The expression and clinical significance of CD59 and FLAER in Chinese adult AML patients
Background The role of CD59 and fluorescently labeled aerolysin (FLAER) in acute myeloid leukemia (AML) remains unclear and requires further investigation. To explore the relationship between CD59, FLAER, and AML, we investigated CD59 and FLAER expression in AML and analyzed their relationship with...
Gespeichert in:
| Veröffentlicht in: | Journal of clinical laboratory analysis 2022-01, Vol.36 (1), p.e24145-n/a |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 1 |
| container_start_page | e24145 |
| container_title | Journal of clinical laboratory analysis |
| container_volume | 36 |
| creator | Li, Lijuan Yu, Shunjie Liu, Shanshan Meng, Fanqiao Ren, Xiaotong Liu, Zhaoyun Fu, Rong |
| description | Background
The role of CD59 and fluorescently labeled aerolysin (FLAER) in acute myeloid leukemia (AML) remains unclear and requires further investigation. To explore the relationship between CD59, FLAER, and AML, we investigated CD59 and FLAER expression in AML and analyzed their relationship with clinical characteristics of AML patients.
Methods
We employed flow cytometry (FCM) to analyze CD59 and FLAER expression in 161 AML patients at Tianjin Medical University General Hospital and evaluated its association with sex, white blood cell (WBC) count, platelet (PLT) count, thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D‐Dimer(D‐D), and lactate dehydrogenase (LDH), followed by analyzing its connection with disease progression and complete remission (CR).
Results
CD59 and FLAER deficiencies were identified in AML patients. Compared with CR group, non‐CR group patients revealed more CD59 and FLAER deficiency. Compared with non‐acute promyelocytic leukemia (M3) group, M3 group patients had more CD59 and FLAER deficiency. CD59− level in primordial cells of M3 patients was positively correlated with primordial cell ratio (r = 0.660, p = 0.003). Additionally, we discovered that the decline in CD59 and FLAER levels might be linked to higher D‐D and LDH in AML patients. The difference was statistically significant (p |
| doi_str_mv | 10.1002/jcla.24145 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8761415</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2619993696</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4075-25440165f5a233ddcecb3ba85d8c63e863d9c6ac34d95a10d743f90d1642c5753</originalsourceid><addsrcrecordid>eNp90VtLHDEYBuBQKnW1vekPkEBvRBib8yQ3hWU8M6UgCr0L2STjZslm1smMh3_vrKtSvehVAnny8n28AHzH6BAjRH4ubDSHhGHGP4EJRkoWRBL-GUyQlGUhEabbYCfnBUJIKiy-gG3KFOVY8Qn4ezX30D-sOp9zaBM0yUEbQwrWRJjDTQrNeE3Ww7aB1RFXz-Kknh5fwpBgNQ_JZw-NG2IPp79ruDJ98KnPX8FWY2L2317OXXB9cnxVnRX1n9PzaloXlqGSF4QzhrDgDTeEUuestzM6M5I7aQX1UlCnrDCWMqe4wciVjDYKOSwYsbzkdBf82uSuhtnSj_9T35moV11Ymu5Rtybo9y8pzPVNe6dlKTDD64D9l4CuvR187vUyZOtjNMm3Q9ZEYFJSSpEc6Y8PdNEOXRrXWyulFBVKjOpgo2zX5tz55m0YjPS6ML0uTD8XNuK9f8d_o68NjQBvwH2I_vE_Ufqiqqeb0Cf9wJ7a</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2619993696</pqid></control><display><type>article</type><title>The expression and clinical significance of CD59 and FLAER in Chinese adult AML patients</title><source>Wiley Online Library - AutoHoldings Journals</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Li, Lijuan ; Yu, Shunjie ; Liu, Shanshan ; Meng, Fanqiao ; Ren, Xiaotong ; Liu, Zhaoyun ; Fu, Rong</creator><creatorcontrib>Li, Lijuan ; Yu, Shunjie ; Liu, Shanshan ; Meng, Fanqiao ; Ren, Xiaotong ; Liu, Zhaoyun ; Fu, Rong</creatorcontrib><description>Background
The role of CD59 and fluorescently labeled aerolysin (FLAER) in acute myeloid leukemia (AML) remains unclear and requires further investigation. To explore the relationship between CD59, FLAER, and AML, we investigated CD59 and FLAER expression in AML and analyzed their relationship with clinical characteristics of AML patients.
