Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain
Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (...
Gespeichert in:
| Veröffentlicht in: | IET nanobiotechnology 2019-12, Vol.13 (9), p.967-973 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 973 |
|---|---|
| container_issue | 9 |
| container_start_page | 967 |
| container_title | IET nanobiotechnology |
| container_volume | 13 |
| creator | Das, Moonjit Borah, Debajit Patowary, Kaustuvmani Borah, Mayuri Khataniar, Ankita Bhusan Kakoti, Bibhuti |
| description | Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (AgNPs) having antimicrobial and non-cytotoxic activities with the help of microbial biosurfactant extracted from a novel Bacillus vallismortis strain MDU6 (Genbank accession no. MH382951) from petroleum oil logged soil sample in Dibrugarh, Assam. The isolate shows excellent potential for the production of biosurfactant by reducing the surface tension of diesel supplemented medium up to 56.57% only within 5 days. FTIR spectra of the crude biosurfactant show the presence of ʋCH2 (asymmetric stretching), ʋCH2 (symmetric stretching), ʋC=C (stretch), ʋC−C (stretch), ʋC−H (bending), ʋC−O (stretch) and ʋC−H (bending) functional groups and LC-MS/MS analysis confirms it as a cyclic lipopeptide which is a mixture of surfactin and iturin. The synthesized AgNPs showed excellent antimicrobial activities against Escherichia coli (ATCC no. 25922), Listeria monocytogenes (ATCC No. BAA-751), Staphylococcus aureus (ATCC No. 9542) and Bacillus subtilis (ATCC no. 6051) and showed no cytotoxicity against primary mouse liver cell lines. |
| doi_str_mv | 10.1049/iet-nbt.2019.0038 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8676646</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>NBT2BF00647</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5227-35c861c6993c544b661383e8717959defccf94b8f4348a825454ef12d8fdbbeb3</originalsourceid><addsrcrecordid>eNqFkU9vFCEchidGY2v1A3gxXD3MCjPAMB5Muv2jTapetok3Agy0NCxsgNlmvoqfViaja000niDwPO-P8FbVawRXCOL-ndW59jKvGoj6FYQte1Ido46gmnXk29PDHqOj6kVK9xASQlr2vDpqEUOoo-y4-n7qs91aFYO0wgGhst3bPIFgQLJuryPwwoediNkqpxNIk893OtmkByAnMCbrb8FvX9qQxmhKjPAZ7GIYRrWQAnj94CZgU3Ail7O1UNa5MYG9cM6mbSgjEvh8fkNBylFY_7J6ZoRL-tXP9aS6ubzYnH2qr79-vDo7va4VaZquboliFCna960iGEtKUctazTrU9aQftFHK9Fgyg1vMBGsIJlgb1AzMDFJq2Z5UH5bc3Si3elDal_GO76LdijjxICz_88bbO34b9pzRjlJMSwBaAsovpBS1ObgI8rkoXoripSg-F8Xnoorz5vHQg_GrmQK8X4AH6_T0_0T-Zb1p1pcQUtwV-e0iz9h9GKMvH8ivLjYz9cjZDaaw9V_Yf7_8B-y_xTA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain</title><source>Wiley-Blackwell Open Access Titles(OpenAccess)</source><creator>Das, Moonjit ; Borah, Debajit ; Patowary, Kaustuvmani ; Borah, Mayuri ; Khataniar, Ankita ; Bhusan Kakoti, Bibhuti</creator><creatorcontrib>Das, Moonjit ; Borah, Debajit ; Patowary, Kaustuvmani ; Borah, Mayuri ; Khataniar, Ankita ; Bhusan Kakoti, Bibhuti</creatorcontrib><description>Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (AgNPs) having antimicrobial and non-cytotoxic activities with the help of microbial biosurfactant extracted from a novel Bacillus vallismortis strain MDU6 (Genbank accession no. MH382951) from petroleum oil logged soil sample in Dibrugarh, Assam. The isolate shows excellent potential for the production of biosurfactant by reducing the surface tension of diesel supplemented medium up to 56.57% only within 5 days. FTIR spectra of the crude biosurfactant show the presence of ʋCH2 (asymmetric stretching), ʋCH2 (symmetric stretching), ʋC=C (stretch), ʋC−C (stretch), ʋC−H (bending), ʋC−O (stretch) and ʋC−H (bending) functional groups and LC-MS/MS analysis confirms it as a cyclic lipopeptide which is a mixture of surfactin and iturin. The synthesized AgNPs showed excellent antimicrobial activities against Escherichia coli (ATCC no. 25922), Listeria monocytogenes (ATCC No. BAA-751), Staphylococcus aureus (ATCC No. 9542) and Bacillus subtilis (ATCC no. 6051) and showed no cytotoxicity against primary mouse liver cell lines.