CNV Hotspots in Testicular Seminoma Tissue and Seminal Plasma
Seminoma (SE) is the most frequent type of testicular tumour, affecting predominantly young men. Early detection and diagnosis of SE could significantly improve life quality and reproductive health after diagnosis and treatment. Copy number variation (CNV) has already been associated with various ca...
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| Veröffentlicht in: | Cancers 2021-12, Vol.14 (1), p.189 |
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| creator | Raos, Dora Abramović, Irena Tomić, Miroslav Vrtarić, Alen Kuliš, Tomislav Ćorić, Marijana Ulamec, Monika Katušić Bojanac, Ana Ježek, Davor Sinčić, Nino |
| description | Seminoma (SE) is the most frequent type of testicular tumour, affecting predominantly young men. Early detection and diagnosis of SE could significantly improve life quality and reproductive health after diagnosis and treatment. Copy number variation (CNV) has already been associated with various cancers as well as SE. In this study, we selected four genes (
,
,
, and
) for CNV analysis in genomic DNA (gDNA), which are located on chromosomes susceptible to gains, and whose aberrant expression was already detected in SE. Furthermore, CNV was analysed in cell-free DNA (cfDNA) from seminal plasma. Analysis was performed by droplet digital polymerase chain reaction (ddPCR) on gDNA from SE and nonmalignant testicular tissue. Seminal plasma cfDNA from SE patients before and after surgery was analysed, as well as from healthy volunteers. The CNV hotspot in gDNA from SE tissue was detected for the first time in all analysed genes, and for two genes,
and
it was reflected in cfDNA from seminal plasma. Although clinical value is yet to be determined, presented data emphasize a potential use of CNV as a potential SE biomarker from a liquid biopsy. |
| doi_str_mv | 10.3390/cancers14010189 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8750740</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2618909211</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-74ff27370c1f5288cddc00bd08788770da37bd4b9c94df5fa5fc8503d3be9da23</originalsourceid><addsrcrecordid>eNpdkd1LwzAUxYMobsw9-yYFX3yp3iTNkj4oyFAnDBWcvoY0SbWjHzNpBf97MzrHXOCScPPL4Z4chE4xXFKawpVWtbbO4wQwYJEeoCEBTuLJJE0Od84DNPZ-CWFRivmEH6MBZQCCMjJE19On92jWtH4VKirqaGF9W-iuVC56tVVRN5WKFoX3nY1UbfqeKqOXUvlKnaCjXJXejjf7CL3d3y2ms3j-_PA4vZ3HOiG4jXmS54RTDhrnjAihjdEAmQHBheAcjKI8M0mW6jQxOcsVy7VgQA3NbGoUoSN00-uuuqyyRtu6daqUK1dUyv3IRhXy_01dfMqP5lsKzoAnEAQuNgKu-eqCRVkVXtuyVLVtOi_JJHwgpATjgJ7vocumc8FzTxFgjK0nuuop7Rrvnc23w2CQ63TkXjrhxdmuhy3_lwX9BfeXi9E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2618205552</pqid></control><display><type>article</type><title>CNV Hotspots in Testicular Seminoma Tissue and Seminal Plasma</title><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Raos, Dora ; Abramović, Irena ; Tomić, Miroslav ; Vrtarić, Alen ; Kuliš, Tomislav ; Ćorić, Marijana ; Ulamec, Monika ; Katušić Bojanac, Ana ; Ježek, Davor ; Sinčić, Nino</creator><creatorcontrib>Raos, Dora ; Abramović, Irena ; Tomić, Miroslav ; Vrtarić, Alen ; Kuliš, Tomislav ; Ćorić, Marijana ; Ulamec, Monika ; Katušić Bojanac, Ana ; Ježek, Davor ; Sinčić, Nino</creatorcontrib><description>Seminoma (SE) is the most frequent type of testicular tumour, affecting predominantly young men. Early detection and diagnosis of SE could significantly improve life quality and reproductive health after diagnosis and treatment. Copy number variation (CNV) has already been associated with various cancers as well as SE. In this study, we selected four genes (
,
,
, and
) for CNV analysis in genomic DNA (gDNA), which are located on chromosomes susceptible to gains, and whose aberrant expression was already detected in SE. Furthermore, CNV was analysed in cell-free DNA (cfDNA) from seminal plasma. Analysis was performed by droplet digital polymerase chain reaction (ddPCR) on gDNA from SE and nonmalignant testicular tissue. Seminal plasma cfDNA from SE patients before and after surgery was analysed, as well as from healthy volunteers. The CNV hotspot in gDNA from SE tissue was detected for the first time in all analysed genes, and for two genes,
and
