Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation
Persistent infection with high-risk human papillomavirus (HPV) is the main risk factor for cervical cancer. Our mass spectrometry data showed that the Ras-associated binding protein Rab31 was upregulated by HPV; however, little is known regarding the role of Rab31 in the metastasis of cervical cance...
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| Veröffentlicht in: | International journal of biological sciences 2022, Vol.18 (1), p.112-123 |
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| creator | Huang, Yujie Liu, Ruijuan Han, Xuechao Hou, Xiaoyan Tian, Yonghao Zhang, Weifang |
| description | Persistent infection with high-risk human papillomavirus (HPV) is the main risk factor for cervical cancer. Our mass spectrometry data showed that the Ras-associated binding protein Rab31 was upregulated by HPV; however, little is known regarding the role of Rab31 in the metastasis of cervical cancer cells. In this study, we showed that Rab31 was highly expressed in cervical cancer tissues and cells, and both HPV E6 and E7 promoted the expression of Rab31. Rab31 knockdown inhibited while Rab31 overexpression promoted the migration and invasion capabilities of cervical cancer cells. Additionally, Rab31 knockdown inhibited the epithelial-mesenchymal transition (EMT) and cytoskeletal rearrangement in cervical cancer cells. Furthermore, Rab31 interacted with mitogen-activated protein kinase 6 (MAPK6), and Rab31 knockdown inhibited the expression of MAPK6, which was mainly localized in the cytoplasm. More importantly, Rab31 knockdown promoted and Rab31 overexpression inhibited MAPK6 degradation. Accordingly, MAPK6 overexpression restored the decreased migration potential caused by Rab31 knockdown. Finally, a xenograft mouse model showed that Rab31 knockdown in cervical cancer cells led to reduced tumor growth and impaired lung and liver metastasis
. In conclusion, Rab31 plays a crucial role in cervical cancer metastasis by inhibiting MAPK6 degradation. Thus, Rab31 may serve as a novel therapeutic target to manage cervical cancer. |
| doi_str_mv | 10.7150/ijbs.63388 |
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. In conclusion, Rab31 plays a crucial role in cervical cancer metastasis by inhibiting MAPK6 degradation. Thus, Rab31 may serve as a novel therapeutic target to manage cervical cancer.</description><identifier>ISSN: 1449-2288</identifier><identifier>EISSN: 1449-2288</identifier><identifier>DOI: 10.7150/ijbs.63388</identifier><identifier>PMID: 34975321</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher Pty Ltd</publisher><subject>Animals ; Antibodies ; Cancer ; Cell adhesion & migration ; Cell Movement ; Cervical cancer ; Cervix ; Cytoplasm ; Cytoskeleton ; Degradation ; Disease Progression ; Experiments ; Female ; HaCaT Cells ; Health risks ; HeLa Cells ; Human papillomavirus ; Humans ; Infections ; Kinases ; MAP kinase ; Mass spectrometry ; Mass spectroscopy ; Medical prognosis ; Mesenchyme ; Metastases ; Metastasis ; Mice ; Mice, Inbred BALB C ; Mitogen-Activated Protein Kinase 6 - metabolism ; Protein kinase ; Proteins ; rab GTP-Binding Proteins - metabolism ; Research Paper ; Risk analysis ; Risk factors ; Therapeutic targets ; Up-Regulation ; Uterine Cervical Neoplasms - metabolism ; Uterine Cervical Neoplasms - pathology ; Wound healing ; Xenograft Model Antitumor Assays ; Xenografts ; Xenotransplantation</subject><ispartof>International journal of biological sciences, 2022, Vol.18 (1), p.112-123</ispartof><rights>The author(s).</rights><rights>2022. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The author(s) 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-ac46397f0f9d7ca4dd8e579989d916cdde2c608c74ba3340585e97829b509d663</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692139/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692139/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34975321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yujie</creatorcontrib><creatorcontrib>Liu, Ruijuan</creatorcontrib><creatorcontrib>Han, Xuechao</creatorcontrib><creatorcontrib>Hou, Xiaoyan</creatorcontrib><creatorcontrib>Tian, Yonghao</creatorcontrib><creatorcontrib>Zhang, Weifang</creatorcontrib><title>Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation</title><title>International