</description><identifier>ISSN: 1751-8741</identifier><identifier>ISSN: 1751-875X</identifier><identifier>EISSN: 1751-875X</identifier><identifier>DOI: 10.1049/iet-nbt.2019.0038</identifier><identifier>PMID: 31811768</identifier><language>eng</language><publisher>United States: The Institution of Engineering and Technology</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; antibacterial activity ; antimicrobial activity ; asymmetric stretching ; ATCC no ; Bacillus - metabolism ; bacillus subtilis ; bacillus vallismortis MDU6 strain ; Bacteria - drug effects ; bending ; biotechnology ; cellular biophysics ; chemical diversity ; crude biosurfactant show ; C−H functional groups ; environmental sectors ; excellent antimicrobial activities ; Fourier transform infrared spectra ; Genbank accession no. MH382951 ; green molecules ; industrial sectors ; isolate ; liver ; Metal Nanoparticles ; Mice ; microbial biosurfactants ; Microbial Sensitivity Tests ; microorganisms ; molecular biophysics ; nanofabrication ; nanomedicine ; nanoparticles ; noncytotoxic activities ; petroleum ; petroleum oil ; pharmaceutical sector ; Research Article ; silver ; Silver - chemistry ; silver nanoparticles ; soil sample ; Spectroscopy, Fourier Transform Infrared ; surface tension ; Surface-Active Agents - metabolism ; surfactants ; symmetric stretching ; synthesised AgNPs ; time 5.0 d ; toxicology</subject><ispartof>IET nanobiotechnology, 2019-12, Vol.13 (9), p.967-973</ispartof><rights>The Institution of Engineering and Technology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5227-35c861c6993c544b661383e8717959defccf94b8f4348a825454ef12d8fdbbeb3</citedby><cites>FETCH-LOGICAL-c5227-35c861c6993c544b661383e8717959defccf94b8f4348a825454ef12d8fdbbeb3</cites><orcidid>0000-0002-9367-5589</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676646/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8676646/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1049%2Fiet-nbt.2019.0038$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31811768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Das, Moonjit</creatorcontrib><creatorcontrib>Borah, Debajit</creatorcontrib><creatorcontrib>Patowary, Kaustuvmani</creatorcontrib><creatorcontrib>Borah, Mayuri</creatorcontrib><creatorcontrib>Khataniar, Ankita</creatorcontrib><creatorcontrib>Bhusan Kakoti, Bibhuti</creatorcontrib><title>Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain</title><title>IET nanobiotechnology</title><addtitle>IET Nanobiotechnol</addtitle><description>Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (AgNPs) having antimicrobial and non-cytotoxic activities with the help of microbial biosurfactant extracted from a novel Bacillus vallismortis strain MDU6 (Genbank accession no. MH382951) from petroleum oil logged soil sample in Dibrugarh, Assam. The isolate shows excellent potential for the production of biosurfactant by reducing the surface tension of diesel supplemented medium up to 56.57% only within 5 days. FTIR spectra of the crude biosurfactant show the presence of ʋCH2 (asymmetric stretching), ʋCH2 (symmetric stretching), ʋC=C (stretch), ʋC−C (stretch), ʋC−H (bending), ʋC−O (stretch) and ʋC−H (bending) functional groups and LC-MS/MS analysis confirms it as a cyclic lipopeptide which is a mixture of surfactin and iturin. The synthesized AgNPs showed excellent antimicrobial activities against Escherichia coli (ATCC no. 25922), Listeria monocytogenes (ATCC No. BAA-751), Staphylococcus aureus (ATCC No. 9542) and Bacillus subtilis (ATCC no. 6051) and showed no cytotoxicity against primary mouse liver cell lines.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibacterial activity</subject><subject>antimicrobial activity</subject><subject>asymmetric stretching</subject><subject>ATCC no</subject><subject>Bacillus - metabolism</subject><subject>bacillus subtilis</subject><subject>bacillus vallismortis MDU6 strain</subject><subject>Bacteria - drug effects</subject><subject>bending</subject><subject>biotechnology</subject><subject>cellular biophysics</subject><subject>chemical diversity</subject><subject>crude biosurfactant show</subject><subject>C−H functional groups</subject><subject>environmental sectors</subject><subject>excellent antimicrobial activities</subject><subject>Fourier transform infrared spectra</subject><subject>Genbank accession no. MH382951</subject><subject>green molecules</subject><subject>industrial sectors</subject><subject>isolate</subject><subject>liver</subject><subject>Metal