Microvascular Experimentation in the Chick Chorioallantoic Membrane as a Model for Screening Angiogenic Agents including from Gene-Modified Cells
The chick chorioallantoic membrane (CAM) assay model of angiogenesis has been highlighted as a relatively quick, low cost and effective model for the study of pro-angiogenic and anti-angiogenic factors. The chick CAM is a highly vascularised extraembryonic membrane which functions for gas exchange,...
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| Veröffentlicht in: | International journal of molecular sciences 2021-12, Vol.23 (1), p.452 |
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| creator | Kennedy, Donna C Coen, Barbara Wheatley, Antony M McCullagh, Karl J A |
| description | The chick chorioallantoic membrane (CAM) assay model of angiogenesis has been highlighted as a relatively quick, low cost and effective model for the study of pro-angiogenic and anti-angiogenic factors. The chick CAM is a highly vascularised extraembryonic membrane which functions for gas exchange, nutrient exchange and waste removal for the growing chick embryo. It is beneficial as it can function as a treatment screening tool, which bridges the gap between cell based in vitro studies and in vivo animal experimentation. In this review, we explore the benefits and drawbacks of the CAM assay to study microcirculation, by the investigation of each distinct stage of the CAM assay procedure, including cultivation techniques, treatment applications and methods of determining an angiogenic response using this assay. We detail the angiogenic effect of treatments, including drugs, metabolites, genes and cells used in conjunction with the CAM assay, while also highlighting the testing of genetically modified cells. We also present a detailed exploration of the advantages and limitations of different CAM analysis techniques, including visual assessment, histological and molecular analysis along with vascular casting methods and live blood flow observations. |
| doi_str_mv | 10.3390/ijms23010452 |
| format | Article |
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The chick CAM is a highly vascularised extraembryonic membrane which functions for gas exchange, nutrient exchange and waste removal for the growing chick embryo. It is beneficial as it can function as a treatment screening tool, which bridges the gap between cell based in vitro studies and in vivo animal experimentation. In this review, we explore the benefits and drawbacks of the CAM assay to study microcirculation, by the investigation of each distinct stage of the CAM assay procedure, including cultivation techniques, treatment applications and methods of determining an angiogenic response using this assay. We detail the angiogenic effect of treatments, including drugs, metabolites, genes and cells used in conjunction with the CAM assay, while also highlighting the testing of genetically modified cells. We also present a detailed exploration of the advantages and limitations of different CAM analysis techniques, including visual assessment, histological and molecular analysis along with vascular casting methods and live blood flow observations.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms23010452</identifier><identifier>PMID: 35008876</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Angiogenesis ; Angiogenesis Inducing Agents - pharmacology ; Animal research ; Animals ; Antiangiogenics ; Assaying ; Blood flow ; Blood vessels ; Cell culture ; Chick Embryo ; Chorioallantoic membrane ; Chorioallantoic Membrane - metabolism ; Cultivation techniques ; Eggs ; Embryos ; Experimentation ; Gas exchange ; Genetic modification ; Humidity ; In vivo methods and tests ; Membranes ; Metabolites ; Microvasculature ; Neovascularization, Pathologic ; Neovascularization, Physiologic ; Physiology ; Review ; Vascular endothelial growth factor</subject><ispartof>International journal of molecular sciences, 2021-12, Vol.23 (1), p.452</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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We also present a detailed exploration of the advantages and limitations of different CAM analysis techniques, including visual assessment, histological and molecular analysis along with vascular casting methods and live blood flow observations.