it was reflected in cfDNA from seminal plasma. Although clinical value is yet to be determined, presented data emphasize a potential use of CNV as a potential SE biomarker from a liquid biopsy.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers14010189</identifier><identifier>PMID: 35008352</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Archives & records ; Biomarkers ; Biopsy ; Cancer ; Chromosomes ; Copy number ; Deoxyribonucleic acid ; Diagnosis ; DNA ; DNA methylation ; Genes ; Genetic engineering ; Genomes ; Genomic analysis ; Patients ; Plasma ; Polymerase chain reaction ; Quality of life ; Reproductive health ; Semen ; Seminoma ; Surgery ; Testes ; Tumors</subject><ispartof>Cancers, 2021-12, Vol.14 (1), p.189</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-74ff27370c1f5288cddc00bd08788770da37bd4b9c94df5fa5fc8503d3be9da23</citedby><cites>FETCH-LOGICAL-c421t-74ff27370c1f5288cddc00bd08788770da37bd4b9c94df5fa5fc8503d3be9da23</cites><orcidid>0000-0002-0895-5691 ; 0000-0001-7507-0394 ; 0000-0002-1528-5462</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750740/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750740/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35008352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raos, Dora</creatorcontrib><creatorcontrib>Abramović, Irena</creatorcontrib><creatorcontrib>Tomić, Miroslav</creatorcontrib><creatorcontrib>Vrtarić, Alen</creatorcontrib><creatorcontrib>Kuliš, Tomislav</creatorcontrib><creatorcontrib>Ćorić, Marijana</creatorcontrib><creatorcontrib>Ulamec, Monika</creatorcontrib><creatorcontrib>Katušić Bojanac, Ana</creatorcontrib><creatorcontrib>Ježek, Davor</creatorcontrib><creatorcontrib>Sinčić, Nino</creatorcontrib><title>CNV Hotspots in Testicular Seminoma Tissue and Seminal Plasma</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>Seminoma (SE) is the most frequent type of testicular tumour, affecting predominantly young men. Early detection and diagnosis of SE could significantly improve life quality and reproductive health after diagnosis and treatment. Copy number variation (CNV) has already been associated with various cancers as well as SE. In this study, we selected four genes (
,
,
, and
) for CNV analysis in genomic DNA (gDNA), which are located on chromosomes susceptible to gains, and whose aberrant expression was already detected in SE. Furthermore, CNV was analysed in cell-free DNA (cfDNA) from seminal plasma. Analysis was performed by droplet digital polymerase chain reaction (ddPCR) on gDNA from SE and nonmalignant testicular tissue. Seminal plasma cfDNA from SE patients before and after surgery was analysed, as well as from healthy volunteers. The CNV hotspot in gDNA from SE tissue was detected for the first time in all analysed genes, and for two genes,
and
it was reflected in cfDNA from seminal plasma. Although clinical value is yet to be determined, presented data emphasize a potential use of CNV as a potential SE biomarker from a liquid biopsy.</description><subject>Archives & records</subject><subject>Biomarkers</subject><subject>Biopsy</subject><subject>Cancer</subject><subject>Chromosomes</subject><subject>Copy number</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Genomic analysis</subject><subject>Patients</subject><subject>Plasma</subject><subject>Polymerase chain reaction</subject><subject>Quality of life</subject><subject>Reproductive health</subject><subject>Semen</subject><subject>Seminoma</subject><subject>Surgery</subject><subject>Testes</subject><subject>Tumors</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkd1LwzAUxYMobsw9-yYFX3yp3iTNkj4oyFAnDBWcvoY0SbWjHzNpBf97MzrHXOCScPPL4Z4chE4xXFKawpVWtbbO4wQwYJEeoCEBTuLJJE0Od84DNPZ-CWFRivmEH6MBZQCCMjJE19On92jWtH4VKirqaGF9W-iuVC56tVVRN5WKFoX3nY1UbfqeKqOXUvlKnaCjXJXejjf7CL3d3y2ms3j-_PA4vZ3HOiG4jXmS54RTDhrnjAihjdEAmQHBheAcjKI8M0mW6jQxOcsVy7VgQA3NbGoUoSN00-uuuqyyRtu6daqUK1dUyv3IRhXy_01dfMqP5lsKzoAnEAQuNgKu-eqCRVkVXtuyVLVtOi_JJHwgpATjgJ7vocumc8FzTxFgjK0nuuop7Rrvnc23w2CQ63TkXjrhxdmuhy3_lwX9BfeXi9E</recordid><startdate>20211231</startdate><enddate>20211231</enddate><creator>Raos, Dora</creator><creator>Abramović, Irena</creator><creator>Tomić, Miroslav</creator><creator>Vrtarić, Alen</creator><creator>Kuliš, Tomislav</creator><creator>Ćorić, Marijana</creator><creator>Ulamec, Monika</creator><creator>Katušić