Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity
Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples ( = 105) in comparison with v...
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| Veröffentlicht in: | Journal of clinical microbiology 2022-01, Vol.60 (1), p.e0160921-e0160921 |
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| creator | Santos Bravo, Marta Berengua, Carla Marín, Pilar Esteban, Montserrat Rodriguez, Cristina Del Cuerpo, Margarita Miró, Elisenda Cuesta, Genoveva Mosquera, Mar Sánchez-Palomino, Sonsoles Vila, Jordi Rabella, Núria Marcos, María Ángeles |
| description | Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (
= 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97,
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| doi_str_mv | 10.1128/jcm.01609-21 |
| format | Article |
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= 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97,
< 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (
) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.01609-21</identifier><identifier>PMID: 34669457</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Biomarkers ; Clinical Microbiology ; COVID-19 ; Humans ; Reverse Transcription ; RNA ; RNA, Viral - genetics ; SARS-CoV-2 ; Virology</subject><ispartof>Journal of clinical microbiology, 2022-01, Vol.60 (1), p.e0160921-e0160921</ispartof><rights>Copyright © 2022 American Society for Microbiology.</rights><rights>Copyright © 2022 American Society for Microbiology. 2022 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a484t-e72ee35951bd995f91548bdd52a0bb9c6ccacbc11d3a37c7fd7484fc64888d173</citedby><cites>FETCH-LOGICAL-a484t-e72ee35951bd995f91548bdd52a0bb9c6ccacbc11d3a37c7fd7484fc64888d173</cites><orcidid>0000-0002-8025-3926 ; 0000-0001-9963-3809</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.asm.org/doi/pdf/10.1128/JCM.01609-21$$EPDF$$P50$$Gasm2$$H</linktopdf><linktohtml>$$Uhttps://journals.asm.org/doi/full/10.1128/JCM.01609-21$$EHTML$$P50$$Gasm2$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,27929,27930,52756,52757,52758,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34669457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Caliendo, Angela M</contributor><contributor>Caliendo, Angela M.</contributor><creatorcontrib>Santos Bravo, Marta</creatorcontrib><creatorcontrib>Berengua, Carla</creatorcontrib><creatorcontrib>Marín, Pilar</creatorcontrib><creatorcontrib>Esteban, Montserrat</creatorcontrib><creatorcontrib>Rodriguez, Cristina</creatorcontrib><creatorcontrib>Del Cuerpo, Margarita</creatorcontrib><creatorcontrib>Miró, Elisenda</creatorcontrib><creatorcontrib>Cuesta, Genoveva</creatorcontrib><creatorcontrib>Mosquera, Mar</creatorcontrib><creatorcontrib>Sánchez-Palomino, Sonsoles</creatorcontrib><creatorcontrib>Vila, Jordi</creatorcontrib><creatorcontrib>Rabella, Núria</creatorcontrib><creatorcontrib>Marcos, María Ángeles</creatorcontrib><title>Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><addtitle>J Clin Microbiol</addtitle><description>Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (
= 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97,
< 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (
) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions.