Indirect enzyme-linked immunosorbent assay for detection of immunoglobulin G reactive with a recombinant protein expressed from the gene encoding the 116-kilodalton protein of Mycoplasma pneumoniae

Serology remains the method of choice for laboratory diagnosis of Mycoplasma pneumoniae infection. Currently available serological tests employ complex cellular fractions of M. pneumoniae as antigen. To improve the specificity of M. pneumoniae diagnosis, a recombinant protein was assessed as a serod...

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Veröffentlicht in:	Journal of clinical microbiology 1999-04, Vol.37 (4), p.1024-1029
Hauptverfasser:	DUFFY, M. F, WHITHEAR, K. G, NOORMOHAMMADI, A. H, MARKHAM, P. F, CATTON, M, LEYDON, J, BROWNING, G. F
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - blood Antibody Specificity Antigens, Bacterial - chemistry Antigens, Bacterial - genetics Bacterial diseases Bacterial diseases of the respiratory system Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - immunology Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Blotting, Western DNA Primers - genetics Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Genes, Bacterial Human bacterial diseases Humans Immunodominant Epitopes - chemistry Immunodominant Epitopes - genetics Immunoglobulin G - blood Infectious diseases Medical sciences Microbiology Molecular Weight Mycoplasma pneumoniae Mycoplasma pneumoniae - genetics Mycoplasma pneumoniae - immunology Pneumonia, Mycoplasma - diagnosis Pneumonia, Mycoplasma - immunology Polymerase Chain Reaction Rabbits Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Serologic Tests Species Specificity
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