journal of biological sciences</title><addtitle>Int J Biol Sci</addtitle><description>Persistent infection with high-risk human papillomavirus (HPV) is the main risk factor for cervical cancer. Our mass spectrometry data showed that the Ras-associated binding protein Rab31 was upregulated by HPV; however, little is known regarding the role of Rab31 in the metastasis of cervical cancer cells. In this study, we showed that Rab31 was highly expressed in cervical cancer tissues and cells, and both HPV E6 and E7 promoted the expression of Rab31. Rab31 knockdown inhibited while Rab31 overexpression promoted the migration and invasion capabilities of cervical cancer cells. Additionally, Rab31 knockdown inhibited the epithelial-mesenchymal transition (EMT) and cytoskeletal rearrangement in cervical cancer cells. Furthermore, Rab31 interacted with mitogen-activated protein kinase 6 (MAPK6), and Rab31 knockdown inhibited the expression of MAPK6, which was mainly localized in the cytoplasm. More importantly, Rab31 knockdown promoted and Rab31 overexpression inhibited MAPK6 degradation. Accordingly, MAPK6 overexpression restored the decreased migration potential caused by Rab31 knockdown. Finally, a xenograft mouse model showed that Rab31 knockdown in cervical cancer cells led to reduced tumor growth and impaired lung and liver metastasis
. In conclusion, Rab31 plays a crucial role in cervical cancer metastasis by inhibiting MAPK6 degradation. Thus, Rab31 may serve as a novel therapeutic target to manage cervical cancer.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Cancer</subject><subject>Cell adhesion & migration</subject><subject>Cell Movement</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Cytoplasm</subject><subject>Cytoskeleton</subject><subject>Degradation</subject><subject>Disease Progression</subject><subject>Experiments</subject><subject>Female</subject><subject>HaCaT Cells</subject><subject>Health risks</subject><subject>HeLa Cells</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Infections</subject><subject>Kinases</subject><subject>MAP kinase</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical prognosis</subject><subject>Mesenchyme</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mitogen-Activated Protein Kinase 6 - metabolism</subject><subject>Protein kinase</subject><subject>Proteins</subject><subject>rab GTP-Binding Proteins - metabolism</subject><subject>Research Paper</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>Therapeutic targets</subject><subject>Up-Regulation</subject><subject>Uterine Cervical Neoplasms - metabolism</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Wound healing</subject><subject>Xenograft Model Antitumor Assays</subject><subject>Xenografts</subject><subject>Xenotransplantation</subject><issn>1449-2288</issn><issn>1449-2288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkV9LHDEUxYNU1Nq-9AOUQF-KsJr_k7wIIrUtrliKPodMktnNMpOsyezCfnuzdSurcCH3kt89nMsB4AtG5w3m6CIs2nIuKJXyAJxgxtSEECk_7PXH4GMpC4So4BIdgWPKVMMpwSfA_jUtxXCZ05BGX-A49zDEtSkhRWiig4MfTakVCkwdtD6vgzU9tCbWvs59X2C7qTvz0IYxxBm8u_pzK6Dzs2ycGavOJ3DYmb74z7v3FDze_Hi4_jWZ3v_8fX01nViGxDgxlgmqmg51yjXWMOek541SUjmFhXXOEyuQtA1rDaUMccm9aiRRLUfKCUFPweWL7nLVDt5ZH8dser3MYTB5o5MJ-u1PDHM9S2sthSKYqirwfSeQ09PKl1EPoWxPNNGnVdFEYEEkk4pW9Ns7dJFWOdbzNOGKi2pUNpU6e6FsTqVk372awUhvs9Pb7PS_7Cr8dd_-K_o_LPoMDWeVog</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Huang, Yujie</creator><creator>Liu, Ruijuan</creator><creator>Han, Xuechao</creator><creator>Hou, Xiaoyan</creator><creator>Tian, Yonghao</creator><creator>Zhang, Weifang</creator><general>Ivyspring International Publisher Pty Ltd</general><general>Ivyspring International Publisher</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2022</creationdate><title>Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation</title><author>Huang, Yujie ; Liu, Ruijuan ; Han, Xuechao ; Hou, Xiaoyan ; Tian, Yonghao ; Zhang, Weifang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-ac46397f0f9d7ca4dd8e579989d916cdde2c608c74ba3340585e97829b509d663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Cancer</topic><topic>Cell adhesion & migration</topic><topic>Cell