Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein

Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein

Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bio-protocol 2021-12, Vol.11 (24), p.e4279-e4279
Hauptverfasser: Dornas, Waleska, Schuppan, Detlef
Format: Artikel
Sprache:eng
Schlagworte:
Biology
Methods
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page e4279
container_issue 24
container_start_page e4279
container_title Bio-protocol
container_volume 11
creator Dornas, Waleska
Schuppan, Detlef
description Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting in apoptosis and genetic mutations. Here, we focus on the 2',7'-dichlorodihydrofluorescein diacetate (DCFH -DA) assay to detect RONS. Although this fluorescence-based assay is widely utilized due to its high sensitivity to detect changes in cellular redox status that allow measuring alterations in RONS over time, its validity has been a matter of controversy. If correctly carried out, its limitations are understood and results are correctly interpreted, the DCFH -DA assay is a valuable tool for cell-based studies.
doi_str_mv 10.21769/BioProtoc.4279
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8720519</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2623880269</sourcerecordid><originalsourceid>FETCH-LOGICAL-c306t-173df3717e4d073de9940ac11ed324c4b1a6db752fdb6d087a39cf7fd792a9303</originalsourceid><addsrcrecordid>eNpVUctOwzAQtBAIUOHMDfkGBwJ-pHF8QYLylApFPM6WY29aozQudlLRvyelUMFpZ7WzszsahA4oOWVUZPLs0vmn4BtvTlMm5AbaZZzTJOesv_kH76D9GN8JIZRnjGbpNtrhfZILyekuah5AxzbAFOoG-xI_gzaNmwMefS7GUGNdW_zomuCXzcsMjIOIXY2Hbu7qMR5AVUX8Fpe4mQDu3ikAs6MTcZRcOTOpfPDWTRY2-LJqfYBowNV7aKvUVYT9n9pDbzfXr4O7ZDi6vR9cDBPDSdYkVHBbckEFpJZ0GKRMiTaUguUsNWlBdWYL0WelLTLbOdJcmlKUVkimJSe8h85XurO2mII1ncegKzULbqrDQnnt1P9J7SZq7OcqF4z0qewEjn8Egv9oITZq6joHVaVr8G1ULGM8zwnLltSzFdUEH2OAcn2GEvUdl1rHpZZxdRuHf79b83_D4V_JNJS8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2623880269</pqid></control><display><type>article</type><title>Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Dornas, Waleska ; Schuppan, Detlef</creator><creatorcontrib>Dornas, Waleska ; Schuppan, Detlef</creatorcontrib><description>Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting in apoptosis and genetic mutations. Here, we focus on the 2',7'-dichlorodihydrofluorescein diacetate (DCFH -DA) assay to detect RONS. Although this fluorescence-based assay is widely utilized due to its high sensitivity to detect changes in cellular redox status that allow measuring alterations in RONS over time, its validity has been a matter of controversy. If correctly carried out, its limitations are understood and results are correctly interpreted, the DCFH -DA assay is a valuable tool for cell-based studies.</description><identifier>ISSN: 2331-8325</identifier><identifier>EISSN: 2331-8325</identifier><identifier>DOI: 10.21769/BioProtoc.4279</identifier><identifier>PMID: 35087931</identifier><language>eng</language><publisher>United States: Bio-Protocol</publisher><subject>Biology ; Methods</subject><ispartof>Bio-protocol, 2021-12, Vol.11 (24), p.e4279-e4279</ispartof><rights>Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.</rights><rights>Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-173df3717e4d073de9940ac11ed324c4b1a6db752fdb6d087a39cf7fd792a9303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720519/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720519/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35087931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dornas, Waleska</creatorcontrib><creatorcontrib>Schuppan, Detlef</creatorcontrib><title>Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein</title><title>Bio-protocol</title><addtitle>Bio Protoc</addtitle><description>Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting in apoptosis and genetic mutations. Here, we focus on the 2',7'-dichlorodihydrofluorescein diacetate (DCFH -DA) assay to detect RONS. Although this fluorescence-based assay is widely utilized due to its high sensitivity to detect changes in cellular redox status that allow measuring alterations in RONS over time, its validity has been a matter of controversy. If correctly carried out, its limitations are understood and results are correctly interpreted, the DCFH -DA assay is a valuable tool for cell-based studies.