Isothermal amplification-assisted diagnostics for COVID-19
The scenery of molecular diagnostics for infectious diseases is rapidly evolving to respond to the COVID-19 epidemic. The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to address the disease spreading around the wor...
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| Veröffentlicht in: | Biosensors & bioelectronics 2022-06, Vol.205, p.114101-114101, Article 114101 |
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| container_end_page | 114101 |
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| container_title | Biosensors & bioelectronics |
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| creator | De Felice, Mariarita De Falco, Mariarosaria Zappi, Daniele Antonacci, Amina Scognamiglio, Viviana |
| description | The scenery of molecular diagnostics for infectious diseases is rapidly evolving to respond to the COVID-19 epidemic. The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to address the disease spreading around the world. Emerging diagnostic devices combine the latest trends in isothermal amplification methods for nucleic acids with state-of-the-art biosensing systems, intending to bypass roadblocks encountered in the last 2 years of the pandemic. Isothermal nucleic acid amplification is a simple procedure that quickly and efficiently accumulates nucleic acid sequences at a constant temperature, without the need for sophisticated equipment. The integration of isothermal amplification into portable biosensing devices confers high sensitivity and improves screening at the point of need in low-resource settings. This review reports the latest trends reached in this field with the latest examples of isothermal amplification-powered biosensors for detecting SARS-CoV-2, with different configurations, as well as their intrinsic advantages and disadvantages. |
| doi_str_mv | 10.1016/j.bios.2022.114101 |
| format | Article |
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The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to address the disease spreading around the world. Emerging diagnostic devices combine the latest trends in isothermal amplification methods for nucleic acids with state-of-the-art biosensing systems, intending to bypass roadblocks encountered in the last 2 years of the pandemic. Isothermal nucleic acid amplification is a simple procedure that quickly and efficiently accumulates nucleic acid sequences at a constant temperature, without the need for sophisticated equipment. The integration of isothermal amplification into portable biosensing devices confers high sensitivity and improves screening at the point of need in low-resource settings. 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| ispartof | Biosensors & bioelectronics, 2022-06, Vol.205, p.114101-114101, Article 114101 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Biosensing Techniques Biosensors COVID-19 - diagnosis High throughput Humans Isothermal amplification Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods Nucleic Acids RNA, Viral SARS-CoV-2 - genetics SARS-CoV-2 diagnostics Sensitive and specific screening Sensitivity and Specificity |
| title | Isothermal amplification-assisted diagnostics for COVID-19 |
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