-methylsulphinylbutyl ITC. The resistance of gsm1-1 was compared with that of wild-type plants after challenge with the fungi A. brassicicola, Plectosphaerella cucumerina, Botrytis cinerea, Fusarium oxysporum, or Peronospora parasitica, or the bacteria Erwinia carotovora or P. syringae. Of the tested pathogens, only F. oxysporum was found to be significantly more aggressive on gsm1-1 than on wild-type plants. Taken together, our data suggest that glucosinolate-derived antimicrobial ITCs can play a role in the protection of Arabidopsis against particular pathogens.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Antifungals</subject><subject>Antimicrobials</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis - physiology</subject><subject>Autoecology</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Erwinia - drug effects</subject><subject>Erwinia - pathogenicity</subject><subject>Escherichia coli - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Fungi - drug effects</subject><subject>Fungi - pathogenicity</subject><subject>Gas chromatography</subject><subject>Generalities. Disease free stocks</subject><subject>Glucosinolates</subject><subject>Glucosinolates - isolation & purification</subject><subject>Glucosinolates - pharmacology</subject><subject>Immunity, Innate</subject><subject>Inoculation</subject><subject>Ionization</subject><subject>Isothiocyanates - isolation & purification</subject><subject>Isothiocyanates - pharmacology</subject><subject>Leaves</subject><subject>Mass spectrometry</subject><subject>Microbial Sensitivity Tests</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Oilseeds</subject><subject>Parasitism</subject><subject>Pathogens</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - physiology</subject><subject>Plants</subject><subject>Plants and fungi</subject><subject>Plants Interacting with Other Organisms</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - pathogenicity</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdktFrFDEQxoMo9qw--iayKPi2Z5JNsgn4Uqq2hYpS9Tlks7O9HHvJmmQLB_7x5rjjavuUYeY3HzPzBaHXBC8JwezjNC0J5Uu2JELKJ2hBeENrypl8ihYYlxhLqU7Qi5TWGGPSEPYcnRBClWo4XqC_P_Pcb6swVHkF1U0YYRef-ew2zsbQOTNWF-NsQ3I-jCZD_Rmiu4O-ukohr1ywW-NLOlXOVzeQXMrG271INJ3rw1RyVQ7Vt6PeD5NX4RZ8eomeDWZM8OrwnqLfX7_8Or-sr79fXJ2fXdeWC5xryiixitrBCqZ6LCyljVRD04A1TDDGoO-pGFgLqu8Gbo0QHBoJplPlDi1vTtGnve40dxvoLfgczain6DYmbnUwTj-seLfSt-FOSympKO0fDu0x_JkhZb1xycI4Gg9hTrptMaeY7MB3j8B1mKMvq2lKpBAKy7ZA9R4q50gpwnCcg2C9s1RPky6WaqZ3lhb-7f_D39MHDwvw_gCYZM04xGKAS0dOCco4K9SbPbVOOcRjldFWyfIt_gH6DrVQ</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Koenraad F. 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M.-J. Tierens ; Bart P. H. J. Thomma ; Brouwer, Margreet ; Schmidt, Jürgen ; Katherine Kistner ; Andrea Porzel ; Mauch-Mani, Brigitte ; Bruno P. A. Cammue ; Broekaert, Willem F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-2421c92cfc649d06c22389f33eca46444edd26f47e9dbf5ca665e38eab9548753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Antifungals</topic><topic>Antimicrobials</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis - physiology</topic><topic>Autoecology</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Erwinia - drug effects</topic><topic>Erwinia - pathogenicity</topic><topic>Escherichia coli - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Fungi - drug effects</topic><topic>Fungi - pathogenicity</topic><topic>Gas chromatography</topic><topic>Generalities. Disease free stocks</topic><topic>Glucosinolates</topic><topic>Glucosinolates - isolation & purification</topic><topic>Glucosinolates - pharmacology</topic><topic>Immunity, Innate</topic><topic>Inoculation</topic><topic>Ionization</topic><topic>Isothiocyanates - isolation & purification</topic><topic>Isothiocyanates - pharmacology</topic><topic>Leaves</topic><topic>Mass spectrometry</topic><topic>Microbial Sensitivity Tests</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Oilseeds</topic><topic>Parasitism</topic><topic>Pathogens</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - physiology</topic><topic>Plants</topic><topic>Plants and fungi</topic><topic>Plants Interacting with Other Organisms</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koenraad F. M.-J. Tierens</creatorcontrib><creatorcontrib>Bart P. H. J. Thomma</creatorcontrib><creatorcontrib>Brouwer, Margreet</creatorcontrib><creatorcontrib>Schmidt, Jürgen</creatorcontrib><creatorcontrib>Katherine Kistner</creatorcontrib><creatorcontrib>Andrea Porzel</creatorcontrib><creatorcontrib>Mauch-Mani, Brigitte</creatorcontrib><creatorcontrib>Bruno P. A. 