Rapid detection of SARS-CoV-2 variants using allele-specific PCR
Tracking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants through whole genome sequencing (WGS) is vital for effective infection control and prevention (IPC) measures, but can be time-consuming and resource-heavy. We describe an in-house validation of an allele-specific polymera...
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| Veröffentlicht in: | Journal of virological methods 2022-05, Vol.303, p.114497-114497, Article 114497 |
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| container_title | Journal of virological methods |
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| creator | Brito-Mutunayagam, Sanjita Maloney, Daniel McAllister, Gina Dewar, Rebecca McHugh, Martin Templeton, Kate |
| description | Tracking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants through whole genome sequencing (WGS) is vital for effective infection control and prevention (IPC) measures, but can be time-consuming and resource-heavy. We describe an in-house validation of an allele-specific polymerase chain reaction (ASP) variant assay to detect variants of concern (VOC). ASP sensitivity for detecting Delta, Alpha and Beta was 99.45 %, 100 %, and 66.67 %, respectively, compared with WGS. Specificity was 100 % in detecting all three VOC. ASP generated results 1.3 days faster compared with WGS. These findings suggest using variant assays such as ASP may enhance epidemiological surveillance and IPC measures. |
| doi_str_mv | 10.1016/j.jviromet.2022.114497 |
| format | Article |
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We describe an in-house validation of an allele-specific polymerase chain reaction (ASP) variant assay to detect variants of concern (VOC). ASP sensitivity for detecting Delta, Alpha and Beta was 99.45 %, 100 %, and 66.67 %, respectively, compared with WGS. Specificity was 100 % in detecting all three VOC. ASP generated results 1.3 days faster compared with WGS. 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| ispartof | Journal of virological methods, 2022-05, Vol.303, p.114497-114497, Article 114497 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Allele-specific PCR Alleles COVID-19 - diagnosis Humans Mutation Polymerase Chain Reaction SARS-CoV-2 SARS-CoV-2 - genetics Variant assay Variants of concerns Whole genome sequencing |
| title | Rapid detection of SARS-CoV-2 variants using allele-specific PCR |
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