Copper(II) and silver(I)-1,10-phenanthroline-5,6-dione complexes interact with double-stranded DNA: further evidence of their apparent multi-modal activity towards Pseudomonas aeruginosa
Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione) 2 ]ClO 4 (Ag-phendione) and [Cu(1,10-phenant...
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| Veröffentlicht in: | Journal of biological inorganic chemistry 2022-02, Vol.27 (1), p.201-213 |
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| creator | Galdino, Anna Clara Milesi Viganor, Lívia Pereira, Matheus Mendonça Devereux, Michael McCann, Malachy Branquinha, Marta Helena Molphy, Zara O’Carroll, Sinéad Bain, Conor Menounou, Georgia Kellett, Andrew dos Santos, André Luis Souza |
| description | Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione)
2
]ClO
4
(Ag-phendione) and [Cu(1,10-phenanthroline-5,6-dione)
3
](ClO
4
)
2
.4H
2
O (Cu-phendione), with efficient antimicrobial action against multidrug-resistant species. Herein, we investigated the ability of Ag-phendione and Cu-phendione to bind with double-stranded DNA using a combination of in silico and in vitro approaches. Molecular docking revealed that both phendione derivatives can interact with the DNA by hydrogen bonding, hydrophobic and electrostatic interactions. Cu-phendione exhibited the highest binding affinity to either major (− 7.9 kcal/mol) or minor (− 7.2 kcal/mol) DNA grooves. In vitro competitive quenching assays involving duplex DNA with Hoechst 33258 or ethidium bromide demonstrated that Ag-phendione and Cu-phendione preferentially bind DNA in the minor grooves. The competitive ethidium bromide displacement technique revealed Cu-phendione has a higher binding affinity to DNA (
K
app
= 2.55 × 10
6
M
−1
) than Ag-phendione (
K
app
= 2.79 × 10
5
M
−1
) and phendione (
K
app
= 1.33 × 10
5
M
−1
). Cu-phendione induced topoisomerase I-mediated DNA relaxation of supercoiled plasmid DNA. Moreover, Cu-phendione was able to induce oxidative DNA injuries with the addition of free radical scavengers inhibiting DNA damage. Ag-phendione and Cu-phendione avidly displaced propidium iodide bound to DNA in permeabilized
Pseudomonas aeruginosa
cells in a dose-dependent manner as judged by flow cytometry. The treatment of
P. aeruginosa
with bactericidal concentrations of Cu-phendione (15 µM) induced DNA fragmentation as visualized by either agarose gel or TUNEL assays. Altogether, these results highlight a possible novel DNA-targeted mechanism by which phendione-containing complexes, in part, elicit toxicity toward the multidrug-resistant pathogen
P. aeruginosa
.
Graphical abstract |
| doi_str_mv | 10.1007/s00775-021-01922-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8840922</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2628157469</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-a7ed807b1526f5e6ac237d47eb1d23152e6315810a0aac3a6ab466b45913119e3</originalsourceid><addsrcrecordid>eNp9kk1v1DAQhiMEokvhD3BAlri0Ug3-iO2EA1K15WOlCjjA2XLi2V1XiZ3azpb-tf46XLaUjwMXW5555vWM5q2q55S8ooSo16kcSmDCKCa0ZQzzB9WC1pxhypl6WC1IW7e4YUIdVE9SuiCEcEHF4-qAC0Ikr9WiulmGaYJ4tFodI-MtSm7Y3T6PMT2hBE9b8MbnbQyD84DFicTWBQ-oD-M0wHdIyPkM0fQZXbm8RTbM3QA45VjUwKKzT6dv0HqOeQsRwc5Z8D2gsEYl4CIy02Qi-IzGecgOj8GaARUxt3P5GuVwZaJN6EuC2YYxeJOQgThvnA_JPK0erc2Q4NndfVh9e__u6_IjPv_8YbU8Pcd9reqMjQLbENVRweRagDQ948rWCjpqGS9RkOVsKDHEmJ4babpayq4WLeWUtsAPq7d73WnuRrB9aTeaQU_RjSZe62Cc_jvj3VZvwk43TU3KWorA0Z1ADJczpKxHl3oYBuMhzEkzSRtByuJkQV_-g16EOfoyXqFYQ4WqZVsotqf6GFKKsL5vhhJ9aw29t4Yu1tA_raF5KXrx5xj3Jb-8UAC-B1JJ-Q3E33__R_YHL-bHKA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2628157469</pqid></control><display><type>article</type><title>Copper(II) and silver(I)-1,10-phenanthroline-5,6-dione complexes interact with double-stranded DNA: further evidence of their apparent multi-modal activity towards Pseudomonas aeruginosa</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Galdino, Anna Clara Milesi ; Viganor, Lívia ; Pereira, Matheus Mendonça ; Devereux, Michael ; McCann, Malachy ; Branquinha, Marta Helena ; Molphy, Zara ; O’Carroll, Sinéad ; Bain, Conor ; Menounou, Georgia ; Kellett, Andrew ; dos Santos, André Luis Souza</creator><creatorcontrib>Galdino, Anna Clara Milesi ; Viganor, Lívia ; Pereira, Matheus Mendonça ; Devereux, Michael ; McCann, Malachy ; Branquinha, Marta Helena ; Molphy, Zara ; O’Carroll, Sinéad ; Bain, Conor ; Menounou, Georgia ; Kellett, Andrew ; dos Santos, André Luis Souza</creatorcontrib><description>Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione)
2
]ClO
4
(Ag-phendione) and [Cu(1,10-phenanthroline-5,6-dione)
3
](ClO
4
)
2
.4H
2
O (Cu-phendione), with efficient antimicrobial action against multidrug-resistant species. Herein, we investigated the ability of Ag-phendione and Cu-phendione to bind with double-stranded DNA using a combination of in silico and in vitro approaches. Molecular docking revealed that both phendione derivatives can interact with the DNA by hydrogen bonding, hydrophobic and electrostatic interactions. Cu-phendione exhibited the highest binding affinity to either major (− 7.9 kcal/mol) or minor (− 7.2 kcal/mol) DNA grooves. In vitro competitive quenching assays involving duplex DNA with Hoechst 33258 or ethidium bromide demonstrated that Ag-phendione and Cu-phendione preferentially bind DNA in the minor grooves. The competitive ethidium bromide displacement technique revealed Cu-phendione has a higher binding affinity to DNA (
K
app
= 2.55 × 10
6
M
−1
) than Ag-phendione (
K
app
= 2.79 × 10
5
M
−1
) and phendione (
K
app
= 1.33 × 10
5
M
−1
). Cu-phendione induced topoisomerase I-mediated DNA relaxation of supercoiled plasmid DNA. Moreover, Cu-phendione was able to induce oxidative DNA injuries with the addition of free radical scavengers inhibiting DNA damage. Ag-phendione and Cu-phendione avidly displaced propidium iodide bound to DNA in permeabilized
Pseudomonas aeruginosa
cells in a dose-dependent manner as judged by flow cytometry. The treatment of
P. aeruginosa
with bactericidal concentrations of Cu-phendione (15 µM) induced DNA fragmentation as visualized by either agarose gel or TUNEL assays. Altogether, these results highlight a possible novel DNA-targeted mechanism by which phendione-containing complexes, in part, elicit toxicity toward the multidrug-resistant pathogen
P. aeruginosa
.
