Chemical capping improves template switching and enhances sequencing of small RNAs

Abstract Template-switching reverse transcription is widely used in RNA sequencing for low-input and low-quality samples, including RNA from single cells or formalin-fixed paraffin-embedded (FFPE) tissues. Previously, we identified the native eukaryotic mRNA 5′ cap as a key structural element for en...

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Veröffentlicht in:	Nucleic acids research 2022-01, Vol.50 (1), p.e2-e2
Hauptverfasser:	Wulf, Madalee G, Maguire, Sean, Dai, Nan, Blondel, Alice, Posfai, Dora, Krishnan, Keerthana, Sun, Zhiyi, Guan, Shengxi, Corrêa, Ivan R
Format:	Artikel
Sprache:	eng
Schlagworte:	Humans Methods Online RNA - analysis RNA Caps - metabolism Sequence Analysis, RNA - methods Tissue Fixation
Online-Zugang:	Volltext
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