HPV16 AND EXPRESSION OF PROTEIN P16INK4A AND E7 ONCOPROTEIN IN COLORECTAL CARCINOMA
Human papillomavirus (HPV) is the agent of the most prevalent sexually transmitted diseases in the world associated with cervix and anal canal cancer. The action of HPV on colorectal carcinogenesis is not yet established. This research aimed to study the possible correlation between the presence of...
Gespeichert in:
| Veröffentlicht in: | Arquivos brasileiros de cirurgia digestiva : ABCD 2022, Vol.34 (4), p.e1637 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng ; por |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 4 |
| container_start_page | e1637 |
| container_title | Arquivos brasileiros de cirurgia digestiva : ABCD |
| container_volume | 34 |
| creator | Picanço-Junior, Olavo Magalhães Theodoro, Thérèse Rachell Albuquerque, Paulo José de Brito Silva Pinheiro, Rodrigo Nascimento Waisberg, Jaques |
| description | Human papillomavirus (HPV) is the agent of the most prevalent sexually transmitted diseases in the world associated with cervix and anal canal cancer. The action of HPV on colorectal carcinogenesis is not yet established. This research aimed to study the possible correlation between the presence of HPV16 and the gene expression of p16INK4a protein and HPV E7 oncoprotein and their levels in colorectal carcinoma tissue.
A retrospective case-control study of 79 patients with colorectal carcinoma was divided into two groups: HPV-positive and HPV-negative. The polymerase chain reaction was performed, in addition to dot-blot hybridization for HPV16 and HPV18. Colorectal tissue samples were also subjected to immunohistochemical study to assess the tissue level of E7 and p16INK4a proteins.
HPV was identified in 36 (45.6%) cases. There was no significant difference between groups regarding gender (p=0.056), age (p=0.1), colic and/or rectal location (0.098), and presence of HPV. Gene expression of HPV E7 oncoprotein was present in 3.12% of cases (p=0.9), and p16INK4a protein expression was observed in 46.3% (p=0.27) of those selected with HPV detection.
Gene expression and tissue levels of E7 oncoprotein and p16INK4a protein found in HPV-positive patients suggest the absence of HPV16 oncogenic activity in colorectal carcinoma. |
| doi_str_mv | 10.1590/0102-672020210002e1637 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8846486</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>35107499</sourcerecordid><originalsourceid>FETCH-LOGICAL-p196t-58c44c6b389749ce8c511799018fa23365cf9f657f709f5c5417b201e8f4a6ce3</originalsourceid><addsrcrecordid>eNpVkNtKw0AQhhdRbKl9hbIvEN3Jnm-EsKY2GLMhqeJdSNddrfREWwXf3kBtUWZgLv5vPoZBaATkGrgmNwRIHAkZk66AEBJ7EFSeoX5MQUaCxuIc9U9QDw13u48OI1QTpuUl6lEORDKt-6ielM8gcFLc4fSlrNK6zmyB7RiXlZ2mWYFLEFnxwJIDIrEtjD1mXRub2yo10yTHJqlMVtjH5ApdhHax88PfOUBP43RqJlFu7zOT5NEGtNhHXDnGnJhRpbtbnFeOA0itCajQxpQK7oIOgssgiQ7ccQZyFhPwKrBWOE8H6Pbg3XzOlv7V-dV-2y6azXa-bLffzbqdN_-T1fy9eVt_NUoxwZToBKO_gtPm8T30BxBvYaM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>HPV16 AND EXPRESSION OF PROTEIN P16INK4A AND E7 ONCOPROTEIN IN COLORECTAL CARCINOMA</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Picanço-Junior, Olavo Magalhães ; Theodoro, Thérèse Rachell ; Albuquerque, Paulo José de Brito Silva ; Pinheiro, Rodrigo Nascimento ; Waisberg, Jaques</creator><creatorcontrib>Picanço-Junior, Olavo Magalhães ; Theodoro, Thérèse Rachell ; Albuquerque, Paulo José de Brito Silva ; Pinheiro, Rodrigo Nascimento ; Waisberg, Jaques</creatorcontrib><description>Human papillomavirus (HPV) is the agent of the most prevalent sexually transmitted diseases in the world associated with cervix and anal canal cancer. The action of HPV on colorectal carcinogenesis is not yet established. This research aimed to study the possible correlation between the presence of HPV16 and the gene expression of p16INK4a protein and HPV E7 oncoprotein and their levels in colorectal carcinoma tissue.
