Optimization and diagnostic evaluation of monoclonal antibody-based blocking ELISA formats for detection of neutralizing antibodies to Hendra virus in mammalian sera

•Two Hendra virus monoclonal antibody based ELISAs for multispecies serology diagnosis have been developed.•The two monoclonal antibodies exhibited differing functional characteristics, which enabled identification of false positive reactions and achievement of higher diagnostic specificity.•The two...

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Veröffentlicht in:	Journal of virological methods 2019-12, Vol.274, p.113731-113731, Article 113731
Hauptverfasser:	Di Rubbo, A., McNabb, L., Klein, R., White, J.R., Colling, A., Dimitrov, D.S., Broder, C.C., Middleton, D., Lunt, R.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical specificity and sensitivity Animals Antibodies, Monoclonal - immunology Antibodies, Neutralizing - blood Antibodies, Viral - blood Antigens, Viral - immunology Blocking ELISA Diagnostic specificity and sensitivity Enzyme-Linked Immunosorbent Assay - methods Hendra virus Hendra Virus - immunology Henipavirus Henipavirus Infections - diagnosis Henipavirus Infections - veterinary Humans Indirect ELISA Monoclonal antibody Nipah virus Sensitivity and Specificity Soluble G antigen
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