Graphical abstract</description><identifier>ISSN: 0949-8257</identifier><identifier>EISSN: 1432-1327</identifier><identifier>DOI: 10.1007/s00775-021-01922-3</identifier><identifier>PMID: 35006347</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Affinity ; Antimicrobial activity ; Antimicrobial agents ; Biochemistry ; Biomedical and Life Sciences ; Coordination Complexes - chemistry ; Coordination Complexes - pharmacology ; Copper ; Copper - chemistry ; Copper - pharmacology ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; DNA damage ; DNA fragmentation ; DNA topoisomerase ; Electrostatic properties ; Ethidium bromide ; Flow cytometry ; Hydrogen bonding ; Hydrophobicity ; Life Sciences ; Microbiology ; Molecular Docking Simulation ; Multidrug resistance ; Original Paper ; Phenanthrolines - chemistry ; Phenanthrolines - pharmacology ; Propidium iodide ; Pseudomonas aeruginosa ; Silver ; Silver - pharmacology ; Toxicity</subject><ispartof>Journal of biological inorganic chemistry, 2022-02, Vol.27 (1), p.201-213</ispartof><rights>The Author(s) 2022</rights><rights>2022. The Author(s).</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-a7ed807b1526f5e6ac237d47eb1d23152e6315810a0aac3a6ab466b45913119e3</citedby><cites>FETCH-LOGICAL-c474t-a7ed807b1526f5e6ac237d47eb1d23152e6315810a0aac3a6ab466b45913119e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00775-021-01922-3$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00775-021-01922-3$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35006347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Galdino, Anna Clara Milesi</creatorcontrib><creatorcontrib>Viganor, Lívia</creatorcontrib><creatorcontrib>Pereira, Matheus Mendonça</creatorcontrib><creatorcontrib>Devereux, Michael</creatorcontrib><creatorcontrib>McCann, Malachy</creatorcontrib><creatorcontrib>Branquinha, Marta Helena</creatorcontrib><creatorcontrib>Molphy, Zara</creatorcontrib><creatorcontrib>O’Carroll, Sinéad</creatorcontrib><creatorcontrib>Bain, Conor</creatorcontrib><creatorcontrib>Menounou, Georgia</creatorcontrib><creatorcontrib>Kellett, Andrew</creatorcontrib><creatorcontrib>dos Santos, André Luis Souza</creatorcontrib><title>Copper(II) and silver(I)-1,10-phenanthroline-5,6-dione complexes interact with double-stranded DNA: further evidence of their apparent multi-modal activity towards Pseudomonas aeruginosa</title><title>Journal of biological inorganic chemistry</title><addtitle>J Biol Inorg Chem</addtitle><addtitle>J Biol Inorg Chem</addtitle><description>Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione)
2
]ClO
4
(Ag-phendione) and [Cu(1,10-phenanthroline-5,6-dione)
3
](ClO
4
)
2
.4H
2
O (Cu-phendione), with efficient antimicrobial action against multidrug-resistant species. Herein, we investigated the ability of Ag-phendione and Cu-phendione to bind with double-stranded DNA using a combination of in silico and in vitro approaches. Molecular docking revealed that both phendione derivatives can interact with the DNA by hydrogen bonding, hydrophobic and electrostatic interactions. Cu-phendione exhibited the highest binding affinity to either major (− 7.9 kcal/mol) or minor (− 7.2 kcal/mol) DNA grooves. In vitro competitive quenching assays involving duplex DNA with Hoechst 33258 or ethidium bromide demonstrated that Ag-phendione and Cu-phendione preferentially bind DNA in the minor grooves. The competitive ethidium bromide displacement technique revealed Cu-phendione has a higher binding affinity to DNA (
K
app
= 2.55 × 10
6
M
−1
) than Ag-phendione (
K
app
= 2.79 × 10
5
M
−1
) and phendione (
K
app
= 1.33 × 10
5
M
−1
). Cu-phendione induced topoisomerase I-mediated DNA relaxation of supercoiled plasmid DNA. Moreover, Cu-phendione was able to induce oxidative DNA injuries with the addition of free radical scavengers inhibiting DNA damage. Ag-phendione and Cu-phendione avidly displaced propidium iodide bound to DNA in permeabilized
Pseudomonas aeruginosa
cells in a dose-dependent manner as judged by flow cytometry. The treatment of
P. aeruginosa
with bactericidal concentrations of Cu-phendione (15 µM) induced DNA fragmentation as visualized by either agarose gel or TUNEL assays. Altogether, these results highlight a possible novel DNA-targeted mechanism by which phendione-containing complexes, in part, elicit toxicity toward the multidrug-resistant pathogen
P. aeruginosa
.
