On-Tissue Derivatization of Lipopolysaccharide for Detection of Lipid A Using MALDI-MSI

We developed a method to directly detect and map the Gram-negative bacterial virulence factor lipid A derived from lipopolysaccharide (LPS) by coupling acid hydrolysis with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). As the structure of lipid A (endotoxin) dete...

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Veröffentlicht in:	Analytical chemistry (Washington) 2020-10, Vol.92 (20), p.13667-13671
Hauptverfasser:	Yang, Hyojik, Chandler, Courtney E, Jackson, Shelley N, Woods, Amina S, Goodlett, David R, Ernst, Robert K, Scott, Alison J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Antigens Antimicrobial resistance Bacterial diseases Bacterial infections Chemistry Coupling (molecular) E coli Endotoxins Escherichia coli - metabolism Gram-negative bacteria Hydrolysis Inflammation Ionization Ions Kidney - microbiology Limit of Detection Lipid A Lipid A - analysis Lipids Lipopolysaccharides Lipopolysaccharides - metabolism Mass spectrometry Mass spectroscopy Mice Pathogens Phenotypes Polysaccharides Pseudomonas aeruginosa Pseudomonas aeruginosa - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Substrates Tissues Virulence Virulence factors
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