A retrospective case-control study of 79 patients with colorectal carcinoma was divided into two groups: HPV-positive and HPV-negative. The polymerase chain reaction was performed, in addition to dot-blot hybridization for HPV16 and HPV18. Colorectal tissue samples were also subjected to immunohistochemical study to assess the tissue level of E7 and p16INK4a proteins.
HPV was identified in 36 (45.6%) cases. There was no significant difference between groups regarding gender (p=0.056), age (p=0.1), colic and/or rectal location (0.098), and presence of HPV. Gene expression of HPV E7 oncoprotein was present in 3.12% of cases (p=0.9), and p16INK4a protein expression was observed in 46.3% (p=0.27) of those selected with HPV detection.
Gene expression and tissue levels of E7 oncoprotein and p16INK4a protein found in HPV-positive patients suggest the absence of HPV16 oncogenic activity in colorectal carcinoma.</description><identifier>ISSN: 0102-6720</identifier><identifier>EISSN: 2317-6326</identifier><identifier>DOI: 10.1590/0102-672020210002e1637</identifier><identifier>PMID: 35107499</identifier><language>eng ; por</language><publisher>Brazil: Colégio Brasileiro de Cirurgia Digestiva</publisher><subject>Artigo Original ; Case-Control Studies ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - virology ; Cyclin-Dependent Kinase Inhibitor p16 - genetics ; DNA, Viral ; Female ; Human papillomavirus 16 - genetics ; Humans ; Papillomavirus E7 Proteins - genetics ; Papillomavirus Infections - genetics ; Retrospective Studies</subject><ispartof>Arquivos brasileiros de cirurgia digestiva : ABCD, 2022, Vol.34 (4), p.e1637</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-0621-5243 ; 0000-0003-4972-3448 ; 0000-0003-2775-8068 ; 0000-0002-2715-7628 ; 0000-0003-0498-2622</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846486/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846486/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35107499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Picanço-Junior, Olavo Magalhães</creatorcontrib><creatorcontrib>Theodoro, Thérèse Rachell</creatorcontrib><creatorcontrib>Albuquerque, Paulo José de Brito Silva</creatorcontrib><creatorcontrib>Pinheiro, Rodrigo Nascimento</creatorcontrib><creatorcontrib>Waisberg, Jaques</creatorcontrib><title>HPV16 AND EXPRESSION OF PROTEIN P16INK4A AND E7 ONCOPROTEIN IN COLORECTAL CARCINOMA</title><title>Arquivos brasileiros de cirurgia digestiva : ABCD</title><addtitle>Arq Bras Cir Dig</addtitle><description>Human papillomavirus (HPV) is the agent of the most prevalent sexually transmitted diseases in the world associated with cervix and anal canal cancer. The action of HPV on colorectal carcinogenesis is not yet established. This research aimed to study the possible correlation between the presence of HPV16 and the gene expression of p16INK4a protein and HPV E7 oncoprotein and their levels in colorectal carcinoma tissue.
A retrospective case-control study of 79 patients with colorectal carcinoma was divided into two groups: HPV-positive and HPV-negative. The polymerase chain reaction was performed, in addition to dot-blot hybridization for HPV16 and HPV18. Colorectal tissue samples were also subjected to immunohistochemical study to assess the tissue level of E7 and p16INK4a proteins.