Graphical abstract</description><subject>Affinity</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Coordination Complexes - chemistry</subject><subject>Coordination Complexes - pharmacology</subject><subject>Copper</subject><subject>Copper - chemistry</subject><subject>Copper - pharmacology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA damage</subject><subject>DNA fragmentation</subject><subject>DNA topoisomerase</subject><subject>Electrostatic properties</subject><subject>Ethidium bromide</subject><subject>Flow cytometry</subject><subject>Hydrogen bonding</subject><subject>Hydrophobicity</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Docking Simulation</subject><subject>Multidrug resistance</subject><subject>Original Paper</subject><subject>Phenanthrolines - chemistry</subject><subject>Phenanthrolines - pharmacology</subject><subject>Propidium iodide</subject><subject>Pseudomonas aeruginosa</subject><subject>Silver</subject><subject>Silver - pharmacology</subject><subject>Toxicity</subject><issn>0949-8257</issn><issn>1432-1327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kk1v1DAQhiMEokvhD3BAlri0Ug3-iO2EA1K15WOlCjjA2XLi2V1XiZ3azpb-tf46XLaUjwMXW5555vWM5q2q55S8ooSo16kcSmDCKCa0ZQzzB9WC1pxhypl6WC1IW7e4YUIdVE9SuiCEcEHF4-qAC0Ikr9WiulmGaYJ4tFodI-MtSm7Y3T6PMT2hBE9b8MbnbQyD84DFicTWBQ-oD-M0wHdIyPkM0fQZXbm8RTbM3QA45VjUwKKzT6dv0HqOeQsRwc5Z8D2gsEYl4CIy02Qi-IzGecgOj8GaARUxt3P5GuVwZaJN6EuC2YYxeJOQgThvnA_JPK0erc2Q4NndfVh9e__u6_IjPv_8YbU8Pcd9reqMjQLbENVRweRagDQ948rWCjpqGS9RkOVsKDHEmJ4babpayq4WLeWUtsAPq7d73WnuRrB9aTeaQU_RjSZe62Cc_jvj3VZvwk43TU3KWorA0Z1ADJczpKxHl3oYBuMhzEkzSRtByuJkQV_-g16EOfoyXqFYQ4WqZVsotqf6GFKKsL5vhhJ9aw29t4Yu1tA_raF5KXrx5xj3Jb-8UAC-B1JJ-Q3E33__R_YHL-bHKA</recordid><startdate>20220201</startdate><enddate>20220201</enddate><creator>Galdino, Anna Clara Milesi</creator><creator>Viganor, Lívia</creator><creator>Pereira, Matheus Mendonça</creator><creator>Devereux, Michael</creator><creator>McCann, Malachy</creator><creator>Branquinha, Marta Helena</creator><creator>Molphy, Zara</creator><creator>O’Carroll, Sinéad</creator><creator>Bain, Conor</creator><creator>Menounou, Georgia</creator><creator>Kellett, Andrew</creator><creator>dos Santos, André Luis Souza</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220201</creationdate><title>Copper(II) and silver(I)-1,10-phenanthroline-5,6-dione complexes interact with double-stranded DNA: further evidence of their apparent multi-modal activity towards Pseudomonas aeruginosa</title><author>Galdino, Anna Clara Milesi ; Viganor, Lívia ; Pereira, Matheus Mendonça ; Devereux, Michael ; McCann, Malachy ; Branquinha, Marta Helena ; Molphy, Zara ; O’Carroll, Sinéad ; Bain, Conor ; Menounou, Georgia ; Kellett, Andrew ; dos Santos, André Luis Souza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-a7ed807b1526f5e6ac237d47eb1d23152e6315810a0aac3a6ab466b45913119e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Affinity</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Coordination Complexes - chemistry</topic><topic>Coordination Complexes - pharmacology</topic><topic>Copper</topic><topic>Copper - chemistry</topic><topic>Copper - pharmacology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA damage</topic><topic>DNA fragmentation</topic><topic>DNA topoisomerase</topic><topic>Electrostatic properties</topic><topic>Ethidium bromide</topic><topic>Flow cytometry</topic><topic>Hydrogen bonding</topic><topic>Hydrophobicity</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Docking Simulation</topic><topic>Multidrug resistance</topic><topic>Original Paper</topic><topic>Phenanthrolines - chemistry</topic><topic>Phenanthrolines - pharmacology</topic><topic>Propidium iodide</topic><topic>Pseudomonas aeruginosa</topic><topic>Silver</topic><topic>Silver - pharmacology</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galdino, Anna Clara Milesi</creatorcontrib><creatorcontrib>Viganor, Lívia</creatorcontrib><creatorcontrib>Pereira, Matheus Mendonça</creatorcontrib><creatorcontrib>Devereux, Michael</creatorcontrib><creatorcontrib>McCann, Malachy</creatorcontrib><creatorcontrib>Branquinha, Marta Helena</creatorcontrib><creatorcontrib>Molphy, Zara</creatorcontrib><creatorcontrib>O’Carroll, Sinéad</creatorcontrib><creatorcontrib>Bain, Conor</creatorcontrib><creatorcontrib>Menounou, Georgia</creatorcontrib><creatorcontrib>Kellett, Andrew</creatorcontrib><creatorcontrib>dos