Methods
We employed flow cytometry (FCM) to analyze CD59 and FLAER expression in 161 AML patients at Tianjin Medical University General Hospital and evaluated its association with sex, white blood cell (WBC) count, platelet (PLT) count, thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D‐Dimer(D‐D), and lactate dehydrogenase (LDH), followed by analyzing its connection with disease progression and complete remission (CR).
Results
CD59 and FLAER deficiencies were identified in AML patients. Compared with CR group, non‐CR group patients revealed more CD59 and FLAER deficiency. Compared with non‐acute promyelocytic leukemia (M3) group, M3 group patients had more CD59 and FLAER deficiency. CD59− level in primordial cells of M3 patients was positively correlated with primordial cell ratio (r = 0.660, p = 0.003). Additionally, we discovered that the decline in CD59 and FLAER levels might be linked to higher D‐D and LDH in AML patients. The difference was statistically significant (p < 0.05).
Conclusions
We demonstrated that the decline in CD59 and FLAER levels was associated with leukemia cell proliferation and abnormal coagulation function in AML, suggesting that they could serve as a predictor of AML coagulation dysfunction, particularly in M3.
The decline in CD59 and FLAER levels was associated with leukemia cell proliferation and abnormal coagulation function in AML, suggesting that they could serve as a predictor of AML coagulation dysfunction, particularly in M3.</description><identifier>ISSN: 0887-8013</identifier><identifier>EISSN: 1098-2825</identifier><identifier>DOI: 10.1002/jcla.24145</identifier><identifier>PMID: 34935195</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Acute myeloid leukemia ; Acute promyeloid leukemia ; CD59 ; CD59 antigen ; Cell proliferation ; Clinical significance ; Coagulation ; coagulation function ; FLAER ; Flow cytometry ; Granulocytes ; L-Lactate dehydrogenase ; Leukemia ; Proteins ; Prothrombin ; Remission ; Statistical analysis ; Thrombin</subject><ispartof>Journal of clinical laboratory analysis, 2022-01, Vol.36 (1), p.e24145-n/a</ispartof><rights>2021 The Authors. published by Wiley Periodicals LLC.</rights><rights>2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4075-25440165f5a233ddcecb3ba85d8c63e863d9c6ac34d95a10d743f90d1642c5753</cites><orcidid>0000-0001-8913-3915</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761415/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761415/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34935195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Lijuan</creatorcontrib><creatorcontrib>Yu, Shunjie</creatorcontrib><creatorcontrib>Liu, Shanshan</creatorcontrib><creatorcontrib>Meng, Fanqiao</creatorcontrib><creatorcontrib>Ren, Xiaotong</creatorcontrib><creatorcontrib>Liu, Zhaoyun</creatorcontrib><creatorcontrib>Fu, Rong</creatorcontrib><title>The expression and clinical significance of CD59 and FLAER in Chinese adult AML patients</title><title>Journal of clinical laboratory analysis</title><addtitle>J Clin Lab Anal</addtitle><description>Background
The role of CD59 and fluorescently labeled aerolysin (FLAER) in acute myeloid leukemia (AML) remains unclear and requires further investigation. To explore the relationship between CD59, FLAER, and AML, we investigated CD59 and FLAER expression in AML and analyzed their relationship with clinical characteristics of AML patients.
Methods
We employed flow cytometry (FCM) to analyze CD59 and FLAER expression in 161 AML patients at Tianjin Medical University General Hospital and evaluated its association with sex, white blood cell (WBC) count, platelet (PLT) count, thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D‐Dimer(D‐D), and lactate dehydrogenase (LDH), followed by analyzing its connection with disease progression and complete remission (CR).
