Evaluation of a PCR primer based on the isocitrate dehydrogenase gene for detection of Helicobacter pylori in feces

In order to improve detection and identification of Helicobacter pylori in highly contaminated samples, we evaluated new specific primers based on the DNA base sequence within the isocitrate dehydrogenase (icd) gene to amplify a 1,200-bp DNA segment. The specificity of the icd primer was tested agai...

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Veröffentlicht in:	Journal of clinical microbiology 2000-10, Vol.38 (10), p.3755-3758
Hauptverfasser:	ARGYROS, Frauke C, GHOSH, Mayurika, LILI HUANG, MASUBUCHI, Naoko, CAVE, David R, GRÜBEL, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacteriology Biological and medical sciences DNA Primers Duodenal Ulcer - microbiology Feces - microbiology Gram-Negative Bacteria - genetics Gram-Positive Bacteria - genetics Helicobacter Infections - diagnosis Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Houseflies - genetics Houseflies - microbiology Human bacterial diseases Humans icd gene Infectious diseases isocitrate dehydrogenase Isocitrate Dehydrogenase - genetics Medical sciences Polymerase Chain Reaction - methods Sensitivity and Specificity
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