Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells
The characteristics of retrograde filling material are important factors that can affect the long-term success of apical microsurgery. Various calcium silicate-based cements (CSC) were introduced to overcome drawbacks of mineral trioxide aggregate (MTA), while Emdogain is known to be effective in th...
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| description | The characteristics of retrograde filling material are important factors that can affect the long-term success of apical microsurgery. Various calcium silicate-based cements (CSC) were introduced to overcome drawbacks of mineral trioxide aggregate (MTA), while Emdogain is known to be effective in the regeneration of periodontal tissues. The aim of this study is to evaluate the biocompatibility and osteogenic potential of various CSCs combined with Emdogain on human bone marrow-derived mesenchymal stem cells. Experimental groups were classified into eight groups depending on the material and the presence of Emdogain. In the cell-counting kit test, all experimental groups combined with Emdogain showed higher cell viability compared with those without Emdogain at days 1 and 2. In the wound-healing assay, cell migration increased significantly over time, with or without Emdogain. In the alkaline phosphatase assay, all groups treated with Emdogain showed higher activity compared with those without Emdogain at day 3 (
< 0.05). Using alizarin red S staining, all groups treated with Emdogain showed greater calcium nodule formation compared with those without Emdogain at days 7 and 14 (
< 0.05). In conclusion, using CSCs as retrograde filling materials and the application of additional Emdogain will increase bone regeneration and improve the prognosis of apical microsurgery. |
| doi_str_mv | 10.3390/ma14247750 |
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< 0.05). Using alizarin red S staining, all groups treated with Emdogain showed greater calcium nodule formation compared with those without Emdogain at days 7 and 14 (
< 0.05). In conclusion, using CSCs as retrograde filling materials and the application of additional Emdogain will increase bone regeneration and improve the prognosis of apical microsurgery.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma14247750</identifier><identifier>PMID: 34947344</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Alizarin ; Alkaline phosphatase ; Biocompatibility ; Biomedical materials ; Bone marrow ; Calcium silicates ; Cell adhesion & migration ; Cell culture ; Cements ; Fillers ; Heavy metal content ; Microsurgery ; Phosphatase ; Regeneration (physiology) ; Stem cells ; Surgery ; Wound healing</subject><ispartof>Materials, 2021-12, Vol.14 (24), p.7750</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-309460110709d00828e29ce7b339560ff4cb786641b6eb324e6c8ec72fef5ac33</citedby><cites>FETCH-LOGICAL-c406t-309460110709d00828e29ce7b339560ff4cb786641b6eb324e6c8ec72fef5ac33</cites><orcidid>0000-0002-9894-5453</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706689/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8706689/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34947344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hye-Min</creatorcontrib><creatorcontrib>Lee, Donghee</creatorcontrib><creatorcontrib>Kim, Sin-Young</creatorcontrib><title>Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells</title><title>Materials</title><addtitle>Materials (Basel)</addtitle><description>The characteristics of retrograde filling material are important factors that can affect the long-term success of apical microsurgery. Various calcium silicate-based cements (CSC) were introduced to overcome drawbacks of mineral trioxide aggregate (MTA), while Emdogain is known to be effective in the regeneration of periodontal tissues. The aim of this study is to evaluate the biocompatibility and osteogenic potential of various CSCs combined with Emdogain on human bone marrow-derived mesenchymal stem cells. Experimental groups were classified into eight groups depending on the material and the presence of Emdogain. In the cell-counting kit test, all experimental groups combined with Emdogain showed higher cell viability compared with those without Emdogain at days 1 and 2. In the wound-healing assay, cell migration increased significantly over time, with or without Emdogain. In the alkaline phosphatase assay, all groups treated with Emdogain showed higher activity compared with those without Emdogain at day 3 (
< 0.05). Using alizarin red S staining, all groups treated with Emdogain showed greater calcium nodule formation compared with those without Emdogain at days 7 and 14 (
