Cordycepin mitigates spermatogenic and redox related expression in H2O2-exposed Leydig cells and regulates testicular oxidative apoptotic signalling in aged rats
Cordycepin (COR), from Cordyceps militaris L., (Cordycipitaceae), is a valuable agent with immense health benefits. The protective effects of COR in ageing-associated oxidative and apoptosis events in vivo and hydrogen peroxide (H 2 O 2 )-exposed spermatogenesis gene alterations in TM3 Leydig cells...
Gespeichert in:
| Veröffentlicht in: | Pharmaceutical biology 2022-12, Vol.60 (1), p.404-416 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 416 |
|---|---|
| container_issue | 1 |
| container_start_page | 404 |
| container_title | Pharmaceutical biology |
| container_volume | 60 |
| creator | Kopalli, Spandana Rajendra Cha, Kyu-Min Cho, Jae Youl Kim, Si-Kwan Koppula, Sushruta |
| description | Cordycepin (COR), from Cordyceps militaris L., (Cordycipitaceae), is a valuable agent with immense health benefits.
The protective effects of COR in ageing-associated oxidative and apoptosis events in vivo and hydrogen peroxide (H
2
O
2
)-exposed spermatogenesis gene alterations in TM3 Leydig cells was investigated.
Male Sprague-Dawley rats were divided into young control (YC), aged control (AC) and COR treated (COR-20) aged groups. COR-20 group received daily doses of COR (20 mg/kg) for 6 months. Cell viability and hormone levels were analysed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and enzyme immunoassay kits with COR treated at 1, 5, and 10 μg/mL. Oxidative enzymes, spermatogenic, and apoptotic expression in testis tissues were evaluated by Western blotting and real-time RT-PCR.
COR treatment (1, 5, and 10 μg/mL) significantly (p |
| doi_str_mv | 10.1080/13880209.2022.2033275 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8863333</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_fa228af70f1942f2868d632c31b4983e</doaj_id><sourcerecordid>2761422960</sourcerecordid><originalsourceid>FETCH-LOGICAL-d394t-207066af6a16ed38fce5249e5c9d24d8c6c1ef5bd9eb5fb0bb1b4445d1813c5c3</originalsourceid><addsrcrecordid>eNpdks1u1DAQxyMEomXhEZAiceGS4o_YcS4ItAJaaaVe4Gw5_gheee1gJ2X3cXhTJt3lUCx7bM_85ydrPFX1FqMbjAT6gKkQiKD-hiBCwFBKOvasusZd2zYMY_4czqBpVtFV9aqUPUKIUcpeVleU4Q4muq7-bFM2J20nH-uDn_2oZlvqMtl8UHMabfS6VtHU2Zp0BBsgbmp7nLItxadYQ94tuScNuFKB0M6ejB9rbUMol8xxCY9UWLPXcMl1OnqjZv9gazWlaU7gr4sfowrBx3GFqhFgWc3ldfXCqVDsm8u-qX58_fJ9e9vs7r_dbT_vGkP7dm4I6hDnynGFuTVUOG0ZaXvLdG9Ia4TmGlvHBtPbgbkBDQMe2rZlBgtMNdN0U92duSapvZyyP6h8kkl5-ehIeZQqwzuDlU4RIpTrkMN9SxwRXBhOiaaA7AW1wPp4Zk3LcLBG2zhnFZ5An0ai_ynH9CCF4HQdm-r9BZDTrwXqJg--rDVV0aalSMJJLzgXjIP03X_SfVoyVBJUHcctIT1HoPp0VvnoEvzt75SDkbM6hZRdVlH7IilGcu0t-a-35Npb8tJb9C-EvcUB</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2761422960</pqid></control><display><type>article</type><title>Cordycepin mitigates spermatogenic and redox related expression in H2O2-exposed Leydig cells and regulates testicular oxidative apoptotic signalling in aged rats</title><source>Taylor & Francis Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Kopalli, Spandana Rajendra ; Cha, Kyu-Min ; Cho, Jae Youl ; Kim, Si-Kwan ; Koppula, Sushruta</creator><creatorcontrib>Kopalli, Spandana Rajendra ; Cha, Kyu-Min ; Cho, Jae Youl ; Kim, Si-Kwan ; Koppula, Sushruta</creatorcontrib><description>Cordycepin (COR), from Cordyceps militaris L., (Cordycipitaceae), is a valuable agent with immense health benefits.
The protective effects of COR in ageing-associated oxidative and apoptosis events in vivo and hydrogen peroxide (H
2
O
2
)-exposed spermatogenesis gene alterations in TM3 Leydig cells was investigated.