Results
CD59 and FLAER deficiencies were identified in AML patients. Compared with CR group, non‐CR group patients revealed more CD59 and FLAER deficiency. Compared with non‐acute promyelocytic leukemia (M3) group, M3 group patients had more CD59 and FLAER deficiency. CD59− level in primordial cells of M3 patients was positively correlated with primordial cell ratio (r = 0.660, p = 0.003). Additionally, we discovered that the decline in CD59 and FLAER levels might be linked to higher D‐D and LDH in AML patients. The difference was statistically significant (p < 0.05).
Conclusions
We demonstrated that the decline in CD59 and FLAER levels was associated with leukemia cell proliferation and abnormal coagulation function in AML, suggesting that they could serve as a predictor of AML coagulation dysfunction, particularly in M3.
The decline in CD59 and FLAER levels was associated with leukemia cell proliferation and abnormal coagulation function in AML, suggesting that they could serve as a predictor of AML coagulation dysfunction, particularly in M3.</description><subject>Acute myeloid leukemia</subject><subject>Acute promyeloid leukemia</subject><subject>CD59</subject><subject>CD59 antigen</subject><subject>Cell proliferation</subject><subject>Clinical significance</subject><subject>Coagulation</subject><subject>coagulation function</subject><subject>FLAER</subject><subject>Flow cytometry</subject><subject>Granulocytes</subject><subject>L-Lactate dehydrogenase</subject><subject>Leukemia</subject><subject>Proteins</subject><subject>Prothrombin</subject><subject>Remission</subject><subject>Statistical analysis</subject><subject>Thrombin</subject><issn>0887-8013</issn><issn>1098-2825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90VtLHDEYBuBQKnW1vekPkEBvRBib8yQ3hWU8M6UgCr0L2STjZslm1smMh3_vrKtSvehVAnny8n28AHzH6BAjRH4ubDSHhGHGP4EJRkoWRBL-GUyQlGUhEabbYCfnBUJIKiy-gG3KFOVY8Qn4ezX30D-sOp9zaBM0yUEbQwrWRJjDTQrNeE3Ww7aB1RFXz-Kknh5fwpBgNQ_JZw-NG2IPp79ruDJ98KnPX8FWY2L2317OXXB9cnxVnRX1n9PzaloXlqGSF4QzhrDgDTeEUuestzM6M5I7aQX1UlCnrDCWMqe4wciVjDYKOSwYsbzkdBf82uSuhtnSj_9T35moV11Ymu5Rtybo9y8pzPVNe6dlKTDD64D9l4CuvR187vUyZOtjNMm3Q9ZEYFJSSpEc6Y8PdNEOXRrXWyulFBVKjOpgo2zX5tz55m0YjPS6ML0uTD8XNuK9f8d_o68NjQBvwH2I_vE_Ufqiqqeb0Cf9wJ7a</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Li, Lijuan</creator><creator>Yu, Shunjie</creator><creator>Liu, Shanshan</creator><creator>Meng, Fanqiao</creator><creator>Ren, Xiaotong</creator><creator>Liu, Zhaoyun</creator><creator>Fu, Rong</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8913-3915</orcidid></search><sort><creationdate>202201</creationdate><title>The expression and clinical significance of CD59 and FLAER in Chinese adult AML patients</title><author>Li, Lijuan ; Yu, Shunjie ; Liu, Shanshan ; Meng, Fanqiao ; Ren, Xiaotong ; Liu, Zhaoyun ; Fu, Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4075-25440165f5a233ddcecb3ba85d8c63e863d9c6ac34d95a10d743f90d1642c5753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acute myeloid leukemia</topic><topic>Acute promyeloid leukemia</topic><topic>CD59</topic><topic>CD59 antigen</topic><topic>Cell proliferation</topic><topic>Clinical significance</topic><topic>Coagulation</topic><topic>coagulation function</topic><topic>FLAER</topic><topic>Flow cytometry</topic><topic>Granulocytes</topic><topic>L-Lactate dehydrogenase</topic><topic>Leukemia</topic><topic>Proteins</topic><topic>Prothrombin</topic><topic>Remission</topic><topic>Statistical analysis</topic><topic>Thrombin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Lijuan</creatorcontrib><creatorcontrib>Yu, Shunjie</creatorcontrib><creatorcontrib>Liu, Shanshan</creatorcontrib><creatorcontrib>Meng, Fanqiao</creatorcontrib><creatorcontrib>Ren, Xiaotong</creatorcontrib><creatorcontrib>Liu, Zhaoyun</creatorcontrib><creatorcontrib>Fu, Rong</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical laboratory analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Lijuan</au><au>Yu, Shunjie</au><au>Liu, Shanshan</au><au>Meng, Fanqiao</au><au>Ren, Xiaotong</au><au>Liu, Zhaoyun</au><au>Fu, Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The expression and clinical significance of CD59 and FLAER in Chinese adult AML patients</atitle><jtitle>Journal of clinical laboratory analysis</jtitle><addtitle>J Clin Lab Anal</addtitle><date>2022-01</date><risdate>2022</risdate><volume>36</volume><issue>1</issue><spage>e24145</spage><epage>n/a</epage><pages>e24145-n/a</pages><issn>0887-8013</issn><eissn>1098-2825</eissn><abstract>Background