< 0.05). In conclusion, using CSCs as retrograde filling materials and the application of additional Emdogain will increase bone regeneration and improve the prognosis of apical microsurgery.</description><subject>Alizarin</subject><subject>Alkaline phosphatase</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bone marrow</subject><subject>Calcium silicates</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cements</subject><subject>Fillers</subject><subject>Heavy metal content</subject><subject>Microsurgery</subject><subject>Phosphatase</subject><subject>Regeneration (physiology)</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Wound healing</subject><issn>1996-1944</issn><issn>1996-1944</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkd9OFDEYxSdGIgS58QFME28MyWj_TWfqhYk7LkKCwQS9bjrdb6Bk2q5tZ4HX8UnpCiLam7bpL-c7p6eqXhH8jjGJ3ztNOOVt2-Bn1R6RUtREcv78yXm3OkjpCpfFGOmofFHtMi55yzjfq34tbDDBrXW2g51svkXar9BZyhAuwFuDvoUMPls9oTCiXk_Gzg6dF9ToDPVCJ1ihHlxhUB_cYH25X9t8iZZeO5jQV52jvUGfIdpNGbKBD2g5jmByQsGj49lpjxbBQwFjDNf1b7BonGdwRXia0stqZ9RTgoOHfb_6cbT83h_Xp2dfTvpPp7XhWOSaYckFJgS3WK4w7mgHVBpoh_JNjcDjyM3QdkJwMggYGOUgTAempSOMjTaM7Vcf73XX8-BgZUqkqCe1jtbpeKuCturfF28v1UXYqK7FQnSyCLx9EIjh5wwpK2eTKRG0hzAnRUWpilFJmoK--Q-9CnP0Jd6Woh2WtNk6OrynTAwpRRgfzRCstu2rv-0X-PVT-4_on67ZHciIq94</recordid><startdate>20211215</startdate><enddate>20211215</enddate><creator>Kim, Hye-Min</creator><creator>Lee, Donghee</creator><creator>Kim, Sin-Young</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9894-5453</orcidid></search><sort><creationdate>20211215</creationdate><title>Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells</title><author>Kim, Hye-Min ; Lee, Donghee ; Kim, Sin-Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-309460110709d00828e29ce7b339560ff4cb786641b6eb324e6c8ec72fef5ac33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alizarin</topic><topic>Alkaline phosphatase</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Bone marrow</topic><topic>Calcium silicates</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cements</topic><topic>Fillers</topic><topic>Heavy metal content</topic><topic>Microsurgery</topic><topic>Phosphatase</topic><topic>Regeneration (physiology)</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hye-Min</creatorcontrib><creatorcontrib>Lee, Donghee</creatorcontrib><creatorcontrib>Kim, Sin-Young</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hye-Min</au><au>Lee, Donghee</au><au>Kim, Sin-Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells</atitle><jtitle>Materials</jtitle><addtitle>Materials (Basel)</addtitle><date>2021-12-15</date><risdate>2021</risdate><volume>14</volume><issue>24</issue><spage>7750</spage><pages>7750-</pages><issn>1996-1944</issn><eissn>1996-1944</eissn><abstract>The characteristics of retrograde filling material are important factors that can affect the long-term success of apical microsurgery. Various calcium silicate-based cements (CSC) were introduced to overcome drawbacks of mineral trioxide aggregate (MTA), while Emdogain is known to be effective in the regeneration of periodontal tissues. The aim of this study is to evaluate the biocompatibility and osteogenic potential of various CSCs combined with Emdogain on human bone marrow-derived mesenchymal stem cells. Experimental groups were classified into eight groups depending on the material and the presence of Emdogain. In the cell-counting kit test, all experimental groups combined with Emdogain showed higher cell viability compared with those without Emdogain at days 1 and 2. In the wound-healing assay, cell migration increased significantly over time, with or without Emdogain. In the alkaline phosphatase assay, all groups treated with Emdogain showed higher activity compared with those without Emdogain at day 3 (
< 0.05). Using alizarin red S staining, all groups treated with Emdogain showed greater calcium nodule formation compared with those without Emdogain at days 7 and 14 (
< 0.05). In conclusion, using CSCs as retrograde filling materials and the application of additional Emdogain will increase bone regeneration and improve the prognosis of apical microsurgery.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34947344</pmid><doi>10.3390/ma14247750</doi><orcidid>https://orcid.org/0000-0002-9894-5453</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alizarin Alkaline phosphatase Biocompatibility Biomedical materials Bone marrow Calcium silicates Cell adhesion & migration Cell culture Cements Fillers Heavy metal content Microsurgery Phosphatase Regeneration (physiology) Stem cells Surgery Wound healing |
| title | Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells |
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