Male Sprague-Dawley rats were divided into young control (YC), aged control (AC) and COR treated (COR-20) aged groups. COR-20 group received daily doses of COR (20 mg/kg) for 6 months. Cell viability and hormone levels were analysed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and enzyme immunoassay kits with COR treated at 1, 5, and 10 μg/mL. Oxidative enzymes, spermatogenic, and apoptotic expression in testis tissues were evaluated by Western blotting and real-time RT-PCR.
COR treatment (1, 5, and 10 μg/mL) significantly (p < 0.05 ∼ p < 0.001) inhibited the H
2
O
2
-induced decrease in the percentage of viable cells (from 63.27% to 71.25%, 85.67% and 93.97%, respectively), and reduced the malondialdehyde (MDA) content (from 4.28 to 3.98, 3.14 and 1.78 nM MDA/mg protein, respectively). Further, the decreased antioxidant enzymes (glutathione-S-transferase mu5, glutathione peroxidase 4 and peroxiredoxin 3), spermatogenesis-related factors (nectin-2 and inhibin-α) and testosterone levels in H
2
O
2
-exposed TM3 cells were significantly (p < 0.05 ∼ p < 0.001) ameliorated by COR. In aged rats, COR (20 mg/kg) restored the altered enzymatic and non-enzymatic antioxidative status and attenuated the apoptotic p53 and Bax/Bcl-2 expression significantly (p < 0.05).
COR might be developed as a potential agent against ageing-associated and oxidative stress-induced male infertility.</description><identifier>ISSN: 1388-0209</identifier><identifier>EISSN: 1744-5116</identifier><identifier>DOI: 10.1080/13880209.2022.2033275</identifier><identifier>PMID: 35175170</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Aging ; Apoptosis ; BAX protein ; Bcl-2 protein ; catalase ; Cell viability ; Cordycepin ; Enzyme immunoassay ; Enzymes ; glutathione ; Glutathione peroxidase ; Glutathione transferase ; Hydrogen peroxide ; Infertility ; Inhibin ; Leydig cells ; lipid peroxidation ; Nectin ; Oxidative stress ; p53 Protein ; Peroxiredoxin ; Spermatogenesis ; spermatogenic factors ; Testosterone ; Western blotting</subject><ispartof>Pharmaceutical biology, 2022-12, Vol.60 (1), p.404-416</ispartof><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2022</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2022 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-3379-4797 ; 0000-0001-8141-9927 ; 0000-0003-2361-5748 ; 0000-0003-3237-3015 ; 0000-0002-8597-7763</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863333/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863333/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,27489,27911,27912,53778,53780,59128,59129</link.rule.ids></links><search><creatorcontrib>Kopalli, Spandana Rajendra</creatorcontrib><creatorcontrib>Cha, Kyu-Min</creatorcontrib><creatorcontrib>Cho, Jae Youl</creatorcontrib><creatorcontrib>Kim, Si-Kwan</creatorcontrib><creatorcontrib>Koppula, Sushruta</creatorcontrib><title>Cordycepin mitigates spermatogenic and redox related expression in H2O2-exposed Leydig cells and regulates testicular oxidative apoptotic signalling in aged rats</title><title>Pharmaceutical biology</title><description>Cordycepin (COR), from Cordyceps militaris L., (Cordycipitaceae), is a valuable agent with immense health benefits.
The protective effects of COR in ageing-associated oxidative and apoptosis events in vivo and hydrogen peroxide (H
2
O
2
)-exposed spermatogenesis gene alterations in TM3 Leydig cells was investigated.
Male Sprague-Dawley rats were divided into young control (YC), aged control (AC) and COR treated (COR-20) aged groups. COR-20 group received daily doses of COR (20 mg/kg) for 6 months. Cell viability and hormone levels were analysed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and enzyme immunoassay kits with COR treated at 1, 5, and 10 μg/mL. Oxidative enzymes, spermatogenic, and apoptotic expression in testis tissues were evaluated by Western blotting and real-time RT-PCR.
COR treatment (1, 5, and 10 μg/mL) significantly (p < 0.05 ∼ p < 0.001) inhibited the H
2
O
2
-induced decrease in the percentage of viable cells (from 63.27% to 71.25%, 85.67% and 93.97%, respectively), and reduced the malondialdehyde (MDA) content (from 4.28 to 3.98, 3.14 and 1.78 nM MDA/mg protein, respectively). Further, the decreased antioxidant enzymes (glutathione-S-transferase mu5, glutathione peroxidase 4 and peroxiredoxin 3), spermatogenesis-related factors (nectin-2 and inhibin-α) and testosterone levels in H
2
O
2
-exposed TM3 cells were significantly (p < 0.05 ∼ p < 0.001) ameliorated by COR. In aged rats, COR (20 mg/kg) restored the altered enzymatic and non-enzymatic antioxidative status and attenuated the apoptotic p53 and Bax/Bcl-2 expression significantly (p < 0.05).