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M.-J. Tierens</au><au>Bart P. H. J. Thomma</au><au>Brouwer, Margreet</au><au>Schmidt, Jürgen</au><au>Katherine Kistner</au><au>Andrea Porzel</au><au>Mauch-Mani, Brigitte</au><au>Bruno P. A. Cammue</au><au>Broekaert, Willem F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of the Role of Antimicrobial Glucosinolate-Derived Isothiocyanates in Resistance of Arabidopsis to Microbial Pathogens</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>125</volume><issue>4</issue><spage>1688</spage><epage>1699</epage><pages>1688-1699</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from untreated plants versus leaves from plants challenge inoculated with the fungus Alternaria brassicicola. One of both major antimicrobial fractions was purified to homogeneity and identified by 1H nuclear magnetic resonance, gas chromatography/electron impact mass spectrometry, and gas chromatography/chemical ionization mass spectrometry as 4-methylsulphinylbutyl isothiocyanate (ITC). This compound has previously been described as a product of myrosinase-mediated breakdown of glucoraphanin, the predominant glucosinolate in Arabidopsis leaves. 4-Methylsulphinylbutyl ITC was found to be inhibitory to a wide range of fungi and bacteria, producing 50% growth inhibition in vitro at concentrations of 28 μM for the most sensitive organism tested (Pseudomonas syringae). A previously identified glucosinolate biosynthesis mutant, gsm1-1, was found to be largely deficient in either of the two major antimicrobial compounds, including 4-methylsulphinylbutyl ITC. The resistance of gsm1-1 was compared with that of wild-type plants after challenge with the fungi A. brassicicola, Plectosphaerella cucumerina, Botrytis cinerea, Fusarium oxysporum, or Peronospora parasitica, or the bacteria Erwinia carotovora or P. syringae. Of the tested pathogens, only F. oxysporum was found to be significantly more aggressive on gsm1-1 than on wild-type plants. Taken together, our data suggest that glucosinolate-derived antimicrobial ITCs can play a role in the protection of Arabidopsis against particular pathogens.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>11299350</pmid><doi>10.1104/pp.125.4.1688</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 2001-04, Vol.125 (4), p.1688-1699 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_88826 |
| source | Jstor Complete Legacy; MEDLINE; EZB Electronic Journals Library; Oxford Journals |
| subjects | Animal and plant ecology Animal, plant and microbial ecology Antifungals Antimicrobials Arabidopsis - microbiology Arabidopsis - physiology Autoecology Biological and medical sciences Biosynthesis Erwinia - drug effects Erwinia - pathogenicity Escherichia coli - drug effects Fundamental and applied biological sciences. Psychology Fungi Fungi - drug effects Fungi - pathogenicity Gas chromatography Generalities. Disease free stocks Glucosinolates Glucosinolates - isolation & purification Glucosinolates - pharmacology Immunity, Innate Inoculation Ionization Isothiocyanates - isolation & purification Isothiocyanates - pharmacology Leaves Mass spectrometry Microbial Sensitivity Tests NMR Nuclear magnetic resonance Oilseeds Parasitism Pathogens Phytopathology. Animal pests. Plant and forest protection Plant Extracts - chemistry Plant Leaves - chemistry Plant Leaves - physiology Plants Plants and fungi Plants Interacting with Other Organisms Pseudomonas - drug effects Pseudomonas - pathogenicity |
| title | Study of the Role of Antimicrobial Glucosinolate-Derived Isothiocyanates in Resistance of Arabidopsis to Microbial Pathogens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T19%3A31%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Study%20of%20the%20Role%20of%20Antimicrobial%20Glucosinolate-Derived%20Isothiocyanates%20in%20Resistance%20of%20Arabidopsis%20to%20Microbial%20Pathogens&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Koenraad%20F.%20M.-J.%20Tierens&rft.date=2001-04-01&rft.volume=125&rft.issue=4&rft.spage=1688&rft.epage=1699&rft.pages=1688-1699&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.125.4.1688&rft_dat=%3Cjstor_pubme%3E4279801%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=218669087&rft_id=info:pmid/11299350&rft_jstor_id=4279801&rfr_iscdi=true |