Association of TNF -α 308G/A and LEPR Gln223Arg Polymorphisms with the Risk of Type 2 Diabetes Mellitus

The objective of the present study was to identify the association of the -α- 308G/A and leptin receptor ( ) Gln223Arg polymorphisms with the risk of development of type 2 diabetes mellitus (T2DM). A total of 160 volunteers were studied: 108 with T2DM and 52 participants as control, who served as the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the genomic region of -α- 308G/A and Gln223Arg were carried out. The frequency of Gln223Arg genotypes in T2DM and control groups showed significant differences in the distribution of genotypes ( < 0.05). The frequency also of 308G/A genotypes in T2DM and control subjects showed significant differences in the distribution of genotypes ( < 0.05). Our results indicate that there are significant differences in the distribution of genotypes and alleles between the individuals with T2DM and control subjects ( < 0.05).