HPV was identified in 36 (45.6%) cases. There was no significant difference between groups regarding gender (p=0.056), age (p=0.1), colic and/or rectal location (0.098), and presence of HPV. Gene expression of HPV E7 oncoprotein was present in 3.12% of cases (p=0.9), and p16INK4a protein expression was observed in 46.3% (p=0.27) of those selected with HPV detection.
Gene expression and tissue levels of E7 oncoprotein and p16INK4a protein found in HPV-positive patients suggest the absence of HPV16 oncogenic activity in colorectal carcinoma.</description><subject>Artigo Original</subject><subject>Case-Control Studies</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - virology</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - genetics</subject><subject>DNA, Viral</subject><subject>Female</subject><subject>Human papillomavirus 16 - genetics</subject><subject>Humans</subject><subject>Papillomavirus E7 Proteins - genetics</subject><subject>Papillomavirus Infections - genetics</subject><subject>Retrospective Studies</subject><issn>0102-6720</issn><issn>2317-6326</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkNtKw0AQhhdRbKl9hbIvEN3Jnm-EsKY2GLMhqeJdSNddrfREWwXf3kBtUWZgLv5vPoZBaATkGrgmNwRIHAkZk66AEBJ7EFSeoX5MQUaCxuIc9U9QDw13u48OI1QTpuUl6lEORDKt-6ielM8gcFLc4fSlrNK6zmyB7RiXlZ2mWYFLEFnxwJIDIrEtjD1mXRub2yo10yTHJqlMVtjH5ApdhHax88PfOUBP43RqJlFu7zOT5NEGtNhHXDnGnJhRpbtbnFeOA0itCajQxpQK7oIOgssgiQ7ccQZyFhPwKrBWOE8H6Pbg3XzOlv7V-dV-2y6azXa-bLffzbqdN_-T1fy9eVt_NUoxwZToBKO_gtPm8T30BxBvYaM</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Picanço-Junior, Olavo Magalhães</creator><creator>Theodoro, Thérèse Rachell</creator><creator>Albuquerque, Paulo José de Brito Silva</creator><creator>Pinheiro, Rodrigo Nascimento</creator><creator>Waisberg, Jaques</creator><general>Colégio Brasileiro de Cirurgia Digestiva</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0621-5243</orcidid><orcidid>https://orcid.org/0000-0003-4972-3448</orcidid><orcidid>https://orcid.org/0000-0003-2775-8068</orcidid><orcidid>https://orcid.org/0000-0002-2715-7628</orcidid><orcidid>https://orcid.org/0000-0003-0498-2622</orcidid></search><sort><creationdate>2022</creationdate><title>HPV16 AND EXPRESSION OF PROTEIN P16INK4A AND E7 ONCOPROTEIN IN COLORECTAL CARCINOMA</title><author>Picanço-Junior, Olavo Magalhães ; Theodoro, Thérèse Rachell ; Albuquerque, Paulo José de Brito Silva ; Pinheiro, Rodrigo Nascimento ; Waisberg, Jaques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p196t-58c44c6b389749ce8c511799018fa23365cf9f657f709f5c5417b201e8f4a6ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; por</language><creationdate>2022</creationdate><topic>Artigo Original</topic><topic>Case-Control Studies</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - virology</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - genetics</topic><topic>DNA, Viral</topic><topic>Female</topic><topic>Human papillomavirus 16 - genetics</topic><topic>Humans</topic><topic>Papillomavirus E7 Proteins - genetics</topic><topic>Papillomavirus Infections - genetics</topic><topic>Retrospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Picanço-Junior, Olavo Magalhães</creatorcontrib><creatorcontrib>Theodoro, Thérèse Rachell</creatorcontrib><creatorcontrib>Albuquerque, Paulo José de Brito Silva</creatorcontrib><creatorcontrib>Pinheiro, Rodrigo Nascimento</creatorcontrib><creatorcontrib>Waisberg, Jaques</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Arquivos brasileiros de cirurgia digestiva : ABCD</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Picanço-Junior, Olavo Magalhães</au><au>Theodoro, Thérèse Rachell</au><au>Albuquerque, Paulo José de Brito Silva</au><au>Pinheiro, Rodrigo Nascimento</au><au>Waisberg, Jaques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPV16 AND EXPRESSION OF PROTEIN P16INK4A AND E7 ONCOPROTEIN IN COLORECTAL CARCINOMA</atitle><jtitle>Arquivos brasileiros de cirurgia digestiva : ABCD</jtitle><addtitle>Arq Bras Cir Dig</addtitle><date>2022</date><risdate>2022</risdate><volume>34</volume><issue>4</issue><spage>e1637</spage><pages>e1637-</pages><issn>0102-6720</issn><eissn>2317-6326</eissn><abstract>Human papillomavirus (HPV) is the agent of the most prevalent sexually transmitted diseases in the world associated with cervix and anal canal cancer. The action of HPV on colorectal carcinogenesis is not yet established. This research aimed to study the possible correlation between the presence of HPV16 and the gene expression of p16INK4a protein and HPV E7 oncoprotein and their levels in colorectal carcinoma tissue.