</description><subject>Biomarkers</subject><subject>Clinical Microbiology</subject><subject>COVID-19</subject><subject>Humans</subject><subject>Reverse Transcription</subject><subject>RNA</subject><subject>RNA, Viral - genetics</subject><subject>SARS-CoV-2</subject><subject>Virology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v1DAURS0EokNhxxp5CRIpfo4_N0ijCEpRC1IHRuwsx3EGD0nc2nGl_nsCUypYsHoLH5-n-y5Cz4GcAFD1Zu_GEwKC6IrCA7QColUlBPn2EK0I0bwCqOURepLznhBgjPPH6KhmQmjG5QrZbUh2wE0Z5pI8buLUhzT6Dm_Wl5uqiduK4k1pd36KY3D48tMab-1QPLYZW3wa40KWlOLOzh5f2PTDJxx7fDb13s3hJsy3T9Gj3g7ZP7ubx-jr-3dfmg_V-efTs2Z9Xlmm2Fx5Sb2vuebQdlrzXgNnqu06Ti1pW-2Ec9a1DqCrbS2d7Du5_OudYEqpDmR9jN4evFelXQI4P81LMnOVwmjTrYk2mH9fpvDd7OKNUVJoCWoRvLwTpHhdfJ7NGLLzw2AnH0s2lCvOAATVC_r6gLoUc06-v18DxPxqxXxsLszvVgyFBX91wG0eqdnHkqblEv9jX_wd4178p7L6JwyFlUY</recordid><startdate>20220119</startdate><enddate>20220119</enddate><creator>Santos Bravo, Marta</creator><creator>Berengua, Carla</creator><creator>Marín, Pilar</creator><creator>Esteban, Montserrat</creator><creator>Rodriguez, Cristina</creator><creator>Del Cuerpo, Margarita</creator><creator>Miró, Elisenda</creator><creator>Cuesta, Genoveva</creator><creator>Mosquera, Mar</creator><creator>Sánchez-Palomino, Sonsoles</creator><creator>Vila, Jordi</creator><creator>Rabella, Núria</creator><creator>Marcos, María Ángeles</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8025-3926</orcidid><orcidid>https://orcid.org/0000-0001-9963-3809</orcidid></search><sort><creationdate>20220119</creationdate><title>Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity</title><author>Santos Bravo, Marta ; Berengua, Carla ; Marín, Pilar ; Esteban, Montserrat ; Rodriguez, Cristina ; Del Cuerpo, Margarita ; Miró, Elisenda ; Cuesta, Genoveva ; Mosquera, Mar ; Sánchez-Palomino, Sonsoles ; Vila, Jordi ; Rabella, Núria ; Marcos, María Ángeles</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a484t-e72ee35951bd995f91548bdd52a0bb9c6ccacbc11d3a37c7fd7484fc64888d173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomarkers</topic><topic>Clinical Microbiology</topic><topic>COVID-19</topic><topic>Humans</topic><topic>Reverse Transcription</topic><topic>RNA</topic><topic>RNA, Viral - genetics</topic><topic>SARS-CoV-2</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos Bravo, Marta</creatorcontrib><creatorcontrib>Berengua, Carla</creatorcontrib><creatorcontrib>Marín, Pilar</creatorcontrib><creatorcontrib>Esteban, Montserrat</creatorcontrib><creatorcontrib>Rodriguez, Cristina</creatorcontrib><creatorcontrib>Del Cuerpo, Margarita</creatorcontrib><creatorcontrib>Miró, Elisenda</creatorcontrib><creatorcontrib>Cuesta, Genoveva</creatorcontrib><creatorcontrib>Mosquera, Mar</creatorcontrib><creatorcontrib>Sánchez-Palomino, Sonsoles</creatorcontrib><creatorcontrib>Vila, Jordi</creatorcontrib><creatorcontrib>Rabella, Núria</creatorcontrib><creatorcontrib>Marcos, María Ángeles</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos Bravo, Marta</au><au>Berengua, Carla</au><au>Marín, Pilar</au><au>Esteban, Montserrat</au><au>Rodriguez, Cristina</au><au>Del Cuerpo, Margarita</au><au>Miró, Elisenda</au><au>Cuesta, Genoveva</au><au>Mosquera, Mar</au><au>Sánchez-Palomino, Sonsoles</au><au>Vila, Jordi</au><au>Rabella, Núria</au><au>Marcos, María Ángeles</au><au>Caliendo, Angela M</au><au>Caliendo, Angela M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity</atitle><jtitle>Journal of clinical microbiology</jtitle><stitle>J Clin Microbiol</stitle><addtitle>J Clin Microbiol</addtitle><date>2022-01-19</date><risdate>2022</risdate><volume>60</volume><issue>1</issue><spage>e0160921</spage><epage>e0160921</epage><pages>e0160921-e0160921</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (
= 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97,
< 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (
) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>34669457</pmid><doi>10.1128/jcm.01609-21</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-8025-3926</orcidid><orcidid>https://orcid.org/0000-0001-9963-3809</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biomarkers Clinical Microbiology COVID-19 Humans Reverse Transcription RNA RNA, Viral - genetics SARS-CoV-2 Virology |
| title | Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity |
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