Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity

Pathogenic superbugs are the root cause of untreatable complex infections with limited or no treatment options. These infections are becoming more common as clinical antibiotics have lost their effectiveness over time. Therefore, the development of novel antibacterial agents is urgently needed to co...

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Veröffentlicht in:International journal of molecular sciences 2022-02, Vol.23 (4), p.2191
Hauptverfasser: Hazam, Prakash Kishore, Cheng, Chin-Cheng, Hsieh, Chu-Yi, Lin, Wen-Chun, Hsu, Po-Hsien, Chen, Te-Li, Lee, Yi-Tzu, Chen, Jyh-Yih
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container_issue 4
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container_title International journal of molecular sciences
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creator Hazam, Prakash Kishore
Cheng, Chin-Cheng
Hsieh, Chu-Yi
Lin, Wen-Chun
Hsu, Po-Hsien
Chen, Te-Li
Lee, Yi-Tzu
Chen, Jyh-Yih
description Pathogenic superbugs are the root cause of untreatable complex infections with limited or no treatment options. These infections are becoming more common as clinical antibiotics have lost their effectiveness over time. Therefore, the development of novel antibacterial agents is urgently needed to counter these microbes. Antimicrobial peptides (AMPs) are a viable treatment option due to their bactericidal potency against multiple microbial classes. AMPs are naturally selected physiological microbicidal agents that are found in all forms of organisms. In the present study, we developed two tilapia piscidin 2 (TP2)-based AMPs for antimicrobial application. Unlike the parent peptide, the redesigned peptides showed significant antimicrobial activity against multidrug-resistant bacterial species. These peptides also showed minimal cytotoxicity. In addition, they were significantly active in the presence of physiological salts, 50% human serum and elevated temperature. The designed peptides also showed synergistic activity when combined with clinical antibiotics. The current approach demonstrates a fruitful strategy for developing potential AMPs for antimicrobial application. Such AMPs have potential for progression to further trials and drug development investigations.
doi_str_mv 10.3390/ijms23042191
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source MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Acinetobacter baumannii
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Anti-Infective Agents - pharmacology
Antibacterial agents
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antimicrobial peptides
Clinical trials
Cytotoxicity
Drug development
Drug resistance
Drug Resistance, Multiple, Bacterial
Heat resistance
High temperature
Humans
Microbial Sensitivity Tests
Microbicides
Microorganisms
Multidrug resistance
Multidrug resistant organisms
Peptides
Physiology
Salts
Surfactants
Tilapia
Toxicity
title Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity
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