A retrospective case-control study of 79 patients with colorectal carcinoma was divided into two groups: HPV-positive and HPV-negative. The polymerase chain reaction was performed, in addition to dot-blot hybridization for HPV16 and HPV18. Colorectal tissue samples were also subjected to immunohistochemical study to assess the tissue level of E7 and p16INK4a proteins.
HPV was identified in 36 (45.6%) cases. There was no significant difference between groups regarding gender (p=0.056), age (p=0.1), colic and/or rectal location (0.098), and presence of HPV. Gene expression of HPV E7 oncoprotein was present in 3.12% of cases (p=0.9), and p16INK4a protein expression was observed in 46.3% (p=0.27) of those selected with HPV detection.
Gene expression and tissue levels of E7 oncoprotein and p16INK4a protein found in HPV-positive patients suggest the absence of HPV16 oncogenic activity in colorectal carcinoma.</abstract><cop>Brazil</cop><pub>Colégio Brasileiro de Cirurgia Digestiva</pub><pmid>35107499</pmid><doi>10.1590/0102-672020210002e1637</doi><orcidid>https://orcid.org/0000-0002-0621-5243</orcidid><orcidid>https://orcid.org/0000-0003-4972-3448</orcidid><orcidid>https://orcid.org/0000-0003-2775-8068</orcidid><orcidid>https://orcid.org/0000-0002-2715-7628</orcidid><orcidid>https://orcid.org/0000-0003-0498-2622</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0102-6720 |
| ispartof | Arquivos brasileiros de cirurgia digestiva : ABCD, 2022, Vol.34 (4), p.e1637 |
| issn | 0102-6720 2317-6326 |
| language | eng ; por |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8846486 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Artigo Original Case-Control Studies Colorectal Neoplasms - genetics Colorectal Neoplasms - virology Cyclin-Dependent Kinase Inhibitor p16 - genetics DNA, Viral Female Human papillomavirus 16 - genetics Humans Papillomavirus E7 Proteins - genetics Papillomavirus Infections - genetics Retrospective Studies |
| title | HPV16 AND EXPRESSION OF PROTEIN P16INK4A AND E7 ONCOPROTEIN IN COLORECTAL CARCINOMA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T02%3A16%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HPV16%20AND%20EXPRESSION%20OF%20PROTEIN%20P16INK4A%20AND%20E7%20ONCOPROTEIN%20IN%20COLORECTAL%20CARCINOMA&rft.jtitle=Arquivos%20brasileiros%20de%20cirurgia%20digestiva%20:%20ABCD&rft.au=Pican%C3%A7o-Junior,%20Olavo%20Magalh%C3%A3es&rft.date=2022&rft.volume=34&rft.issue=4&rft.spage=e1637&rft.pages=e1637-&rft.issn=0102-6720&rft.eissn=2317-6326&rft_id=info:doi/10.1590/0102-672020210002e1637&rft_dat=%3Cpubmed%3E35107499%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/35107499&rfr_iscdi=true |