COR might be developed as a potential agent against ageing-associated and oxidative stress-induced male infertility.</description><subject>Aging</subject><subject>Apoptosis</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>catalase</subject><subject>Cell viability</subject><subject>Cordycepin</subject><subject>Enzyme immunoassay</subject><subject>Enzymes</subject><subject>glutathione</subject><subject>Glutathione peroxidase</subject><subject>Glutathione transferase</subject><subject>Hydrogen peroxide</subject><subject>Infertility</subject><subject>Inhibin</subject><subject>Leydig cells</subject><subject>lipid peroxidation</subject><subject>Nectin</subject><subject>Oxidative stress</subject><subject>p53 Protein</subject><subject>Peroxiredoxin</subject><subject>Spermatogenesis</subject><subject>spermatogenic factors</subject><subject>Testosterone</subject><subject>Western blotting</subject><issn>1388-0209</issn><issn>1744-5116</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNpdks1u1DAQxyMEomXhEZAiceGS4o_YcS4ItAJaaaVe4Gw5_gheee1gJ2X3cXhTJt3lUCx7bM_85ydrPFX1FqMbjAT6gKkQiKD-hiBCwFBKOvasusZd2zYMY_4czqBpVtFV9aqUPUKIUcpeVleU4Q4muq7-bFM2J20nH-uDn_2oZlvqMtl8UHMabfS6VtHU2Zp0BBsgbmp7nLItxadYQ94tuScNuFKB0M6ejB9rbUMol8xxCY9UWLPXcMl1OnqjZv9gazWlaU7gr4sfowrBx3GFqhFgWc3ldfXCqVDsm8u-qX58_fJ9e9vs7r_dbT_vGkP7dm4I6hDnynGFuTVUOG0ZaXvLdG9Ia4TmGlvHBtPbgbkBDQMe2rZlBgtMNdN0U92duSapvZyyP6h8kkl5-ehIeZQqwzuDlU4RIpTrkMN9SxwRXBhOiaaA7AW1wPp4Zk3LcLBG2zhnFZ5An0ai_ynH9CCF4HQdm-r9BZDTrwXqJg--rDVV0aalSMJJLzgXjIP03X_SfVoyVBJUHcctIT1HoPp0VvnoEvzt75SDkbM6hZRdVlH7IilGcu0t-a-35Npb8tJb9C-EvcUB</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Kopalli, Spandana Rajendra</creator><creator>Cha, Kyu-Min</creator><creator>Cho, Jae Youl</creator><creator>Kim, Si-Kwan</creator><creator>Koppula, Sushruta</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3379-4797</orcidid><orcidid>https://orcid.org/0000-0001-8141-9927</orcidid><orcidid>https://orcid.org/0000-0003-2361-5748</orcidid><orcidid>https://orcid.org/0000-0003-3237-3015</orcidid><orcidid>https://orcid.org/0000-0002-8597-7763</orcidid></search><sort><creationdate>20221201</creationdate><title>Cordycepin mitigates spermatogenic and redox related expression in H2O2-exposed Leydig cells and regulates testicular oxidative apoptotic signalling in aged rats</title><author>Kopalli, Spandana Rajendra ; Cha, Kyu-Min ; Cho, Jae Youl ; Kim, Si-Kwan ; Koppula, Sushruta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d394t-207066af6a16ed38fce5249e5c9d24d8c6c1ef5bd9eb5fb0bb1b4445d1813c5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aging</topic><topic>Apoptosis</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>catalase</topic><topic>Cell viability</topic><topic>Cordycepin</topic><topic>Enzyme immunoassay</topic><topic>Enzymes</topic><topic>glutathione</topic><topic>Glutathione peroxidase</topic><topic>Glutathione transferase</topic><topic>Hydrogen peroxide</topic><topic>Infertility</topic><topic>Inhibin</topic><topic>Leydig cells</topic><topic>lipid peroxidation</topic><topic>Nectin</topic><topic>Oxidative stress</topic><topic>p53 Protein</topic><topic>Peroxiredoxin</topic><topic>Spermatogenesis</topic><topic>spermatogenic factors</topic><topic>Testosterone</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kopalli, Spandana Rajendra</creatorcontrib><creatorcontrib>Cha, Kyu-Min</creatorcontrib><creatorcontrib>Cho, Jae Youl</creatorcontrib><creatorcontrib>Kim, Si-Kwan</creatorcontrib><creatorcontrib>Koppula, Sushruta</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Pharmaceutical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kopalli, Spandana Rajendra</au><au>Cha, Kyu-Min</au><au>Cho, Jae Youl</au><au>Kim, Si-Kwan</au><au>Koppula, Sushruta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cordycepin mitigates spermatogenic and redox related expression in H2O2-exposed Leydig cells and regulates testicular oxidative apoptotic signalling in aged rats</atitle><jtitle>Pharmaceutical biology</jtitle><date>2022-12-01</date><risdate>2022</risdate><volume>60</volume><issue>1</issue><spage>404</spage><epage>416</epage><pages>404-416</pages><issn>1388-0209</issn><eissn>1744-5116</eissn><abstract>Cordycepin (COR), from Cordyceps militaris L., (Cordycipitaceae), is a valuable agent with immense health benefits.