Nanoparticles</subject><subject>Mice</subject><subject>microbial biosurfactants</subject><subject>Microbial Sensitivity Tests</subject><subject>microorganisms</subject><subject>molecular biophysics</subject><subject>nanofabrication</subject><subject>nanomedicine</subject><subject>nanoparticles</subject><subject>noncytotoxic activities</subject><subject>petroleum</subject><subject>petroleum oil</subject><subject>pharmaceutical sector</subject><subject>Research Article</subject><subject>silver</subject><subject>Silver - chemistry</subject><subject>silver nanoparticles</subject><subject>soil sample</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>surface tension</subject><subject>Surface-Active Agents - metabolism</subject><subject>surfactants</subject><subject>symmetric stretching</subject><subject>synthesised AgNPs</subject><subject>time 5.0 d</subject><subject>toxicology</subject><issn>1751-8741</issn><issn>1751-875X</issn><issn>1751-875X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vFCEchidGY2v1A3gxXD3MCjPAMB5Muv2jTapetok3Agy0NCxsgNlmvoqfViaja000niDwPO-P8FbVawRXCOL-ndW59jKvGoj6FYQte1Ido46gmnXk29PDHqOj6kVK9xASQlr2vDpqEUOoo-y4-n7qs91aFYO0wgGhst3bPIFgQLJuryPwwoediNkqpxNIk893OtmkByAnMCbrb8FvX9qQxmhKjPAZ7GIYRrWQAnj94CZgU3Ail7O1UNa5MYG9cM6mbSgjEvh8fkNBylFY_7J6ZoRL-tXP9aS6ubzYnH2qr79-vDo7va4VaZquboliFCna960iGEtKUctazTrU9aQftFHK9Fgyg1vMBGsIJlgb1AzMDFJq2Z5UH5bc3Si3elDal_GO76LdijjxICz_88bbO34b9pzRjlJMSwBaAsovpBS1ObgI8rkoXoripSg-F8Xnoorz5vHQg_GrmQK8X4AH6_T0_0T-Zb1p1pcQUtwV-e0iz9h9GKMvH8ivLjYz9cjZDaaw9V_Yf7_8B-y_xTA</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Das, Moonjit</creator><creator>Borah, Debajit</creator><creator>Patowary, Kaustuvmani</creator><creator>Borah, Mayuri</creator><creator>Khataniar, Ankita</creator><creator>Bhusan Kakoti, Bibhuti</creator><general>The Institution of Engineering and Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9367-5589</orcidid></search><sort><creationdate>201912</creationdate><title>Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain</title><author>Das, Moonjit ; Borah, Debajit ; Patowary, Kaustuvmani ; Borah, Mayuri ; Khataniar, Ankita ; Bhusan Kakoti, Bibhuti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5227-35c861c6993c544b661383e8717959defccf94b8f4348a825454ef12d8fdbbeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibacterial activity</topic><topic>antimicrobial activity</topic><topic>asymmetric stretching</topic><topic>ATCC no</topic><topic>Bacillus - metabolism</topic><topic>bacillus subtilis</topic><topic>bacillus vallismortis MDU6 strain</topic><topic>Bacteria - drug effects</topic><topic>bending</topic><topic>biotechnology</topic><topic>cellular biophysics</topic><topic>chemical diversity</topic><topic>crude biosurfactant show</topic><topic>C−H functional groups</topic><topic>environmental sectors</topic><topic>excellent antimicrobial activities</topic><topic>Fourier transform infrared spectra</topic><topic>Genbank accession no. MH382951</topic><topic>green molecules</topic><topic>industrial sectors</topic><topic>isolate</topic><topic>liver</topic><topic>Metal Nanoparticles</topic><topic>Mice</topic><topic>microbial biosurfactants</topic><topic>Microbial Sensitivity Tests</topic><topic>microorganisms</topic><topic>molecular biophysics</topic><topic>nanofabrication</topic><topic>nanomedicine</topic><topic>nanoparticles</topic><topic>noncytotoxic activities</topic><topic>petroleum</topic><topic>petroleum oil</topic><topic>pharmaceutical sector</topic><topic>Research Article</topic><topic>silver</topic><topic>Silver - chemistry</topic><topic>silver nanoparticles</topic><topic>soil sample</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>surface tension</topic><topic>Surface-Active Agents - metabolism</topic><topic>surfactants</topic><topic>symmetric stretching</topic><topic>synthesised AgNPs</topic><topic>time 5.0 d</topic><topic>toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Moonjit</creatorcontrib><creatorcontrib>Borah, Debajit</creatorcontrib><creatorcontrib>Patowary, Kaustuvmani</creatorcontrib><creatorcontrib>Borah, Mayuri</creatorcontrib><creatorcontrib>Khataniar, Ankita</creatorcontrib><creatorcontrib>Bhusan Kakoti, Bibhuti</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>IET nanobiotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Das, Moonjit</au><au>Borah, Debajit</au><au>Patowary, Kaustuvmani</au><au>Borah, Mayuri</au><au>Khataniar, Ankita</au><au>Bhusan Kakoti, Bibhuti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain</atitle><jtitle>IET nanobiotechnology</jtitle><addtitle>IET Nanobiotechnol</addtitle><date>2019-12</date><risdate>2019</risdate><volume>13</volume><issue>9</issue><spage>967</spage><epage>973</epage><pages>967-973</pages><issn>1751-8741</issn><issn>1751-875X</issn><eissn>1751-875X</eissn><abstract>Microbial biosurfactants has evolved as green molecules and their chemical diversity has gained momentum in recent time not only in the field of environmental and industrial sectors but also in the pharmaceutical sector. In this study, an effort was made for the biosynthesis of silver nanoparticle (AgNPs) having antimicrobial and non-cytotoxic activities with the help of microbial biosurfactant extracted from a novel Bacillus vallismortis strain MDU6 (Genbank accession no. MH382951) from petroleum oil logged soil sample in Dibrugarh, Assam. The isolate shows excellent potential for the production of biosurfactant by reducing the surface tension of diesel supplemented medium up to 56.57% only within 5 days. FTIR spectra of the crude biosurfactant show the presence of ʋCH2 (asymmetric stretching), ʋCH2 (symmetric stretching), ʋC=C (stretch), ʋC−C (stretch), ʋC−H (bending), ʋC−O (stretch) and ʋC−H (bending) functional groups and LC-MS/MS analysis confirms it as a cyclic lipopeptide which is a mixture of surfactin and iturin. The synthesized AgNPs showed excellent antimicrobial activities against Escherichia coli (ATCC no. 25922), Listeria monocytogenes (ATCC No. BAA-751), Staphylococcus aureus (ATCC No. 9542) and Bacillus subtilis (ATCC no. 6051) and showed no cytotoxicity against primary mouse liver cell lines.</abstract><cop>United States</cop><pub>The Institution of Engineering and Technology</pub><pmid>31811768</pmid><doi>10.1049/iet-nbt.2019.0038</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-9367-5589</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1751-8741 |
| ispartof | IET nanobiotechnology, 2019-12, Vol.13 (9), p.967-973 |
| issn | 1751-8741 1751-875X 1751-875X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8676646 |
| source | Wiley-Blackwell Open Access Titles(OpenAccess) |
| subjects | Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology antibacterial activity antimicrobial activity asymmetric stretching ATCC no Bacillus - metabolism bacillus subtilis bacillus vallismortis MDU6 strain Bacteria - drug effects bending biotechnology cellular biophysics chemical diversity crude biosurfactant show C−H functional groups environmental sectors excellent antimicrobial activities Fourier transform infrared spectra Genbank accession no. MH382951 green molecules industrial sectors isolate liver Metal Nanoparticles Mice microbial biosurfactants Microbial Sensitivity Tests microorganisms molecular biophysics nanofabrication nanomedicine nanoparticles noncytotoxic activities petroleum petroleum oil pharmaceutical sector Research Article silver Silver - chemistry silver nanoparticles soil sample Spectroscopy, Fourier Transform Infrared surface tension Surface-Active Agents - metabolism surfactants symmetric stretching synthesised AgNPs time 5.0 d toxicology |
| title | Antimicrobial activity of silver nanoparticles synthesised by using microbial biosurfactant produced by a newly isolated Bacillus vallismortis MDU6 strain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A01%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_24P&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antimicrobial%20activity%20of%20silver%20nanoparticles%20synthesised%20by%20using%20microbial%20biosurfactant%20produced%20by%20a%20newly%20isolated%20Bacillus%20vallismortis%20MDU6%20strain&rft.jtitle=IET%20nanobiotechnology&rft.au=Das,%20Moonjit&rft.date=2019-12&rft.volume=13&rft.issue=9&rft.spage=967&rft.epage=973&rft.pages=967-973&rft.issn=1751-8741&rft.eissn=1751-875X&rft_id=info:doi/10.1049/iet-nbt.2019.0038&rft_dat=%3Cwiley_24P%3ENBT2BF00647%3C/wiley_24P%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/31811768&rfr_iscdi=true |