Exogenous chalcone synthase expression in developing poplar xylem incorporates naringenin into lignins
Lignin, a polyphenolic polymer, is a major chemical constituent of the cell walls of terrestrial plants. The biosynthesis of lignin is a highly plastic process, as highlighted by an increasing number of noncanonical monomers that have been successfully identified in an array of plants. Here, we engi...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2022-02, Vol.188 (2), p.984-996 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Mahon, Elizabeth L de Vries, Lisanne Jang, Soo-Kyeong Middar, Sandeep Kim, Hoon Unda, Faride Ralph, John Mansfield, Shawn D |
| description | Lignin, a polyphenolic polymer, is a major chemical constituent of the cell walls of terrestrial plants. The biosynthesis of lignin is a highly plastic process, as highlighted by an increasing number of noncanonical monomers that have been successfully identified in an array of plants. Here, we engineered hybrid poplar (Populus alba x grandidentata) to express chalcone synthase 3 (MdCHS3) derived from apple (Malus domestica) in lignifying xylem. Transgenic trees displayed an accumulation of the flavonoid naringenin in xylem methanolic extracts not inherently observed in wild-type trees. Nuclear magnetic resonance analysis revealed the presence of naringenin in the extract-free, cellulase-treated xylem lignin of MdCHS3-poplar, indicating the incorporation of this flavonoid-derived compound into poplar secondary cell wall lignins. The transgenic trees also displayed lower total cell wall lignin content and increased cell wall carbohydrate content and performed significantly better in limited saccharification assays than their wild-type counterparts. |
| doi_str_mv | 10.1093/plphys/kiab499 |
| format | Article |
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The biosynthesis of lignin is a highly plastic process, as highlighted by an increasing number of noncanonical monomers that have been successfully identified in an array of plants. Here, we engineered hybrid poplar (Populus alba x grandidentata) to express chalcone synthase 3 (MdCHS3) derived from apple (Malus domestica) in lignifying xylem. Transgenic trees displayed an accumulation of the flavonoid naringenin in xylem methanolic extracts not inherently observed in wild-type trees. Nuclear magnetic resonance analysis revealed the presence of naringenin in the extract-free, cellulase-treated xylem lignin of MdCHS3-poplar, indicating the incorporation of this flavonoid-derived compound into poplar secondary cell wall lignins. The transgenic trees also displayed lower total cell wall lignin content and increased cell wall carbohydrate content and performed significantly better in limited saccharification assays than their wild-type counterparts.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1093/plphys/kiab499</identifier><identifier>PMID: 34718804</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Acyltransferases - genetics ; Acyltransferases - metabolism ; BASIC BIOLOGICAL SCIENCES ; Crops, Agricultural - genetics ; Crops, Agricultural - metabolism ; Flavanones - genetics ; Flavanones - metabolism ; Gene Expression Regulation, Plant ; Genes, Plant ; Lignin - biosynthesis ; Lignin - genetics ; Malus - genetics ; Malus - metabolism ; Plants, Genetically Modified - metabolism ; Populus - genetics ; Populus - metabolism ; Regular Issue Content ; Xylem - genetics ; Xylem - metabolism</subject><ispartof>Plant physiology (Bethesda), 2022-02, Vol.188 (2), p.984-996</ispartof><rights>The Author(s) 2021. 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The transgenic trees also displayed lower total cell wall lignin content and increased cell wall carbohydrate content and performed significantly better in limited saccharification assays than their wild-type counterparts.</description><subject>Acyltransferases - genetics</subject><subject>Acyltransferases - metabolism</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Crops, Agricultural - genetics</subject><subject>Crops, Agricultural - metabolism</subject><subject>Flavanones - genetics</subject><subject>Flavanones - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>Lignin - biosynthesis</subject><subject>Lignin - genetics</subject><subject>Malus - genetics</subject><subject>Malus - metabolism</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Populus - genetics</subject><subject>Populus - metabolism</subject><subject>Regular Issue Content</subject><subject>Xylem - genetics</subject><subject>Xylem - metabolism</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1PWzEQtCqqkoZee0QWJy4Bf708-4KEELSVkHopZ8tx9uW5dWxjOyj59zhKQPS0I-3s7M4OQt8puaJE8evk07gr1_-cWQilPqEJ7TibsU7IEzQhpGEipTpFX0v5SwihnIov6JSLnkpJxAQN99u4ghA3BdvReBsD4LILdTQFMGxThlJcDNgFvIQX8DG5sMIpJm8y3u48rFvLxpxiNhUKDiY3AgS3H6kRe7dquJyhz4PxBb4d6xQ9Pdz_ufs5e_z949fd7ePMCtrX2TDMmeQKlFxaS-i8WQRGOik7kM2tHBY9sWAM6ZdzOyhLGbWsk2BF1_eDBD5FNwfdtFmsYWkh1Gy8TtmtTd7paJz-vxPcqFfxRUvJOt5WTNHFQSCW6nSxroId21cC2KqpFIJw0kiXxy05Pm-gVL12xYL3JkD7pGadoowoSfd6VweqzbGUDMP7LZTofYL6kKA-JtgGzj86eKe_RcZfAdoJnYE</recordid><startdate>20220204</startdate><enddate>20220204</enddate><creator>Mahon, Elizabeth L</creator><creator>de Vries, Lisanne</creator><creator>Jang, Soo-Kyeong</creator><creator>Middar, Sandeep</creator><creator>Kim, Hoon</creator><creator>Unda, Faride</creator><creator>Ralph, John</creator><creator>Mansfield, Shawn D</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6093-4521</orcidid><orcidid>https://orcid.org/0000-0002-3245-9081</orcidid><orcidid>https://orcid.org/0000-0001-7425-7464</orcidid><orcidid>https://orcid.org/0000-0002-0175-554X</orcidid><orcidid>https://orcid.org/0000-0003-0842-8741</orcidid><orcidid>https://orcid.org/0000000260934521</orcidid><orcidid>https://orcid.org/0000000174257464</orcidid><orcidid>https://orcid.org/0000000308428741</orcidid><orcidid>https://orcid.org/000000020175554X</orcidid><orcidid>https://orcid.org/0000000232459081</orcidid></search><sort><creationdate>20220204</creationdate><title>Exogenous chalcone synthase expression in developing poplar xylem incorporates naringenin into lignins</title><author>Mahon, Elizabeth L ; 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| ispartof | Plant physiology (Bethesda), 2022-02, Vol.188 (2), p.984-996 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Acyltransferases - genetics Acyltransferases - metabolism BASIC BIOLOGICAL SCIENCES Crops, Agricultural - genetics Crops, Agricultural - metabolism Flavanones - genetics Flavanones - metabolism Gene Expression Regulation, Plant Genes, Plant Lignin - biosynthesis Lignin - genetics Malus - genetics Malus - metabolism Plants, Genetically Modified - metabolism Populus - genetics Populus - metabolism Regular Issue Content Xylem - genetics Xylem - metabolism |
| title | Exogenous chalcone synthase expression in developing poplar xylem incorporates naringenin into lignins |
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