The protective effects of COR in ageing-associated oxidative and apoptosis events in vivo and hydrogen peroxide (H
2
O
2
)-exposed spermatogenesis gene alterations in TM3 Leydig cells was investigated.
Male Sprague-Dawley rats were divided into young control (YC), aged control (AC) and COR treated (COR-20) aged groups. COR-20 group received daily doses of COR (20 mg/kg) for 6 months. Cell viability and hormone levels were analysed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and enzyme immunoassay kits with COR treated at 1, 5, and 10 μg/mL. Oxidative enzymes, spermatogenic, and apoptotic expression in testis tissues were evaluated by Western blotting and real-time RT-PCR.
COR treatment (1, 5, and 10 μg/mL) significantly (p < 0.05 ∼ p < 0.001) inhibited the H
2
O
2
-induced decrease in the percentage of viable cells (from 63.27% to 71.25%, 85.67% and 93.97%, respectively), and reduced the malondialdehyde (MDA) content (from 4.28 to 3.98, 3.14 and 1.78 nM MDA/mg protein, respectively). Further, the decreased antioxidant enzymes (glutathione-S-transferase mu5, glutathione peroxidase 4 and peroxiredoxin 3), spermatogenesis-related factors (nectin-2 and inhibin-α) and testosterone levels in H
2
O
2
-exposed TM3 cells were significantly (p < 0.05 ∼ p < 0.001) ameliorated by COR. In aged rats, COR (20 mg/kg) restored the altered enzymatic and non-enzymatic antioxidative status and attenuated the apoptotic p53 and Bax/Bcl-2 expression significantly (p < 0.05).
COR might be developed as a potential agent against ageing-associated and oxidative stress-induced male infertility.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><pmid>35175170</pmid><doi>10.1080/13880209.2022.2033275</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-3379-4797</orcidid><orcidid>https://orcid.org/0000-0001-8141-9927</orcidid><orcidid>https://orcid.org/0000-0003-2361-5748</orcidid><orcidid>https://orcid.org/0000-0003-3237-3015</orcidid><orcidid>https://orcid.org/0000-0002-8597-7763</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1388-0209 |
| ispartof | Pharmaceutical biology, 2022-12, Vol.60 (1), p.404-416 |
| issn | 1388-0209 1744-5116 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8863333 |
| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Aging Apoptosis BAX protein Bcl-2 protein catalase Cell viability Cordycepin Enzyme immunoassay Enzymes glutathione Glutathione peroxidase Glutathione transferase Hydrogen peroxide Infertility Inhibin Leydig cells lipid peroxidation Nectin Oxidative stress p53 Protein Peroxiredoxin Spermatogenesis spermatogenic factors Testosterone Western blotting |
| title | Cordycepin mitigates spermatogenic and redox related expression in H2O2-exposed Leydig cells and regulates testicular oxidative apoptotic signalling in aged rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T01%3A00%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cordycepin%20mitigates%20spermatogenic%20and%20redox%20related%20expression%20in%20H2O2-exposed%20Leydig%20cells%20and%20regulates%20testicular%20oxidative%20apoptotic%20signalling%20in%20aged%20rats&rft.jtitle=Pharmaceutical%20biology&rft.au=Kopalli,%20Spandana%20Rajendra&rft.date=2022-12-01&rft.volume=60&rft.issue=1&rft.spage=404&rft.epage=416&rft.pages=404-416&rft.issn=1388-0209&rft.eissn=1744-5116&rft_id=info:doi/10.1080/13880209.2022.2033275&rft_dat=%3Cproquest_pubme%3E2761422960%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2761422960&rft_id=info:pmid/35175170&rft_doaj_id=oai_doaj_org_article_fa228af70f1942f2868d632c31b4983e&rfr_iscdi=true |