</description><subject>Biology</subject><subject>Methods</subject><issn>2331-8325</issn><issn>2331-8325</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpVUctOwzAQtBAIUOHMDfkGBwJ-pHF8QYLylApFPM6WY29aozQudlLRvyelUMFpZ7WzszsahA4oOWVUZPLs0vmn4BtvTlMm5AbaZZzTJOesv_kH76D9GN8JIZRnjGbpNtrhfZILyekuah5AxzbAFOoG-xI_gzaNmwMefS7GUGNdW_zomuCXzcsMjIOIXY2Hbu7qMR5AVUX8Fpe4mQDu3ikAs6MTcZRcOTOpfPDWTRY2-LJqfYBowNV7aKvUVYT9n9pDbzfXr4O7ZDi6vR9cDBPDSdYkVHBbckEFpJZ0GKRMiTaUguUsNWlBdWYL0WelLTLbOdJcmlKUVkimJSe8h85XurO2mII1ncegKzULbqrDQnnt1P9J7SZq7OcqF4z0qewEjn8Egv9oITZq6joHVaVr8G1ULGM8zwnLltSzFdUEH2OAcn2GEvUdl1rHpZZxdRuHf79b83_D4V_JNJS8</recordid><startdate>20211220</startdate><enddate>20211220</enddate><creator>Dornas, Waleska</creator><creator>Schuppan, Detlef</creator><general>Bio-Protocol</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20211220</creationdate><title>Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein</title><author>Dornas, Waleska ; Schuppan, Detlef</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-173df3717e4d073de9940ac11ed324c4b1a6db752fdb6d087a39cf7fd792a9303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biology</topic><topic>Methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dornas, Waleska</creatorcontrib><creatorcontrib>Schuppan, Detlef</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bio-protocol</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dornas, Waleska</au><au>Schuppan, Detlef</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein</atitle><jtitle>Bio-protocol</jtitle><addtitle>Bio Protoc</addtitle><date>2021-12-20</date><risdate>2021</risdate><volume>11</volume><issue>24</issue><spage>e4279</spage><epage>e4279</epage><pages>e4279-e4279</pages><issn>2331-8325</issn><eissn>2331-8325</eissn><abstract>Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting in apoptosis and genetic mutations. Here, we focus on the 2',7'-dichlorodihydrofluorescein diacetate (DCFH -DA) assay to detect RONS. Although this fluorescence-based assay is widely utilized due to its high sensitivity to detect changes in cellular redox status that allow measuring alterations in RONS over time, its validity has been a matter of controversy. If correctly carried out, its limitations are understood and results are correctly interpreted, the DCFH -DA assay is a valuable tool for cell-based studies.</abstract><cop>United States</cop><pub>Bio-Protocol</pub><pmid>35087931</pmid><doi>10.21769/BioProtoc.4279</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2331-8325
ispartof Bio-protocol, 2021-12, Vol.11 (24), p.e4279-e4279
issn 2331-8325
2331-8325
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8720519
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Biology
Methods
title Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T10%3A50%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Measurement%20of%20Reactive%20Oxygen%20and%20Nitrogen%20Species%20in%20Living%20Cells%20Using%20the%20Probe%202',7'-Dichlorodihydrofluorescein&rft.jtitle=Bio-protocol&rft.au=Dornas,%20Waleska&rft.date=2021-12-20&rft.volume=11&rft.issue=24&rft.spage=e4279&rft.epage=e4279&rft.pages=e4279-e4279&rft.issn=2331-8325&rft.eissn=2331-8325&rft_id=info:doi/10.21769/BioProtoc.4279&rft_dat=%3Cproquest_pubme%3E2623880269%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2623880269&rft_id=info:pmid/35087931&rfr_iscdi=true