</description><subject>Angiogenesis</subject><subject>Angiogenesis Inducing Agents - pharmacology</subject><subject>Animal research</subject><subject>Animals</subject><subject>Antiangiogenics</subject><subject>Assaying</subject><subject>Blood flow</subject><subject>Blood vessels</subject><subject>Cell culture</subject><subject>Chick Embryo</subject><subject>Chorioallantoic membrane</subject><subject>Chorioallantoic Membrane - metabolism</subject><subject>Cultivation techniques</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Experimentation</subject><subject>Gas exchange</subject><subject>Genetic modification</subject><subject>Humidity</subject><subject>In vivo methods and tests</subject><subject>Membranes</subject><subject>Metabolites</subject><subject>Microvasculature</subject><subject>Neovascularization, Pathologic</subject><subject>Neovascularization, Physiologic</subject><subject>Physiology</subject><subject>Review</subject><subject>Vascular endothelial growth factor</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU9v1DAQxS0EoqVw44wsceFAYPwnTnJBWq1KQeqKA3C2HGey68WxFzup4GPwjfHSUi1cPCPNz0_z5hHynMEbITp46_ZT5gIYyJo_IOdMcl4BqObhSX9GnuS8B-CC191jciZqgLZt1Dn5tXE2xRuT7eJNopc_DpjchGE2s4uBukDnHdL1ztlv5Y3JReO9CXN0lm5w6pMJSE2mhm7igJ6OMdHPNiEGF7Z0FbYubktv6aqUORdB65fhOBtTnOgVBqzKTzc6HOgavc9PyaPR-IzP7uoF-fr-8sv6Q3X96erjenVdWcn4XImaKWtRDcBko8xYNxylUEpYsKrnYAY5Gtv23PY976RRSvaDQmYVdD20nbgg7251D0s_4WDLesl4fSj2Tfqpo3H630lwO72NN7ptZF0zKAKv7gRS_L5gnvXkssXjeTAuWXPF2g4Ub0RBX_6H7uOSQrH3h-ISJGeFen1LlURyTjjeL8NAH7PWp1kX_MWpgXv4b7jiN2G_p8w</recordid><startdate>20211231</startdate><enddate>20211231</enddate><creator>Kennedy, Donna C</creator><creator>Coen, Barbara</creator><creator>Wheatley, Antony M</creator><creator>McCullagh, Karl J A</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9027-7876</orcidid><orcidid>https://orcid.org/0000-0002-1068-929X</orcidid><orcidid>https://orcid.org/0000-0002-0756-2845</orcidid></search><sort><creationdate>20211231</creationdate><title>Microvascular Experimentation in the Chick Chorioallantoic Membrane as a Model for Screening Angiogenic Agents including from Gene-Modified Cells</title><author>Kennedy, Donna C ; Coen, Barbara ; Wheatley, Antony M ; McCullagh, Karl J A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-3516cce6d01476af572e43663c0c6b20ad4fac8b2cbb294a664bd6e1c609b0893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Angiogenesis</topic><topic>Angiogenesis Inducing Agents - pharmacology</topic><topic>Animal research</topic><topic>Animals</topic><topic>Antiangiogenics</topic><topic>Assaying</topic><topic>Blood flow</topic><topic>Blood vessels</topic><topic>Cell culture</topic><topic>Chick Embryo</topic><topic>Chorioallantoic membrane</topic><topic>Chorioallantoic Membrane - metabolism</topic><topic>Cultivation techniques</topic><topic>Eggs</topic><topic>Embryos</topic><topic>Experimentation</topic><topic>Gas exchange</topic><topic>Genetic modification</topic><topic>Humidity</topic><topic>In vivo methods and tests</topic><topic>Membranes</topic><topic>Metabolites</topic><topic>Microvasculature</topic><topic>Neovascularization, Pathologic</topic><topic>Neovascularization, Physiologic</topic><topic>Physiology</topic><topic>Review</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kennedy, Donna C</creatorcontrib><creatorcontrib>Coen, Barbara</creatorcontrib><creatorcontrib>Wheatley, Antony M</creatorcontrib><creatorcontrib>McCullagh, Karl J A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kennedy, Donna C</au><au>Coen, Barbara</au><au>Wheatley, Antony M</au><au>McCullagh, Karl J A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microvascular Experimentation in the Chick Chorioallantoic Membrane as a Model for Screening Angiogenic Agents including from Gene-Modified Cells</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2021-12-31</date><risdate>2021</risdate><volume>23</volume><issue>1</issue><spage>452</spage><pages>452-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The chick chorioallantoic membrane (CAM) assay model of angiogenesis has been highlighted as a relatively quick, low cost and effective model for the study of pro-angiogenic and anti-angiogenic factors. 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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Angiogenesis Angiogenesis Inducing Agents - pharmacology Animal research Animals Antiangiogenics Assaying Blood flow Blood vessels Cell culture Chick Embryo Chorioallantoic membrane Chorioallantoic Membrane - metabolism Cultivation techniques Eggs Embryos Experimentation Gas exchange Genetic modification Humidity In vivo methods and tests Membranes Metabolites Microvasculature Neovascularization, Pathologic Neovascularization, Physiologic Physiology Review Vascular endothelial growth factor |
| title | Microvascular Experimentation in the Chick Chorioallantoic Membrane as a Model for Screening Angiogenic Agents including from Gene-Modified Cells |
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