Movement</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Cytoplasm</topic><topic>Cytoskeleton</topic><topic>Degradation</topic><topic>Disease Progression</topic><topic>Experiments</topic><topic>Female</topic><topic>HaCaT Cells</topic><topic>Health risks</topic><topic>HeLa Cells</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Infections</topic><topic>Kinases</topic><topic>MAP kinase</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical prognosis</topic><topic>Mesenchyme</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mitogen-Activated Protein Kinase 6 - metabolism</topic><topic>Protein kinase</topic><topic>Proteins</topic><topic>rab GTP-Binding Proteins - metabolism</topic><topic>Research Paper</topic><topic>Risk analysis</topic><topic>Risk factors</topic><topic>Therapeutic targets</topic><topic>Up-Regulation</topic><topic>Uterine Cervical Neoplasms - metabolism</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Wound healing</topic><topic>Xenograft Model Antitumor Assays</topic><topic>Xenografts</topic><topic>Xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yujie</creatorcontrib><creatorcontrib>Liu, Ruijuan</creatorcontrib><creatorcontrib>Han, Xuechao</creatorcontrib><creatorcontrib>Hou, Xiaoyan</creatorcontrib><creatorcontrib>Tian, Yonghao</creatorcontrib><creatorcontrib>Zhang, Weifang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yujie</au><au>Liu, Ruijuan</au><au>Han, Xuechao</au><au>Hou, Xiaoyan</au><au>Tian, Yonghao</au><au>Zhang, Weifang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation</atitle><jtitle>International journal of biological sciences</jtitle><addtitle>Int J Biol Sci</addtitle><date>2022</date><risdate>2022</risdate><volume>18</volume><issue>1</issue><spage>112</spage><epage>123</epage><pages>112-123</pages><issn>1449-2288</issn><eissn>1449-2288</eissn><abstract>Persistent infection with high-risk human papillomavirus (HPV) is the main risk factor for cervical cancer. Our mass spectrometry data showed that the Ras-associated binding protein Rab31 was upregulated by HPV; however, little is known regarding the role of Rab31 in the metastasis of cervical cancer cells. In this study, we showed that Rab31 was highly expressed in cervical cancer tissues and cells, and both HPV E6 and E7 promoted the expression of Rab31. Rab31 knockdown inhibited while Rab31 overexpression promoted the migration and invasion capabilities of cervical cancer cells. Additionally, Rab31 knockdown inhibited the epithelial-mesenchymal transition (EMT) and cytoskeletal rearrangement in cervical cancer cells. Furthermore, Rab31 interacted with mitogen-activated protein kinase 6 (MAPK6), and Rab31 knockdown inhibited the expression of MAPK6, which was mainly localized in the cytoplasm. More importantly, Rab31 knockdown promoted and Rab31 overexpression inhibited MAPK6 degradation. Accordingly, MAPK6 overexpression restored the decreased migration potential caused by Rab31 knockdown. Finally, a xenograft mouse model showed that Rab31 knockdown in cervical cancer cells led to reduced tumor growth and impaired lung and liver metastasis
. In conclusion, Rab31 plays a crucial role in cervical cancer metastasis by inhibiting MAPK6 degradation. Thus, Rab31 may serve as a novel therapeutic target to manage cervical cancer.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher Pty Ltd</pub><pmid>34975321</pmid><doi>10.7150/ijbs.63388</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies Cancer Cell adhesion & migration Cell Movement Cervical cancer Cervix Cytoplasm Cytoskeleton Degradation Disease Progression Experiments Female HaCaT Cells Health risks HeLa Cells Human papillomavirus Humans Infections Kinases MAP kinase Mass spectrometry Mass spectroscopy Medical prognosis Mesenchyme Metastases Metastasis Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 6 - metabolism Protein kinase Proteins rab GTP-Binding Proteins - metabolism Research Paper Risk analysis Risk factors Therapeutic targets Up-Regulation Uterine Cervical Neoplasms - metabolism Uterine Cervical Neoplasms - pathology Wound healing Xenograft Model Antitumor Assays Xenografts Xenotransplantation |
| title | Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation |
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