Santos, André Luis Souza</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biological inorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galdino, Anna Clara Milesi</au><au>Viganor, Lívia</au><au>Pereira, Matheus Mendonça</au><au>Devereux, Michael</au><au>McCann, Malachy</au><au>Branquinha, Marta Helena</au><au>Molphy, Zara</au><au>O’Carroll, Sinéad</au><au>Bain, Conor</au><au>Menounou, Georgia</au><au>Kellett, Andrew</au><au>dos Santos, André Luis Souza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Copper(II) and silver(I)-1,10-phenanthroline-5,6-dione complexes interact with double-stranded DNA: further evidence of their apparent multi-modal activity towards Pseudomonas aeruginosa</atitle><jtitle>Journal of biological inorganic chemistry</jtitle><stitle>J Biol Inorg Chem</stitle><addtitle>J Biol Inorg Chem</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>27</volume><issue>1</issue><spage>201</spage><epage>213</epage><pages>201-213</pages><issn>0949-8257</issn><eissn>1432-1327</eissn><abstract>Tackling microbial resistance requires continuous efforts for the development of new molecules with novel mechanisms of action and potent antimicrobial activity. Our group has previously identified metal-based compounds, [Ag(1,10-phenanthroline-5,6-dione)
2
]ClO
4
(Ag-phendione) and [Cu(1,10-phenanthroline-5,6-dione)
3
](ClO
4
)
2
.4H
2
O (Cu-phendione), with efficient antimicrobial action against multidrug-resistant species. Herein, we investigated the ability of Ag-phendione and Cu-phendione to bind with double-stranded DNA using a combination of in silico and in vitro approaches. Molecular docking revealed that both phendione derivatives can interact with the DNA by hydrogen bonding, hydrophobic and electrostatic interactions. Cu-phendione exhibited the highest binding affinity to either major (− 7.9 kcal/mol) or minor (− 7.2 kcal/mol) DNA grooves. In vitro competitive quenching assays involving duplex DNA with Hoechst 33258 or ethidium bromide demonstrated that Ag-phendione and Cu-phendione preferentially bind DNA in the minor grooves. The competitive ethidium bromide displacement technique revealed Cu-phendione has a higher binding affinity to DNA (
K
app
= 2.55 × 10
6
M
−1
) than Ag-phendione (
K
app
= 2.79 × 10
5
M
−1
) and phendione (
K
app
= 1.33 × 10
5
M
−1
). Cu-phendione induced topoisomerase I-mediated DNA relaxation of supercoiled plasmid DNA. Moreover, Cu-phendione was able to induce oxidative DNA injuries with the addition of free radical scavengers inhibiting DNA damage. Ag-phendione and Cu-phendione avidly displaced propidium iodide bound to DNA in permeabilized
Pseudomonas aeruginosa
cells in a dose-dependent manner as judged by flow cytometry. The treatment of
P. aeruginosa
with bactericidal concentrations of Cu-phendione (15 µM) induced DNA fragmentation as visualized by either agarose gel or TUNEL assays. Altogether, these results highlight a possible novel DNA-targeted mechanism by which phendione-containing complexes, in part, elicit toxicity toward the multidrug-resistant pathogen
P. aeruginosa
.
Graphical abstract</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>35006347</pmid><doi>10.1007/s00775-021-01922-3</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0949-8257 |
| ispartof | Journal of biological inorganic chemistry, 2022-02, Vol.27 (1), p.201-213 |
| issn | 0949-8257 1432-1327 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8840922 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Affinity Antimicrobial activity Antimicrobial agents Biochemistry Biomedical and Life Sciences Coordination Complexes - chemistry Coordination Complexes - pharmacology Copper Copper - chemistry Copper - pharmacology Deoxyribonucleic acid DNA DNA - chemistry DNA damage DNA fragmentation DNA topoisomerase Electrostatic properties Ethidium bromide Flow cytometry Hydrogen bonding Hydrophobicity Life Sciences Microbiology Molecular Docking Simulation Multidrug resistance Original Paper Phenanthrolines - chemistry Phenanthrolines - pharmacology Propidium iodide Pseudomonas aeruginosa Silver Silver - pharmacology Toxicity |
| title | Copper(II) and silver(I)-1,10-phenanthroline-5,6-dione complexes interact with double-stranded DNA: further evidence of their apparent multi-modal activity towards Pseudomonas aeruginosa |
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