Simultaneous ribosome profiling of hundreds of microbes from the human microbiome

Ribosome profiling enables sequencing of ribosome-bound fragments of RNA, revealing which transcripts are being translated as well as the position of ribosomes along mRNAs. Although ribosome profiling has been applied to cultured bacterial isolates, its application to uncultured, mixed communities h...

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Veröffentlicht in:Nature protocols 2021-10, Vol.16 (10), p.4676-4691
Hauptverfasser: Fremin, Brayon J., Nicolaou, Cosmos, Bhatt, Ami S.
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Sprache:eng
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