Bojanac, Ana</creator><creator>Ježek, Davor</creator><creator>Sinčić, Nino</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0895-5691</orcidid><orcidid>https://orcid.org/0000-0001-7507-0394</orcidid><orcidid>https://orcid.org/0000-0002-1528-5462</orcidid></search><sort><creationdate>20211231</creationdate><title>CNV Hotspots in Testicular Seminoma Tissue and Seminal Plasma</title><author>Raos, Dora ; Abramović, Irena ; Tomić, Miroslav ; Vrtarić, Alen ; Kuliš, Tomislav ; Ćorić, Marijana ; Ulamec, Monika ; Katušić Bojanac, Ana ; Ježek, Davor ; Sinčić, Nino</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-74ff27370c1f5288cddc00bd08788770da37bd4b9c94df5fa5fc8503d3be9da23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Archives & records</topic><topic>Biomarkers</topic><topic>Biopsy</topic><topic>Cancer</topic><topic>Chromosomes</topic><topic>Copy number</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>DNA</topic><topic>DNA methylation</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Genomic analysis</topic><topic>Patients</topic><topic>Plasma</topic><topic>Polymerase chain reaction</topic><topic>Quality of life</topic><topic>Reproductive health</topic><topic>Semen</topic><topic>Seminoma</topic><topic>Surgery</topic><topic>Testes</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raos, Dora</creatorcontrib><creatorcontrib>Abramović, Irena</creatorcontrib><creatorcontrib>Tomić, Miroslav</creatorcontrib><creatorcontrib>Vrtarić, Alen</creatorcontrib><creatorcontrib>Kuliš, Tomislav</creatorcontrib><creatorcontrib>Ćorić, Marijana</creatorcontrib><creatorcontrib>Ulamec, Monika</creatorcontrib><creatorcontrib>Katušić Bojanac, Ana</creatorcontrib><creatorcontrib>Ježek, Davor</creatorcontrib><creatorcontrib>Sinčić, Nino</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raos, Dora</au><au>Abramović, Irena</au><au>Tomić, Miroslav</au><au>Vrtarić, Alen</au><au>Kuliš, Tomislav</au><au>Ćorić, Marijana</au><au>Ulamec, Monika</au><au>Katušić Bojanac, Ana</au><au>Ježek, Davor</au><au>Sinčić, Nino</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CNV Hotspots in Testicular Seminoma Tissue and Seminal Plasma</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2021-12-31</date><risdate>2021</risdate><volume>14</volume><issue>1</issue><spage>189</spage><pages>189-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>Seminoma (SE) is the most frequent type of testicular tumour, affecting predominantly young men. Early detection and diagnosis of SE could significantly improve life quality and reproductive health after diagnosis and treatment. Copy number variation (CNV) has already been associated with various cancers as well as SE. In this study, we selected four genes (
,
,
, and
) for CNV analysis in genomic DNA (gDNA), which are located on chromosomes susceptible to gains, and whose aberrant expression was already detected in SE. Furthermore, CNV was analysed in cell-free DNA (cfDNA) from seminal plasma. Analysis was performed by droplet digital polymerase chain reaction (ddPCR) on gDNA from SE and nonmalignant testicular tissue. Seminal plasma cfDNA from SE patients before and after surgery was analysed, as well as from healthy volunteers. The CNV hotspot in gDNA from SE tissue was detected for the first time in all analysed genes, and for two genes,
and
it was reflected in cfDNA from seminal plasma. Although clinical value is yet to be determined, presented data emphasize a potential use of CNV as a potential SE biomarker from a liquid biopsy.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35008352</pmid><doi>10.3390/cancers14010189</doi><orcidid>https://orcid.org/0000-0002-0895-5691</orcidid><orcidid>https://orcid.org/0000-0001-7507-0394</orcidid><orcidid>https://orcid.org/0000-0002-1528-5462</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Archives & records Biomarkers Biopsy Cancer Chromosomes Copy number Deoxyribonucleic acid Diagnosis DNA DNA methylation Genes Genetic engineering Genomes Genomic analysis Patients Plasma Polymerase chain reaction Quality of life Reproductive health Semen Seminoma Surgery Testes Tumors |
| title | CNV Hotspots in Testicular Seminoma Tissue and Seminal Plasma |
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