The role of CD59 and fluorescently labeled aerolysin (FLAER) in acute myeloid leukemia (AML) remains unclear and requires further investigation. To explore the relationship between CD59, FLAER, and AML, we investigated CD59 and FLAER expression in AML and analyzed their relationship with clinical characteristics of AML patients.
Methods
We employed flow cytometry (FCM) to analyze CD59 and FLAER expression in 161 AML patients at Tianjin Medical University General Hospital and evaluated its association with sex, white blood cell (WBC) count, platelet (PLT) count, thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D‐Dimer(D‐D), and lactate dehydrogenase (LDH), followed by analyzing its connection with disease progression and complete remission (CR).
Results
CD59 and FLAER deficiencies were identified in AML patients. Compared with CR group, non‐CR group patients revealed more CD59 and FLAER deficiency. Compared with non‐acute promyelocytic leukemia (M3) group, M3 group patients had more CD59 and FLAER deficiency. CD59− level in primordial cells of M3 patients was positively correlated with primordial cell ratio (r = 0.660, p = 0.003). Additionally, we discovered that the decline in CD59 and FLAER levels might be linked to higher D‐D and LDH in AML patients. The difference was statistically significant (p < 0.05).
Conclusions
We demonstrated that the decline in CD59 and FLAER levels was associated with leukemia cell proliferation and abnormal coagulation function in AML, suggesting that they could serve as a predictor of AML coagulation dysfunction, particularly in M3.
The decline in CD59 and FLAER levels was associated with leukemia cell proliferation and abnormal coagulation function in AML, suggesting that they could serve as a predictor of AML coagulation dysfunction, particularly in M3.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>34935195</pmid><doi>10.1002/jcla.24145</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0001-8913-3915</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-8013 |
| ispartof | Journal of clinical laboratory analysis, 2022-01, Vol.36 (1), p.e24145-n/a |
| issn | 0887-8013 1098-2825 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8761415 |
| source | Wiley Online Library - AutoHoldings Journals; DOAJ Directory of Open Access Journals; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acute myeloid leukemia Acute promyeloid leukemia CD59 CD59 antigen Cell proliferation Clinical significance Coagulation coagulation function FLAER Flow cytometry Granulocytes L-Lactate dehydrogenase Leukemia Proteins Prothrombin Remission Statistical analysis Thrombin |
| title | The expression and clinical significance of CD59 and FLAER in Chinese adult AML patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T12%3A13%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20expression%20and%20clinical%20significance%20of%20CD59%20and%20FLAER%20in%20Chinese%20adult%20AML%20patients&rft.jtitle=Journal%20of%20clinical%20laboratory%20analysis&rft.au=Li,%20Lijuan&rft.date=2022-01&rft.volume=36&rft.issue=1&rft.spage=e24145&rft.epage=n/a&rft.pages=e24145-n/a&rft.issn=0887-8013&rft.eissn=1098-2825&rft_id=info:doi/10.1002/jcla.24145&rft_dat=%3Cproquest_pubme%3E2619993696%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2619993696&rft_id=info:pmid/34935195&rfr_iscdi=true |